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Showing: 7621 - 7640 of 65936 results

  2. pBBR15.2-2H12.D11opt-scarRef

    Plasmid
    #221150
    Purpose
    cdGreen2.1 (optimized for Pseudomonas GC-content) expressed from a constitutive promoter in an operon with mScarlet-I (downstream) as a reference FP (Internal Lab ID: UJ11241)
    Depositor
    Urs Jenal
    Publication
    Kaczmarczyk et al Nat Commun. 2024 May 9;15(1):3920. doi:
    Inserts
    cdGreen2.1
    mScarlet-I
    Expression
    Bacterial
    Mutation
    N-terminus changed: starts with MSKKYGEAVIKE ...
    Promoter
    Constitutive and None (same as cdGreen2.1)
    Available since
    June 25, 2024
    Availability
    Academic Institutions and Nonprofits only
    221150-plasmid-map-sequence-id-437166

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  3. GLP1R-DuET

    Plasmid
    #213253
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Publication
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    GLP1R (GLP1R Human)
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Promoter
    CMV, T7
    Available since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    213253-plasmid-map-sequence-id-430803

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  4. piggyBac-rtTA (4th_Gen)-sfGFP-IRES-NGN2-puro (VK_1122)

    Plasmid
    #209079
    Purpose
    Ngn2 mediated cortical neuron induction, along with sfGFP over-expression
    Depositor
    Vikram Khurana
    Publication
    Lam et al Neuron. 2024 Sep 4;112(17):2886-2909.e16
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    sfGFP
    NGN2 (Neurog2 Mouse)
    Expression
    Mammalian
    Promoter
    TRE4G
    Available since
    Dec. 13, 2023
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    209079-plasmid-map-sequence-id-496201

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  5. pAAVS1_TREtight-GEM2-Halo-FRB-IRES-FKBP-SNAP-GFPNb_EF1a-rtTA3-T2A-Puro-P2A-Thy1.1

    Plasmid
    #197061
    Purpose
    Donor plasmid for human AAVS1 locus knock-in, doxycycline-inducible expression of GEM2-Halo-FRB and Adaptor Protein (FKBP-SNAP-vhhGFP4)
    Depositor
    Julia Mahamid
    Publication
    Fung et al Nat Methods. 2023 Dec;20(12):1900-1908.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    Encapsulin
    Adaptor Protein
    Use
    CRISPR
    Tags
    FRB and HaloTag
    Expression
    Mammalian
    Mutation
    Codon-optimised for human cell expression
    Available since
    Sept. 20, 2023
    Availability
    Academic Institutions and Nonprofits only
    197061-plasmid-map-sequence-id-402602

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  8. EGFP-p300

    Plasmid
    #191764
    Purpose
    Expression of EGFP fused to core p300 histone acetyltransferase
    Depositor
    Daniel Gerlich
    Publication
    Schneider et al Nature. 2022 Sep;609(7925):183-190. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    FRB-EGFP-p300 core (EP300 Human)
    Tags
    EGFP (N-terminal of p300 core) and FRB (N-termina…
    Expression
    Bacterial and Mammalian
    Promoter
    EF1alpha
    Available since
    Feb. 2, 2023
    Availability
    Academic Institutions and Nonprofits only
    191764-plasmid-map-sequence-id-383841

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  10. pSLQ10878

    Plasmid
    #183964
    Purpose
    CAG-hyperCas12a(nuclease active)-HA-P2A-mCherry-T2A-HygroR
    Depositor
    Stanley Qi
    Publication
    Guo et al Nat Cell Biol. 2022 Apr;24(4):590-600. d
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    HyperdCas12a
    Use
    CRISPR
    Tags
    HA, Hygromycin Resistance, and mCherry
    Expression
    Mammalian
    Mutation
    D156R, E292R, D235R, D350R
    Promoter
    CAG
    Available since
    June 1, 2022
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    183964-plasmid-map-sequence-id-363120

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  18. pDisplay-GRAB_ATP1.0mut-IRES-mCherry-CAAX

    Plasmid
    #167583
    Purpose
    Express the ATP-insensitive control sensor GRAB_ATP1.0mut in mammalian cells
    Depositor
    Yulong Li
    Publication
    Wu et al Neuron. 2021 Dec 17. pii: S0896-6273(21)
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    ATP-insensitive sensor GRAB_ATP1.0mut (control sensor) (P2RY1 Human)
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    April 21, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    167583-plasmid-map-sequence-id-330893

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  20. pJYDC1

    Plasmid
    #162458
    Purpose
    Yeast display plasmid for expression of C-terminal fusions with Aga2p yeast agglutinin. The reporter, eUnaG2 bilirubin dependent green-yellow fluorescent protein, is fused to the Nterminus
    Depositor
    Gideon Schreiber
    Publication
    Zahradník et al bioRxiv Preprint 2020
    Type
    Empty backbone
    Use
    Shuttle vector bacteria/yeast
    Tags
    HA tag, HDEL sequence, eUnaG2 fluorescent protein…
    Available since
    Feb. 19, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    4 Articles
    162458-plasmid-map-sequence-id-321474

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Showing: 7621 - 7640 of 65936 results