We narrowed to 17,336 results for: Por
-
Plasmid#191238PurposeExpresses nuclease A-H124L from Staphylococcus aureus, with an A112C mutation, fused through a flexible linker to the transmembrane domain of GpA and a His-tag, for fluorescent studies.DepositorInsertnuc (nuclease A), GYPA (GPA) (E(transmembrane)R)
Tagsnuclease A from S. aureus fused to the TM domain …ExpressionBacterialMutationChanged Alanine 112 to CysteinePromoterT7 promoterAvailable SinceDec. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
Transposon-for RMCE_Sleeping Beauty_LIR-CAG-Frt-red(katushka)-P2A-Puro-F3-PA-RIR
Plasmid#67275PurposeFor sleeping beauty transposition and establishment of an recombinase mediated cassette exchange docking site. To make RMCE in cell line instead of flp in cell linesDepositorInsertpuromycin resistant gene
Tagslinked to red flouresent protein gene (katushka)ExpressionMammalianPromoterCAGAvailable SinceJuly 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
AID-mTagBFP2-loxP_myo2_neoR-loxP
Plasmid#194055PurposeDual-selection cassette plasmid for knocking-in AID-mTagBFP2 into the C. elegans genomeDepositorAvailable SinceJan. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGGD_MiniTurboID-YFP
Plasmid#222434PurposeGolden Gate / Green Gate module for adding MiniTurboID and YFP as C-terminus of target protein.DepositorInsertMiniTurboID (BirA mutant)
UseGolden gate / green gate compatible cloning vectorTagsLinker and YFPMutationaa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, …Available SinceJuly 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDEST40-MCU-V5-HIS
Plasmid#31731DepositorAvailable SinceAug. 29, 2011AvailabilityAcademic Institutions and Nonprofits only -
LENTICRISPR-UPP1_sgRNA1
Plasmid#201634PurposeCRISPR/Cas9-mediated gene knock-outDepositorInsertUPP1 (UPP1 Human)
UseCRISPR and LentiviralAvailable SinceJune 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAH1 (lenti hLPL-FLAG)
Plasmid#102448PurposeFLAG-tagged human LPL in lentiviral vectorDepositorAvailable SinceMay 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pPonA-BI-Gl NORM-LacZA TER- LacZB
Plasmid#86212Purposebeta Globin NMD construct with and without PTC under Bi-directional Ponasterone A inducible promoterDepositorUsePonasterone a inducible promoterTagslacZA or lacZBExpressionMammalianMutation39TER in beta Globin in MCS IIPromoterPonasterone A inducibleAvailable SinceJan. 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
hPlekhm1-dsRedM
Plasmid#73592PurposeExpress human Plekhm1 in mammalian cellsDepositorAvailable SinceMarch 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLM-mCerulean-cMyc
Plasmid#23244DepositorInsertmCerulean-2A-MYC (MYC synthetic fluorescent protein, Human)
UseLentiviralAvailable SinceFeb. 5, 2010AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-Hpep1-betaArrestin2-T2A-mTagBFP2
Plasmid#194680PurposeCMV driven co-expression of peptide complementing GPCR recorder (beta-arrestin 2 fusion) and mTagBFP2DepositorInsertHpep1-betaArrestin2
ExpressionMammalianPromoterCMVAvailable SinceJan. 30, 2025AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-DRD2-cpHalo∆-mEGFP
Plasmid#194679PurposeCMV driven expression of D2R GPCR recorder in mammalian cell lines fused to mEGFPDepositorInsertDRD2_cpHalo∆
TagsmEGFPExpressionMammalianPromoterCMVAvailable SinceFeb. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBpaRS3tRNA
Plasmid#197102PurposeTo express the p-benzoylphenylalanine-specific variant of Methanocaldococcus jannaschii TyrRS and its cognate amber suppressor tRNA and allow UAG codon to be translated into p-benzoylphenylalanine.DepositorInsertTyrRS variant, amber suppressor tRNA
ExpressionBacterialMutationGly32, Ser107, Thr158, Ser159, Arg286 (TyrRS)Available SinceJune 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSynapsin-FLEX-soCoChR-GFP
Plasmid#107712PurposeA somatic channelrhodopsin, which enables single cell, single millisecond resolution optogenetics. Cre-dependent virusDepositorHas ServiceAAV9InsertsoCoChR-GFP
UseAAVTagsEGFP and KA2ExpressionMammalianPromoterhSynapsinAvailable SinceMay 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHR Grb2-TagBFP
Plasmid#188631PurposeHuman Grb2 C-terminally labeled with TagBFPDepositorAvailable SinceAug. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLM-mCherry-Klf4
Plasmid#23243DepositorInsertmCherry_2A_KLF4 (KLF4 synthetic fluorescent protein, Human)
UseLentiviralAvailable SinceFeb. 5, 2010AvailabilityAcademic Institutions and Nonprofits only -
TRE-UBC-hCD24-T2A-GFP
Plasmid#205456Purposelentiviral plasmid for expression of human CD24DepositorAvailable SinceSept. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
SOX17-3'gRNA
Plasmid#210465PurposegRNA targeting SOX17 3' terminal for CRISPR-Cas9-mediated knock-inDepositorInsertSOX17 (SOX17 Human)
UseCRISPRAvailable SinceJan. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCVL.SSA TLR (Sce)
Plasmid#45576PurposeCodes for the SSA-TLR with the target site for I-Sce I in a lentiviral backboneDepositorInserts5' iRFP arm
eGFP with I-Sce I TS
+3 mCherry
3' iRFP arm
UseLentiviralExpressionBacterial and MammalianMutationembedded I-Sce I TS from 163-185bp, truncated 25 …PromoterSFFVAvailable SinceNov. 4, 2013AvailabilityAcademic Institutions and Nonprofits only -
FUGW-RUSH-LAMP1-V5-APEX2
Plasmid#228524PurposeLentiviral vector, expresses Strep-KDEL-IRES-SBP-(Rat)LAMP1-V5-APEX2 in mammalian cells.DepositorInsertLAMP1 (Lamp1 Rat)
UseLentiviralTagsStreptavidin Binding Protein (SBP), Streptavidin-…ExpressionMammalianPromoterUbcAvailable SinceFeb. 27, 2025AvailabilityAcademic Institutions and Nonprofits only