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Addgene

We narrowed to 11,841 results for: cel.2

Showing: 11521 - 11550 of 11841 results
  1. pCS6-ZF(VEGFA)-StaPL(AI)-YFP-VPR

    Plasmid
    #111502
    Purpose
    Expresses a zinc finger specific to the human VEGFA locus, which is linked to a VPR transcriptional activation domain via a StaPL(AI) module, in mammalian cells. [AI = asunaprevir inhibited].
    Depositor
    Insert
    ZF(VEGFA)-StaPL(AI)-YFP-VPR
    Use
    Tags
    Expression
    Mammalian
    Mutation
    The HCV NS3 protease carries V36M, T54A, and S122…
    Promoter
    CMV
    Available since
    July 3, 2018
    Availability
    Academic Institutions and Nonprofits only
  2. pACEBac1-PSMB1-PSMB2-PSMB3-PSMB4-TEV-2xSTII

    Plasmid
    #217897
    Purpose
    Insect cell expression of human 20S CP proteasome subunit beta type-1 (PSMB1), beta type-2 (PSMB2), beta type-3 (PSMB3), and beta type-4 (PSMB4) with C-terminal Twin-Strep-tag
    Depositor
    Inserts
    PSMB1 (PSMB1 Human)
    PSMB2 (PSMB2 Human)
    PSMB3 (PSMB3 Human)
    PSMB4 (PSMB4 Human)
    Use
    Tags
    2xSTII
    Expression
    Insect
    Mutation
    Promoter
    Available since
    May 20, 2024
    Availability
    Academic Institutions and Nonprofits only
  3. LgBiT-KRAS(G12V)_SmBiT-CRAF-RBD Bidirectional Vector

    Plasmid
    #237067
    Purpose
    Express LgBiT-KRAS(G12V)_SmBiT-CRAF-RBD Fusion Protein in Mammalian Cells under a CMV promoter
    Has Service
    DNA
    Inserts
    KRAS (KRAS Human)
    cRAF(RBD) (RAF1 Human)
    Use
    Tags
    LgBiT and SmBiT
    Expression
    Mammalian
    Mutation
    G12V
    Promoter
    CMV
    Available since
    July 14, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
  4. LgBiT-KRAS (WT)_SmBiT-CRAF-RBD Bidirectional Vector

    Plasmid
    #237066
    Purpose
    Express LgBiT-KRAS (WT)_SmBiT-CRAF-RBD Fusion Protein in Mammalian Cells under a CMV promoter
    Has Service
    DNA
    Inserts
    KRAS (KRAS Human)
    cRAF(RBD) (RAF1 Human)
    Use
    Tags
    LgBiT and SmBiT
    Expression
    Mammalian
    Mutation
    None
    Promoter
    CMV
    Available since
    July 14, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
  5. cRAF CRD-RBD: KRAS G12C BiBRET Vector

    Plasmid
    #236872
    Purpose
    Express cRAF CRD-RBD: KRAS G12C in Mammalian Cells under a CMV promoter
    Has Service
    DNA
    Inserts
    KRAS (KRAS Human)
    cRAF(RBD)-CRD (RAF1 Human)
    Use
    Tags
    HaloTag (R) and NanoLuc (R)
    Expression
    Mammalian
    Mutation
    G12C
    Promoter
    CMV
    Available since
    July 14, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
  6. Nluc-KRAS(G12D)-cRAF(RBD)-HaloTag BiBRET Vector

    Plasmid
    #236867
    Purpose
    Express NanoLuc(R)-KRAS(G12D)-cRAF(RBD)-HaloTag(R) in Mammalian Cells under a CMV promoter
    Has Service
    DNA
    Inserts
    KRAS (KRAS Human)
    cRAF(RBD) (RAF1 Human)
    Use
    Tags
    HaloTag (R) and NanoLuc (R)
    Expression
    Mammalian
    Mutation
    G12D
    Promoter
    CMV
    Available since
    July 14, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
  7. Nluc-KRAS(G12C)-cRAF(FL)-HaloTag BiBRET Vector

    Plasmid
    #236868
    Purpose
    Express NanoLuc(R)-KRAS(G12C)-cRAF(FL)-HaloTag(R) in Mammalian Cells under a CMV promoter
    Has Service
    DNA
    Inserts
    KRAS (KRAS Human)
    cRAF (RAF1 Human)
    Use
    Tags
    HaloTag (R) and NanoLuc (R)
    Expression
    Mammalian
    Mutation
    G12C
    Promoter
    CMV
    Available since
    July 14, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
  8. Nluc-KRAS(G12C)-cRAF(RBD)-HaloTag BiBRET Vector

    Plasmid
    #236869
    Purpose
    Express NanoLuc(R)-KRAS(G12C)-cRAF(RBD)-HaloTag(R) in Mammalian Cells under a CMV promoter
    Has Service
    DNA
    Inserts
    KRAS (KRAS Human)
    cRAF(RBD) (RAF1 Human)
    Use
    Tags
    HaloTag (R) and NanoLuc (R)
    Expression
    Mammalian
    Mutation
    G12C
    Promoter
    CMV
    Available since
    July 14, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
  9. pMCh-1540

    Plasmid
    #82433
    Purpose
    Plasmid for expression of GST-tagged human-Nematostella GW182 11W11A chimera in mammalian cells
    Depositor
    Insert
    hs-nvGW182 11W11A chimera
    Use
    Tags
    GST
    Expression
    Mammalian
    Mutation
    aa 1-1169 of siRNA resistant hsTNRC6A (Q8NDV7-2) …
    Promoter
    EF1A
    Available since
    Sept. 20, 2016
    Availability
    Academic Institutions and Nonprofits only
  10. pPB-VPR-dSpCas9[StaPL(TI)]

    Plasmid
    #111508
    Purpose
    Expresses a VPR transcriptional activator fused to the nuclease-deactivated S. pyogenes Cas9, which is governed by an inserted StaPL(TI) module, in mammalian cells. [TI = telaprevir inhibited].
    Depositor
    Insert
    VPR-dSpCas9[StaPL(TI)]
    Use
    Tags
    Expression
    Mammalian
    Mutation
    The HCV NS3 protease carries F43L, Q80K, and S122…
    Promoter
    PGK
    Available since
    July 3, 2018
    Availability
    Academic Institutions and Nonprofits only
  11. pCEP4-PDGFRA-reporter

    Plasmid
    #136456
    Purpose
    Mammalian fluorescent reporter plasmid for PDGFRA signaling.
    Depositor
    Inserts
    PDGFRalpha (PDGFRA Human)
    Array of serum response elements (SRE) to drive destabilized GFP expression from a minimal promoter.
    Use
    Tags
    Extracellular c-myc epitope tag, Signal peptide f…
    Expression
    Mammalian
    Mutation
    Promoter
    CMV and Minimal promoter with artificial SRE enha…
    Available since
    July 9, 2020
    Availability
    Academic Institutions and Nonprofits only
  12. FLAG-FullLength-TET1-CXXC/LLAA

    Plasmid
    #124399
    Purpose
    Full-length mutant TET1 (L897A/L900A), missing zinc finger domain (585-624), used to PiggyBAC rescue the function of TET1/2 DKO cells
    Depositor
    Insert
    FLAG-FullLength-TET1 CXXC zinc finger deletion (585-624) and L897A/L900A mutations (TET1 Human)
    Use
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    CXXC zinc finger deletion (aa 585-624) and L897A/…
    Promoter
    Available since
    May 24, 2019
    Availability
    Academic Institutions and Nonprofits only
  13. pcs2-MTSmut-cox8A(XXXX)-GFP-IRES-mCherry

    Plasmid
    #226104
    Purpose
    Stability reporter construct of the COX8A mitochondrial targeting sequence (MTS) with mutations that impair recognition by SIFI for transient expression in mammalian cells
    Depositor
    Insert
    COX8A (COX8A Human)
    Use
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    encodes only for mitochondrial targeting sequence…
    Promoter
    Available since
    Oct. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
  14. pcDNA3-sPDGFRalpha-Fc-Y206S

    Plasmid
    #136439
    Purpose
    Mammalian expression plasmid for soluble ectodomain of PDGFR-alpha fused to IgG1 Fc (Y206S mutant)
    Depositor
    Insert
    PDGFRalpha (PDGFRA Human)
    Use
    Tags
    Fc region of IgG1 (a.a. Asp-221 to Lys-447) and L…
    Expression
    Mammalian
    Mutation
    Extracellular domain of PDGFR-alpha (a.a. 1-524) …
    Promoter
    CMV
    Available since
    July 9, 2020
    Availability
    Academic Institutions and Nonprofits only
  15. pcDNA3-sPDGFRalpha-Fc-V242K

    Plasmid
    #136440
    Purpose
    Mammalian expression plasmid for soluble ectodomain of PDGFR-alpha fused to IgG1 Fc (V242K mutant)
    Depositor
    Insert
    PDGFRalpha (PDGFRA Human)
    Use
    Tags
    Fc region of IgG1 (a.a. Asp-221 to Lys-447) and L…
    Expression
    Mammalian
    Mutation
    Extracellular domain of PDGFR-alpha (a.a. 1-524) …
    Promoter
    CMV
    Available since
    July 9, 2020
    Availability
    Academic Institutions and Nonprofits only
  16. pSCAR_Cas9-blast_GFP

    Plasmid
    #162074
    Purpose
    3rd generation lentivirus transfer vector for Cre-removable Cas9, GFP reporter, and blast resistance
    Depositor
    Inserts
    Cas9-2a-blasticidin S deaminase
    EGFP
    Use
    CRISPR, Cre/Lox, and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    codon-optimized for expression in M. musculus
    Promoter
    EF1a and sv40
    Available since
    Feb. 22, 2021
    Availability
    Academic Institutions and Nonprofits only
  17. pSCAR_Cas9-hygro_GFP

    Plasmid
    #162075
    Purpose
    3rd generation lentivirus transfer vector for Cre-removable Cas9, GFP reporter, and hygro resistance
    Depositor
    Inserts
    Cas9-2a-hygromycin B phosphotransferase
    EGFP
    Use
    CRISPR, Cre/Lox, and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    codon-optimized for expression in M. musculus
    Promoter
    EF1a and sv40
    Available since
    Feb. 22, 2021
    Availability
    Academic Institutions and Nonprofits only
Showing: 11521 - 11550 of 11841 results