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pBdox-SPI1-T2A-CEBPA-P2A-FLI1_BleoR

Plasmid

#250291

Purpose

PiggyBac integration of 3 transcription factors (SPI1, CEBPA, FLI1) under a dox-inducible promoter

Depositor

George Church

Article

Liu et al Nat Commun. 2025 Jun 10;16(1):5136. doi:

Insert

SPI1-T2A-CEBPA-P2A-FLI1

Expression

Mammalian

Available Since

March 31, 2026

Availability

Academic Institutions and Nonprofits only

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pBdox-MEF2C-T2A-CEBPB-P2A-IRF8_PuroR

Plasmid

#250292

Purpose

PiggyBac integration of 3 transcription factors (MEF2C, CEBPB, IRF8) under a dox-inducible promoter

Depositor

George Church

Article

Liu et al Nat Commun. 2025 Jun 10;16(1):5136. doi:

Insert

MEF2C-T2A-CEBPB-P2A-IRF8

Expression

Mammalian

Available Since

March 31, 2026

Availability

Academic Institutions and Nonprofits only

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pCDH-EF1a-eFFly-mCherry

Plasmid

#104833

Purpose

3rd generation lentiviral vector. eFFly luciferase for in vivo Imaging, mCherry for fluorescent detection

Depositor

Irmela Jeremias

Article

Ebinger et al Cancer Cell. 2016 Dec 12;30(6):849-862.

Inserts

eFFly
mCherry

Use

Lentiviral and Luciferase

Expression

Mammalian

Available Since

Aug. 20, 2018

Availability

Academic Institutions and Nonprofits only

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M31: pHR-SFFVp- TDP-43C::mCherry::SspB

Plasmid

#122669

Purpose

C terminal domain of TDP43 (218-414) tagged with SspB and mCherry.

Depositor

Clifford Brangwynne

Article

Bracha et al Cell. 2018 Nov 29;175(6):1467-1480.e13.

Insert

TDP43 (TARDBP Human)

Use

Lentiviral

Tags

mCherry-SspB

Expression

Mammalian

Mutation

Contains only amino acids 218-414

Promoter

SFFV

Available Since

April 1, 2019

Availability

Academic Institutions and Nonprofits only

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pRetroX-PTuner DD-M.EcoGII-v5-Centromeric protein C

Plasmid

#122085

Purpose

Express M.EcoGII fused to CENPC with destabilization domain - inducible

Depositor

Laure Crabbe

Article

Sobecki et al Cell Rep. 2018 Dec 4;25(10):2891-2903.e5

Insert

DD-linker-M.EcoGII-V5-CENPC (CENPC E.coli, Human)

Use

Retroviral

Tags

V5 tag on Nter of CENPC

Expression

Bacterial and Mammalian

Mutation

L341F, R930L (see comments below)

Available Since

March 15, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT/T0-Flag-RECQL5

Plasmid

#222620

Purpose

Mammalian expression vector of flag-tagged RECQL5

Depositor

Daniel Durocher

Article

Zhao et al Mol Cell. 2023 Aug 3;83(15):2792-2809.e9

Insert

RECQL5 (RECQL5 Human)

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available Since

July 25, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pET28a-MH6-Bsu

Plasmid

#163911

Purpose

Expresses Bsu large fragment for affinity purification

Depositor

Paul Freemont

Article

Patchsung et al CRISPR J. 2022 Nov 11. doi: 10.1089/cris

Insert

Bsu polymerase

Tags

His

Expression

Bacterial

Promoter

T7 promoter

Available Since

March 21, 2022

Availability

Academic Institutions and Nonprofits only

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pHR-HaloTag-P2A-RSV N-1xSunTag@105AA

Plasmid

#246868

Purpose

Express HaloTag-P2A-RSV N-1xSunTag@105AA (Nexo-SunTag) for RSV imaging.

Depositor

Marvin Tanenbaum

Article

Ratnayake et al Nature. 2026 Jan 28. doi: 10.1038/s41586

Insert

HaloTag-P2A-RSV N-1xSunTag@105AA

Use

Lentiviral

Tags

HaloTag

Expression

Mammalian

Mutation

The RSV nucleoprotein (N) with an insertion of a …

Promoter

SFFV

Available Since

Jan. 29, 2026

Availability

Academic Institutions and Nonprofits only

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LCV2_AAVS1_sgRNA_2

Plasmid

#155088

Purpose

lentiviral plasmid expressing Cas9 and gRNA targeting AAVS1 safe harbor locus

Depositor

Jason Moffat

Article

Aregger et al Nat Metab. 2020 Jun;2(6):499-513. doi: 1

Insert

AAVS1_sgRNA_2

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6 promoter

Available Since

Aug. 7, 2020

Availability

Academic Institutions and Nonprofits only

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M23: pHR-SFFVp-FUSN::mCherry::SspB

Plasmid

#122148

Purpose

N terminal domain of FUS (1-214) tagged with SspB and mCherry.

Depositor

Clifford Brangwynne

Article

Bracha et al Cell. 2018 Nov 29;175(6):1467-1480.e13.

Insert

FUS (FUS Human)

Use

Lentiviral

Tags

mCherry-sspB

Expression

Mammalian

Mutation

Contains only amino acids 1-214

Promoter

SFFV

Available Since

March 22, 2019

Availability

Academic Institutions and Nonprofits only

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pWZL-Neo-Myr-Flag-DEST

Plasmid

#15300

Purpose

a Gateway-compatible retroviral destination vector which adds a myristoylation sequence and a FLAG tag to each introduced ORF

Depositor

Jean Zhao

Article

Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

Retroviral

Tags

Flag and Myr

Expression

Mammalian

Available Since

July 12, 2007

Availability

Academic Institutions and Nonprofits only

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GFP-Myo10

Plasmid

#135403

Purpose

Expresses GFP-tagged bovine Myo10 in mammalian cells.

Depositor

Richard Cheney

Article

Berg et al Nat Cell Biol. 2002 Mar;4(3):246-50. doi

Insert

Myo10 (MYO10 Bovine)

Tags

EGFP

Expression

Mammalian

Mutation

"Relative to the GenBank Myo10 cDNA sequence…

Promoter

CMV

Available Since

March 30, 2022

Availability

Academic Institutions and Nonprofits only

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pET28a-MH6-uvsY

Plasmid

#163914

Purpose

Expresses uvsY for bacterial expression and affinity purification

Depositor

Paul Freemont

Article

Patchsung et al CRISPR J. 2022 Nov 11. doi: 10.1089/cris

Insert

uvsY (uvsY Enterobacteria phage T4)

Tags

His

Expression

Bacterial

Promoter

T7 promoter

Available Since

March 21, 2022

Availability

Academic Institutions and Nonprofits only

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pDONR223 SARS-CoV-2 NSP6

Plasmid

#141260

Purpose

Gateway-compatible Entry vector, with insert of NSP6 gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1

Depositor

Fritz Roth

Article

Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

Insert

NSP6 (ORF1ab SARS-CoV-2 isolate Wuhan-Hu-1)

Use

Gateway-compatible entry vector

Mutation

Many synonymous changes due to codon optimization

Available Since

April 1, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pDONR223-ZNF423

Plasmid

#88083

Purpose

Donor Vector containing ZNF423 transcription factor, part of the Human TFome Collection

Depositor

Marc Vidal

Article

Ng et al Nat Biotechnol. 2021 Apr;39(4):510-519.

Insert

ZNF423 (ZNF423 Human)

Use

Gateway donor vector

Mutation

Last nucleotide of stop codon removed to allow fo…

Available Since

June 3, 2021

Availability

Academic Institutions and Nonprofits only

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pLViN-E-cadherin-GFP

Plasmid

#229710

Purpose

Lentiviral expression of E-cadherin-GFPTG in mammalian cells

Depositor

Alpha Yap

Article

Noordstra et al Dev Cell. 2023 Sep 25;58(18):1748-1763.e

Insert

CDH1 E-CADHERIN Human (CDH1 Human)

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Promoter

CMV promoter

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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M30: pHR-SFFVp- HNRNPA1C::mCherry::SspB

Plasmid

#122668

Purpose

C terminal domain of hnRNPA1 (186-320) tagged with SspB and mCherry.

Depositor

Clifford Brangwynne

Article

Bracha et al Cell. 2018 Nov 29;175(6):1467-1480.e13.

Insert

HNRNPA1 (HNRNPA1 Human)

Use

Lentiviral

Tags

mCherry-SspB

Expression

Mammalian

Mutation

Contains only amino acids 186-230

Promoter

SFFV

Available Since

April 1, 2019

Availability

Academic Institutions and Nonprofits only

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pBFC0619

Plasmid

#186690

Purpose

VcDART under control of Lac promoter, Vc_2xBsaI_NT gRNA, GmR+sfGFP cargo

Depositor

Jennifer Doudna

Article

Rubin et al Nat Microbiol. 2022 Jan;7(1):34-47. doi:

Inserts

tnsA
tnsB
tnsC
tnsD
tniQ
cas8-cas5 fusion
cas7
cas6
Vc_2xBsaI_NT (Guide Stuffer) crRNA
Tn7 transposon
GmR+sfGFP VcDART Cargo

Expression

Bacterial

Promoter

PLac

Available Since

Sept. 26, 2022

Availability

Academic Institutions and Nonprofits only

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N-Terminal Split Cas9 with GyrA intein

Plasmid

#58693

Purpose

Expresses truncated N-terminal SpCas9 domain fused to a GyrA intein, flanked by ITRs for AAV packaging. Combine with C-Terminal Split Cas9 Gyra Intein for full length SpCas9 production

Depositor

Gang Bao

Article

Fine et al Sci Rep. 2015 Jul 1;5:10777. doi: 10.103

Insert

Humanized N-Terminal S. pyogenes Cas9 with GyrA Nsplit Intein

Use

AAV and CRISPR

Tags

3xFlag, GyrA Nsplit Intein, and NLS

Expression

Mammalian

Promoter

CBh

Available Since

July 6, 2015

Availability

Academic Institutions and Nonprofits only

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M43: pHR-SFFVp-iLID::EGFP::FTH1

Plasmid

#122149

Purpose

Self-assebled 24-mer tagged with iLID, and EGFP. Upon blue-light activation can bind up to 24 SspB-modified proteins.

Depositor

Clifford Brangwynne

Article

Bracha et al Cell. 2018 Nov 29;175(6):1467-1480.e13.

Insert

FTH1 (FTH1 Human)

Use

Lentiviral

Tags

NLS-iLID-GFP

Expression

Mammalian

Promoter

SFFV

Available Since

March 22, 2019

Availability

Academic Institutions and Nonprofits only

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