We narrowed to 15,686 results for: genome
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Plasmid#86618PurposeExpresses the ATP1A1 G5 crRNA in combination with AsCpf1-3xHA to target ATP1A1 intron 4. U6-crRNA(ATP1A1 G5)-CBh-AsCpf1DepositorInsertATP1A1 G5 crRNA + AsCpf1-3xHA
UseCRISPR; Co-selection via hdr using ouabainTags3xHAExpressionMammalianPromoterCBhAvailable SinceMarch 16, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLenti-161K enhancer-ETS-CORE-MUT-minP:fLuc-TK:rLuc
Plasmid#138367PurposeLentiviral plasmid contains 161k modified human enhancer with with ETS binding sites mutated driving firefly Luciferase 2 and Renilla Luciferase driven by TK promoterDepositorInsert161K human enhancer, with ETS binding sites mutated, driving firefly enhancer and TK driving refills luciferase
UseLentiviralExpressionMammalianAvailable SinceFeb. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pYPQ141-ZmUbi-RZ-Aa3.8.4
Plasmid#136375PurposeGateway entry clone for AaCas12b sgRNA expression under ZmUbi promoter with ribozyme processing; sgRNA scaffold 3.8 with MS2 at the third stem loop is usedDepositorTypeEmpty backboneUseCRISPRExpressionPlantAvailable SinceFeb. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
Zebrafish-gRNA-0001
Plasmid#42241PurposegRNA targeted to zebrafish gene apoeaDepositorAvailable SinceJan. 31, 2013AvailabilityAcademic Institutions and Nonprofits only -
lentiCRISPR - Amplicon, JAK2 sgRNA 1
Plasmid#70679PurposeExpresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and an intergenic JAK2 amplicon-targeting sgRNA element from U6 promoter. Lentiviral backboneDepositorInsertsgRNA against JAK2 amplicon found in AML-derived HEL cell line (JAK2 )
UseCRISPR and LentiviralExpressionMammalianAvailable SinceOct. 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMOD_C2519
Plasmid#91088PurposeModule C, Promoter: OsU3, Gene: Esp3I ccdb cassette for single gRNA spacer cloning (+ BaeI site for GT donor cloning), Terminator: Pol IIIDepositorInsertEsp3I ccdb cassette for single gRNA spacer cloning (+ BaeI site for GT donor cloning)
UseCRISPRPromoterOsU3Available SinceJuly 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSC2
Plasmid#91180PurposeT-DNA vector for combinatorial deletion of NCR genes in medicago truncatula (Csy4 array of 6 gRNAs under the control of CmYLCV promoter)DepositorInsertgRNAs targeting NCR genes
UseCRISPRExpressionPlantAvailable SinceSept. 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
pYPQ292 (AaCas12b)-D570A-TV
Plasmid#136380PurposeCRISPRa Gateway entry clone for catalytically dead AaCas12b (D570A) fused with TV activation systemDepositorInsertAaCas12b (D570A)-TV
UseCRISPRExpressionPlantMutationD570AAvailable SinceFeb. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
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LLP251 pEF1a-UNC5C-4-SunTag
Plasmid#100940PurposeTALE vector with UNC5C-4 insert fused to SunTagx10, driven by EF1a, with 3xHA tag, no PURO geneDepositorInsertTALE target sequence for UNC5C, SunTag array (10xGCN4)
UseTALENTags3xHAExpressionMammalianAvailable SinceJune 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
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pKLV2-U6gRNA5(Vps39-g1)-PGKpuroBFP-W
Plasmid#105019PurposeLentiviral gRNA plasmid targeting mouse Vps39 , co-expression of TagBFPDepositorAvailable SinceSept. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFETCh_ARID4B
Plasmid#101072PurposeDonor vector for 3' FLAG tag of human ARID4BDepositorAvailable SinceSept. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pQTEV-CUTC
Plasmid#34801DepositorInsertcutC copper transporter homolog (E. coli) (CUTC Human)
TagsHis and StrepExpressionBacterialAvailable SinceApril 9, 2013AvailabilityAcademic Institutions and Nonprofits only -
CNC3 (CASANOVA-C3)
Plasmid#137191PurposeExpresses CNC3 (CASANOVA-C3), a blue light dependent NmeCas9 inhibitory proteinDepositorInsertCNC3 (CASANOVA-C3)
UseCRISPR and Synthetic BiologyExpressionMammalianPromoterCMVAvailable SinceFeb. 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFETCh_GABPB1_iso2
Plasmid#104035PurposeDonor vector for 3' FLAG tag of human GABPB1_iso2DepositorInsertGABPB1_iso2 homology arms (GABPB1 Human)
UseCRISPRTags3XFLAG-P2A-NeoRExpressionMammalianAvailable SinceDec. 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLenti-X1-Neo-HA-UFL1-RAMP4
Plasmid#139863PurposeLentiviral expression of fusion of human UFL1 (full length) with RAMP4DepositorInsertUFL1-RAMP4 (UFL1 Human)
UseLentiviralTagsHAMutationrecombinant fusion of UFL1 (full length) with RAM…Available SinceMarch 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
pQTEV-EPSTI1
Plasmid#34826DepositorAvailable SinceMarch 22, 2013AvailabilityAcademic Institutions and Nonprofits only