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pSB1C3-hsg(M)

Plasmid

#138262

Purpose

Subcloning and expression of mCherry-targeting sgRNA M for use in Traffic Light reporter system

Depositor

Xiao Wang

Article

Standage-Beier et al CRISPR J. 2019 Aug;2:209-222. doi: 10.10

Insert

sgRNA M

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

March 3, 2020

Availability

Academic Institutions and Nonprofits only

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FmnI GST369/pGEX6p1

Plasmid

#19322

Depositor

Philip Leder

Article

Zhou et al Exp Cell Res. 2006 Apr 15. 312(7):1119-2

Insert

ForminI Exon 9 (Fmn1 Mouse)

Expression

Bacterial

Available Since

Sept. 15, 2008

Availability

Academic Institutions and Nonprofits only
pAG189 CASC2 3'UTR

Plasmid

#12017

Depositor

David Bartel

Article

Farh et al Science. 2005 Dec 16. 310(5755):1817-21.

Insert

N21 3'UTR (CASC2 Human)

Use

Luciferase

Expression

Mammalian

Available Since

June 2, 2006

Availability

Academic Institutions and Nonprofits only

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pSicoR-PTPRKi2

Plasmid

#59611

Purpose

Expression of shRNA against human PTPRK

Depositor

Miguel Ramalho-Santos

Article

Qin et al Cell. 2014 Jul 17;158(2):449-61. doi: 10

Insert

PTPRK (PTPRK Human)

Use

RNAi

Expression

Mammalian

Promoter

U6

Available Since

Sept. 29, 2014

Availability

Academic Institutions and Nonprofits only
pSicoR-PTPRKi1

Plasmid

#59610

Purpose

Expression of shRNA against human PTPRK

Depositor

Miguel Ramalho-Santos

Article

Qin et al Cell. 2014 Jul 17;158(2):449-61. doi: 10

Insert

PTPRK (PTPRK Human)

Use

RNAi

Expression

Mammalian

Promoter

U6

Available Since

Sept. 29, 2014

Availability

Academic Institutions and Nonprofits only
pMK33/intein-Cas9_S219-3XFLAG

Plasmid

#133782

Purpose

Expresses human codon-optimized intein-Cas9

Depositor

Norbert Perrimon

Article

Viswanatha et al Elife. 2018 Jul 27;7. pii: 36333. doi: 1

Insert

Human codon-optimized intein-Cas9

Expression

Insect

Available Since

Jan. 31, 2020

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmHPat

Plasmid

#25029

Depositor

Elisa Izaurralde

Article

Haas et al J Cell Biol. 2010 Apr 19. 189(2):289-302

Insert

HPat (Patr-1 Fly)

Use

Drosophila expression

Tags

EGFP

Available Since

June 21, 2010

Availability

Academic Institutions and Nonprofits only

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p5Ecldn5bE1

Plasmid

#90113

Purpose

5' Gateway entry clone containing endothelial specific enhancer sequence of cldn5b

Depositor

Nathan Lawson

Article

Quillien et al Cell Rep. 2017 Jul 18;20(3):709-720. doi

Insert

cldn5bE1 (cldn5b Zebrafish)

Expression

Bacterial

Available Since

June 29, 2017

Availability

Academic Institutions and Nonprofits only

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pMSCV-PIG-miR-26a-2

Plasmid

#64230

Purpose

Expresses miR-26a-2 in mammalian cells

Depositor

Joshua Mendell

Article

Chang et al Nat Genet. 2008 Jan;40(1):43-50. Epub 20

Insert

miR-26a-2 (MIR26A2 Human)

Use

Retroviral

Expression

Mammalian

Available Since

Nov. 13, 2015

Availability

Academic Institutions and Nonprofits only

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pRL1058

Plasmid

#70686

Purpose

Tn5-based plasmid for transposition in Anabaena sp. strain PCC 7120. Bears an oriV, so when excised from a chromosome or megaplasmid it can carry its neighboring sequences when returned to E. coli.

Depositor

Peter Wolk

Article

Wolk et al Proc Natl Acad Sci U S A. 1991 Jun 15;88

Insert

Transposon Tn5-1058

Available Since

Dec. 4, 2015

Availability

Academic Institutions and Nonprofits only

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pPI192

Plasmid

#113219

Purpose

generic knockin vector for C-terminal tagging with mCherry

Depositor

Rob Kay

Article

Paschke et al PLoS One. 2018 May 30;13(5):e0196809. do

Type

Empty backbone

Use

Generic knockin vector for c-terminal tagging wit…

Tags

mCherry

Available Since

Aug. 6, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA-FLAG-Nv-NF-kB-Cys

Plasmid

#27260

Depositor

Thomas Gilmore

Article

Sullivan et al PLoS One. 2009 . 4(10):e7311.

Insert

Nv-NF-kB

Tags

FLAG

Expression

Mammalian

Mutation

"Cys allele" = Cysteine at aa 67

Available Since

Feb. 1, 2011

Availability

Academic Institutions and Nonprofits only
pBluescript_VEGFA-TS3_SpCas9

Plasmid

#107328

Purpose

U6 driven SpCas9 sgRNA expression for VEGFA site 3

Depositor

Scot Wolfe

Article

Bolukbasi et al Nat Commun. 2018 Nov 19;9(1):4856. doi:

Insert

VEGFA-TS3 sgRNA

Use

CRISPR

Promoter

U6

Available Since

Nov. 15, 2018

Availability

Academic Institutions and Nonprofits only

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pBluescript_VEGFA-TS3_NmCas9

Plasmid

#107329

Purpose

U6 driven NmCas9 sgRNA expression for VEGFA site 3

Depositor

Scot Wolfe

Article

Bolukbasi et al Nat Commun. 2018 Nov 19;9(1):4856. doi:

Insert

VEGFA-TS3 sgRNA

Use

CRISPR

Promoter

U6

Available Since

Nov. 15, 2018

Availability

Academic Institutions and Nonprofits only

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pBS C-ret

Plasmid

#119900

Purpose

Mouse Ret In situ hybridization probe

Depositor

David Ginty, Wenqin Luo

Article

Luo et al Neuron. 2009 Dec 24;64(6):841-56. doi: 1

Insert

ret (Ret Mouse)

Use

In situ hybridization probe

Available Since

March 29, 2019

Availability

Academic Institutions and Nonprofits only

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pSG5-p85α-ΔiSH2-N

Plasmid

#39498

Depositor

Julian Downward

Article

Rodriguez-Viciana et al Cell. 1997 May 2;89(3):457-67.

Insert

p85 ΔiSH2-N (PIK3R1 Bovine)

Use

Eukaryotic

Mutation

ΔiSH2-N = deletion of AA478-513

Available Since

Aug. 30, 2012

Availability

Academic Institutions and Nonprofits only
pFGL1344

Plasmid

#116902

Purpose

Alp6:Bar:mCherry (SPB/MTOC marker)

Depositor

Naweed Naqvi

Article

Yadav et al MBio. 2019 Jul 30;10(4). pii: mBio.01581

Inserts

Alp6 (partial ORF)
Alp6 downstream region

Use

Fungal expression (in magnaporthe oryzae)

Available Since

Aug. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pSicoR-TTF1i2

Plasmid

#59621

Purpose

Expression of shRNA against human TTF1

Depositor

Miguel Ramalho-Santos

Article

Qin et al Cell. 2014 Jul 17;158(2):449-61. doi: 10

Insert

TTF1 (TTF1 Human)

Use

RNAi

Expression

Mammalian

Promoter

U6

Available Since

Sept. 29, 2014

Availability

Academic Institutions and Nonprofits only
RHEBL1

Plasmid

#55684

Purpose

expression of mouse RHEBL1

Depositor

Julian Downward

Article

Fritsch et al Cell. 2013 May 23;153(5):1050-63. doi: 1

Insert

RHEBL1 (Rhebl1 Mouse)

Expression

Bacterial

Promoter

lac

Available Since

Sept. 30, 2014

Availability

Academic Institutions and Nonprofits only
EC.RNe.TYB1.a1-400.wt

Plasmid

#17949

Depositor

David McKay

Article

Caruthers et al J Biol Chem. 2006 Sep 15. 281(37):27046-

Insert

Escherichia coli RNase E residues 1-400

Tags

intein-chitin binding domain

Expression

Bacterial

Mutation

residues 1-400

Available Since

May 29, 2008

Availability

Academic Institutions and Nonprofits only