We narrowed to 11,806 results for: nsf

1. pAAV-hSyn-mCherry.3xFLAG-WPRE

   Plasmid

   #127861

   Purpose

   pAAV plasmid expressing an mCherry.3xFLAG fusion protein under the hSyn promoter

   Depositor

   Florian Freudenberg

   Article

   Candemir et al Eur Neuropsychopharmacol. 2016 Apr;26(4)

   Insert

   mCherry
   

   Use

   AAV

   Tags

   3xFLAG

   Promoter

   hSyn

   Available Since

   Aug. 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMXS-IRES-Blast SHMT1

   Plasmid

   #106304

   Purpose

   Express SHMT1

   Depositor

   Richard Possemato

   Article

   Minton et al Mol Cell. 2018 Feb 15;69(4):610-621.e5.

   Insert

   serine hydroxymethyltransferase 1 (SHMT1 Human)
   

   Use

   Retroviral

   Expression

   Mammalian

   Available Since

   Dec. 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV-GFAP-JEDI-1P-WPRE

   Plasmid

   #202616

   Purpose

   Genetically encoded voltage indicator (GEVI) JEDI-1P in AAV production vector for astrocytes specific expression using the promoter GFAP

   Depositor

   Francois St-Pierre

   Article

   Lu et al Nat Commun. 2023 Oct 12;14(1):6423. doi:

   Insert

   JEDI-1P
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   GFAP

   Available Since

   June 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAcGP67A-rCL1-HormR-GAIN

   Plasmid

   #37847

   Depositor

   Axel Brunger

   Article

   Arac et al EMBO J. 2012 Feb 14;31(6):1364-78. doi:

   Insert

   CL1 (Adgrl1 Rat)
   

   Use

   Baculovirus transfer vectors

   Tags

   8xHIS

   Mutation

   HormR and GAIN domains only (aa460–849

   Promoter

   Polyhedrin

   Available Since

   July 23, 2012

   Availability

   Academic Institutions and Nonprofits only

5. pAAV2-SYN-GFP-BGHpA

   Plasmid

   #190229

   Purpose

   Expresses EGFP in neuronal cells

   Depositor

   Hiroyuki Hioki

   Article

   Sohn et al PLoS One. 2017 Jan 6;12(1):e0169611. doi

   Insert

   EGFP
   

   Use

   AAV

   Expression

   Mammalian

   Available Since

   Nov. 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV-syn-IRES-hrGFP

   Plasmid

   #107549

   Purpose

   high transduction efficiency AAV-mediated synapsin promoter-dependent expression IRES-hrGFP

   Depositor

   Hélène Marie

   Article

   Pousinha et al Elife. 2017 Jul 6;6. doi: 10.7554/eLife.

   Type

   Empty backbone

   Use

   AAV

   Available Since

   May 1, 2018

   Availability

   Academic Institutions and Nonprofits only

7. PE_CO-Mini-N

   Plasmid

   #190336

   Purpose

   Expressing the N-terminal of PE_CO-Mini using Rma intein and Cas9 673-674 split site, ngRNA and pegRNA for PCSK9 +1A insertion

   Depositor

   Rasmus Bak

   Article

   Gao et al Mol Ther. 2022 Jul 8. pii: S1525-0016(22

   Insert

   PE_CO-Mini-N, ngRNA and pegRNA for PCSK9 +1A insertion
   

   Use

   AAV

   Available Since

   Oct. 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV-SMVP-Cas9N-U6-gRNA TdL

   Plasmid

   #80938

   Purpose

   Expresses Cas9N in mammalian cells; expresses gRNA TdL for Ai9 cleavage.

   Depositor

   George Church

   Article

   Chew et al Nat Methods. 2016 Sep 5. doi: 10.1038/nm

   Insert

   Cas9N
   

   Use

   AAV, CRISPR, and Synthetic Biology

   Expression

   Mammalian

   Available Since

   Oct. 3, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV.GFAP.(cyto).iATPSnFR2.A95K.HaloTag

   Plasmid

   #209660

   Purpose

   Expression of iATPSnFR2, medium affinity

   Depositor

   Tim Brown, HHMI-JRC Tool Translation Team

   Article

   Marvin et al Proc Natl Acad Sci U S A. 2024 May 21;12

   Insert

   iATPSnFR2.A95K.HaloTag
   

   Use

   AAV

   Mutation

   A95K

   Promoter

   GFAP

   Available Since

   Nov. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV-Ef1a-DIO-hBFP-2A-RVG-WPRE

    Plasmid

    #120268

    Purpose

    Permits retrograde transsynaptic spread by driving Cre-dependent co-expression of the RbG plus a nuclear reporter

    Depositor

    Edward Callaway

    Article

    Faget et al Cell Rep. 2016 Jun 21;15(12):2796-808. d

    Insert

    EF1α-DIO-H2B-tagBFP-Flagx3-T2Am-cB19G
    

    Use

    AAV

    Expression

    Mammalian

    Available Since

    March 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pDGE652

    Plasmid

    #153230

    Purpose

    Recipient plant transformation vector containing selection markers and a Cas9 expression cassette, but lacking guide RNAs

    Depositor

    Sylvestre Marillonnet

    Article

    Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Plant

    Available Since

    Sept. 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. AAV-GfaABC1D-DreO-4x6T

    Plasmid

    #196413

    Purpose

    Astrocytic expression of Dre recombinase in AAV; astrocyte specificity enhanced with 4x6T miRNA targeting cassette

    Depositor

    Stanley Thomas Carmichael

    Article

    Gleichman et al Nat Commun. 2023 Nov 16;14(1):7426. doi:

    Insert

    DreO
    

    Use

    AAV; Astrocyte-selective

    Expression

    Mammalian

    Promoter

    CAG and GfaABC1D

    Available Since

    Feb. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV-hSyn-DIO {ChETA}on-WPRE

    Plasmid

    #111386

    Purpose

    AAV expression of ChETA (hChR2 with E123T/H134R mutations) fused to HA tag driven by hSynapsin promoter in Cre-ON manner for optogenetic activation

    Depositor

    Adam Kepecs

    Article

    Li et al Neuron. 2018 Jun 6;98(5):905-917.e5. doi

    Insert

    ChETA
    

    Use

    AAV

    Tags

    HA

    Expression

    Mammalian

    Mutation

    humanized ChR2, E123T/H134R

    Promoter

    hSynapsin

    Available Since

    June 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. -4200GAMT-P1-pGL4

    Plasmid

    #26804

    Depositor

    Sam Lee

    Article

    Ide et al Mol Cell. 2009 Nov 13. 36(3):379-92.

    Insert

    Guanidinoacetate N-methyltransferase (GAMT Human)
    

    Use

    Luciferase

    Available Since

    Jan. 6, 2011

    Availability

    Academic Institutions and Nonprofits only

15. eGFP Y93H GC editing reporter

    Plasmid

    #198884

    Purpose

    Express eGFP fluorescence after Y93H correction and mCherry as transfection control

    Depositor

    Reuben Harris

    Article

    Rieffer et al CRISPR J. 2023 Oct;6(5):430-446. doi: 10

    Insert

    enhanced green fluorescent protein
    

    Use

    Lentiviral

    Expression

    Mammalian

    Mutation

    Y93H, Gly92(GGT-to-GGG)

    Promoter

    CMV

    Available Since

    Nov. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pSBL_DK186

    Plasmid

    #156422

    Purpose

    Mammalian expression of 3a-EGFP with CMV promoter, can be used for transfection or used to generate bacmids for baculoviral infection of mammalian cells

    Depositor

    Stephen Brohawn

    Article

    Kern et al bioRxiv [Preprint]. 2021 Jan 26:2020.06.

    Insert

    SARS-CoV-2 3a protein
    

    Tags

    EGFP-His

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAV-TRE-DIO-eYFP-f

    Plasmid

    #104057

    Purpose

    An AAV genome with tet-inducible, Cre-dependent expression of the fluorescent protein eYFP with a C-terminal Hras farnesylation sequence

    Depositor

    Viviana Gradinaru

    Article

    Challis et al Nat Protoc. 2019 Feb;14(2):379-414. doi:

    Insert

    EYFP-f
    

    Use

    AAV

    Promoter

    TRE

    Available Since

    June 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pENN.AAV.CB7.CI.PM20D1-flag.WPRE.rBG

    Plasmid

    #132682

    Purpose

    AAV plasmid encoding mouse PM20D1 with C-terminal flag tag

    Depositor

    Jonathan Long

    Article

    Kim et al Cell Chem Biol. 2019 Nov 21;26(11):1623-

    Insert

    Peptidase M20 domain containing 1 (Pm20d1 Mouse)
    

    Use

    AAV

    Tags

    6xHIS and Flag

    Promoter

    Chicken b-actin

    Available Since

    Nov. 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pZac2.1 GfaABC1D ChR2(H134R) mCherry SV40

    Plasmid

    #112496

    Purpose

    Expresses channel rhodopsin in astrocytes

    Depositor

    Baljit Khakh

    Article

    Octeau et al Cell Rep. 2019 May 21;27(8):2249-2261.e7

    Insert

    ChR2(H134R)
    

    Use

    AAV

    Tags

    mCherry

    Mutation

    H134R

    Promoter

    GfaABC1D

    Available Since

    May 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAAV-CAG-miniSOG-T2A-dTomato

    Plasmid

    #193796

    Purpose

    Expresses miniSOG in the cytosol of mammalian cells, joined by a T2A cleavage site with dTomato

    Depositor

    Xiao Wang

    Article

    Sessler et al Sci Adv. 2022 Dec 9;8(49):eade1136. doi:

    Insert

    miniSOG
    

    Use

    AAV

    Tags

    Flag tag

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Jan. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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