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pLenti TurboRFP BlastR

Plasmid

#102343

Purpose

Lentiviral expression of cytoplasmic TurboRFP with Blasticidin selection in cells including neurons

Depositor

Ghassan Mouneimne

Article

Parker et al eNeuro. 2018 Sep 27;5(5). pii: eN-MNT-01

Insert

TurboRFP

Use

Lentiviral

Tags

N/A

Promoter

CMV immediate early

Available Since

May 22, 2018

Availability

Academic Institutions and Nonprofits only

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pAJM.847

Plasmid

#108524

Purpose

PhlFAM + PPhlF-YFP Reporter. DAPG sensor for use in non-Marionette strain.

Depositor

Christopher Voigt

Article

Meyer et al Nat Chem Biol. 2018 Nov 26. pii: 10.1038

Insert

PhlFAM + PPhlF-YFP

Expression

Bacterial

Mutation

PhlF: S50G S73I R95H A121V M137I N144D T155I R200C

Available Since

Aug. 14, 2018

Availability

Academic Institutions and Nonprofits only

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pET28-2H12.D11

Plasmid

#221145

Purpose

His6-tagged cdGreen2 expressed from T7 promoter (Internal Lab ID: UJ11357)

Depositor

Urs Jenal

Article

Kaczmarczyk et al Nat Commun. 2024 May 9;15(1):3920. doi:

Insert

cdGreen2

Expression

Bacterial

Promoter

T7

Available Since

June 25, 2024

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1-FLAG-Ku70

Plasmid

#46957

Purpose

Plasmid for cloning and epression of GFP-FLAG tagged human Ku70 (N-terminal tag). Confers resistance to G418.

Depositor

Steve Jackson

Article

Britton et al J Cell Biol. 2013 Jul 29.

Insert

Ku70 (XRCC6 Human)

Tags

GFP-FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 20, 2013

Availability

Academic Institutions and Nonprofits only

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pYPQ132-tRNA2.0

Plasmid

#158394

Purpose

Golden Gate entry vector to express the 2nd gRNA with tRNA2.0 scaffold (with four MS2 binding sites) without promoter

Depositor

Yiping Qi

Article

Pan et al Nat Plants. 2021 Jul;7(7):942-953. doi:

Type

Empty backbone

Use

CRISPR

Expression

Plant

Promoter

Without promoter

Available Since

June 25, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1/Puro-CAG-ASAP1

Plasmid

#52519

Purpose

For mammalian expression of the fluorescent voltage sensor ASAP1

Depositor

Michael Lin

Article

St-Pierre et al Nat Neurosci. 2014 Apr 22. doi: 10.1038/

Insert

Accelerated Sensor of Action Potentials 1

Expression

Mammalian

Promoter

CAG

Available Since

April 22, 2014

Availability

Academic Institutions and Nonprofits only

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pYPQ133-tRNA2.0

Plasmid

#158395

Purpose

Golden Gate entry vector to express the 3rd gRNA with tRNA2.0 scaffold (with four MS2 binding sites) without promoter

Depositor

Yiping Qi

Article

Pan et al Nat Plants. 2021 Jul;7(7):942-953. doi:

Type

Empty backbone

Use

CRISPR

Expression

Plant

Promoter

Without promoter

Available Since

June 25, 2021

Availability

Academic Institutions and Nonprofits only

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pYPQ134-tRNA2.0

Plasmid

#158396

Purpose

Golden Gate entry vector to express the 4th gRNA with tRNA2.0 scaffold (with four MS2 binding sites) without promoter

Depositor

Yiping Qi

Article

Pan et al Nat Plants. 2021 Jul;7(7):942-953. doi:

Type

Empty backbone

Use

CRISPR

Expression

Plant

Promoter

Without promoter

Available Since

June 25, 2021

Availability

Academic Institutions and Nonprofits only

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pYPQ135-tRNA2.0

Plasmid

#158397

Purpose

Golden Gate entry vector to express the 5th gRNA with tRNA2.0 scaffold (with four MS2 binding sites) without promoter

Depositor

Yiping Qi

Article

Pan et al Nat Plants. 2021 Jul;7(7):942-953. doi:

Type

Empty backbone

Use

CRISPR

Expression

Plant

Promoter

Without promoter

Available Since

June 25, 2021

Availability

Academic Institutions and Nonprofits only

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pYPQ136-tRNA2.0

Plasmid

#158398

Purpose

Golden Gate entry vector to express the 6th gRNA with tRNA2.0 scaffold (with four MS2 binding sites) without promoter

Depositor

Yiping Qi

Article

Pan et al Nat Plants. 2021 Jul;7(7):942-953. doi:

Type

Empty backbone

Use

CRISPR

Expression

Plant

Promoter

Without promoter

Available Since

June 25, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV.CAG.(cyto).cpSFGFP.HaloTag

Plasmid

#209654

Purpose

Non-responsive control

Depositor

Tim Brown, HHMI-JRC Tool Translation Team

Article

Marvin et al Proc Natl Acad Sci U S A. 2024 May 21;12

Insert

cpSFGFP.HaloTag

Use

AAV

Promoter

CAG

Available Since

Nov. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pYPQ-dpcoCas9-EV2.1

Plasmid

#158411

Purpose

CRISPR-dpcoCas9 fused with EDLL activator for transcriptional activation

Depositor

Yiping Qi

Article

Pan et al Nat Plants. 2021 Jul;7(7):942-953. doi:

Insert

dpcoCas9-EDLL-T2A-MS2-VPR(VP64-p65-Rta)-NOS-ter

Use

CRISPR

Expression

Plant

Available Since

June 25, 2021

Availability

Academic Institutions and Nonprofits only

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pMK345 (Hygro-P2A-mAID-mClover)

Plasmid

#121179

Purpose

N-terminal tagging

Depositor

Masato Kanemaki

Article

Yesbolatova et al Methods. 2019 Apr 23. pii: S1046-2023(18

Insert

Hygro-P2A-mAID-mClover

Use

CRISPR

Available Since

Feb. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pKLBac-FluB-trimer

Plasmid

#229859

Purpose

Influenza B/Memphis/13/03 polymerase heterotrimer (self-cleaving polyprotein)

Depositor

Stephen Cusack

Article

Reich et al Nature. 2014 Dec 18;516(7531):361-6. doi

Inserts

PA, Influenza B (B/Memphis/13/03)
PB1, Influenza B (B/Memphis/13/03)
PB2, Influenza B (B/Los Angeles/1/02)

Tags

2x-Streptag and 8xHis-tag

Expression

Insect

Available Since

Feb. 25, 2025

Availability

Academic Institutions and Nonprofits only

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wtTDP43tdTOMATOHA

Plasmid

#28205

Depositor

Zuoshang Xu

Article

Yang et al PLoS One. 2010 . 5(12):e15878.

Insert

Tar DNA binding protein (TARDBP Human)

Tags

HA tag and tdtomato

Expression

Mammalian

Available Since

June 1, 2011

Availability

Academic Institutions and Nonprofits only

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pBECAb-apr

Plasmid

#122001

Purpose

A cytidine deaminase-mediated base-editing plasmid in Acinetobacter baumannii

Depositor

Quanjiang Ji

Article

Wang et al Cell Chem Biol. 2019 Sep 17. pii: S2451-

Type

Empty backbone

Use

CRISPR

Available Since

Sept. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pNCS-mClover3

Plasmid

#74236

Purpose

Constitutive expression of mClover3 in bacteria

Depositor

Michael Lin

Article

Bajar et al Sci Rep. 2016 Feb 16;6:20889. doi: 10.10

Has Service

Cloning Grade DNA

Insert

mClover3

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

March 29, 2016

Availability

Academic Institutions and Nonprofits only

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pKIR1.1

Plasmid

#85758

Purpose

CRISPR/Cas9 in Arabidopsis with seed a fluorescent reporter

Depositor

Tetsuya Higashiyama

Article

Tsutsui et al Plant Cell Physiol. 2016 Nov 17. pii: pc

Insert

human-codon-optimized SpCas9

Use

CRISPR

Tags

FLAG

Expression

Plant

Promoter

AtRPS5A

Available Since

Jan. 10, 2017

Availability

Academic Institutions and Nonprofits only

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CMV-mito-Citroff1-S245

Plasmid

#134306

Purpose

mitochondria-targeted inverse-response citrate sensor variant with medium binding affinity

Depositor

Robert Campbell

Article

Zhao et al ACS Cent Sci. 2020 Aug 26;6(8):1441-1450

Insert

Citroff1 S245A

Expression

Mammalian

Promoter

CMV

Available Since

April 13, 2020

Availability

Academic Institutions and Nonprofits only

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pLM-fSV2A

Plasmid

#27512

Depositor

Michel Sadelain

Article

Papapetrou et al Nat Biotechnol. 2011 Jan;29(1):73-8. doi

Insert

OCT4_T2A_KLF4_P2A_cMYC_E2A_Sox2

Use

Lentiviral

Available Since

Feb. 25, 2011

Availability

Academic Institutions and Nonprofits only

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