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pCMV5 MYC-Smurf2 (FF29/30A) WT

Plasmid

#24604

Depositor

Jeff Wrana

Article

Ogunjimi et al J Biol Chem. 2010 Feb 26. 285(9):6308-15

Insert

Smurf2 (SMURF2 Human)

Tags

MYC

Expression

Mammalian

Mutation

F29A, F30A

Available Since

April 30, 2010

Availability

Industry, Academic Institutions, and Nonprofits

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3269 pBS Bcl-2

Plasmid

#8764

Depositor

Stanley Korsmeyer

Article

Silverman et al Proc Natl Acad Sci U S A 1990 Dec;87(24)

Insert

Bcl-2 (Bcl2 Mouse)

Expression

Bacterial

Available Since

July 6, 2005

Availability

Academic Institutions and Nonprofits only
pLR5-CAG-hMCP-mScarlet-I-NLS

Plasmid

#174878

Purpose

PiggyBac transposon vector for expression of MCP-mScarlet-I-NLS

Depositor

Hiroshi Ochiai

Article

Ohishi et al Nat Commun. 2022 Dec 20;13(1):7672. doi:

Insert

MCP-mScarlet-I-NLS

Use

Piggybac vector

Expression

Mammalian

Available Since

Dec. 22, 2022

Availability

Academic Institutions and Nonprofits only

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pMXs-puro-hMGT

Plasmid

#172393

Purpose

Retroviral expression vector for human direct cardiac reprogramming

Depositor

Li Qian

Article

Zhou et al Cell Stem Cell. 2019 Jul 3;25(1):149-164

Insert

Mef2c, Gata4, Tbx5 (MEF2C, GATA4, TBX5 Human)

Use

Retroviral

Available Since

July 16, 2021

Availability

Academic Institutions and Nonprofits only

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AY22_pgRNA.R5.1

Plasmid

#100294

Purpose

CCR5-targeting gRNA expression plasmid

Depositor

Manuel Goncalves

Article

Chen et al Nat Commun. 2017 Sep 22;8(1):657. doi: 1

Insert

gRNA spacer

Use

CRISPR and Synthetic Biology

Expression

Mammalian

Promoter

U6 RNA Pol III promoter human snRNA

Available Since

Sept. 22, 2017

Availability

Academic Institutions and Nonprofits only

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pSF4 CMV intron renilla TRICK CTE polyA

Plasmid

#84443

Purpose

TRICK reporter mRNA

Depositor

Jeffrey Chao

Article

Halstead et al Science. 2015 Mar 20;347(6228):1367-671.

Insert

Renill-TRICK

Use

Luciferase; Frt

Expression

Mammalian

Promoter

Tet-CMV

Available Since

Nov. 29, 2016

Availability

Academic Institutions and Nonprofits only

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pEGFP-WRAP53beta

Plasmid

#64676

Purpose

expresses Wild type WRAP53beta

Depositor

Marianne Farnebo

Article

Henriksson et al Genes Dev. 2014 Dec 15;28(24):2726-38. d

Insert

WD40-encoding RNA antisense to P53 (WRAP53 Human)

Tags

GFP

Expression

Mammalian

Promoter

cmv

Available Since

May 22, 2015

Availability

Academic Institutions and Nonprofits only
SpdCas9 KRAB

Plasmid

#188499

Purpose

Expresses FLAG tagged SpdCas9 KRAB

Depositor

Isaac Hilton

Article

Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

Insert

dCas9

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

D10A/H840A

Promoter

EF1a

Available Since

Nov. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pmCherry-HP1alpha

Plasmid

#179703

Purpose

visualization of centromere heterochromatin

Depositor

Kenji Sugimoto

Article

Fukada et al Biosci Biotechnol Biochem. 2019 Jul;83(7

Insert

heterochromatin protein alpha (CBX5 Human)

Tags

mCherry

Expression

Mammalian

Available Since

March 10, 2022

Availability

Academic Institutions and Nonprofits only

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pLR5-CBh-dCas9-hUtx-IRES-Hyg

Plasmid

#122374

Purpose

Expresses dCas9-hUtx-IRES-Hyg in mammalian cells

Depositor

Hiroshi Ochiai

Article

Ochiai et al Sci Adv. 2020 Jun 17;6(25):eaaz6699. doi

Insert

CBh-dCas9-hUtx-IRES-Hyg-pA

Use

Piggybac

Expression

Mammalian

Available Since

June 30, 2020

Availability

Academic Institutions and Nonprofits only

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Str-KDEL_SBP-EGFP-WNT3A

Plasmid

#108344

Purpose

synchronize trafficking of WNT3A from the ER (RUSH System)

Depositor

David Virshup

Article

Moti et al PLoS One. 2019 Feb 22;14(2):e0212711. do

Insert

WNT3A, Wnt family member 3A (WNT3A Human)

Tags

IL-2 Signal Sequence and Streptavidin Binding Pro…

Expression

Mammalian

Promoter

CMV

Available Since

March 26, 2019

Availability

Academic Institutions and Nonprofits only

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GW1-mito-roGFP1-mApple

Plasmid

#107218

Purpose

Mammalian expression plasmid to target the roGFP1-mApple sensor to the mitochondrial matrix.

Depositor

Mathew Tantama

Article

Norcross et al ACS Sens. 2017 Nov 22;2(11):1721-1729. d

Insert

roGFP1-mApple

Tags

4xCox8

Expression

Mammalian

Promoter

CMV

Available Since

March 15, 2018

Availability

Academic Institutions and Nonprofits only

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pUCmini-iCAP-PHP.B2

Plasmid

#103003

Purpose

non-standard AAV2 rep-AAV-PHP.B2 cap plasmid with AAV cap expression controlled by a tTA-TRE amplification system

Depositor

Viviana Gradinaru

Article

Deverman et al Nat Biotechnol. 2016 Feb;34(2):204-9. do

Insert

Synthetic construct isolate AAV-PHP.B2 VP1 gene

Use

AAV

Expression

Mammalian

Promoter

p41

Available Since

Dec. 15, 2017

Availability

Academic Institutions and Nonprofits only

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pCDH_SBP-mCherry-Ecadherin

Plasmid

#65291

Purpose

Ecadherin reporter only (no hook) (RUSH system)

Depositor

Franck Perez

Article

Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

Insert

Ecadherin

Use

Lentiviral

Tags

IL-2 Signal Sequence and Streptavidin Binding Pro…

Promoter

CMV

Available Since

Aug. 3, 2015

Availability

Academic Institutions and Nonprofits only

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Oct4-Cr1

Plasmid

#66989

Purpose

Expresses gRNA that targets stop codon for OCT4

Depositor

Danwei Huangfu

Article

Zhu et al Stem Cell Reports. 2015 May 28. pii: S22

Insert

gRNA for Crispr targeting the OCT4 stop codon

Expression

Mammalian

Available Since

July 22, 2015

Availability

Academic Institutions and Nonprofits only
pFPL-Gg

Plasmid

#61653

Purpose

Gateway vector for generating green fluorescent protein fusions in fungi

Depositor

Mark Farman

Article

Gong et al Mol Plant Microbe Interact. 2014 Nov 12.

Insert

enhanced green fluorescent protein

Expression

Plant

Available Since

May 26, 2015

Availability

Academic Institutions and Nonprofits only

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GFP-BRPF3

Plasmid

#65383

Purpose

GFP-tagged BRD protein, which can be used to be expressed in mammalian cells.

Depositor

Kyle Miller

Article

Gong et al Genes Dev. 2015 Jan 15;29(2):197-211. do

Insert

BRPF3 (BRPF3 Human)

Tags

EmGFP and V5

Expression

Mammalian

Mutation

none

Promoter

CMV

Available Since

May 14, 2015

Availability

Academic Institutions and Nonprofits only
pGLTR-X-PURO

Plasmid

#58246

Purpose

GATEWAY-compatible lentiviral conditional RNAi delivery vector; SFFV-driven expression of TetR and Puromycin selection

Depositor

Stephan Geley

Article

Sigl et al PLoS One. 2014 May 19;9(5):e97764. doi:

Insert

Puro(R)

Use

Lentiviral and RNAi; Gateway destionation vector

Tags

TetR-NLS-T2A

Expression

Mammalian

Promoter

SFFV

Available Since

Nov. 7, 2014

Availability

Academic Institutions and Nonprofits only

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GFP-Rnd2

Plasmid

#23228

Depositor

Channing Der

Article

Roberts et al J Biol Chem. 2008 Sep 12. 283(37):25150-

Insert

Rnd2 (RND2 Human)

Tags

EGFP

Expression

Mammalian

Available Since

March 24, 2010

Availability

Academic Institutions and Nonprofits only
D3 clone (p21/WAF1 promoter)

Plasmid

#16452

Depositor

Bert Vogelstein

Article

el-Deiry et al Cell. 1993 Nov 19. 75(4):817-25.

Insert

WAF1 promoter (CDKN1A Human)

Expression

Mammalian

Available Since

April 7, 2008

Availability

Academic Institutions and Nonprofits only