We narrowed to 22,767 results for: his

Showing: 11641 - 11670 of 22767 results

Results per page:

Sort results by:

pFastBac HTB DPP9S D808N

Plasmid

#169947

Purpose

Purification of catalytically-dead DPP9S from insect cells

Depositor

Hao Wu

Article

Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

Insert

DPP9 (DPP9 Human)

Tags

HIS-TEV

Expression

Insect

Mutation

D808N

Promoter

polyhedrin

Available Since

May 11, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
Brg1 Del4 pET28C

Plasmid

#122118

Purpose

Expression Brg1 amino acids 1446-1647 in bacteria

Depositor

Paul Herring

Article

Zhou et al Arterioscler Thromb Vasc Biol. 2009 Jun;

Insert

Brg1 (SMARCA4 Human)

Tags

His-T7

Expression

Bacterial

Available Since

March 15, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
IL2 F42A pAcGP67a

Plasmid

#41807

Depositor

Chris Garcia

Article

Levin et al Nature. 2012 Mar 25;484(7395):529-33. do

Insert

IL2 (IL2 Human)

Tags

6x His

Expression

Insect

Mutation

Phenylalanine 42 to Alanine

Promoter

Polyhedron

Available Since

Jan. 3, 2013

Availability

Academic Institutions and Nonprofits only
pcDNA3.1-mNRDc-E>A-V5

Plasmid

#51298

Purpose

expression of the inactive mutant of mouse NRDc in mammalian cells

Depositor

Eiichiro Nishi

Article

Hiraoka et al Nat Commun. 2014;5:3224. doi: 10.1038/nc

Insert

NRDc E247A (Nrd1 Mouse)

Tags

HIS and V5

Expression

Mammalian

Mutation

E247A, enzymatic inactive mutant

Promoter

CMV

Available Since

Feb. 14, 2014

Availability

Academic Institutions and Nonprofits only
pSBL_DK202

Plasmid

#156426

Purpose

Mammalian expression of 3a delta N(aa 42-275)-EGFP with CMV promoter, can be used for transfection or used to generate bacmids for baculoviral infection of mammalian cells

Depositor

Stephen Brohawn

Article

Kern et al bioRxiv [Preprint]. 2021 Jan 26:2020.06.

Insert

SARS-CoV-2 3a protein (aa 42-275)

Tags

EGFP-His

Expression

Mammalian

Mutation

aa 42-275

Promoter

CMV

Available Since

July 21, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
Brg1 Del1 pET28a

Plasmid

#122115

Purpose

Expression Brg1 amino acids 1-381 in bacteria

Depositor

Paul Herring

Article

Zhou et al Arterioscler Thromb Vasc Biol. 2009 Jun;

Insert

Brg1 (SMARCA4 Human)

Tags

His-T7

Expression

Bacterial

Available Since

Feb. 21, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
Sumo3 PolQM1 deltains1

Plasmid

#78461

Purpose

Inducible expression in E. coli

Depositor

Sylvie Doublie, Susan Wallace

Article

Hogg et al J Mol Biol. 2011 Jan 21;405(3):642-52. d

Insert

Polymerase theta (POLQ Human)

Tags

His and SUMO3

Expression

Bacterial

Mutation

delta 1-1791; delta 2149-2170; Q2513R (please see…

Promoter

T7

Available Since

July 28, 2016

Availability

Academic Institutions and Nonprofits only
pEBTet-SNAP-Cep76

Plasmid

#136813

Purpose

Mammalian expression of the centrosomal protein Cep76 N-terminally fused to SNAP-tag

Depositor

Kai Johnsson

Article

Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

Insert

SNAP-Cep76 (CEP76 Human, Synthetic)

Tags

FLAG-tag / His-tag

Expression

Mammalian

Promoter

CMV-TetO2

Available Since

March 26, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
CaV1.3e[8a,11,31b,Δ32,Δ42a] mut

Plasmid

#26577

Depositor

Diane Lipscombe

Article

Xu et al J Neurosci. 2001 Aug 15. 21(16):5944-51.

Insert

CACNA1D (Cacna1d Rat)

Expression

Mammalian

Mutation

Contains exons 8a, 11, and 31b.Exons 32 and 42a a…

Available Since

Jan. 31, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28a-PKM2-G415R

Plasmid

#122693

Purpose

Express His-tagged PKM2 (G415R) protein in bacteria.

Depositor

Wen-Ching Wang

Article

Chen et al Commun Biol. 2019 Mar 15;2:105. doi: 10.

Insert

Pyruvate kinase M2 isoform (PKM Human)

Tags

6His-tag

Expression

Bacterial

Mutation

G415R

Available Since

March 21, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pet23a_RBPMS_FL

Plasmid

#59392

Purpose

Plasmid for bacterial overexpression of RBPMS

Depositor

Thomas Tuschl

Article

Farazi et al RNA. 2014 Jul;20(7):1090-102. doi: 10.12

Insert

RBPMS (RBPMS Human)

Tags

His Tag

Expression

Bacterial

Promoter

T7

Available Since

Feb. 9, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pEBTet-SNAP-Cep41

Plasmid

#136794

Purpose

Mammalian expression of the centrosomal protein Cep41 N-terminally fused to SNAP-tag

Depositor

Kai Johnsson

Article

Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

Insert

SNAP-Cep41 (CEP41 Human, Synthetic)

Tags

FLAG-tag / His-tag

Expression

Mammalian

Promoter

CMV-TetO2

Available Since

March 25, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pEBTet-SNAP-α-tubulin

Plasmid

#136791

Purpose

Mammalian expression of the centrosomal protein _-tubulin N-terminally fused to SNAP-tag

Depositor

Kai Johnsson

Article

Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

Insert

SNAP-TUBA (DNMBP Human, Synthetic)

Tags

FLAG-tag / His-tag

Expression

Mammalian

Promoter

CMV-TetO2

Available Since

March 25, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
p53-(1-393)-E285K-N268D

Plasmid

#35089

Depositor

Cheryl Arrowsmith

Article

Sheng et al Nat Struct Mol Biol. 2008 Dec . 15(12):1

Insert

p53 (TP53 Human)

Tags

His and Thrombin cleavage site

Expression

Bacterial

Mutation

Contains aa 1-393 with N269D and E285K mutations

Promoter

T7

Available Since

April 4, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
Dom34(full length)

Plasmid

#19098

Depositor

SeWon Suh

Article

Lee et al Mol Cell. 2007 Sep 21. 27(6):938-50.

Insert

Protein DOM34 (DOM34 Budding Yeast)

Tags

His

Expression

Bacterial

Available Since

June 22, 2010

Availability

Academic Institutions and Nonprofits only
Sumo3 PolQM1 deltains2

Plasmid

#78460

Purpose

Inducible expression in E. coli

Depositor

Sylvie Doublie, Susan Wallace

Article

Hogg et al J Mol Biol. 2011 Jan 21;405(3):642-52. d

Insert

Polymerase theta (POLQ Human)

Tags

His and SUMO3

Expression

Bacterial

Mutation

delta 1-1791; delta 2264-2315; Q2513R (see deposi…

Available Since

July 28, 2016

Availability

Academic Institutions and Nonprofits only
hHSF1 S303A

Plasmid

#118363

Purpose

This plasmid expresses human HSF1 S303A mutant, which cannot undergo sumoylation on K298 due to disrupted PDSM motif.

Depositor

Lea Sistonen

Article

Hietakangas et al Mol Cell Biol. 2003 Apr . 23(8):2953-68.

Insert

hHSF1 (HSF1 Human)

Tags

myc and 6xHIS

Expression

Mammalian

Mutation

S303A

Available Since

Nov. 8, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pJSC282 - Bacterial expression plasmid for SpCas9 Cluster 1 (HypaCas9), REC3 FRET variant

Plasmid

#101231

Purpose

Bacterial expression plasmid for SpCas9 Cluster 1 (HypaCas9), REC3 FRET variant

Depositor

Jennifer Doudna, Keith Joung

Article

Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

Insert

SpCas9 variant C80S/C574S/S701C/S960C/N692A/M694A/Q695A/H698A

Tags

10x His, MBP, and TEV site

Expression

Bacterial

Mutation

C80S, C574S, S701C, S960C, N692A, M694A, Q695A an…

Promoter

T7

Available Since

Nov. 6, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
SPRR2A-pFastBac I

Plasmid

#182460

Purpose

Expression His-tagged SPRR2A in insect cells

Depositor

Lora Hooper

Article

Hu et al Science. 2021 Nov 5;374(6568):eabe6723.

Insert

small proline rich protein 2A (SPRR2A Human)

Tags

6xHis

Expression

Insect

Promoter

Polyhedrin

Available Since

March 3, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
LV_EF1a_Stat3(A662C,N664C,V667L)-P2A-Hygro_Barcode

Plasmid

#170254

Purpose

Barcoded lentiviral vector to express Stat3 (A662C, N664C, V667L) in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq

Depositor

Prashant Mali

Article

Parekh et al iScience. 2021 Sep 20;24(10):103149. doi

Insert

STAT3 (STAT3 Human)

Use

Lentiviral

Mutation

A662C, N664C, V667L

Promoter

EF1a

Available Since

Sept. 23, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
MBP-hnRNPA2_FL_P298L

Plasmid

#104468

Purpose

express MBP-tagged full length hnRNPA2 P298L

Depositor

Nicolas Fawzi

Article

Ryan et al Mol Cell. 2018 Jan 17. pii: S1097-2765(1

Insert

hnRNPA2 (HNRNPA2B1 Human)

Tags

His-MBP

Expression

Bacterial

Mutation

P298L

Available Since

Jan. 18, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pET20b-delta133HtrA2 A141S

Plasmid

#16157

Depositor

L. Miguel Martins

Article

Strauss et al Hum Mol Genet. 2005 Aug 1. 14(15):2099-1

Insert

HtrA2 (HTRA2 Human)

Tags

His

Expression

Bacterial

Mutation

A141S

Available Since

Feb. 1, 2008

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA Flag-RARalpha (T181A,S445A,S461A)

Plasmid

#35556

Depositor

Jonathan Kurie, Harish Srinivas

Article

Srinivas et al Mol Cell Biol. 2005 Feb;25(3):1054-69.

Insert

RARalpha (RARA Human)

Tags

Flag and His

Expression

Mammalian

Mutation

T181A, S445A, S461A

Available Since

April 5, 2012

Availability

Academic Institutions and Nonprofits only
pASK/3xMBT

Plasmid

#28234

Depositor

Danny Reinberg

Article

Trojer et al Cell. 2007 Jun 1. 129(5):915-28.

Insert

l(3)mbt-like 1 (Drosophila) (L3MBTL1 Human)

Tags

His

Expression

Bacterial

Mutation

Truncation expressing only aa 252-606 (MBT repeat…

Available Since

May 23, 2011

Availability

Academic Institutions and Nonprofits only
HOXB4 (human) HA-TAT-tag pET

Plasmid

#8526

Depositor

Corey Largman

Article

Shen et al Proc Natl Acad Sci U S A 1989 Nov;86(21)

Insert

HOXB4 (HOXB4 Human)

Tags

HA

Expression

Bacterial

Mutation

replace T7 and HIS tags of pET with an HA tag;

Available Since

May 30, 2006

Availability

Academic Institutions and Nonprofits only
pET41a-MED18

Plasmid

#15433

Depositor

Joan Conaway, Ronald Conaway

Article

Sato et al J Biol Chem. 2003 Apr 25. 278(17):15123-

Insert

MED18 (MED18 Human)

Tags

GST and His

Expression

Bacterial

Mutation

N/A

Available Since

Aug. 10, 2007

Availability

Academic Institutions and Nonprofits only
pEBTetDBl-CLIP-α-tubulin

Plasmid

#136841

Purpose

Mammalian expression of the centrosomal protein _-tubulin N-terminally fused to CLIP-tag

Depositor

Kai Johnsson

Article

Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

Insert

CLIP-TUBA (DNMBP Human, Synthetic)

Tags

FLAG-tag / His-tag

Expression

Mammalian

Promoter

CMV-TetO2

Available Since

March 31, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pEBTet-SNAP-Cep70

Plasmid

#136821

Purpose

Mammalian expression of the centrosomal protein Cep70 N-terminally fused to SNAP-tag

Depositor

Kai Johnsson

Article

Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

Insert

SNAP-Cep70 (CEP70 Human, Synthetic)

Tags

FLAG-tag / His-tag

Expression

Mammalian

Promoter

CMV-TetO2

Available Since

March 26, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
human TLNRD1-F250D pET151

Plasmid

#159385

Purpose

Expresses human TLNRD1 F250D mutant in bacterial cells

Depositor

Ben Goult

Article

Cowell et al J Cell Biol. 2021 Sep 6;220(9). pii: 212

Insert

Talin Rod Domain Containing Protein 1 F250D mutant (TLNRD1 Human)

Tags

His-tag, TEV cleavage site

Expression

Bacterial

Mutation

Changed Phenylalanine 250 to Aspartic Acid (F250D)

Promoter

T7

Available Since

July 19, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pIE-C-hα2M-H6

Plasmid

#128494

Purpose

Expresses human C-α2M (from R787 to N1473) in insect cells. With C-t H6x tag.

Depositor

F Xavier Gomis-Ruth

Article

Marino-Puertas et al Sci Rep. 2019 Jun 24;9(1):9186. doi: 10.

Insert

A2M (R787-N1473) (A2M Human)

Tags

His tag (6x)

Expression

Insect

Mutation

contains R787-N1473

Promoter

IE1

Available Since

Aug. 15, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge