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Showing: 1141 - 1170 of 17336 results

  3. pUC19-HDRT-TRAC-CD19.CAR-Cas12a.PAM.mutated

    Plasmid
    #215769
    Purpose
    encodes for HDR template for optimized AsCas12a-mediated knock-in of a CD19-specific CAR into the TRAC locus
    Depositor
    Dimitrios Wagner
    Article
    Glaser et al Genome Biol. 2023 Apr 24;24(1):89. doi:
    Inserts
    TRAC-5' homology arm (TRAC Human)
    P2A-CD19.28.zeta-bgH (CD19 Bovine, Mouse, Human)
    TRAC-3' homology arm (TRAC Human)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Cas12a PAM mutated
    Promoter
    Available since
    June 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    215769-plasmid-map-sequence-id-435524

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  5. pCDNA3-SREBP-1 S1P and S2P mutations (U124AFG200-5)

    Plasmid
    #210767
    Purpose
    Mammalian expression of SREBP-1 (isoform 1) with mutations in residues reported to be critical for S1P and S2P cleavage.
    Depositor
    Allan Weissman
    Article
    Yang et al J Biol Chem. 2023 Oct 12;299(12):105346.
    Insert
    SREBP-1 (SREBF1 Human)
    Use
    Tags
    E-tag and T7x2
    Expression
    Mammalian
    Mutation
    DRSR 514-517 to AS; 557 R to A; multiple silent m…
    Promoter
    Available since
    April 8, 2024
    Availability
    Academic Institutions and Nonprofits only
    210767-plasmid-map-sequence-id-421642

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  7. pLKO.1 LARP7-FLAG m6A mut 7SK

    Plasmid
    #200734
    Purpose
    Lentiviral expression of Flag-Tagged human LARP7 and the snRNA 7SK
    Depositor
    Claudio Alarcón
    Insert
    LARP7 and 7SK (LARP7 Human)
    Use
    Lentiviral
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    7SK m6A mutations: A172G, A220T, A228G, A281G, an…
    Promoter
    U6
    Available since
    July 26, 2023
    Availability
    Academic Institutions and Nonprofits only
    200734-plasmid-map-sequence-id-401805

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  8. Human DsRed-Monomer CASP9 C287A Mutant

    Plasmid
    #198469
    Purpose
    Expresses Human DsRed-Monomer CASP9 C287A Mutant Fusion Protein in Mammalian Cells
    Depositor
    Hannah Rabinowich
    Insert
    Human Caspase-9 C287A Mutant (CASP9 Human)
    Use
    Tags
    DsRed-monomer
    Expression
    Mammalian
    Mutation
    Changed Cysteine 287 to Alanine to eliminate prot…
    Promoter
    CMV
    Available since
    April 14, 2023
    Availability
    Academic Institutions and Nonprofits only
    198469-plasmid-map-sequence-id-390261

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  9. N-terminal 3X Flag Human CASP9 C287A Mutant

    Plasmid
    #198380
    Purpose
    Expresses Human N-Terminal 3X Flag CASP9 Catalytic Mutant in Mammalian Cells
    Depositor
    Hannah Rabinowich
    Article
    Han et al Autophagy. 2021 Jun;17(6):1393-1409. doi
    Insert
    N-Terminal 3X Flag Human Caspase-9 C287A Mutant (CASP9 Human)
    Use
    Tags
    3X Flag
    Expression
    Mammalian
    Mutation
    Changed Cysteine 287 to Alanine to eliminate prot…
    Promoter
    CMV
    Available since
    April 14, 2023
    Availability
    Academic Institutions and Nonprofits only
    198380-plasmid-map-sequence-id-390258

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  21. LV TRE-GFP-TMD-DLL1-Nbox-mutant

    Plasmid
    #194937
    Purpose
    LV construct encoding a mutated GFP-TMD-DLL1 ligand containing an ablated Nbox site under dox-inducible TRE promoter and containing a PGK-rtTA-M2-IRES-Puro regulator/resistance marker cassette.
    Depositor
    John Ngo
    Article
    Sloas et al Nat Biotechnol. 2023 Jan 16. doi: 10.103
    Insert
    eGFP-(PDGFR)TMD-rDLL1 ICD-Nbox-AlaX3-mutant
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    194937-plasmid-map-sequence-id-386567

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  24. pAc5.1B-EGFP-DmNot1-3xL-Amut-dsRNAres_P

    Plasmid
    #147160
    Purpose
    Insect Expression of DmNot1-3xL-Amut-dsRNAres
    Depositor
    Elisa Izaurralde
    Insert
    DmNot1-3xL-Amut-dsRNAres (Not1 Fly)
    Use
    Tags
    Expression
    Insect
    Mutation
    10 silent mutations and two non silent N605S, K75…
    Promoter
    Available since
    Jan. 23, 2023
    Availability
    Academic Institutions and Nonprofits only
    147160-plasmid-map-sequence-id-289607

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  25. pAc5.1B-EGFP-DmNot1_1147-1717-3xL-Amut_P

    Plasmid
    #147161
    Purpose
    Insect Expression of DmNot1_1147-1717-3xL-Amut
    Depositor
    Elisa Izaurralde
    Insert
    DmNot1_1147-1717-3xL-Amut (Not1 Fly)
    Use
    Tags
    Expression
    Insect
    Mutation
    2 silent mutations compared to the sequence given…
    Promoter
    Available since
    Jan. 23, 2023
    Availability
    Academic Institutions and Nonprofits only
    147161-plasmid-map-sequence-id-289608

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  26. pAc5.1B-lambdaN-HA-DmNot1-3xL-Amut-dsRNAres_P

    Plasmid
    #147165
    Purpose
    Insect Expression of DmNot1-3xL-Amut-dsRNAres
    Depositor
    Elisa Izaurralde
    Insert
    DmNot1-3xL-Amut-dsRNAres (Not1 Fly)
    Use
    Tags
    Expression
    Insect
    Mutation
    10 silent mutations and two non silent N605S, K75…
    Promoter
    Available since
    Jan. 23, 2023
    Availability
    Academic Institutions and Nonprofits only
    147165-plasmid-map-sequence-id-289612

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  27. pAc5.1B-lambdaN-HA-DmNot1_1147-1386-3xL-Amut_P

    Plasmid
    #147167
    Purpose
    Insect Expression of DmNot1_1147-1386-3xL-Amut
    Depositor
    Elisa Izaurralde
    Insert
    DmNot1_1147-1386-3xL-Amut (Not1 Fly)
    Use
    Tags
    Expression
    Insect
    Mutation
    1 silent mutation compared to the sequence given …
    Promoter
    Available since
    Jan. 23, 2023
    Availability
    Academic Institutions and Nonprofits only
    147167-plasmid-map-sequence-id-289614

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  28. pAc5.1B-lambdaN-HA-DmNot1_1147-1717-3xL-Amut_P

    Plasmid
    #147171
    Purpose
    Insect Expression of DmNot1_1147-1717-3xL-Amut
    Depositor
    Elisa Izaurralde
    Insert
    DmNot1_1147-1717-3xL-Amut (Not1 Fly)
    Use
    Tags
    Expression
    Insect
    Mutation
    2 silent mutations compared to the sequence given…
    Promoter
    Available since
    Jan. 23, 2023
    Availability
    Academic Institutions and Nonprofits only
    147171-plasmid-map-sequence-id-289618

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  30. pTRIP TRE BI_SEPT9_i1-14-GFP10_GFP11-14-SEPT7 Gmut2

    Plasmid
    #180359
    Purpose
    mammalian co-expression of human SEPT9_i1 fused to GFP10 and of human SEPT7 Gmut2 fused to GFP11
    Depositor
    Manos Mavrakis
    Article
    Martins et al J Cell Biol. 2023 Mar 6;222(3):e20220301
    Inserts
    SEPTIN7 (SEPTIN7 Human)
    SEPTIN 9 (SEPTIN9 Human)
    IRES-TagBFP
    Use
    Lentiviral
    Tags
    GFP10 and GFP11
    Expression
    Mammalian
    Mutation
    SEPT7 H279D
    Promoter
    bidirectional TRE
    Available since
    Dec. 1, 2022
    Availability
    Academic Institutions and Nonprofits only
    180359-plasmid-map-sequence-id-358019

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Showing: 1141 - 1170 of 17336 results