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We narrowed to 6,985 results for: ef1a

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pAAV-Ef1a DIO-rsChRmine-oScarlet-WPRE

Plasmid

#183528

Purpose

Optogenetics

Depositor

Karl Deisseroth

Article

Kishi et al Cell. 2022 Feb 17;185(4):672-689.e23. do

Insert

rsChRmine-oScarlet

Use

AAV

Tags

Expression

Mutation

I146M/G174S

Promoter

Ef1a

Available since

May 18, 2022

Availability

Academic Institutions and Nonprofits only

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U6-sgRNA(MS2)_EF1a-MS2-P65-HSF1

Plasmid

#92120

Purpose

Expression plasmid for both MS2-P65-HSF1 activator helper complex and sgRNA with two MS2 loops at tetraloop and stemloop 2 contains BsaI sites for insertion of spacer sequences.

Depositor

Ervin Welker

Article

Kulcsar et al Genome Biol. 2017 Oct 6;18(1):190. doi:

Insert

MS2-P65-HSF1 (HSF1 Synthetic, Human)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Oct. 31, 2017

Availability

Academic Institutions and Nonprofits only

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pHR-UCOE-EF1a-Zim3-dCas9-BFP

Plasmid

#188775

Purpose

dCas9 with an N-term Zim3 KRAB-NLS fusion, a C-term HA-2xNLS

Depositor

Jonathan Weissman, Marco Jost

Article

Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75

Insert

Zim3-dCas9-mTagBFP2

Use

Lentiviral

Tags

HA-2xNLS-mTagBFP2 and Zim3 KRAB-NLS fusion

Expression

Mutation

Promoter

EF1a

Available since

Oct. 18, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1a-mCherry-P2A-NLSFlpN-nMagHigh

Plasmid

#180585

Purpose

PA-Flp

Depositor

Won Do Heo

Article

Jung et al Nat Commun. 2019 Jan 18;10(1):314. doi:

Insert

Split FlpO

Use

AAV

Tags

Expression

Mutation

Promoter

Available since

Feb. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pCF525-EF1a-Hygro-P2A-AcrIIA4-lenti

Plasmid

#115795

Purpose

Expresses AcrIIA4 in human cells, contained in a lentiviral vector.

Depositor

Jennifer Doudna

Article

Watters et al Science. 2018 Oct 12;362(6411):236-239.

Insert

AcrIIA4

Use

Lentiviral

Tags

Expression

Bacterial and Mammalian

Mutation

Promoter

EF-1a promoter

Available since

Oct. 23, 2018

Availability

Academic Institutions and Nonprofits only

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pU6-sgRNA EF1Alpha-puro-T2A-BFP.CYRANO

Plasmid

#128755

Purpose

Expresses CYRANO gRNA for CRISPRi

Depositor

David Bartel

Article

Kleaveland et al Cell. 2018 Jul 12;174(2):350-362.e17. do

Insert

CYRANO sgRNA (OIP5-AS1 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

mU6

Available since

Aug. 14, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-Ef1a-DIO-ChETA-TdTomato-WPRE-pA

Plasmid

#37755

Purpose

Cre-dependent expression of ChETA-tdTomato, for optogenetic excitation

Depositor

Bernardo Sabatini

Article

Saunders et al Front Neural Circuits. 2012 Jul 27;6:47.

Insert

ChETA-TdTomato

Use

AAV and Cre/Lox; Cre-on

Tags

TdTomato

Expression

Mutation

Promoter

EF1a

Available since

July 27, 2012

Availability

Academic Institutions and Nonprofits only

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CAPTURE2_pLVX-EF1a-dCas9-CBio-IRES-zsGreen1

Plasmid

#138418

Purpose

CAPTURE2.0 vector containing dCas9-CBio, IRES and zsGreen1

Depositor

Jian Xu

Article

Liu et al Genome Biol. 2020 Mar 5;21(1):59. doi: 1

Insert

dCas9

Use

Lentiviral

Tags

BioTAP-tag

Expression

Mutation

Promoter

EF1a

Available since

July 9, 2020

Availability

Academic Institutions and Nonprofits only

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pU6-sgRNA Ef1alpha Puro-T2A-GFP

Plasmid

#111596

Purpose

sgRNA Ef1alpha Puro-T2A-GFP

Depositor

Jonathan Weissman

Article

Horlbeck et al Cell. 2018 Aug 9;174(4):953-967.e22. doi

Insert

mU6-sgRNA

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-Ef1a-DIO eNpHR 3.0-EYFP

Plasmid

#26966

Purpose

AAV expression of eNpHR 3.0 fused to EYFP driven by EF1a promoter for optogenetic inhibition

Depositor

Karl Deisseroth

Article

Gradinaru et al Cell. 2010 Apr 2. 141(1):154-65.

Has Service

AAV Retrograde, AAV1, AAV5, and AAV9

Insert

eNpHR 3.0

Use

AAV

Tags

EYFP

Expression

Mammalian

Mutation

ER export at 3' end of gene. Trafficking sig…

Promoter

EF1alpha

Available since

Jan. 27, 2011

Availability

Academic Institutions and Nonprofits only

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pHR hEF1A-BFP-P2A-rtTA-WPRE

Plasmid

#223288

Purpose

A lentiviral vector expressing BFP and the TetON transcription factor rtTA.

Depositor

Jared Toettcher

Article

Brumbaugh-Reed et al bioRxiv 2024.01.16.575860

Insert

BFP-P2A-rtTA

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 14, 2024

Availability

Academic Institutions and Nonprofits only

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pLVX-EF1alpha-eGFP-2xStrep-IRES-Puro

Plasmid

#141395

Purpose

Lentiviral expression of SARS-CoV-2 protein; transient expression and generate lentivirus

Depositor

Nevan Krogan

Article

Gordon et al Nature. 2020 Jul;583(7816):459-468. doi:

Insert

eGFP

Use

Lentiviral

Tags

2xStrep

Expression

Mammalian

Mutation

human codon optimized

Promoter

EF1alpha

Available since

April 13, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-Ef1a-fDIO-eGFP-2A-Cre-WPRE

Plasmid

#203842

Purpose

Flp-dependent Cre and EGFP expression

Depositor

Ivo Spiegel

Article

Roethler et al Neuron. 2023 Jun 20:S0896-6273(23)00399-

Insert

Cre

Use

AAV

Tags

EGFP-2A

Expression

Mutation

Promoter

Available since

Aug. 9, 2023

Availability

Academic Institutions and Nonprofits only

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pLV-EF1a-mTagBFP2-2A-Puro_hU6-DJ-DR36-BsmBI

Plasmid

#226009

Purpose

DjCas13d guide RNA cloning backbone (lenti)

Depositor

Silvana Konermann

Article

Wei et al Cell Syst. 2023 Dec 20;14(12):1087-1102.

Type

Empty backbone

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Oct. 17, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-EF1A-mNeonGreen2x-SREBP1-P2A-Puro

Plasmid

#228401

Purpose

Encodes a N-terminal truncated version of SREBP1 lacking part of the transcriptional activation domain and fused to a tandem of mNeongreen to visualize the import of SREBP1

Depositor

Dirk Daelemans

Article

Vercruysse et al Commun Biol. 2024 Nov 23;7(1):1560. doi:

Insert

SREBP1 (SREBF1 Human)

Use

Tags

mNeonGreen2x

Expression

Mammalian

Mutation

deleted N terminal activation domain (amino acids…

Promoter

EF1a

Available since

Jan. 8, 2025

Availability

Academic Institutions and Nonprofits only

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p2706-pHAGE-EF1aL-YAP5SA-UBC-GFP

Plasmid

#216475

Purpose

lentiviral dual expression of YAP5SA and eGFP

Depositor

Darrell Kotton

Article

Burgess et al Cell Stem Cell. 2024 Apr 15:S1934-5909(2

Inserts

YAP5SA
eGFP

Use

Lentiviral

Tags

Expression

Mutation

S61A, S109A, S127A, S164A, S381A

Promoter

EF1aL and UBC

Available since

April 22, 2024

Availability

Academic Institutions and Nonprofits only

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pLV-Hygro-EF1A-BRCT2-Linker-eGFP

Plasmid

#176085

Purpose

EGFP fused to the C-terminus of a BRCT2 domain & a hygromycin resistance cassette

Depositor

Robert Sobol

Article

Koczor et al Cell Rep. 2021 Nov 2;37(5):109917. doi:

Insert

BRCT2 (XRCC1 Human)

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

EF1A

Available since

Jan. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pLV-Hygro-EF1A-BRCT1-Linker-eGFP

Plasmid

#176074

Purpose

EGFP fused to the C-terminus of a BRCT1 domain & a hygromycin resistance cassette

Depositor

Robert Sobol

Article

Koczor et al Cell Rep. 2021 Nov 2;37(5):109917. doi:

Insert

BRCT1 (XRCC1 Human)

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

EF1A

Available since

Jan. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pLV-Hygro-EF1A-FHA-Linker-eGFP

Plasmid

#176065

Purpose

EGFP fused to the C-terminus of a FHA domain & a hygromycin resistance cassette

Depositor

Robert Sobol

Article

Koczor et al Cell Rep. 2021 Nov 2;37(5):109917. doi:

Insert

FHA

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

EF1A

Available since

Jan. 24, 2022

Availability

Academic Institutions and Nonprofits only

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AAV pEF1a-DIO-FLPo-WPRE-hGHpA

Plasmid

#87306

Purpose

An AAV vector expresses FLPo in a Cre recombinase-dependent manner.

Depositor

Li Zhang

Article

Zingg et al Neuron. 2017 Jan 4;93(1):33-47. doi: 10.

Has Service

AAV Retrograde, AAV1, AAV2, AAV5, AAV8, and AAV9

Insert

Cre-dependent mouse codon-optimized Flp recombinase.

Use

AAV

Tags

N/A

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

March 7, 2017

Availability

Academic Institutions and Nonprofits only

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pLV-EF1a-mCherry-MinD-IRES-Puro

Plasmid

#213112

Purpose

Expression of mCherry-MinD in mammalian cells

Depositor

Scott Coyle

Article

Rajasekaran et al Cell. 2023 Dec 27:S0092-8674(23)01339-9.

Insert

mCherry-MinD (minD Escherichia coli)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Jan. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pCF525-EF1a-Hygro-P2A-mCherry-lenti

Plasmid

#115796

Purpose

Expresses mCherry in human cells, contained in a lentiviral vector.

Depositor

Jennifer Doudna

Article

Watters et al Science. 2018 Oct 12;362(6411):236-239.

Insert

mCherry

Use

Lentiviral

Tags

Expression

Bacterial and Mammalian

Mutation

Promoter

EF-1a promoter

Available since

Oct. 19, 2018

Availability

Academic Institutions and Nonprofits only

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pOTTC469 - pAAV EF1a DIO eNpHR3.0-iRFP

Plasmid

#47631

Purpose

An AAV vector that expresses engineered halorhodopsin 3.0 (fused to iRFP) in a Cre-dependent manner (DIO, double floxed inverted open reading frame).

Depositor

Brandon Harvey

Article

Richie et al J Neurosci Methods. 2017 Jun 1;284:1-14.

Insert

eNpHR3.0

Use

AAV and Cre/Lox

Tags

iRFP

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Sept. 12, 2013

Availability

Academic Institutions and Nonprofits only

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pLVX-EF1a-EGFP-TA Golgin84-IRES-Puromycin

Plasmid

#133028

Purpose

Expresses EGFP-tagged Golgi apparatus marker

Depositor

David Andrews

Article

Schormann et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

Insert

Golgin84 (GOLGA5 Human)

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Mutation

deleted amino acids 1-673

Promoter

Human elongation factor 1 alpha

Available since

Feb. 7, 2020

Availability

Academic Institutions and Nonprofits only

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AAV-EF1A-DIO-Gephyrin.FingR-eGFP-CCR5TC

Plasmid

#126217

Purpose

Labeling of inhibitory synapses (green fluorescence) in cre-expressing neurons

Depositor

Xue Han

Article

Bensussen et al iScience. 2020 Jun 30;23(7):101330. doi:

Insert

Gephyrin.FingR-eGFP-CCR5TC

Use

AAV and Cre/Lox

Tags

Expression

Mammalian

Mutation

Promoter

EF1A

Available since

July 2, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-Ef1a-DIO ChRmine-eYFP-WPRE

Plasmid

#130996

Purpose

Double floxed ChRmine-eYFP under the control of Ef1a promoter

Depositor

Karl Deisseroth

Article

Marshel et al Science. 2019 Aug 9;365(6453). pii: scie

Insert

ChRmine

Use

AAV

Tags

eYFP

Expression

Mutation

Promoter

Ef1a

Available since

Sept. 11, 2019

Availability

Academic Institutions and Nonprofits only

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pLVX-EF1a-EGFP-TA MAO-IRES-Puromycin

Plasmid

#133025

Purpose

Expresses EGFP-tagged tail anchor sequence of monoamine oxidase A

Depositor

David Andrews

Article

Schormann et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

Insert

Monoamine oxidase type A (MAOA Human)

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Mutation

deleted amino acids 1-488

Promoter

Human elongation factor 1 alpha

Available since

Feb. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pHAGE2-EF1a-4xGFP11-SEC61B-IRES-Hygro

Plasmid

#211468

Purpose

Lentivirus backbone expressing SEC61B protein tagged with 4 tandem GFP11 repeats at the N-terminal

Depositor

Agnel Sfeir

Article

Fu et al Mol Cell. 2023 Oct 8:S1097-2765(23)00753

Insert

4xGFP11-SEC61B

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF-1a

Available since

Dec. 21, 2023

Availability

Academic Institutions and Nonprofits only

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AAV-EF1A-PSD95.FingR-eGFP-CCR5TC

Plasmid

#125691

Purpose

Global labeling of excitatory synapses (green fluorescence)

Depositor

Xue Han

Article

Bensussen et al iScience. 2020 Jun 30;23(7):101330. doi:

Insert

PSD95.FingR-eGFP-CCR5TC

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

EF1A

Available since

July 2, 2020

Availability

Academic Institutions and Nonprofits only

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pLVX-EF1a-EGFP-VAMP5-IRES-Puromycin

Plasmid

#134662

Purpose

Expresses EGFP-tagged plasma membrane marker

Depositor

David Andrews

Article

Schormann et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

Insert

vesicle-associated membrane protein 5 (VAMP5 Human)

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

March 2, 2020

Availability

Academic Institutions and Nonprofits only

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