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We narrowed to 171,128 results for: addgene

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Showing: 1521 - 1560 of 171128 results

  1. pLV hUbC- VP64-dCas9-VP64-T2A- GFP(No LoxP sites)

    Plasmid
    #66708
    Purpose
    expresses VP64-dCas9-VP64, same plasmid as Addgene 59791 but with LoxP sites removed
    Depositor
    Charles Gersbach
    Insert
    S.Pyogenes VP64-dCas9-VP64
    Use
    Lentiviral and Synthetic Biology
    Tags
    VP64
    Mutation
    D10A and H840A
    Promoter
    hUbC
    Available Since
    April 12, 2019
    Availability
    Academic Institutions and Nonprofits only
    66708-plasmid-map-sequence-id-449286

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  3. 6CC8 (MBD3)

    Plasmid
    #119959
    Purpose
    Bacterial expression for structure determination
    Depositor
    Cheryl Arrowsmith
    Insert
    MBD3 (MBD3 Human)
    Tags
    GSTtag, 6xHis and Thrombin
    Expression
    Bacterial
    Mutation
    MBD domain (contains residues 1-71)
    Promoter
    T7
    Available Since
    Jan. 8, 2019
    Availability
    Industry, Academic Institutions, and Nonprofits
    119959-plasmid-map-sequence-id-232658

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  4. AMPKA2

    Plasmid
    #36194
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    AMPKA2 (PRKAA2 Human)
    Tags
    His tag with thrombin cleavage
    Expression
    Bacterial
    Mutation
    contains KINASE DOMAIN only
    Available Since
    Aug. 8, 2012
    Availability
    Industry, Academic Institutions, and Nonprofits

  6. 5T5G (SETD8)

    Plasmid
    #101886
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    SETD8 (KMT5A Human)
    Tags
    6xHis, TEV
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    April 30, 2018
    Availability
    Industry, Academic Institutions, and Nonprofits
    101886-plasmid-map-sequence-id-194755

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  14. COVID-SARS2 NSP16/10

    Plasmid
    #159615
    Purpose
    Bacterial co-expression vector fo Covid-SARS2 NSP16 and NSP10
    Depositor
    Opher Gileadi
    Inserts
    Covid-SARS2 Nsp16
    Covid-SARS2 Nsp10
    Tags
    His6-Zb-TEV and none
    Expression
    Bacterial
    Mutation
    Codon-optimized for E. coli expression
    Promoter
    (Bicistronic) and T7 - LacO
    Available Since
    Sept. 15, 2020
    Availability
    Academic Institutions and Nonprofits only
    159615-plasmid-map-sequence-id-311066

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  16. JAK3 (JAK3A-c023)

    Plasmid
    #98229
    Purpose
    Baculovirus expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    JAK3A (JAK3 Human)
    Use
    Baculovirus
    Tags
    His-6-TEV
    Mutation
    pfam00069:Pkinase 822-1091
    Promoter
    polyhedrin
    Available Since
    Sept. 14, 2017
    Availability
    Academic Institutions and Nonprofits only
    98229-plasmid-map-sequence-id-193400

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  18. DCAF1:PBC020-D05:C246337

    Plasmid
    #183974
    Purpose
    Baculovirus expression for structure determination. May not contain entire coding region of gene.
    Depositor
    Cheryl Arrowsmith
    Insert
    DCAF1:PBC020-D05:C246337 (DCAF1 Human)
    Use
    Baculovirus expresssion
    Tags
    6xHis-TEV and MHHHHHHSSGRENLYFQG
    Promoter
    Polyhedrin
    Available Since
    May 10, 2022
    Availability
    Industry, Academic Institutions, and Nonprofits
    183974-plasmid-map-sequence-id-361427

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  19. DOT1L

    Plasmid
    #36196
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    DOT1L (GeneScript Synthesized) (DOT1L Human)
    Tags
    His tag with TEV cleavage
    Expression
    Bacterial
    Mutation
    contains amino acid residues 1-420
    Available Since
    Aug. 8, 2012
    Availability
    Industry, Academic Institutions, and Nonprofits

  20. 6CCR (GID4)

    Plasmid
    #119960
    Purpose
    Bacterial expression for structure determination
    Depositor
    Cheryl Arrowsmith
    Insert
    GID4 (C17orf39 Human)
    Tags
    6xHIS, thrombin,SUMO and TEV cleavage
    Expression
    Bacterial
    Mutation
    contains amino acids 116-300
    Promoter
    T7
    Available Since
    Jan. 8, 2019
    Availability
    Industry, Academic Institutions, and Nonprofits
    119960-plasmid-map-sequence-id-232678

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  25. ACE2 g4 + Cas9

    Plasmid
    #153014
    Purpose
    A guide RNA targeting ACE2 in a lentiviral plasmid co-expressing Cas9 and TagBFP2
    Depositor
    Jason Sheltzer
    Insert
    Cas9 + ACE2 gRNA (ACE2 Human)
    Use
    Lentiviral
    Tags
    TagBFP2
    Available Since
    June 1, 2020
    Availability
    Academic Institutions and Nonprofits only
    153014-plasmid-map-sequence-id-298677

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  29. C1QBP

    Plasmid
    #32863
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    C1QBP (C1QBP Human)
    Tags
    His
    Expression
    Bacterial
    Mutation
    amino acids E89-Q282 only
    Available Since
    Jan. 6, 2012
    Availability
    Industry, Academic Institutions, and Nonprofits

  31. pCMV-T7-SpCas9-BPNLS(SV40)-3xFLAG-P2A-mScarlet (MNW001)

    Plasmid
    #174091
    Purpose
    CMV and T7 promoter expression plasmid for human codon optimized SpCas9 with a C-terminal bi-partite NLS, 3x flag tag, and P2A-mScarlet
    Depositor
    Benjamin Kleinstiver
    Insert
    human codon optimized SpCas9 with BPNLS-3xFLAG-P2A-mScarlet
    Use
    In vitro transcription; t7 promoter
    Tags
    BPNLS-3xFLAG-P2A-mScarlet
    Expression
    Mammalian
    Promoter
    pCMV and T7
    Available Since
    Sept. 8, 2021
    Availability
    Academic Institutions and Nonprofits only
    174091-plasmid-map-sequence-id-341917

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  32. 6CDG (GID4)

    Plasmid
    #119961
    Purpose
    Bacterial expression for structure determination
    Depositor
    Cheryl Arrowsmith
    Insert
    GID4 (C17orf39 Human)
    Tags
    6xHis-TEV
    Expression
    Bacterial
    Mutation
    contains amino acids 124-289
    Promoter
    T7
    Available Since
    Jan. 8, 2019
    Availability
    Industry, Academic Institutions, and Nonprofits
    119961-plasmid-map-sequence-id-232684

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  33. XLone-Puro SARS-CoV2 N P2A eGFP-NLS

    Plasmid
    #154399
    Purpose
    PiggybacTransposon-based tunable and temporal expression control of SARS-CoV2 N and nuclear eGFP
    Depositor
    Xiaoping Bao
    Insert
    SARS-CoV2 N Protein (N SARS-CoV2)
    Use
    Piggybac
    Tags
    mCherry
    Expression
    Mammalian
    Promoter
    TRE3G
    Available Since
    June 30, 2020
    Availability
    Academic Institutions and Nonprofits only
    154399-plasmid-map-sequence-id-304590

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  40. AKAP13

    Plasmid
    #73232
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    AKAP13 (AKAP13 Human)
    Tags
    His-6-TEV
    Expression
    Bacterial
    Mutation
    contains amino acids 1976-2211
    Promoter
    T7
    Available Since
    April 21, 2016
    Availability
    Academic Institutions and Nonprofits only
Showing: 1521 - 1560 of 171128 results