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pMal mPtch-Cterm
Plasmid#58705Purposeused for anti-mouse Patched antibody production (for affinity purification)DepositorInsertPtch (Ptch1 Mouse)
UseTagsMBPExpressionBacterialMutationcontains only Cterminus (aa ~1205-1434)PromoterAvailable sinceJuly 22, 2014AvailabilityAcademic Institutions and Nonprofits only -
pGGB005
Plasmid#48822PurposeProvides the SV40 nuclear localization signal as GreenGate N-terminal tag module.DepositorInsertSV40 nuclear localization signal
UseGolden gate compatible cloning vectorTagsExpressionMutationPromoterAvailable sinceJan. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
zw4 (egghead) cDNA#7
Plasmid#37398DepositorInsertegghead (egh Fly)
UseTagsExpressionMutationPromoterAvailable sinceJuly 31, 2012AvailabilityAcademic Institutions and Nonprofits only -
p6671 MSCV-IP N-HAonly 38E6
Plasmid#37056DepositorInsertHPV38 E6
UseRetroviralTagsHAExpressionMammalianMutationPromoterMSCV LTRAvailable sinceJuly 16, 2012AvailabilityAcademic Institutions and Nonprofits only -
zw4 (egghead) cDNA#4e
Plasmid#37399DepositorInsertegghead (egh Fly)
UseTagsExpressionMutationPromoterAvailable sinceJuly 16, 2012AvailabilityAcademic Institutions and Nonprofits only -
XE74 TM Beta-catenin 1-9 in CS2+
Plasmid#16832DepositorInsertTM beta-catenin 1-9 (ctnnb1.L Frog)
UseXenopus expressionTagsExpressionMutationPromoterAvailable sinceMarch 13, 2008AvailabilityAcademic Institutions and Nonprofits only -
ZP59 23f17
Plasmid#16883DepositorInsert23f17
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 1, 2008AvailabilityAcademic Institutions and Nonprofits only -
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Axin AID/pCS2HA
Plasmid#16308DepositorInsertAxin Axin Interaction Domain (axin1.L Frog)
UseVertebrate expressionTagsHAExpressionMutationAID (Axin interaction domain): aa 510-777PromoterAvailable sinceDec. 21, 2007AvailabilityAcademic Institutions and Nonprofits only -
pBS mouse IRS3 promoter
Plasmid#11444DepositorInsertIRS-3 promoter (Irs3 Mouse)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 3, 2006AvailabilityAcademic Institutions and Nonprofits only -
pMT FRB-Draper INT-mCherry
Plasmid#126666PurposeInducible expression of a synthetic FRB-Draper receptor in S2 cellsDepositorInsertFRB-Draper INT domain mCherry
UseTagsExpressionInsectMutationPromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pLJM1-EGFP
Plasmid#19319Purpose3rd gen lentiviral vector for EGFP fusion; PGK driven puromycinDepositorHas ServiceCloning Grade DNATypeEmpty backboneUseLentiviralTagsEGFPExpressionMammalianMutationPromoterAvailable sinceSept. 15, 2008AvailabilityAcademic Institutions and Nonprofits only -
TATA-GFP
Plasmid#110833Purposenegative controlDepositorInsertGFP
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceMay 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHR-scFv-GCN4-sfGFP-GB1-dWPRE
Plasmid#60907PurposeThe plasmid encodes an antibody that binds to the GCN4 peptide from the SunTag system, and is fused to sfGFP for imagingDepositorInsertscFv-GCN4
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJan. 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
HyPer7.2DAAO-NES
Plasmid#168301PurposeChemogenetic fluorescent H2O2 reporter targeted to the cytoplasmDepositorInsertHyPer7.2DAAO-NES
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMay 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
EGFP-PIP5K1A
Plasmid#202720Purposeexpresses fluorescent protein-tagged enzymeDepositorInsertEGFP:PIP5K1A (PIP5K1A Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
TagBFP2-PIP4K2A
Plasmid#202726Purposeexpresses fluorescent protein-tagged enzymeDepositorInsertmTagBFP2:PIP4K2A (PIP4K2A Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
TagBFP2-PIP4K2B
Plasmid#202728Purposeexpresses fluorescent protein-tagged enzymeDepositorInsertmTagBFP2:PIP4K2B (PIP4K2B Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
Lyn-tailed mCherry-SEpHluorin
Plasmid#32002DepositorInsertMembrane-targeting sequence of Lyn kinase
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 9, 2012AvailabilityAcademic Institutions and Nonprofits only -
TagBFP2- PIP4K2BD278K
Plasmid#202729Purposeexpresses fluorescent protein-tagged enzymeDepositorInsertmTagBFP2:PIP4K2B (D278K) (PIP4K2B Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
EGFP-supervillin
Plasmid#13040DepositorInsertsupervillin (SVIL Bovine)
UseTagsEGFPExpressionMammalianMutationPromoterAvailable sinceSept. 26, 2006AvailabilityAcademic Institutions and Nonprofits only -
pET29-bdSUMO-Anti-ALFA nanobody
Plasmid#212311PurposeExpression of anti-ALFA nanobodyDepositorInsertanti-ALFA nanobody
UseTagsbdSUMOExpressionBacterialMutationPromoterT7Available sinceApril 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTP1122
Plasmid#104159PurposeBacterial expression plasmid of anti-mouse IgG1 Fc nanobody TP1107 (3x Cysteines)DepositorInsertAnti-mouse IgG1 Fc specific nanobody TP1107 (3xCysteines)
UseTags14xHistidine tag and NEDD8 from Brachypodium dist…ExpressionBacterialMutationPromoterAvailable sinceDec. 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTP955
Plasmid#104164PurposeBacterial expression plasmid of anti-rabbit IgG Fc nanobody TP897 (3x Cysteines)DepositorInsertAnti-rabbit IgG Fc specific nanobody TP897 (3xCysteines)
UseTags14xHistidine tag and NEDD8 from Brachypodium dist…ExpressionBacterialMutationPromoterAvailable sinceDec. 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
human syndecan 2 wt
Plasmid#64970PurposeConstitutive expression in mammalian cellsDepositorInsertsyndecan 2 (SDC2 Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJune 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pelBHisTEV
Plasmid#157741Purposeempty vector; TEV-protease-removable N-term PelB signal sequence +His10; potential C-term His6; T7lac promoter; Kan-R; avoids added residues in final protein since cloning uses BseRI restriction sitesDepositorTypeEmpty backboneUseTagsPelB signal sequence + His10-tag + TEV protease c…ExpressionBacterialMutationPromoterT7lacAvailable sinceJune 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
JGE302_pAAV-hSyn-DIO-Myc-PYLcs-bArrestin2-YFP (p2)
Plasmid#107247Purposebeta arrestin 2 fused to PYLcs for abscisic acid mediated recruitment of ABIcs labelled proteins.DepositorArticleInsertARRB2
UseAAVTagsMYC, PYLcs, and YFPExpressionMutationPromoterhSYNAvailable sinceApril 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pPBpuro-4493NES(Booster-PKA)
Plasmid#184709PurposePKA biosensorDepositorInsertBooster-PKA
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pC1-CMV-DAAO-NES
Plasmid#141107PurposeExpresses DAAO with nuclear export signal in mammalian cellsDepositorInsertDAAO-NES
UseTagsNuclear export signalExpressionMammalianMutationdeleted DAAO amino acids 367-368PromoterCMVAvailable sinceFeb. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pVITRO1-TagRFP-NLS
Plasmid#184446PurposeNucleus-targeted TagRFP expression in mammalian cellsDepositorInsertTagRFP-3xNLS
UseTagsExpressionMammalianMutationWTPromoterRat EF1αAvailable sinceJuly 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pVITRO1-mCardinal-NES
Plasmid#184453PurposeCytosolic mCardinal expression in mammalian cellsDepositorInsertmCardinal-NES
UseTagsExpressionMammalianMutationWTPromoterRat EF1αAvailable sinceMay 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHR-scFv-GCN4-sfGFP-GB1-NLS-dWPRE
Plasmid#60906PurposeThe plasmid encodes an antibody that binds to the GCN4 peptide from the SunTag system, and is fused to sfGFP and a NLS for imagingDepositorInsertscFv-GCN4
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceNov. 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
NbAlfa-GFP2
Plasmid#201485PurposeBRET2 detection of ALFA-tagged proteinDepositorInsertNbAlfa-GFP2
UseTagsGFP2ExpressionMammalianMutationPromoterCMVAvailable sinceAug. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
HyPer7.2DAAO-mito
Plasmid#168304PurposeChemogenetic fluorescent H2O2 reporter targeted to the mitochondriaDepositorInsertHyPer7.2DAAO-mito
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMay 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-Peredox
Plasmid#73807PurposeExpresses the Peredox fluorescent sensor protein (for NADH/NAD ratio) in mammalian cells (and can be packaged as AAV)DepositorInsertPeredox
UseAAVTagsExpressionMammalianMutationPromoterAvailable sinceMarch 30, 2016AvailabilityAcademic Institutions and Nonprofits only -
V245 pCEP-4HA B56alpha
Plasmid#14532DepositorInsertPPP2R5A B56 alpha regulatory subunit of PP2A (PPP2R5A Human)
UseTags4 HAExpressionMammalianMutationPromoterAvailable sinceMarch 23, 2007AvailabilityAcademic Institutions and Nonprofits only -
pcDNA4TO-K560-E236A-24xGCN4_v1-IRES-Puro
Plasmid#60909PurposeA minimal K560 kinesin rigor motor tagged with the SunTag for single molecule imagingDepositorInsertK560-E236A
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
pBL4
Plasmid#50548PurposeExpresses CcaS and CcaR constitutively, and sfGFP/TetR under the PcpcG2 promoterDepositorInsertsCcaS
CcaR
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceJune 24, 2014AvailabilityAcademic Institutions and Nonprofits only -
MPZ(P0)promoter pGL3-TATA
Plasmid#21259DepositorInsertmyelin protein zero promoter (Mpz Mouse)
UseLuciferaseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 13, 2009AvailabilityAcademic Institutions and Nonprofits only -
CMVTO-GFP-ECL-3TM-tevs-KKMP V3
Plasmid#179640PurposeTEVP-inducible membrane-bound GFP RELEASE constructDepositorInsertMembrane-bound GFP RELEASE
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-PKA-SPARK
Plasmid#106920PurposeExpress PKA-SPARK, an activity reporter of protein kinase A in mammalian cells.DepositorInsertPKA-SPARK
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-EFS-MCP-HALOnls
Plasmid#121937PurposeCRISPR-Sirius plasmidDepositorInsertMCP-HALOnls
UseLentiviralTagsHaloTagExpressionMammalianMutationPromoterEFSAvailable sinceFeb. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-ERK-SPARK
Plasmid#106921PurposeExpress ERK-SPARK, an activity reporter of ERK in mammalian cells.DepositorInsertERK-SPARK
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-EFS-PCP-GFPnls
Plasmid#121938PurposeCRISPR-Sirius plasmidDepositorInsertPCP-GFPnls
UseLentiviralTagssfGFPExpressionMammalianMutationPromoterEFSAvailable sinceFeb. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCambia-PUP-IT
Plasmid#186478PurposeExpresses POI-PUP-IT fusion in PlantsDepositorInsertFLAG-Pup(E)
UseTagsTriple Flag TagExpressionPlantMutationPromoter35SAvailable sinceSept. 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
GFP2-NbALFA
Plasmid#201486PurposeBRET2 detection of ALFA-tagged proteinDepositorInsertGFP2-NbALFA
UseTagsGFP2ExpressionMammalianMutationPromoterCMVAvailable sinceAug. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
NbV5-eGFP
Plasmid#201473PurposeIndirect microscopy/imaging of V5-tagged proteinDepositorInsertNbV5-eGFP
UseTagseGFPExpressionMammalianMutationPromoterCMVAvailable sinceAug. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRK7-FLAG-TSC1
Plasmid#8995DepositorInsertTSC1 (TSC1 Human)
UseTagsFLAGExpressionMammalianMutationPromoterAvailable sinceNov. 11, 2005AvailabilityAcademic Institutions and Nonprofits only -
pEF.myc.ER-E2-Crimson
Plasmid#38770PurposeIn vivo visualization of the endoplasmic reticulum (can be used for colocalization studies)DepositorInsertE2-Crimson
UseTagsER retention signal (KDEL), ER signal sequence (M…ExpressionMammalianMutationPromoterEF-1aAvailable sinceAug. 15, 2012AvailabilityAcademic Institutions and Nonprofits only
