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pSpCas9(BB)-2A-puro-hANKRD11 (exon 5-1)

Plasmid

#236612

Purpose

Knockouts ANKRD11 in human cells

Depositor

Tadashi Nakagawa

Article

Sashiyama et al iScience. 2025 May 19;28(6):112699. doi:

Insert

ANKRD11 (ANKRD11 Human)

Use

CRISPR

Expression

Mammalian

Available Since

June 6, 2025

Availability

Academic Institutions and Nonprofits only

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pSpCas9(BB)-2A-puro-mAnkrd11 (intron 3)

Plasmid

#236608

Purpose

Knockouts Ankrd11 in mouse cells

Depositor

Tadashi Nakagawa

Article

Sashiyama et al iScience. 2025 May 19;28(6):112699. doi:

Insert

Ankrd11 (Ankrd11 Mouse)

Use

CRISPR

Expression

Mammalian

Available Since

June 2, 2025

Availability

Academic Institutions and Nonprofits only

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CIRC#del

Plasmid

#186344

Purpose

Contains VEGFA exon 7 and short intronic flanking regions not sufficient for expression of hsa_circ_0076611 (circVEGF)

Depositor

Giovanni Blandino

Article

Turco et al Commun Biol. 2022 Jun 16;5(1):598. doi:

Insert

VEGFA (VEGFA Human)

Expression

Mammalian

Available Since

Oct. 17, 2024

Availability

Academic Institutions and Nonprofits only

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CIRC

Plasmid

#186343

Purpose

Contains VEGFA exon 7 and part of intronic flanking regions enabling expression of hsa_circ_0076611 (circVEGF)

Depositor

Giovanni Blandino

Article

Turco et al Commun Biol. 2022 Jun 16;5(1):598. doi:

Insert

VEGFA (VEGFA Human)

Expression

Mammalian

Available Since

July 13, 2022

Availability

Academic Institutions and Nonprofits only

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pMIR-Report-SPRED1-3'UTR (mut - miR-21 site1)

Plasmid

#62580

Purpose

Translational Luciferase Reporter encoding a mutated fragment of the SPRED1 3'UTR. The upstream miR-21 (site 1) binding site was mutated.

Depositor

Michael Ruppert

Article

Sharma et al Mol Cell Biol. 2014 Nov 15;34(22):4143-6

Insert

SPRED1 (SPRED1 Human)

Use

Luciferase

Mutation

miR-21 binding site 1 mutant (TAAGCTA --> TAGA…

Available Since

June 4, 2015

Availability

Academic Institutions and Nonprofits only
pMIR-Report-SPRED1-3'UTR (mut - miR-21 site 2)

Plasmid

#62581

Purpose

Translational Luciferase Reporter encoding a mutated fragment of the SPRED1 3'UTR. The downstream miR-21 binding site (site 2) was mutated.

Depositor

Michael Ruppert

Article

Sharma et al Mol Cell Biol. 2014 Nov 15;34(22):4143-6

Insert

SPRED1 (SPRED1 Human)

Use

Luciferase

Mutation

miR-21 binding site 2 mutant (TAAGCTA --> TAGA…

Available Since

June 4, 2015

Availability

Academic Institutions and Nonprofits only
MSCV-human Erbb2-IRES-GFP

Plasmid

#91888

Purpose

Retroviral expression of human Erbb2

Depositor

Martine Roussel

Article

Vo et al Cell Rep. 2017 Mar 21;18(12):2907-2917.

Insert

Erbb2 (ERBB2 Human)

Use

Retroviral

Tags

IRES-EGFP

Expression

Mammalian

Promoter

MMLV gag

Available Since

July 6, 2017

Availability

Academic Institutions and Nonprofits only

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MSCV-mouse Ezh2 Y637S-IRES-GFP

Plasmid

#91887

Purpose

Retroviral expression of mouse Ezh2 with Y637S

Depositor

Martine Roussel

Article

Vo et al Cell Rep. 2017 Mar 21;18(12):2907-2917.

Insert

Ezh2 (Ezh2 Mouse)

Use

Retroviral

Tags

IRES-EGFP

Expression

Mammalian

Mutation

Y637S

Promoter

MMLV gag

Available Since

July 6, 2017

Availability

Academic Institutions and Nonprofits only

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MSCV-mouse Ezh2 delta SETdomain-IRES-GFP

Plasmid

#91885

Purpose

Retroviral expression of mouse Ezh2 with a SET domain deletion

Depositor

Martine Roussel

Article

Vo et al Cell Rep. 2017 Mar 21;18(12):2907-2917.

Insert

Ezh2 (Ezh2 Mouse)

Use

Retroviral

Tags

IRES-EGFP

Expression

Mammalian

Mutation

SET domain deleted

Promoter

MMLV gag

Available Since

June 26, 2017

Availability

Academic Institutions and Nonprofits only

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AOI-WT-Cas9-sg-mouse Ezh2-E10-GFP

Plasmid

#91879

Purpose

Expresses 3xFLAG-Cas9 and a gRNA targeting mouse Ezh2

Depositor

Martine Roussel

Article

Vo et al Cell Rep. 2017 Mar 21;18(12):2907-2917.

Insert

gRNA targeting Ezh2 (Ezh2 Mouse)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

June 26, 2017

Availability

Academic Institutions and Nonprofits only
AOI-WT-Cas9-sg-mouse Ezh2-E18-GFP

Plasmid

#91880

Purpose

Expresses 3xFLAG-Cas9 and a gRNA targeting mouse Ezh2

Depositor

Martine Roussel

Article

Vo et al Cell Rep. 2017 Mar 21;18(12):2907-2917.

Insert

gRNA targeting Ezh2 (Ezh2 Mouse)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

June 26, 2017

Availability

Academic Institutions and Nonprofits only
GP1BB

Plasmid

#51908

Purpose

Expresses full-length Platelet glycoprotein Ib beta chain precursor ectodomain in mammalian cells. Stop codon before C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

Depositor

Gavin Wright

Article

Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

Insert

GP1BB (GP1BB Human)

Tags

His tag, enzymatic biotinylation sequence, and ra…

Expression

Mammalian

Mutation

Stop Codon at amino acid 149, before CD4, bio and…

Promoter

CMV

Available Since

Feb. 27, 2015

Availability

Academic Institutions and Nonprofits only

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pCMV6-AN-DDK_ NLS~K682A_K686A_K694_K709A (4KA) POLH

Plasmid

#246406

Purpose

Expresses K682A_K686A_K694_K709A POLH with an N-terminal NLS~FLAG tag in mammalian cells

Depositor

Roger Woodgate

Article

Moreno et al Nucleic Acids Res. 2026 Feb 5;54(4):gkag

Insert

POLH (POLH Human)

Tags

FLAG

Expression

Mammalian

Mutation

POLH K682A_K686A_K694_K709A. Coding sequence has …

Available Since

Feb. 23, 2026

Availability

Academic Institutions and Nonprofits only

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pCMV6-AN-DDK_ NLS~K682R_K686R_K69R_K709R (4KR) POLH

Plasmid

#246407

Purpose

Expresses K682R_K686R_K69R_K709R POLH with an N-terminal NLS~FLAG tag in mammalian cells

Depositor

Roger Woodgate

Article

Moreno et al Nucleic Acids Res. 2026 Feb 5;54(4):gkag

Insert

POLH (POLH Human)

Tags

FLAG

Expression

Mammalian

Mutation

POLH K682R_K686R_K69R_K709R. Coding sequence has …

Available Since

Feb. 23, 2026

Availability

Academic Institutions and Nonprofits only

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pCMV6-AN-DDK_WT POLH_ΔGG NEDD8

Plasmid

#221860

Purpose

Expresses FLAG-tagged WT POLH fused to ΔGG NEDD8 in mammalian cells

Depositor

Roger Woodgate

Article

Moreno et al Nucleic Acids Res. 2026 Feb 5;54(4):gkag

Insert

POLH (POLH Human)

Tags

FLAG and ΔGG NEDD8

Expression

Mammalian

Mutation

Coding sequence has been optimised for expression…

Promoter

CMV

Available Since

Feb. 23, 2026

Availability

Academic Institutions and Nonprofits only

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pCMV6-AN-DDK_WT POLH_ΔGG Ubiquitin

Plasmid

#221861

Purpose

Expresses FLAG-tagged WT POLH fused to ΔGG ubiquitin in mammalian cells

Depositor

Roger Woodgate

Article

Moreno et al Nucleic Acids Res. 2026 Feb 5;54(4):gkag

Insert

POLH (POLH Human)

Tags

FLAG and ΔGG Ubiquitin

Expression

Mammalian

Mutation

Coding sequence has been optimised for expression…

Promoter

CMV

Available Since

Feb. 23, 2026

Availability

Academic Institutions and Nonprofits only

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pCMV6-AN-DDK_K682A_K709A POLH

Plasmid

#221864

Purpose

Expresses POLH mutated at K682A and K709A with a FLAG tag in mammalian cells

Depositor

Roger Woodgate

Article

Moreno et al Nucleic Acids Res. 2026 Feb 5;54(4):gkag

Insert

POLH (POLH Human)

Tags

FLAG

Expression

Mammalian

Mutation

POLH K682A + K709A. Coding sequence has been opti…

Promoter

CMV

Available Since

Feb. 23, 2026

Availability

Academic Institutions and Nonprofits only

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pCMV6-AN-DDK_K682A_K686A_K694A_K709A POLH

Plasmid

#221865

Purpose

Expresses POLH mutated at K682A, K686A, K694A and K709A with a FLAG tag in mammalian cells

Depositor

Roger Woodgate

Article

Moreno et al Nucleic Acids Res. 2026 Feb 5;54(4):gkag

Insert

POLH (POLH Human)

Tags

FLAG

Expression

Mammalian

Mutation

POLH K682A, K686A, K694A + K709A. Coding sequence…

Promoter

CMV

Available Since

Feb. 23, 2026

Availability

Academic Institutions and Nonprofits only

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pHR-AL-mCh-Cry2WT-NLS

Plasmid

#221924

Purpose

Expression of CBP autoregulatory loop (AL) fragment fused to optogenetic protein mCh-Cry2WT

Depositor

Daniel Bose

Article

Gelder et al Cell Rep. 2026 Mar 12;45(3):117109. doi:

Insert

CBP AL (aa 1529-1607) (CREBBP Human)

Use

Lentiviral

Tags

mCherry

Expression

Mammalian

Mutation

insertion of the autoregulatory loop (AL) (aa 152…

Available Since

July 1, 2024

Availability

Academic Institutions and Nonprofits only

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AOI-WT-Cas9-sg-mouse Gfi1-exon5 (F3)-GFP

Plasmid

#91884

Purpose

Expresses 3xFLAG-Cas9 and a gRNA targeting mouse Gfi1

Depositor

Martine Roussel

Article

Vo et al Cell Rep. 2017 Mar 21;18(12):2907-2917.

Insert

gRNA targeting Gfi1 (Gfi1 Mouse)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

July 19, 2017

Availability

Academic Institutions and Nonprofits only