We narrowed to 23,750 results for: CRISPR

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pX330-NEAT1pr_v2

Plasmid

#97082

Purpose

Encodes an sgRNA that creates a DSB at the promoter of human NEAT1 gene

Depositor

Archa Fox

Article

Li et al RNA. 2017 Mar 21. pii: rna.059477.116. d

Insert

sgRNA NEAT1pr_v2

Use

CRISPR

Promoter

U6

Available Since

July 28, 2017

Availability

Academic Institutions and Nonprofits only

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pAT9675-CBE

Plasmid

#163007

Purpose

plasmid expressing CBE

Depositor

Ervin Welker

Article

Talas et al Nat Commun. 2021 Nov 3;12(1):6353. doi:

Insert

CBE

Use

CRISPR

Tags

3xFLAG and SV40 NLS

Expression

Mammalian

Promoter

EF1-a

Available Since

Sept. 21, 2021

Availability

Academic Institutions and Nonprofits only

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pCFD3-frame_selector_2

Plasmid

#127555

Purpose

Drosophila expression of frame selector sgRNA 2 that targets CRISPaint site for homology-independent knock-in

Depositor

Norbert Perrimon

Article

Bosch et al Genetics. 2019 Nov 4. pii: genetics.119.

Insert

sgRNA frame 2

Use

CRISPR

Expression

Insect

Promoter

dU6:3

Available Since

July 16, 2019

Availability

Academic Institutions and Nonprofits only

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pCMV-AAAAA-nCas9(D10A)-UGI

Plasmid

#187409

Purpose

DddA AAAAA variant

Depositor

Jin-Soo Kim

Article

Mok et al Nat Commun. 2022 Jul 12;13(1):4038. doi:

Insert

DddA AAAAA

Use

CRISPR

Expression

Mammalian

Available Since

Aug. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pCF221

Plasmid

#121669

Purpose

U6-sgRNA EF1a-mCherry

Depositor

David Savage

Article

Oakes et al Cell. 2019 Jan 10;176(1-2):254-267.e16.

Insert

mCherry

Use

Lentiviral

Promoter

U6

Available Since

March 5, 2019

Availability

Academic Institutions and Nonprofits only

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pMVV205

Plasmid

#215342

Purpose

Recombineering plasmid with constitutively expressed cas9 and the araBAD promoter expressing αβγ on a BBR1 backbone

Depositor

Brian Pfleger

Article

Venkataraman et al ACS Synth Biol. 2023 Dec 15;12(12):3623-

Insert

Cas9

Use

CRISPR and Synthetic Biology

Expression

Bacterial

Promoter

J23105

Available Since

May 21, 2024

Availability

Academic Institutions and Nonprofits only

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pMS13: pTarget(AvCAST)

Plasmid

#168146

Purpose

Target plasmid containing AvPSP1 and attachement site for AvCAST.

Depositor

Feng Zhang

Article

Saito et al Cell. 2021 Apr 29;184(9):2441-2453.e18.

Inserts

PAM for AvCAST + AvPSP1
glmS

Expression

Bacterial

Available Since

June 2, 2021

Availability

Academic Institutions and Nonprofits only

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pRDA_449

Plasmid

#179093

Purpose

nuclease Cas expression

Depositor

John Doench, David Root

Article

Sangree et al Nat Commun. 2022 Mar 14;13(1):1318. doi:

Insert

SpG

Use

CRISPR and Lentiviral

Available Since

Jan. 19, 2022

Availability

Academic Institutions and Nonprofits only

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pX330-NEAT1m_v1

Plasmid

#97083

Purpose

Encodes an sgRNA that creates a DSB immediately downstream the poly(A) signal of human NEAT1_1 isoform

Depositor

Archa Fox

Article

Li et al RNA. 2017 Mar 21. pii: rna.059477.116. d

Insert

sgRNA NEAT1m_v1

Use

CRISPR

Promoter

U6

Available Since

July 28, 2017

Availability

Academic Institutions and Nonprofits only

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pCfB6630

Plasmid

#106160

Purpose

EasyCloneYALI system-based yeast gRNA expression vector carrying a nourseothricin-resistance marker, helps to integrate vector pCfB6371 into Yarrowia lipolytica chromosomal location IntC_3, amp resistance

Depositor

Irina Borodina

Article

Holkenbrink et al Biotechnol J. 2018 Jan 29. doi: 10.1002/

Insert

unknown

Expression

Yeast

Available Since

May 11, 2018

Availability

Academic Institutions and Nonprofits only

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pSN007

Plasmid

#102440

Purpose

For PCR amplification to create paired gRNAs to clone into Golden Gate gRNA expression vectors (e.g. pSB700 Plasmid #64046)

Depositor

Alejandro Chavez, George Church

Article

Nageshwaran et al J Vis Exp. 2018 Oct 2;(140). doi: 10.379

Insert

7SK paired gRNA insert

Use

Synthetic Biology

Expression

Bacterial

Available Since

Aug. 21, 2020

Availability

Academic Institutions and Nonprofits only

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ciCas9_pcDNA5

Plasmid

#100550

Purpose

Expresses ciCas9 in mammalian cells. Can be used to generate Flp-In and Flp-In T-REx stables.

Depositor

Dustin Maly

Article

Rose et al Nat Methods. 2017 Jul 24. doi: 10.1038/n

Insert

ciCas9

Use

CRISPR; Frt, flp-in

Tags

FLAG

Expression

Mammalian

Available Since

Sept. 22, 2017

Availability

Academic Institutions and Nonprofits only

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pU6-cGAS g3 Cas9-T2A-mCherry

Plasmid

#164254

Purpose

Transcription of cGAS guide RNA and Cas9 expression for CRISPR/Cas9 in mammalian cells

Depositor

John Maciejowski

Article

Mohr et al Mol Cell. 2021 Jan 12. pii: S1097-2765(2

Insert

sgcGAS

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

March 30, 2021

Availability

Academic Institutions and Nonprofits only

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pX335-NEAT1_IS_v1

Plasmid

#97085

Purpose

Encodes an sgRNA that creates a SSB near the poly(A) site of human NEAT1_1 isoform.

Depositor

Archa Fox

Article

Li et al RNA. 2017 Mar 21. pii: rna.059477.116. d

Insert

sgRNA NEAT1_IS_v1

Use

CRISPR

Promoter

U6

Available Since

July 28, 2017

Availability

Academic Institutions and Nonprofits only

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pDonor-LoxP

Plasmid

#219436

Purpose

CRISPR-associated transposases from V. cholerae and used for RNA guided cargo insertion in Vibrio natriegens

Depositor

Sheng Yang

Article

Xu et al Biotechnol Bioeng. 2024 Mar;121(3):1163-

Insert

two transposon ends, LoxP-terminator-LoxP gene cassette

Expression

Bacterial

Available Since

June 4, 2024

Availability

Academic Institutions and Nonprofits only

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pMBP-LbCas12a D832A

Plasmid

#113517

Purpose

Expresses His-MBP fused to LbCas12a with D832A mutation to inactivate RuvC nuclease

Depositor

Jennifer Doudna

Article

Chen et al Science. 2018 Apr 27;360(6387):436-439.

Insert

LbCas12a

Tags

MBP

Expression

Bacterial

Mutation

D832A

Promoter

T7

Available Since

July 31, 2018

Availability

Academic Institutions and Nonprofits only

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pX335-NEAT1_IS_v2

Plasmid

#97086

Purpose

Encodes an sgRNA that creates a SSB near the poly(A) site of human NEAT1_1 isoform.

Depositor

Archa Fox

Article

Li et al RNA. 2017 Mar 21. pii: rna.059477.116. d

Insert

sgRNA NEAT1_IS_v2

Use

CRISPR

Promoter

U6

Available Since

July 28, 2017

Availability

Academic Institutions and Nonprofits only

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pU6-p53 gRNA-Cas9-T2A-mCherry

Plasmid

#164256

Purpose

Transcription of p53 guide RNA and Cas9 expression for CRISPR/Cas9 in mammalian cells

Depositor

John Maciejowski

Article

Mohr et al Mol Cell. 2021 Jan 12. pii: S1097-2765(2

Insert

sg p53

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

March 31, 2021

Availability

Academic Institutions and Nonprofits only

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pHAGE-TO-Sp dCas9-mVenusN

Plasmid

#191396

Purpose

SpdCas9 fused to N-terminal part of mVenus

Depositor

Albert Jeltsch

Article

Lungu et al Nat Commun. 2017 Sep 21;8(1):649. doi: 1

Insert

N-terminal part of split mVenus

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 19, 2022

Availability

Academic Institutions and Nonprofits only

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px458_2A_GFP_sgRNA_TRIM25_G1

Plasmid

#127119

Depositor

Thomas Tuschl

Article

Meyer et al Mol Cell. 2020 Mar 19;77(6):1193-1205.e5

Insert

gRNA TRIM25 (TRIM25 Human)

Use

CRISPR

Available Since

July 31, 2019

Availability

Academic Institutions and Nonprofits only

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pUCmini-UGCG-tdTomato

Plasmid

#225879

Purpose

Plasmid donor template for CRISPR/Cas9 mediated UGCG knockout by tdTomato insertion

Depositor

Yohei Sato

Article

Sato et al Biochem Biophys Res Commun. 2024 Sep 18;

Insert

tdTomato

Use

CRISPR

Expression

Mammalian

Available Since

Oct. 10, 2024

Availability

Academic Institutions and Nonprofits only

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px458_2A_GFP_sgRNA_ZNF598

Plasmid

#127121

Depositor

Thomas Tuschl

Article

Meyer et al Mol Cell. 2020 Mar 19;77(6):1193-1205.e5

Insert

gRNA ZNF598 (ZNF598 Human)

Use

CRISPR

Available Since

July 26, 2019

Availability

Academic Institutions and Nonprofits only

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pFS_0271_pET30_pCat-tetR-Term-ptetA-sfGFP-rrnBterm_Fusicatenibacter_saccharivorans_ARRAY2_FaqI

Plasmid

#116955

Purpose

Expresses FsRT-Cas1-Cas2 under pT7lac and sfGFP under ptetA promoter, encodes FsCRISPR array 2, compatible with SENECA acquisition readout

Depositor

Randall Platt

Article

Schmidt et al Nature. 2018 Oct;562(7727):380-385. doi:

Insert

Fusicatenibacter saccharivorans RT-Cas1-Cas2

Expression

Bacterial

Promoter

T7

Available Since

Nov. 2, 2018

Availability

Academic Institutions and Nonprofits only

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pZ147-BvCas12b-sgRNA-scaffold

Plasmid

#122447

Purpose

Expresses sgRNA for BvCas12b in mammalian cells

Depositor

Feng Zhang

Article

Strecker et al Nat Commun. 2019 Jan 22;10(1):212. doi:

Insert

U6-BvCas12b-sgRNA-scaffold, CMV-mCherry

Expression

Mammalian

Available Since

Feb. 21, 2019

Availability

Academic Institutions and Nonprofits only

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M-SPn-VP64

Plasmid

#48674

Purpose

Mammalian SP-VP64 nuclease-null Cas9 activator expression, human optimized

Depositor

George Church

Article

Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n

Insert

Cas9-VP64, nuclease-null

Use

CRISPR

Tags

NLS and VP64

Mutation

nuclease-null

Promoter

CMV

Available Since

Dec. 9, 2013

Availability

Academic Institutions and Nonprofits only
pGH156_MS2-AID-Hygro

Plasmid

#85415

Purpose

lentiviral expression vector for MS2-AID fusion protein with hygromycin B selectable marker

Depositor

Michael Bassik

Article

Hess et al Nat Methods. 2016 Oct 31. doi: 10.1038/n

Insert

MS2-AID and Hygromycin resistance

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Dec. 6, 2016

Availability

Academic Institutions and Nonprofits only

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pU6-TREX1 g2 Cas9-T2A-mCherry

Plasmid

#164251

Purpose

Transcription of TREX1 guide RNA and Cas9 expression for CRISPR/Cas9 in mammalian cells

Depositor

John Maciejowski

Article

Mohr et al Mol Cell. 2021 Jan 12. pii: S1097-2765(2

Insert

sgTREX1

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

March 31, 2021

Availability

Academic Institutions and Nonprofits only

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USP19 sgRNA1

Plasmid

#78585

Purpose

delete USP19 gene in human cell

Depositor

Yihong Ye

Article

Lee et al Nat Cell Biol. 2016 Jul;18(7):765-76. do

Insert

USP19 sgRNA1

Use

CRISPR

Expression

Mammalian

Promoter

CBh

Available Since

June 29, 2016

Availability

Academic Institutions and Nonprofits only
pX330-GFP-SFPQ

Plasmid

#97084

Purpose

Encodes an sgRNA that creates a DSB at the promoter of human SFPQ gene.

Depositor

Archa Fox

Article

Li et al RNA. 2017 Mar 21. pii: rna.059477.116. d

Insert

sgRNA GFP-SFPQ

Use

CRISPR

Promoter

U6

Available Since

July 31, 2017

Availability

Academic Institutions and Nonprofits only

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pTMt_TCS(Q’G)_NLS-HA-dCas9(C)-VP64_T2A_MCP-P65-HSF1

Plasmid

#101102

Purpose

Encodes dCas9(C)-VP64 with SAM components fused to transmembrane tether

Depositor

Tudor Fulga

Article

Baeumler et al Cell Rep. 2017 Sep 12;20(11):2639-2653.

Insert

TMt-dCas9(C)-VP64

Use

CRISPR and Synthetic Biology

Tags

HA and Myc

Expression

Mammalian

Available Since

Nov. 13, 2017

Availability

Academic Institutions and Nonprofits only

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