We narrowed to 13,181 results for: OVA

1. pLz58_polyPro_CsoS2_CTP

   Plasmid

   #140850

   Purpose

   Same as pLz47 but with C-terminal peptide (CTP) instead of N*- peptide

   Depositor

   David Savage

   Article

   Oltrogge et al Nat Struct Mol Biol. 2020 Mar;27(3):281-

   Insert

   polyproline helix with CsoS2 C-terminal peptide
   

   Use

   Tags

   Hexahistidine tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   May 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pLz55_polyPro_random_Npeptide

   Plasmid

   #140846

   Purpose

   Same as pLz47 but with randomized N*-peptide sequence

   Depositor

   David Savage

   Article

   Oltrogge et al Nat Struct Mol Biol. 2020 Mar;27(3):281-

   Insert

   polyproline helix with randomized CsoS2 N-peptide
   

   Use

   Tags

   Hexahistidine tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   May 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pLz56_polyPro_CsoS2_MR_r1

   Plasmid

   #140848

   Purpose

   Same as pLz47 but with MR repeat 1 instead of N*-peptide

   Depositor

   David Savage

   Article

   Oltrogge et al Nat Struct Mol Biol. 2020 Mar;27(3):281-

   Insert

   polyproline helix with CsoS2 MR repeat 1
   

   Use

   Tags

   Hexahistidine tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   May 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pLz57_polyPro_CsoS2_CTD_r8

   Plasmid

   #140849

   Purpose

   Same as pLz47 but with CTD repeat 8 instead of N*-peptide

   Depositor

   David Savage

   Article

   Oltrogge et al Nat Struct Mol Biol. 2020 Mar;27(3):281-

   Insert

   polyproline helix with CsoS2 CTD repeat 8
   

   Use

   Tags

   Hexahistidine tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   May 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pSADuet148

   Plasmid

   #121910

   Purpose

   Charge-introduced NpuDnaBΔ283ΔC39 intein

   Depositor

   Hideo Iwai

   Article

   Beyer et al FEBS J. 2019 Oct 30. doi: 10.1111/febs.1

   Insert

   CI-NpuDnaBΔ290ΔC39 intein
   

   Use

   Tags

   His6-GB1

   Expression

   Bacterial

   Mutation

   Deletion of 290-residue endonuclease domain, I53K…

   Promoter

   T7

   Available since

   Dec. 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pHBDuet259

   Plasmid

   #121911

   Purpose

   NpuDnaBΔ283ΔC39 intein

   Depositor

   Hideo Iwai

   Article

   Beyer et al FEBS J. 2019 Oct 30. doi: 10.1111/febs.1

   Insert

   NpuDnaBΔ283ΔC39 intein
   

   Use

   Tags

   His6-GB1

   Expression

   Bacterial

   Mutation

   Deletion of 283-residue endonuclease domain

   Promoter

   T7

   Available since

   Dec. 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pHBBAD113

   Plasmid

   #121912

   Purpose

   Charge-Introduced NpuDnaBC39 intein

   Depositor

   Hideo Iwai

   Article

   Beyer et al FEBS J. 2019 Oct 30. doi: 10.1111/febs.1

   Insert

   CI-NpuDnaBC39 intein
   

   Use

   Tags

   GB1-His6

   Expression

   Bacterial

   Mutation

   S110K, I111K

   Promoter

   Para

   Available since

   Dec. 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pHBDuet116

   Plasmid

   #121915

   Purpose

   NpuDnaBΔ290ΔC39 intein

   Depositor

   Hideo Iwai

   Article

   Beyer et al FEBS J. 2019 Oct 30. doi: 10.1111/febs.1

   Insert

   Oth-NpuDnaBΔ290ΔC39 intein
   

   Use

   Tags

   His6-GB1

   Expression

   Bacterial

   Mutation

   Deletion of 290-residue endonuclease domain, I53K…

   Promoter

   T7

   Available since

   Dec. 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pAc-Cdc3-Cdc10-SNAP

   Plasmid

   #131161

   Purpose

   Expression of Septin rods

   Depositor

   Nils Johnsson

   Article

   Renz et al BMC Biotechnol. 2013 Jul 26;13:60. doi:

   Insert

   Cdc3 + Cdc10-SNAP
   

   Use

   Bicistronic

   Tags

   Cdc10: SNAP

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Sept. 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pET51b(+)_MBP-nLucCP-65/68

    Plasmid

    #113921

    Purpose

    Plasmid encoding the bacterial expression of the MBP fused NanoLuc circular permuted between the position 65 and 68

    Depositor

    Kai Johnsson

    Article

    Hiblot et al Angew Chem Int Ed Engl. 2017 Nov 13;56(4

    Insert

    MBP-NanoLucCP-65/68
    

    Use

    Luciferase

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Sept. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pET51b(+)_MBP-nLucCP-65/66

    Plasmid

    #113920

    Purpose

    Plasmid encoding the bacterial expression of the MBP fused NanoLuc circular permuted between the position 65 and 66

    Depositor

    Kai Johnsson

    Article

    Hiblot et al Angew Chem Int Ed Engl. 2017 Nov 13;56(4

    Insert

    MBP-NanoLucCP-65/66
    

    Use

    Luciferase

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Sept. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pET51b(+)-LUCIDRS-MTX

    Plasmid

    #113925

    Purpose

    Plasmid encoding the bacterial expression of the red-shifted LUCID semi-synthetic biosensor for methotrexate

    Depositor

    Kai Johnsson

    Article

    Hiblot et al Angew Chem Int Ed Engl. 2017 Nov 13;56(4

    Insert

    LUCID_RS-MTX
    

    Use

    Luciferase

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Sept. 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pKSJ375

    Plasmid

    #103064

    Purpose

    Expresses ImmE1, immunity to colicin E1

    Depositor

    Alan Finkelstein, Karen Jakes

    Article

    Jakes et al J Bacteriol. 2016 Dec 13;199(1). pii: e0

    Insert

    colicin E1 immunity protein (pColE1_26, imm E. coli)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    E1 immunity protein promoter from ColE1

    Available since

    Dec. 18, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pAK22

    Plasmid

    #101741

    Purpose

    encoding two copies of T. thermophilus SecG for expression of SecYEG complex

    Depositor

    Tomoya Tsukazaki

    Article

    Sugano et al PLoS One. 2017 Aug 18;12(8):e0183434. do

    Insert

    Thermus thermophilus HB8 SecG x 2
    

    Use

    Tags

    NONE

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Nov. 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pET-PF2050

    Plasmid

    #100721

    Purpose

    Expression of Pyrococcus furiosus PF2050

    Depositor

    Byung Il Lee

    Article

    Han et al FEBS Lett. 2012 May 7;586(9):1384-8. doi

    Insert

    PF2050
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promotor

    Available since

    Oct. 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. CTL2

    Plasmid

    #92212

    Purpose

    Expresses C-Terminal segment of C. elegans Piezo1 in E. coli

    Depositor

    Douglas Rees

    Article

    Kamajaya et al Structure. 2014 Oct 7;22(10):1520-7. doi

    Insert

    CTL2 of PIEZO1
    

    Use

    Tags

    6x Histidine Tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7 Promoter

    Available since

    Sept. 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pET21a_Msm_RbpA

    Plasmid

    #87928

    Purpose

    Mycobacterium smegmatis RpbA in pET21a (ampR) used to co-express with Msm SigA for crystallization purposes

    Depositor

    Seth Darst

    Article

    Hubin et al Elife. 2017 Jan 9;6. pii: e22520. doi: 1

    Insert

    RbpA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7P

    Available since

    March 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pLacI265-Wasabi

    Plasmid

    #86103

    Purpose

    mWasabi reporter gene gated by temperature-sensitive LacI G265D

    Depositor

    Mikhail Shapiro

    Article

    Piraner et al Nat Chem Biol. 2017 Jan;13(1):75-80. doi

    Inserts

    LacIts
    mWasabi
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    G265D

    Promoter

    T7A1O4/O3 and pLacI

    Available since

    Feb. 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pTetR89-Wasabi

    Plasmid

    #86104

    Purpose

    mWasabi reporter gene gated by temperature-sensitive TetR A89D

    Depositor

    Mikhail Shapiro

    Article

    Piraner et al Nat Chem Biol. 2017 Jan;13(1):75-80. doi

    Inserts

    TetRts
    mWasabi
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    A89D

    Promoter

    ptetA and ptetR

    Available since

    Feb. 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. Lf-MetQ

    Plasmid

    #78305

    Purpose

    Methionine Binding Protein (MetQ) with C-Terminal 6x Histidine Tag

    Depositor

    Douglas Rees

    Article

    Nguyen et al Biol Chem. 2015 Sep;396(9-10):1127-34. d

    Insert

    Methionine Binding Protein (metQ )
    

    Use

    Tags

    6x Histidine Tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pfMDH_K381A

    Plasmid

    #67732

    Purpose

    Expresses K381A mutant of mannitol 2-dehydrogenase from Pseudomonas fluorescens, codon optimized for expression in E. coli

    Depositor

    Justin Siegel

    Article

    Lucas et al Protein Sci. 2015 Jun;24(6):936-45. doi:

    Insert

    Mannitol 2-Dehydrogenase from Pseudomonas fluorescens
    

    Use

    Tags

    6x-His tag

    Expression

    Bacterial

    Mutation

    Changed lysine 381 to alanine

    Promoter

    T7

    Available since

    Sept. 11, 2015

    Availability

    Academic Institutions and Nonprofits only

22. pfMDH_N300D

    Plasmid

    #67728

    Purpose

    Expresses N300D mutant of mannitol 2-dehydrogenase from Pseudomonas fluorescens, codon optimized for expression in E. coli

    Depositor

    Justin Siegel

    Article

    Lucas et al Protein Sci. 2015 Jun;24(6):936-45. doi:

    Insert

    Mannitol 2-Dehydrogenase from Pseudomonas fluorescens
    

    Use

    Tags

    6x-His tag

    Expression

    Bacterial

    Mutation

    Changed asparagine 300 to aspartate

    Promoter

    T7

    Available since

    Sept. 10, 2015

    Availability

    Academic Institutions and Nonprofits only

23. pfMDH_R373A

    Plasmid

    #67731

    Purpose

    Expresses R373A mutant of mannitol 2-dehydrogenase from Pseudomonas fluorescens, codon optimized for expression in E. coli

    Depositor

    Justin Siegel

    Article

    Lucas et al Protein Sci. 2015 Jun;24(6):936-45. doi:

    Insert

    Mannitol 2-Dehydrogenase from Pseudomonas fluorescens
    

    Use

    Tags

    6x-His tag

    Expression

    Bacterial

    Mutation

    Changed arginine 373 to alanine

    Promoter

    T7

    Available since

    Sept. 10, 2015

    Availability

    Academic Institutions and Nonprofits only

24. pfMDH_N300S

    Plasmid

    #67729

    Purpose

    Expresses N300S mutant of mannitol 2-dehydrogenase from Pseudomonas fluorescens, codon optimized for expression in E. coli

    Depositor

    Justin Siegel

    Article

    Lucas et al Protein Sci. 2015 Jun;24(6):936-45. doi:

    Insert

    Mannitol 2-Dehydrogenase from Pseudomonas fluorescens
    

    Use

    Tags

    6x-His tag

    Expression

    Bacterial

    Mutation

    Changed asparagine 300 to serine

    Promoter

    T7

    Available since

    Sept. 9, 2015

    Availability

    Academic Institutions and Nonprofits only

25. PAS531 (TJF074)

    Plasmid

    #65605

    Purpose

    pETDuet-1 Containing FadD V4F, W5L -His

    Depositor

    Pamela Silver

    Article

    Ford et al PeerJ. 2015 Jun 30;3:e1040. doi: 10.7717

    Insert

    FadD
    

    Use

    Tags

    6x His

    Expression

    Bacterial

    Mutation

    V4F, W5L

    Promoter

    T7 lacO

    Available since

    Aug. 10, 2015

    Availability

    Academic Institutions and Nonprofits only

26. PAS509 (TJF032 L448S)

    Plasmid

    #65576

    Purpose

    pETDuet-1 Containing FadD L448S

    Depositor

    Pamela Silver

    Article

    Ford et al PeerJ. 2015 Jun 30;3:e1040. doi: 10.7717

    Insert

    FadD
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    L448S

    Promoter

    T7 lacO

    Available since

    Aug. 10, 2015

    Availability

    Academic Institutions and Nonprofits only

27. pET11a+T214V

    Plasmid

    #66134

    Purpose

    Expresses the T214V mutant of CYP119

    Depositor

    Teruyuki Nagamune

    Article

    Suzuki et al Biotechnol J. 2014 Dec;9(12):1573-81. do

    Insert

    CYP119 (cyp119 )
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    T214V

    Promoter

    T7 promoter

    Available since

    June 29, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. PAS532 (TJF075)

    Plasmid

    #65606

    Purpose

    pETDuet-1 Containing FadD F447S-His

    Depositor

    Pamela Silver

    Article

    Ford et al PeerJ. 2015 Jun 30;3:e1040. doi: 10.7717

    Insert

    FadD
    

    Use

    Tags

    6x His

    Expression

    Bacterial

    Mutation

    F447S

    Promoter

    T7 lacO

    Available since

    June 4, 2015

    Availability

    Academic Institutions and Nonprofits only

29. PAS534 (TJF077)

    Plasmid

    #65608

    Purpose

    pETDuet-1 Containing FadD D372G-His

    Depositor

    Pamela Silver

    Article

    Ford et al PeerJ. 2015 Jun 30;3:e1040. doi: 10.7717

    Insert

    FadD
    

    Use

    Tags

    6x His

    Expression

    Bacterial

    Mutation

    D372G

    Promoter

    T7 lacO

    Available since

    June 3, 2015

    Availability

    Academic Institutions and Nonprofits only

30. PAS535 (TJF078)

    Plasmid

    #65609

    Purpose

    pETDuet-1 Containing FadD Y9H-His

    Depositor

    Pamela Silver

    Article

    Ford et al PeerJ. 2015 Jun 30;3:e1040. doi: 10.7717

    Insert

    FadD
    

    Use

    Tags

    6x His

    Expression

    Bacterial

    Mutation

    Y9H

    Promoter

    T7 lacO

    Available since

    June 3, 2015

    Availability

    Academic Institutions and Nonprofits only