165,688 results

1. pLIB-HIS-TEV-Cyclin-B

   Plasmid

   #177012

   Purpose

   Generate baculovirus for insect cell expression of Cyclin-B with N-terminal Polyhistidine-TEV

   Depositor

   Andrea Musacchio

   Article

   Huis In 't Veld et al Protein Sci. 2021 Nov 17. doi: 10.1002/p

   Insert

   Cyclin B1 (CCNB1 Human)
   

   Tags

   Polyhistidine-TEV

   Expression

   Insect

   Mutation

   codon-optimised for insect cell expression

   Available Since

   Nov. 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pRSFDuet-1 ElonginB, ElonginC

   Plasmid

   #230999

   Purpose

   Dual expression vector containing mouse ElonginB and ElonginC for E.coli protein purification

   Depositor

   Michael Rape

   Article

   Manford et al Cell. 2020 Oct 1;183(1):46-61.e21. doi:

   Inserts

   Elongin B (Elob Mouse)
   Elongin C (Eloc Mouse)
   

   Expression

   Bacterial

   Available Since

   Jan. 22, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pKLV2-EF1a-BsdCas9-W

   Plasmid

   #67978

   Purpose

   Lentiviral vector expressing Cas9 fused with the Blasticidin resistant gene at the N-terminus

   Depositor

   Kosuke Yusa

   Article

   Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

   Insert

   EF1a-Cas9 cassette, WPRE
   

   Use

   CRISPR and Lentiviral

   Tags

   Cas9 fused with Flag-tag and a NLS at the N termi…

   Promoter

   EF1a

   Available Since

   Sept. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV-Syn_CapChR2_mScarlet

   Plasmid

   #188039

   Purpose

   Expresses CapChR2-mScarlet in mammalian neurons

   Depositor

   Peter Hegemann

   Article

   Fernandez Lahore et al Nat Commun. 2022 Dec 21;13(1):7844. doi:

   Insert

   CapChR2-mScarlet
   

   Use

   AAV

   Tags

   mScarlet

   Mutation

   S43D, L112C, T139C and N238E mutations in CoChR

   Promoter

   hSyn

   Available Since

   Dec. 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. HLA_A2402

   Plasmid

   #165010

   Purpose

   Expression of HLA-A*24:02 cDNA

   Depositor

   Matthew Meyerson

   Article

   Lee et al Sci Immunol. 2021 Jan 22;6(55). pii: 6/5

   Insert

   HLA-A*24:02 (HLA-A Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   March 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV-GFAP-hM3D(Gq)-mCherry

   Plasmid

   #50478

   Purpose

   Gq-coupled hM3D DREADD fused with mCherry under the control of GFAP promoter

   Depositor

   Bryan Roth

   Has Service

   AAV5

   Insert

   hM3D(Gq)-mCherry
   

   Use

   AAV

   Tags

   mCherry

   Mutation

   See supplemental documents for DREADD mutations

   Promoter

   GFAP

   Available Since

   April 17, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCDH-EF1-IRES-BSD

   Plasmid

   #209751

   Purpose

   Lentiviral transfer plasmid to express the blasticidin resistance gene

   Depositor

   Andrei Thomas-Tikhonenko

   Article

   Ang et al Blood. 2023 Sep 8:blood.2023020400. doi:

   Type

   Empty backbone

   Use

   Lentiviral

   Available Since

   Nov. 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCDNA3.1(+)-NEMOc

   Plasmid

   #189933

   Purpose

   Mammalian expression of calcium sensor with ultra-high dynamics and sensitivity

   Depositor

   Yubin Zhou

   Article

   Li et al Nat Methods. 2023 Jun;20(6):918-924. doi

   Insert

   NEMOc
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. PE7_AAVS1_knockin_HDR_donor

   Plasmid

   #223001

   Purpose

   HDR donor plasmid for PE7 knockin at AAVS1 locus. Left_homology_arm-splicing_acceptor-T2A-NeoR-bGHpA-pEF1α-PE7-IRES2-EGFP-WPRE-βglobinpA-right_homology_arm

   Depositor

   Brittany Adamson

   Article

   Yan et al Nature. 2024 Apr 3. doi: 10.1038/s41586-

   Insert

   PE7
   

   Use

   Hdr donor

   Tags

   SV40 bpNLS and c-Myc NLS

   Expression

   Mammalian

   Promoter

   EF1α

   Available Since

   Aug. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3.1+-mutTornado-split-nLuc

    Plasmid

    #212707

    Purpose

    Tornado split nLuc with a mutated 3' ribozyme so that circularization cannot occur.

    Depositor

    Samie Jaffrey

    Article

    Unti et al Cell Chem Biol. 2023 Oct 13:S2451-9456(2

    Insert

    mutTornado-CVB3-split-nLuc
    

    Mutation

    Deleted the partial 3'ribozyme sequence in T…

    Available Since

    Feb. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCAG_PCP-T5Exo

    Plasmid

    #214736

    Purpose

    encodes T5 exonuclease with a PP7 coat protein fused to the N terminus

    Depositor

    Gil Westmeyer

    Article

    Truong et al Nat Methods. 2024 Feb 1. doi: 10.1038/s4

    Insert

    CAG_PCP-T5Exo_bGH
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Mammalian

    Available Since

    March 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMal-Abl-PRM 5R

    Plasmid

    #112088

    Purpose

    Bacterial expression plasmid containing His and MBP tags for 5 PRM motif repeats of Human Abl.

    Depositor

    Michael Rosen

    Article

    Li et al Nature. 2012 Mar 7;483(7389):336-40. doi

    Insert

    PRM-5R (ABL1 Human)
    

    Tags

    His-6 and MBP

    Expression

    Bacterial

    Mutation

    construct contains only the PRM motif of Abl1

    Promoter

    Lac

    Available Since

    Feb. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pSems-leader-mXFPm-IFNGR1(18-489)

    Plasmid

    #192786

    Purpose

    Expression of mXFPm-tagged IFNGR1 at the plasma membrane, e.g. for single molecule fluorescence microscopy

    Depositor

    Jacob Piehler

    Article

    Sotolongo Bellon et al Cell Rep Methods. 2022 Feb 4;2(2):100165

    Insert

    IFNGR1 (IFNGR1 Human)
    

    Tags

    Ig k-chain leader sequence and mXFPm

    Expression

    Mammalian

    Mutation

    mXFPm: tryptophan 66 to phenylalanine, gluatmic a…

    Promoter

    CMV

    Available Since

    Dec. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. EphA2-YFP

    Plasmid

    #108852

    Purpose

    Encodes for human EphA2 fluorescently labeled with eYFP on the C-Terminus via a 15 amino acid (GGS)5 flexible linker

    Depositor

    Kalina Hristova

    Article

    Singh et al J Biol Chem. 2015 Nov 6;290(45):27271-9.

    Insert

    EPHA2 (EPHA2 Human)
    

    Tags

    Labeled with eYFP on the C-Terminus via a 15 amin…

    Expression

    Mammalian

    Available Since

    April 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. TRUPATH Triple GaZ

    Plasmid

    #196053

    Purpose

    Encodes a G alpha subunit (GNAZ) with RLuc8, a G gamma subunit (GNG1) with GFP2 and a G beta subunit (GNB3) as optimal components of a BRET2 biosensor for studying heterotrimeric G proteins

    Depositor

    Justin English

    Article

    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

    Inserts

    GAlphaz-RLuc8 (GNAZ Human)
    GGamma1-GFP2 (GNGT1 Human)
    GBeta3 (GNB3 Human)
    

    Use

    Luciferase

    Tags

    GFP2 and GSAG linker

    Expression

    Mammalian

    Mutation

    RLuc8 and flanking SGGGGS linkers have been inser…

    Promoter

    CMV

    Available Since

    March 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pNFkB:eGFP

    Plasmid

    #44922

    Depositor

    John Rawls

    Article

    Kanther et al Gastroenterology. 2011 Jul;141(1):197-20

    Insert

    EGFP
    

    Available Since

    Sept. 3, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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17. pSLQ4428 pHR (EF1a-mCherry-P2A-RfxCas13d-2xNLS-ecDHFR-3xFLAG-WPRE)

    Plasmid

    #214885

    Purpose

    Lentiviral vector encoding TMP-regulatable RfxCas13d-DD fusion

    Depositor

    Stanley Qi

    Article

    Tieu et al Cell. 2024 Feb 29;187(5):1278-1295.e20.

    Insert

    EF1a-mCherry-P2A-RfxCas13d-2xNLS-ecDHFR-3xFLAG-WPRE
    

    Use

    CRISPR and Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    April 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pF146 pSOD1WTAcGFP1

    Plasmid

    #26407

    Depositor

    Elizabeth Fisher

    Article

    Stevens et al PLoS One. 2010 . 5(3):e9541.

    Insert

    SOD1 (SOD1 Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Available Since

    Nov. 8, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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19. EGFP-Bak

    Plasmid

    #32564

    Depositor

    Richard Youle

    Article

    Nechushtan et al J Cell Biol. 2001 Jun 11. 153(6):1265-76

    Insert

    human Bak (BAK1 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 29, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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20. tsPurple chromoprotein

    Plasmid

    #117848

    Purpose

    BioBrick pSB1C3 plasmid that constitutively overexpresses tsPurple chromoprotein in E. coli

    Depositor

    Anthony Forster

    Article

    Liljeruhm et al J Biol Eng. 2018 May 10;12:8. doi: 10.11

    Insert

    promoter, RBS, tsPurple
    

    Use

    Synthetic Biology; Escherichia coli

    Mutation

    BioBrick sites removed

    Available Since

    Aug. 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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21. pLV-ER GFP

    Plasmid

    #80069

    Purpose

    To express and target GFP into the endoplasmic reticulum using lentivirus

    Depositor

    Pantelis Tsoulfas

    Insert

    ER GFP (SEC61B Human)
    

    Use

    Lentiviral

    Tags

    GFP derived from Aequorea coerulescens on the NH2…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 27, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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22. pGWB533

    Plasmid

    #74872

    Purpose

    Gateway cloning compatible binary vector for C-terminal fusion with GUS (no promoter).

    Depositor

    Tsuyoshi Nakagawa

    Article

    Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

    Type

    Empty backbone

    Expression

    Plant

    Available Since

    Oct. 18, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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23. pLenti Lifeact-mRuby2 PuroR

    Plasmid

    #84386

    Purpose

    Lentiviral expression of mRuby2-tagged Lifeact with Puromycin selection in cells including neurons

    Depositor

    Ghassan Mouneimne

    Insert

    Lifeact
    

    Use

    Lentiviral

    Tags

    mRuby2

    Promoter

    CMV immediate early

    Available Since

    Jan. 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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24. pcDNA3.1 FLAG-YY1

    Plasmid

    #104396

    Purpose

    FLAG-YY1

    Depositor

    Richard Young

    Article

    Weintraub et al Cell. 2017 Dec 14;171(7):1573-1588.e28.

    Insert

    YY1
    

    Tags

    FLAG

    Available Since

    Feb. 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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25. pCONR221

    Plasmid

    #216146

    Purpose

    Gateway Donor Vector with attP1 and attP2 sites modified from PDONR221 and Ampicillin resistance

    Depositor

    Imran Siddiqi

    Article

    Sivakumar et al Physiol Mol Biol Plants. 2024 Jul;30(7):

    Type

    Empty backbone

    Use

    Cloning backbone

    Available Since

    June 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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26. pMSGV-mCD105scFv-myc-CD28HTMcostim-T2A-Thy1.1

    Plasmid

    #193942

    Purpose

    a fully murine CAR targeting CD105 (endoglin), a component of the TGFβ receptor expressed on the surface of certain solid tumors and acute leukemias.

    Depositor

    Greg Delgoffe

    Article

    Lontos et al Oncoimmunology. 2022 Oct 18;11(1):213122

    Insert

    heavy- and light-chain regions of the MJ7/18 hybridoma and constructed a single chain variable fragment (scFv)
    

    Use

    Retroviral

    Available Since

    May 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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27. HiFi Cas9

    Plasmid

    #188490

    Purpose

    Expresses FLAG tagged HiFi Cas9

    Depositor

    Isaac Hilton

    Article

    Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

    Insert

    Cas9
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Mutation

    R691A

    Promoter

    EF1a

    Available Since

    Sept. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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28. pWPI-IRES-Puro-Ak-ACE2-TMPRSS2

    Plasmid

    #154987

    Purpose

    Lentiviral vector expressing Human ACE2 and TMPRSS2

    Depositor

    Sonja Best

    Insert

    ACE2 and TMPRSS2 (ACE2, TMPRSS2 Human)
    

    Use

    Lentiviral

    Available Since

    Sept. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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29. 1136- Desmoplakin-GFP

    Plasmid

    #32227

    Depositor

    Kathleen Green

    Article

    Godsel et al J Cell Biol. 2005 Dec 19;171(6):1045-59.

    Insert

    Desmoplakin (DSP Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 8, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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30. PRS_8-ChrimsonR-tdTomato

    Plasmid

    #192587

    Purpose

    AAV-vector for optogenetic activation of noradrenergic neurons

    Depositor

    Simon Wiegert

    Article

    Wissing et al PLoS Biol. 2025 Jul 7;23(7):e3003228. do

    Insert

    ChrimsonR
    

    Use

    AAV

    Tags

    tdTomato

    Expression

    Mammalian

    Promoter

    PRSX8

    Available Since

    Dec. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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