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Showing: 11701 - 11730 of 158095 results

  2. pLKO-FOXM1-shRNA-81

    Plasmid
    #89495
    Purpose
    Lentiviral vector with constitutive expression of shRNA targeting human FOXM1.
    Depositor
    Adam Karpf
    Insert
    FOXM1 (FOXM1 Human)
    Use
    Lentiviral and RNAi
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6
    Available since
    June 1, 2017
    Availability
    Academic Institutions and Nonprofits only
    89495-plasmid-map-sequence-id-180322

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  5. N-Terminal Split Cas9 with GyrA intein

    Plasmid
    #58693
    Purpose
    Expresses truncated N-terminal SpCas9 domain fused to a GyrA intein, flanked by ITRs for AAV packaging. Combine with C-Terminal Split Cas9 Gyra Intein for full length SpCas9 production
    Depositor
    Gang Bao
    Article
    Fine et al Sci Rep. 2015 Jul 1;5:10777. doi: 10.103
    Insert
    Humanized N-Terminal S. pyogenes Cas9 with GyrA Nsplit Intein
    Use
    AAV and CRISPR
    Tags
    3xFlag, GyrA Nsplit Intein, and NLS
    Expression
    Mammalian
    Mutation
    Promoter
    CBh
    Available since
    July 6, 2015
    Availability
    Academic Institutions and Nonprofits only
    58693-plasmid-map-sequence-id-90684

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  7. AY27_pU6.gRNA.CLYBL

    Plasmid
    #199238
    Purpose
    Expression construct encoding a S. pyogenes guide RNA targeting the human CLYBL safe harbor locus
    Depositor
    Manuel Goncalves
    Article
    Wang et al Nucleic Acids Res. 2023 Mar 17:gkad165.
    Insert
    S. pyogenes gRNA spacer
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    RNA polymerase III promoter for human U6 snRNA
    Available since
    April 28, 2023
    Availability
    Academic Institutions and Nonprofits only
    199238-plasmid-map-sequence-id-391387

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  13. p-dCas9-G9a-Hygro

    Plasmid
    #104408
    Purpose
    transient expression of dCas9-G9a fusion protein
    Depositor
    Stephan Beck
    Insert
    G9a (EHMT2 Human)
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV promoter
    Available since
    April 2, 2020
    Availability
    Academic Institutions and Nonprofits only
    104408-plasmid-map-sequence-id-204469

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  14. pLPC-JAG1-mVenus

    Plasmid
    #171170
    Purpose
    Retroviral vector for CMV promoter driven expression of JAG1 (Notch pathway) fused to mVenus fluorescent protein (to be used in conjunction with Phoenix packaging cells).
    Depositor
    Masashi Narita
    Article
    Hoare et al Nat Cell Biol. 2016 Sep;18(9):979-92. do
    Insert
    JAG1 (JAG1 Human)
    Use
    Retroviral
    Tags
    mVenus
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    April 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    171170-plasmid-map-sequence-id-339658

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  15. qTAG-N-Solo-mStayGold-TJP1

    Plasmid
    #227300
    Purpose
    Donor template for mStayGold insertion into the N-terminus of the TJP1 locus. For tight junction visualization. To be co-transfected with sgRNA plasmid px330-TJP1 (Addgene #227299)
    Depositor
    Laurence Pelletier
    Article
    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318
    Insert
    TJP1 Homology Arms flanking a mStayGold Tag (TJP1 Human)
    Use
    CRISPR; Donor template
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Promoterless
    Available since
    Nov. 25, 2024
    Availability
    Academic Institutions and Nonprofits only
    227300-plasmid-map-sequence-id-451538

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  22. TFORF1035

    Plasmid
    #143746
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    WT1 (WT1 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    Sept. 14, 2023
    Availability
    Academic Institutions and Nonprofits only
    143746-plasmid-map-sequence-id-407847

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  23. TFORF2903

    Plasmid
    #143659
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    DACH1 (DACH1 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    July 11, 2023
    Availability
    Academic Institutions and Nonprofits only
    143659-plasmid-map-sequence-id-400444

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  24. TFORF1038

    Plasmid
    #144075
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    WT1 (WT1 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    June 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    144075-plasmid-map-sequence-id-396215

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  25. pOPARA1

    Plasmid
    #200416
    Purpose
    Golden Gate assembly of expression constructs containing pBAD promoter (carbenicillin resistance)
    Depositor
    Kamil Witek
    Article
    Lesley et al bioRxiv
    Type
    Empty backbone
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    pBAD
    Available since
    May 24, 2023
    Availability
    Academic Institutions and Nonprofits only
    200416-plasmid-map-sequence-id-394143

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  28. A1-LCD+7R+12D

    Plasmid
    #178678
    Purpose
    Bacterial expression of N-terminally 6His tagged A1-LCD with seven Arg residues added, twelve Glu residues added (LCD: low-complexity domain, aa 186-320)
    Depositor
    Tanja Mittag
    Article
    Bremer et al Nat Chem. 2022 Feb;14(2):196-207. doi: 1
    Insert
    A1-LCD+7R+12D (HNRNPA1 Human)
    Use
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    seven Arg residues added, twelve Glu residues add…
    Promoter
    Available since
    March 17, 2022
    Availability
    Academic Institutions and Nonprofits only
    178678-plasmid-map-sequence-id-357023

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  30. pAAV-CaMKIIa-HA-hM3D(Gq)-IRES-mCitrine

    Plasmid
    #50466
    Purpose
    Gq-coupled hM3D-IRES-mCitrine under the control of CaMKIIa promoter
    Depositor
    Bryan Roth
    Insert
    hM3D(Gq)-IRES-mCitrine (CHRM3 Human)
    Use
    AAV
    Tags
    HA
    Expression
    Mutation
    See supplemental documents for DREADD mutations
    Promoter
    CaMKIIa
    Available since
    April 16, 2014
    Availability
    Academic Institutions and Nonprofits only
    50466-plasmid-map-sequence-id-305068

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Showing: 11701 - 11730 of 158095 results