We narrowed to 88,835 results for: Mal;

1. pCVL.SFFV.Kozak.HA.NLS.Y2AniK227M.IRES.BFP

   Plasmid

   #45579

   Purpose

   Expresses K227M nickase variant of I-Ani I nuclease in mammalian cells with BFP tracker

   Depositor

   Andrew Scharenberg

   Article

   Kuhar et al Nucleic Acids Res. 2013 Oct 10.

   Insert

   coY2 Ani K227M
   

   Use

   Lentiviral

   Tags

   HA, IRES BFP, and NLS

   Expression

   Mammalian

   Mutation

   co indicates codon optimization for mammalian exp…

   Promoter

   SFFV

   Available Since

   Nov. 4, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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2. pPE3-pPuro

   Plasmid

   #215722

   Purpose

   Cloning vector for Uni-Vector prime editing system

   Depositor

   Kou-Juey Wu

   Article

   Huang et al Biotechnol J. 2024 Feb;19(2):e2300353. d

   Insert

   PE2
   

   Use

   Unspecified

   Promoter

   EFS promoter

   Available Since

   Dec. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pU6T7

   Plasmid

   #71462

   Purpose

   Expresses gRNA from hybrid human U6/T7 promoter for both cellular expression and in vitro transcription from same promoter (2 Gs at initiation)

   Depositor

   Willie Mark

   Article

   Romanienko et al PLoS One. 2016 Feb 5;11(2):e0148362. doi

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Bacterial and Mammalian

   Promoter

   human U6/phage T7

   Available Since

   Feb. 9, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCAG-T3-CjCAS9-pA

   Plasmid

   #131543

   Purpose

   Expresses Campylobacter jejuni-derived Cas9 nuclease in mammalian cells. Used to modify genome of mouse embroys

   Depositor

   Wataru Fujii

   Article

   Fujii et al Int J Mol Sci. 2017 Oct 31;18(11). pii:

   Insert

   CjCas9
   

   Use

   CRISPR

   Tags

   FLAG-SV40NLS, SV40NLS, and polyA

   Expression

   Mammalian

   Mutation

   Human codon-optimized

   Promoter

   CAG, T3

   Available Since

   Nov. 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pEGFP-C1-hUVRAG

   Plasmid

   #24296

   Depositor

   Noboru Mizushima

   Article

   Itakura et al Mol Biol Cell. 2008 Dec . 19(12):5360-72

   Insert

   UV irradiation resistance-associated gene (UVRAG Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Available Since

   June 24, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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6. xCas9(3.6)-BE4

   Plasmid

   #108386

   Purpose

   Mammalian expression vector for xCas9(3.6)-BE4

   Depositor

   David Liu

   Article

   Hu et al Nature. 2018 Feb 28. pii: nature26155. d

   Insert

   xCas9(3.6)-BE4
   

   Tags

   NLS

   Expression

   Mammalian

   Mutation

   E108G, S217A, A262T, S409I, E480K, E543D, M694I, …

   Available Since

   March 28, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. AAV-sCAG-eGFP-ACAP1 F280E

   Plasmid

   #203801

   Purpose

   AAV vector plasmid expressing human ACAP1 mutation F280E fused to eGFP under a short variant of the CAG (sCAG) promoter

   Depositor

   Richard Eva, Bart Nieuwenhuis

   Insert

   ACAP1 (ACAP1 Human)
   

   Use

   AAV

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   F280E

   Available Since

   Sept. 13, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pTEV5_ RpMatB_2

   Plasmid

   #78206

   Purpose

   Expression of double-cysteine variant of Malonyl-coenzyme A synthetase, RpMatB, as scaffold for ATP biosensor - tighter binding

   Depositor

   Martin Webb

   Article

   Vancraenenbroeck et al PLoS One. 2017 Jun 21;12(6):e0179547. do

   Insert

   matB (His6 C106A S170A R286C Q457C K488A)
   

   Tags

   His6

   Expression

   Bacterial

   Mutation

   C106A S170A R286C Q457C K488A

   Promoter

   T7

   Available Since

   July 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. PGK+ plasmid with onboard mCherry cassette

   Plasmid

   #169745

   Purpose

   PGK+ plasmid that also encodes separate mCherry cassette. mCherry is used to monitor plasmid dosage.

   Depositor

   Francois St-Pierre

   Article

   Yang et al Nat Commun. 2021 Jul 5;12(1):4132. doi:

   Insert

   eGFP
   

   Use

   Synthetic Biology

   Expression

   Mammalian

   Promoter

   PGK-tetO2

   Available Since

   June 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3.1-StrepHA-SUMO2(Q87R)

    Plasmid

    #66868

    Purpose

    Mammalian expression of SUMO2(Q87R) with a strep and an HA tag

    Depositor

    Ying Liu

    Article

    Bursomanno et al J Proteome Res. 2015 Apr 3;14(4):1687-99

    Insert

    SUMO2 (SUMO2 Human)
    

    Tags

    HA and Strep

    Expression

    Mammalian

    Mutation

    Q87R

    Promoter

    CMV

    Available Since

    June 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCDH-CRY2-mCh-superPLDx10

    Plasmid

    #188986

    Purpose

    A lentiviral plasmid encoding CRY2-mCh-superPLDx10 (an engineered PLD mutant with 10 times higher activity than the wild-type)

    Depositor

    Jeremy Baskin

    Article

    Tei et al Nat Chem. 2023 May 22. doi: 10.1038/s415

    Insert

    PLD
    

    Tags

    CRY2 and mCherry

    Expression

    Mammalian

    Mutation

    F163L, K249R, G328S, G381V, G429D, D480G

    Promoter

    CMV

    Available Since

    May 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. Stat1 alpha Y701F pRc/CMV

    Plasmid

    #8701

    Depositor

    Jim Darnell

    Article

    Wen et al Cell 1995 Jul 28;82(2):241-50.

    Insert

    STAT1 alpha Y701F (STAT1 Human)
    

    Expression

    Mammalian

    Mutation

    Y701F

    Available Since

    Feb. 13, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCD5-D/bovine/Nebraska/9-5/2012-HEFwtED-GCN4-sfGFP-ST

    Plasmid

    #175019

    Purpose

    Expresses influenza D virus trimeric hemagglutinin esterase fusion protein fused to a sfGFP

    Depositor

    Robert de Vries

    Article

    Nemanichvili et al Viruses. 2021 Mar 31;13(4). pii: v130405

    Insert

    D/bovine/Nebraska/9-5/2012
    

    Tags

    GCN4-TEV-sfGFP-TwinStrep

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pSRalpha-MSV-mouse Cdk4wt-Tk-neo

    Plasmid

    #115525

    Purpose

    Mammalian expression construct

    Depositor

    Martine Roussel

    Article

    Matsushime et al Cell. 1992 Oct 16;71(2):323-34.

    Insert

    Mouse Cdk4 (Cdk4 Mouse)
    

    Use

    Retroviral

    Available Since

    Oct. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

15. xCas9(3.6)-BE3

    Plasmid

    #108385

    Purpose

    Mammalian expression vector for xCas9(3.6)-BE3

    Depositor

    David Liu

    Article

    Hu et al Nature. 2018 Feb 28. pii: nature26155. d

    Insert

    xCas9(3.6)-BE3
    

    Tags

    NLS

    Expression

    Mammalian

    Mutation

    E108G, S217A, A262T, S409I, E480K, E543D, M694I, …

    Available Since

    March 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCI-neo-HA-hAtg14

    Plasmid

    #24294

    Depositor

    Noboru Mizushima

    Article

    Itakura et al Mol Biol Cell. 2008 Dec . 19(12):5360-72

    Insert

    autophagy related genes 14 (ATG14 Human)
    

    Tags

    3xHA

    Expression

    Mammalian

    Available Since

    Sept. 15, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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17. pMito-miRFP718nano

    Plasmid

    #195754

    Purpose

    Mitochondria labelled with near-infrared fluorescent protein miRFP718nano

    Depositor

    Vladislav Verkhusha

    Article

    Oliinyk et al Nat Methods. 2022 Dec 1. doi: 10.1038/s4

    Insert

    Human cytochrome C oxidase subunit VIII-miRFP718nano
    

    Tags

    miRFP718nano

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. His-NavMs

    Plasmid

    #100004

    Purpose

    Electrophysiology in mammalian cells: Mammalian expression of voltage gated sodium channel and GFP

    Depositor

    Paul DeCaen

    Article

    Sula et al Nat Commun. 2017 Feb 16;8:14205. doi: 10

    Insert

    His-NavMs, Voltage gated sodium channel
    

    Tags

    Hexa-His tag followed by a thrombin cleavage site

    Expression

    Mammalian

    Mutation

    C-terminus from a codon-optimized synthetic gene,…

    Promoter

    CMV

    Available Since

    Aug. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pFX-EGFP-Rab11

    Plasmid

    #174460

    Purpose

    Express iRFP with recycling endosome-targeting sequence in mammalian cells

    Depositor

    Yusuke Ohba

    Article

    Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

    Insert

    EGFP with recycling endosome-targeting sequence
    

    Tags

    small GTPase (Rab11)

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pH2B-miRFP718nano

    Plasmid

    #195750

    Purpose

    Histone 2B labelled with near-infrared fluorescent protein miRFP718nano

    Depositor

    Vladislav Verkhusha

    Article

    Oliinyk et al Nat Methods. 2022 Dec 1. doi: 10.1038/s4

    Insert

    H2B-miRFP718nano
    

    Tags

    miRFP718nano

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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