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pVMG0146_Cq-U6-1_2xBbsI-gRNA

Plasmid

#169238

Purpose

Expression of gRNA in Culex quinquefasciatus or other mosquitoes

Depositor

Valentino Gantz

Article

Feng et al Nat Commun. 2021 May 20;12(1):2960. doi:

Insert

Cq-U6-1_2xBbsI-gRNA

Use

CRISPR

Expression

Insect

Promoter

CPIJ039653

Available Since

May 26, 2021

Availability

Academic Institutions and Nonprofits only

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pVMG0149_Cq-U6-6_2xBbsI-gRNA

Plasmid

#169323

Purpose

Expression of gRNA in Culex quinquefasciatus or other mosquitoes

Depositor

Valentino Gantz

Article

Feng et al Nat Commun. 2021 May 20;12(1):2960. doi:

Insert

pVMG0149_Cq-U6-6_2xBbsI-gRNA

Use

CRISPR

Expression

Insect

Promoter

CPIJ039596

Available Since

May 21, 2021

Availability

Academic Institutions and Nonprofits only

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pCASP-SptP120-K27-HilA

Plasmid

#153328

Purpose

Triggers secretion of SptP120 fused to the K27 DARPin from Salmonella upon induction with arabinose

Depositor

Josef Penninger

Article

Chabloz et al Commun Biol. 2020 Jul 3;3(1):342. doi: 1

Insert

SptP120-K27 DARPin

Use

Salmonella expression

Available Since

July 21, 2020

Availability

Academic Institutions and Nonprofits only

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pC-KIT2

Plasmid

#118984

Purpose

Mutational dissection of the G rich region of the c-kit promoter

Depositor

Bjorn Hogberg

Article

Ducani et al J Am Chem Soc. 2019 Jul 3;141(26):10205-

Insert

c-kit proto-oncogene {promoter} (KIT Human)

Expression

Mammalian

Mutation

mutK2-SP-K1

Available Since

June 10, 2019

Availability

Academic Institutions and Nonprofits only

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pC-KIT4

Plasmid

#118986

Purpose

Mutational dissection of the G rich region of the c-kit promoter

Depositor

Bjorn Hogberg

Article

Ducani et al J Am Chem Soc. 2019 Jul 3;141(26):10205-

Insert

c-kit proto-oncogene {promoter} (KIT Human)

Expression

Mammalian

Mutation

K2-SP-mutK1

Available Since

June 10, 2019

Availability

Academic Institutions and Nonprofits only

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pC-KIT6

Plasmid

#118988

Purpose

Mutational dissection of the G rich region of the c-kit promoter

Depositor

Bjorn Hogberg

Article

Ducani et al J Am Chem Soc. 2019 Jul 3;141(26):10205-

Insert

c-kit proto-oncogene {promoter} (KIT Human)

Expression

Mammalian

Mutation

mutK2-SP-mutK1

Available Since

June 10, 2019

Availability

Academic Institutions and Nonprofits only

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GFP-CmTMC1truncated

Plasmid

#154908

Purpose

For the expression and purification of the truncated TMC1 protein from Chelonia mydas. The protease cleavage site was replaced with the HRV 3C site.

Depositor

Motoyuki Hattori

Article

Jia et al Neuron. 2020 Jan 22;105(2):310-321.e3. d

Insert

GFP-CmTMC1truncated

Tags

8histidine-EGFP

Expression

Insect

Mutation

Truncations of the residues 1-243 and 843-969

Available Since

Sept. 10, 2020

Availability

Academic Institutions and Nonprofits only

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GFP-MuTMC2truncated

Plasmid

#154909

Purpose

For the expression and purification of the truncated TMC2 protein from Melopsittacus undulates. The protease cleavage site was replaced with the HRV 3C site.

Depositor

Motoyuki Hattori

Article

Jia et al Neuron. 2020 Jan 22;105(2):310-321.e3. d

Insert

GFP-MuTMC2truncated

Tags

8histidine-EGFP

Expression

Insect

Mutation

Truncations of the residues 1-179 and 691-716

Available Since

July 27, 2020

Availability

Academic Institutions and Nonprofits only

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pOEM-GED-mCherry

Plasmid

#102781

Purpose

Baculovirus rescue vector, VSVGED pseudotype, mCherry

Depositor

Elly Tanaka

Article

Oliveira et al Dev Biol. 2018 Jan 15;433(2):262-275. do

Insert

mCherry

Use

Baculoviral rescue shuttle vector

Expression

Insect and Mammalian

Promoter

CMV

Available Since

Dec. 21, 2017

Availability

Academic Institutions and Nonprofits only

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pUASP dsRed Rab5 DN

Plasmid

#44823

Depositor

Matthew Scott

Article

Zhang et al Genetics. 2007 Jun;176(2):1307-22. Epub

Insert

Rab5 (Rab5 Fly)

Use

Drosophila

Tags

dsRed

Expression

Insect

Mutation

S43N (dominant negative)

Available Since

April 18, 2013

Availability

Academic Institutions and Nonprofits only
pTS2381-Tier1-PhCMV-Igk_TM

Plasmid

#169487

Purpose

Tier-1 vector encoding PhCMV-driven transmembrane domain from Junctophilin-1 (TM) for membrane localization of fusion proteins attached to a secretion signal Igk (PhCMV-IgkSS-TM-pA).

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

PCMV-driven membrane localization

Expression

Mammalian

Promoter

PhCMV

Available Since

June 14, 2022

Availability

Academic Institutions and Nonprofits only

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pLib6.4B-Cquin_596

Plasmid

#176669

Purpose

Expression of sgRNA under C. quinquefasciatus U6 (CPIJ039596) promoter & delivery of D.mel-Actin5C::EBFP2-2A-puro|sgRNA cassette through PhiC31 RCME to AttP acceptor cell line/organism

Depositor

Norbert Perrimon

Article

Viswanatha et al Nat Commun. 2021 Nov 24;12(1):6825. doi:

Type

Empty backbone

Use

Crispr

Expression

Insect

Available Since

Nov. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pQUAST>stop>shibire (ts1)

Plasmid

#24363

Depositor

Liqun Luo

Article

Potter et al Cell. 2010 Apr 30. 141(3):536-48.

Insert

Shibire (ts1) (shi Fly)

Expression

Insect

Available Since

April 30, 2010

Availability

Academic Institutions and Nonprofits only

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DYNLT1

Plasmid

#132528

Purpose

Human TCTEX-1, codon optimised for Sf9 expression.

Depositor

Andrew Carter

Article

Toropova et al Nat Struct Mol Biol. 2019 Aug 26. pii: 1

Insert

DYNLT1 (DYNLT1 Human)

Expression

Insect

Mutation

Codon optimised for Sf9 expression

Available Since

Oct. 28, 2019

Availability

Academic Institutions and Nonprofits only

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pUASP dsRed Rab11 DN

Plasmid

#44831

Depositor

Matthew Scott

Article

Zhang et al Genetics. 2007 Jun;176(2):1307-22. Epub

Insert

Rab11 (Rab11 Fly)

Use

Drosophila

Tags

dsRed

Expression

Insect

Mutation

S25N (dominant negative)

Available Since

April 18, 2013

Availability

Academic Institutions and Nonprofits only
pFasBac+GFP-CAMSAP2 CC+CKK

Plasmid

#59039

Purpose

for baculovirus expression of CAMSAP2 CC+CKK in Sf9 cells

Depositor

Ron Vale

Article

Hendershott et al Proc Natl Acad Sci U S A. 2014 Apr 22;11

Insert

CAMSAP2 CC+CKK domains (CAMSAP2 Human)

Tags

His and eGFP

Expression

Insect

Available Since

Aug. 27, 2014

Availability

Academic Institutions and Nonprofits only

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pUASP dsRed Rab7 CA

Plasmid

#44825

Depositor

Matthew Scott

Article

Zhang et al Genetics. 2007 Jun;176(2):1307-22. Epub

Insert

Rab7 (Rab7 Fly)

Use

Drosophila

Tags

dsRed

Expression

Insect

Mutation

Q67L (constitutive active)

Available Since

April 18, 2013

Availability

Academic Institutions and Nonprofits only
pAC-luc-TimRA

Plasmid

#33133

Depositor

Michael Rosbash

Article

Kadener et al Genes Dev. 2009 Sep 15;23(18):2179-91. E

Insert

Tim-RA 3' UTR (tim Fly)

Use

Luciferase

Tags

Firefly Luciferase

Expression

Insect

Promoter

Actin 5C

Available Since

Jan. 23, 2012

Availability

Academic Institutions and Nonprofits only
pAc-HT-Dmp53

Plasmid

#85698

Purpose

expression of Drosophila p53 in Drosophila cells

Depositor

Licio Collavin, Giannino Del Sal

Article

Mauri et al J Biol Chem. 2008 Jul 25. 283(30):20848-

Insert

Dmp53 (p53 Fly)

Tags

His

Expression

Insect

Promoter

Ac5

Available Since

March 21, 2017

Availability

Academic Institutions and Nonprofits only
pENTR-GFP-Patronin CC+CKK

Plasmid

#59048

Purpose

for baculovirus expression of Patronin CC+CKK (using Baculodirect kit from Invitrogen)

Depositor

Ron Vale

Article

Hendershott et al Proc Natl Acad Sci U S A. 2014 Apr 22;11

Insert

Patronin (Patronin Fly)

Tags

eGFP

Expression

Bacterial and Insect

Available Since

Sept. 4, 2014

Availability

Academic Institutions and Nonprofits only