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Myc-Rab35 WT

Plasmid

#47433

Depositor

Peter McPherson

Article

Allaire et al J Cell Sci. 2013 Feb 1;126(Pt 3):722-31.

Insert

Rab35 (RAB35 Human)

Tags

Myc

Expression

Mammalian

Promoter

CMV

Available Since

July 29, 2014

Availability

Academic Institutions and Nonprofits only

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pAAV-rBrokenheart

Plasmid

#203394

Purpose

AAV transfer plasmid containing a constitutive (non Cre-dependent) BrokenHeart construct: a piggyBac donor transposon interrupting tdTomato. Transposase activity rescues coding sequence.

Depositor

Joseph Dougherty, Rob Mitra

Article

Yen et al Curr Protoc. 2023 Sep;3(9):e883. doi: 10

Insert

Brokenheart

Use

AAV

Expression

Mammalian

Promoter

EF1a

Available Since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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aMHC-Puro Rex-Blast

Plasmid

#21231

Depositor

Mark Mercola

Article

Kita-Matsuo et al PLoS ONE. 2009 . 4(4):e5046.

Insert

alpha myosin heavy chain promoter

Use

Lentiviral

Available Since

July 22, 2009

Availability

Academic Institutions and Nonprofits only

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pUPD P35S (GB0552)

Plasmid

#160554

Purpose

35s promoter for N-terminal fusions

Depositor

Diego Orzaez

Article

Vazquez-Vilar et al Nucleic Acids Res. 2017 Feb 28;45(4):219

Insert

35s

Use

Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available Since

Nov. 25, 2020

Availability

Academic Institutions and Nonprofits only

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pDONR221_eGFP (open)

Plasmid

#160094

Purpose

Expresses eGFP in mammalian cells

Depositor

Rizwan Haq

Article

Gstalder et al Cancer Discov. 2020 May 5. pii: 2159-829

Insert

eGFP

Use

Gateway entry vector

Expression

Mammalian

Available Since

Oct. 13, 2020

Availability

Academic Institutions and Nonprofits only

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sh-SRF

Plasmid

#100797

Purpose

expression of shRNA targeting SRF

Depositor

Joseph Gordon

Article

Mughal et al Cell Death Dis. 2015 Oct 29;6:e1944. doi

Insert

rat SRF

Use

RNAi

Promoter

H1

Available Since

Sept. 11, 2017

Availability

Academic Institutions and Nonprofits only

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10XUAS IVS ChrimsonR mCherry_tr

Plasmid

#111547

Purpose

Optogenetics in Drosophila: UAS-induced expression of ChrimsonR mCherry

Depositor

Vivek Jayaraman

Article

Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

Insert

ChrimsonR_mCherry_trafficked

Expression

Insect

Mutation

wt

Promoter

hsp70

Available Since

June 26, 2018

Availability

Academic Institutions and Nonprofits only

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RV-Cag-Dio-EYFP-P2A-cMaf

Plasmid

#98174

Purpose

Retroviral vector encoding Cre-dependent expression of EYFP-P2A-Maf

Depositor

Oscar Marin

Article

Mi et al Science. 2018 Feb 22. pii: science.aar68

Insert

cMaf (codon optimzed) (Maf Mouse)

Use

Retroviral

Mutation

Codon optimized

Promoter

CAG

Available Since

July 20, 2017

Availability

Academic Institutions and Nonprofits only

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pDRIVE-CAG-hFX-HA

Plasmid

#14994

Depositor

Michael Ristow

Article

Schulz et al Aging (Albany NY). 2010 Nov;2(11):843-53

Insert

human frataxin cDNA (FXN Human)

Tags

AU1 and HA

Expression

Mammalian

Available Since

Aug. 17, 2007

Availability

Academic Institutions and Nonprofits only

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pCAG-Venus-MDGA1

Plasmid

#82543

Purpose

Overexpression vector for Venus-MDGA1 in neurons

Depositor

Alice Ting

Article

Loh et al Cell. 2016 Aug 25;166(5):1295-1307.e21.

Insert

MDGA1 (MDGA1 Human)

Tags

Venus fluorescent protein

Expression

Mammalian

Promoter

chicken beta actin

Available Since

Sept. 21, 2016

Availability

Academic Institutions and Nonprofits only

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Frt-V5-hspB7

Plasmid

#63108

Purpose

Expression of HSPB7 (small HSP) tagged with V5 in mammalian cells

Depositor

Harm Kampinga

Article

Vos et al Hum Mol Genet. 2010 Dec 1;19(23):4677-93

Insert

hspb7 (HSPB7 Human)

Tags

V5

Expression

Mammalian

Promoter

CMV

Available Since

March 12, 2015

Availability

Academic Institutions and Nonprofits only

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pAAVS1-PdTK-CAG-mCh-[uBglII]

Plasmid

#107280

Purpose

puro-deltaTK gene-trap selection cassette (AAVS1 donor vector backbone) with constitutive mCherry

Depositor

Knut Woltjen

Article

Kim et al Nat Commun. 2018 Mar 5;9(1):939. doi: 10

Insert

AAVS1 puro-deltaTK; CAG-mCherry (PPP1R12C )

Available Since

March 16, 2018

Availability

Academic Institutions and Nonprofits only

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dCas9-DNMT3L

Plasmid

#126588

Purpose

DNMT3L fused to C-terminus of dCas9; pCDNA3 vector backbone, mammalian expression

Depositor

David Segal

Article

O'Geen et al Epigenetics Chromatin. 2019 May 3;12(1):

Insert

DNMT3L (DNMT3L Human)

Use

CRISPR

Tags

3XFLag-NLS-dCas9-DNMT3L-NLS

Expression

Mammalian

Available Since

Dec. 12, 2021

Availability

Academic Institutions and Nonprofits only

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A*33:01:01

Plasmid

#203288

Purpose

Overexpress HLA allele

Depositor

Derin Keskin, Catherine Wu

Article

Sarkizova et al Nat Biotechnol. 2020 Feb;38(2):199-209.

Insert

A*33:01:01 (HLA-A Human)

Expression

Mammalian

Available Since

July 10, 2023

Availability

Academic Institutions and Nonprofits only

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pTwist-WIV1-CoV

Plasmid

#164438

Purpose

Encodes WIV1-CoV Spike Protein for pseudovirus production

Depositor

Alejandro Balazs

Article

Garcia-Beltran et al Cell. 2021 Jan 21;184(2):476-488.e11. do

Insert

WIV1-CoV Spike

Expression

Mammalian

Available Since

May 20, 2021

Availability

Academic Institutions and Nonprofits only

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pBp-FGFR3c-R248C

Plasmid

#45712

Depositor

Matthew Meyerson

Article

Liao et al Cancer Res. 2013 Jun 20.

Insert

FGFR3 IIIc (FGFR3 Human)

Use

Retroviral

Expression

Mammalian

Mutation

R248C

Available Since

June 12, 2013

Availability

Academic Institutions and Nonprofits only
pLVTHM-shRnd3

Plasmid

#86437

Purpose

2nd generation doxycycline-inducible lentiviral vector expressing Rnd3-targeting shRNA and GFP

Depositor

Violaine Moreau

Article

Grise et al Hepatology. 2012 Jun;55(6):1766-75. doi:

Insert

shRNA targeting RND3 (RND3 Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Promoter

H1

Available Since

Feb. 1, 2017

Availability

Academic Institutions and Nonprofits only

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pTRE-TIGHT-Cx43-eYFP

Plasmid

#31807

Depositor

Robin Shaw

Article

Smyth et al J Clin Invest. 2010 Jan 4. 120(1):266-79

Insert

Cx43 (GJA1 Human)

Tags

eYFP

Expression

Mammalian

Promoter

P-TIGHT

Available Since

Sept. 14, 2011

Availability

Academic Institutions and Nonprofits only
p2xFLAGhYAP2-S127A

Plasmid

#17794

Depositor

Marius Sudol

Article

Komuro et al J Biol Chem. 2003 Aug 29. 278(35):33334-

Insert

Yes-kinase associated protein (YAP1 Human)

Tags

2xFlag

Expression

Mammalian

Mutation

Serine 127 to Alanine

Available Since

May 9, 2008

Availability

Academic Institutions and Nonprofits only
pHAGE2-TetOminiCMV-cMyc

Plasmid

#136614

Purpose

Dox-inducible lentiviral vector expressing mouse cMyc

Depositor

Hans Schöler

Article

Velychko et al Cell Stem Cell. 2019 Oct 30. pii: S1934-

Insert

cMyc (Myc Mouse)

Use

Lentiviral

Expression

Bacterial

Promoter

tetO-miniCMV (dox-inducible)

Available Since

March 25, 2020

Availability

Academic Institutions and Nonprofits only

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