We narrowed to 13,181 results for: OVA

1. pET51b(+)_EGFP-LAMA-R59 (for ALFA-tag)

   Plasmid

   #136627

   Purpose

   Plasmid encoding the bacterial expression of LAMA-R59 for ALFA-tag as an EGFP-fusion

   Depositor

   Kai Johnsson

   Article

   Farrants et al Nat Methods. 2020 Feb 17. pii: 10.1038/s

   Insert

   EGFP-LAMA-R59
   

   Use

   Tags

   Hisx10 and Strep

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Feb. 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pJAT11

   Plasmid

   #204290

   Purpose

   For inserting a phiC31 attP site. HDR integrant is marked with removable ie1-DsRed cassette, which is expressed in abdomen and mouthparts.

   Depositor

   David Stern

   Article

   Stern et al bioRxiv 2023.06.17.545412

   Insert

   ie1-DsRed
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   ie1

   Available since

   Sept. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET22b-6h-GST-TEVp

   Plasmid

   #172887

   Purpose

   Codon optimized (E. coli) TEVp fused with GST and his-tagged for purification

   Depositor

   Urartu Seker

   Article

   Ahan et al ACS Synth Biol. 2019 Apr 19;8(4):686-696

   Insert

   TEV protease
   

   Use

   Tags

   GST solubility tag and HHHHHH

   Expression

   Bacterial

   Mutation

   Promoter

   T7 promoter

   Available since

   July 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET28a_T7-ARG2

   Plasmid

   #106474

   Purpose

   Expresses acoustic reporter genes or gas vesicles (ARG2) in Escherichia coli

   Depositor

   Mikhail Shapiro

   Article

   Bourdeau et al Nature. 2018 Jan 3;553(7686):86-90. doi:

   Insert

   ARG2
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   March 23, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pThermeleon

   Plasmid

   #86120

   Purpose

   Bandpass temperature-gated expression of mWasabi and mCherry reporters

   Depositor

   Mikhail Shapiro

   Article

   Piraner et al Nat Chem Biol. 2017 Jan;13(1):75-80. doi

   Inserts

   TlpA
   mWasabi
   TcI38
   mCherry
   cI
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   A67T, M1V, L65S, K68R, F115L, D126G, D188G and D3…

   Promoter

   pR/pL, pR/pL, pLacI, and pTlpA

   Available since

   March 8, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAR357_SFP_pCDF-1b

   Plasmid

   #122851

   Purpose

   Co-expresses the phosphopantheteine transferase Sfp of Bacillus subtilis to transfer a PPant arm to acyl carrier proteins in Escherichia coli. Untagged; in pCDF-1b vector.

   Depositor

   Martin Grininger

   Article

   Rittner et al Protein Sci. 2019 Feb;28(2):414-428. doi

   Insert

   Sfp
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7 promoter

   Available since

   March 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pScPPX2

   Plasmid

   #112877

   Purpose

   Expresses His-tagged yeast exopolyphosphatase for purification from E. coli

   Depositor

   Michael Gray, Ursula Jakob

   Article

   Gray et al Mol Cell. 2014 Mar 6;53(5):689-99. doi:

   Insert

   PPX1 (PPX1 Budding Yeast)
   

   Use

   Tags

   6X His tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Aug. 23, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pACYC-T7-Nla-repeat-BbsI

   Plasmid

   #180215

   Purpose

   For cloning Neisseria lactamica Type I-C crRNA for protein purification in E.Coli

   Depositor

   Yan Zhang

   Article

   Tan et al Mol Cell. 2022 Feb 17;82(4):852-867.e5.

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   March 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET28a-TRBP

   Plasmid

   #50351

   Purpose

   bacterial expression of TRBP

   Depositor

   Kumiko Ui-Tei

   Article

   Takahashi et al PLoS One. 2013 May 2;8(5):e63434. doi: 1

   Insert

   TRBP (TARBP2 Human)
   

   Use

   Tags

   His and Myc

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Jan. 15, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET21b-MBPx-MBPx-SpyTag003

    Plasmid

    #216291

    Purpose

    Expresses tandem MBPx linked to-SpyTag003 in bacterial cells. MBPx is a variant of MBP (A312V I317V Δ172-173 Δ175-176) with enhanced affinity for maltose.

    Depositor

    Mark Howarth

    Article

    Driscoll et al Nat Commun. 2024 Mar 16;15(1):2403. doi:

    Insert

    MBPx-MBPx-SpyTag003
    

    Use

    Affinity Reagent/ Antibody

    Tags

    His6 and SpyTag003

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available since

    Oct. 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET15b T7pCONS TIR-2 sfGFP

    Plasmid

    #154468

    Purpose

    pET15b with a design flaw in the T7 promoter fixed, and a synthetically evolved TIR. Contains the coding sequence for sfGFP

    Depositor

    Daniel Daley

    Article

    Shilling et al Commun Biol. 2020 May 7;3(1):214. doi: 1

    Insert

    super folder GFP
    

    Use

    Tags

    His-thrombin

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    July 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. 6xHis(TEV)-Drp1-StrepII

    Plasmid

    #174422

    Purpose

    Expresses human Drp1 Isoform 3 in bacteria

    Depositor

    Thomas Pucadyil

    Article

    Kamerkar et al Nat Commun. 2018 Dec 7;9(1):5239. doi: 1

    Insert

    Drp1
    

    Use

    Tags

    6xHis(TEV) and StrepII

    Expression

    Bacterial

    Mutation

    None

    Promoter

    T7

    Available since

    Sept. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. 6xHis(TEV)-Drp1(K38A)-StrepII

    Plasmid

    #174423

    Purpose

    Expresses human Drp1 Isoform 3 with K38A mutation in bacteria

    Depositor

    Thomas Pucadyil

    Article

    Kamerkar et al Nat Commun. 2018 Dec 7;9(1):5239. doi: 1

    Insert

    Drp1
    

    Use

    Tags

    6xHis(TEV) and StrepII

    Expression

    Bacterial

    Mutation

    K38A

    Promoter

    T7

    Available since

    Sept. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET28b-His tagged nuclear Pif1

    Plasmid

    #65047

    Purpose

    expression of the nuclear form of Pif1p (Pif1p-delta40), Nterminal His tag

    Depositor

    Virgina Zakian, Jean-Baptiste Boulé

    Article

    Boule et al Nature. 2005 Nov 3;438(7064):57-61. Epub

    Insert

    Yeast Pif1, nuclear form, N-terminal 6x His-tag (PIF1 Budding Yeast)
    

    Use

    Tags

    histag

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available since

    Feb. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET11d-hUba2

    Plasmid

    #53136

    Purpose

    E.coli expression of full length untagged human Uba2 (SAE2)

    Depositor

    Frauke Melchior

    Article

    Pichler et al Cell. 2002 Jan 11;108(1):109-20.

    Insert

    UBA2 (UBA2 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Sept. 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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16. SSB-wt

    Plasmid

    #197260

    Purpose

    wild type E. coli SSB in pET21 expresses in BL21 under T7 control with lactose-amp resistance

    Depositor

    Timothy Lohman

    Article

    Antony et al J Mol Biol. 2013 Nov 29;425(23):4802-19.

    Insert

    wt E. coli SSB
    

    Use

    Tags

    none

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET28C-SARS-CoV-2-Nucleocapsid

    Plasmid

    #204402

    Purpose

    E. coli expression of full-length SARS-CoV-2 nucleocapsid

    Depositor

    Samie Jaffrey

    Article

    Dumelie et al Nat Chem Biol. 2023 Nov 16. doi: 10.1038

    Insert

    SARS-CoV-2 nucleocapsid (N SARS-CoV-2)
    

    Use

    Tags

    6x-His

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Sept. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pET28a_T5-ARG1

    Plasmid

    #106476

    Purpose

    Expresses acoustic reporter genes or gas vesicles (ARG1) in Escherichia coli Nissle 1917

    Depositor

    Mikhail Shapiro

    Article

    Bourdeau et al Nature. 2018 Jan 3;553(7686):86-90. doi:

    Insert

    ARG1
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T5

    Available since

    March 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pET28a-SpySrtA

    Plasmid

    #51139

    Purpose

    expression plasmid for N-terminal his tagged S.pyogenes SrtA

    Depositor

    Hidde Ploegh

    Article

    Guimaraes et al Nat Protoc. 2013 Sep;8(9):1787-99. doi:

    Insert

    S.pyogenes SrtA
    

    Use

    Tags

    6x His

    Expression

    Bacterial

    Mutation

    Deleted amino acids 1-80

    Promoter

    T7

    Available since

    Feb. 14, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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20. pMH610

    Plasmid

    #131862

    Purpose

    Produces holo-PhyB* in E. coli

    Depositor

    Wilfried Weber

    Article

    Horner et al ACS Synth Biol. 2019 Sep 26. doi: 10.102

    Inserts

    PhyB(1-651)-RGD-His6-Cys
    HO1
    PcyA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter-1 and T7 promoter-2

    Available since

    Oct. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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21. pDC158

    Plasmid

    #115793

    Purpose

    Expresses Rat SNAP25

    Depositor

    Axel Brunger

    Article

    Lai et al Neuron. 2017 Aug 2;95(3):591-607.e10. do

    Insert

    SNAP25 (Snap25 Rat)
    

    Use

    Tags

    hexa histidine

    Expression

    Bacterial

    Mutation

    c84s, c85s, c90s, c92s

    Promoter

    T7

    Available since

    Oct. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pDC191

    Plasmid

    #115792

    Purpose

    Expresses Rat synaptobrevin 2

    Depositor

    Axel Brunger

    Article

    Lai et al Neuron. 2017 Aug 2;95(3):591-607.e10. do

    Insert

    Synaptobrevin2 (Vamp2 Rat)
    

    Use

    Tags

    hexa histidine

    Expression

    Bacterial

    Mutation

    S28C and C103S (please see comments below)

    Promoter

    T7

    Available since

    Oct. 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pET32-sLatherin

    Plasmid

    #61685

    Purpose

    bacterial expression of the horse sweat/saliva protein Latherin as a His-tagged thioredoxin fusion

    Depositor

    Brian Smith

    Article

    Vance et al J R Soc Interface. 2013 Jun 19;10(85):20

    Insert

    Latherin (LATH horse)
    

    Use

    Tags

    HIS and Trx-Tag thioredoxin protein

    Expression

    Bacterial

    Mutation

    Codon optimized for E.coli

    Promoter

    T7

    Available since

    March 2, 2015

    Availability

    Academic Institutions and Nonprofits only

24. pET-Cre-6xHis

    Plasmid

    #62939

    Purpose

    Expression of Cre-6xHis in bacterial of cells

    Depositor

    David Liu

    Article

    Zuris et al Nat Biotechnol. 2015 Jan;33(1):73-80. do

    Insert

    Cre recombinase
    

    Use

    Tags

    6xHis tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    June 15, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pET23a-HsRPS27a

    Plasmid

    #69561

    Purpose

    Bacterial expression; in vitro transcription/translation (T7)

    Depositor

    Jorge Eduardo Azevedo

    Article

    Grou et al Sci Rep. 2015 Aug 3;5:12836. doi: 10.103

    Insert

    RPS27A (RPS27A Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Dec. 11, 2015

    Availability

    Academic Institutions and Nonprofits only

26. DT044A

    Plasmid

    #159405

    Purpose

    Inducible Perfringolysin O (PFO) expression and purification, controlled under hybrid IPTG-regulated T7/LacO promoter (pRT30)

    Depositor

    Sheref Mansy

    Article

    Toparlak et al Sci Adv. 2020 Sep 18;6(38). pii: 6/38/ea

    Insert

    pr.T7-LacO-6xHis-PFO (Pfo, Clostridium perfringens)
    

    Use

    Synthetic Biology

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    T7/LacO promoter

    Available since

    Feb. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pKL002

    Plasmid

    #98921

    Purpose

    pET DUET backbone with PROPS voltage indicator and GCaMP6f calcium sensor in the two MCS.

    Depositor

    Joel Kralj

    Article

    Bruni et al Proc Natl Acad Sci U S A. 2017 Aug 29;11

    Inserts

    PROPS
    GCaMP6f
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pET11a-CA(A14C, E45C)

    Plasmid

    #79045

    Purpose

    Bacterial vector for cloning and propagation in DH5alpha

    Depositor

    Wesley Sundquist

    Article

    Li et al Elife. 2016 Jun 2;5. pii: e16269. doi: 1

    Insert

    CA (A14C, E45C)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    A14C and E45C mutations in HIV-1 CA protein

    Promoter

    T7

    Available since

    March 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pET26b-Granzyme A (S-A)

    Plasmid

    #8825

    Depositor

    Judy Lieberman

    Article

    Beresford et al Proc Natl Acad Sci U S A 1997 Aug 19;94(

    Insert

    Human Granzyme A inactive (GZMA Human)
    

    Use

    Tags

    (His)6

    Expression

    Bacterial

    Mutation

    changed Serine 184 to Alanine

    Promoter

    Available since

    June 22, 2006

    Availability

    Academic Institutions and Nonprofits only

30. pET28a T7pCONS TIR-1 sfGFP

    Plasmid

    #154463

    Purpose

    pET28a with a design flaw in the T7 promoter fixed, and a synthetically evolved TIR. Contains the coding sequence for sfGFP

    Depositor

    Daniel Daley

    Article

    Shilling et al Commun Biol. 2020 May 7;3(1):214. doi: 1

    Insert

    super folder GFP
    

    Use

    Tags

    His-thrombin

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    July 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge