We narrowed to 12,359 results for: NSI
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Plasmid#165132Purposemammalian expression and localizationDepositorAvailable SinceFeb. 18, 2021AvailabilityAcademic Institutions and Nonprofits only
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pDisplay-EGFP-CAAX
Plasmid#208683PurposeExpresses the membrane-localized enhanced green fluorescent protein EGFP-CAAX in mammalian cellsDepositorInsertmembrane-localized enhanced green fluorescent protein EGFP-CAAX
ExpressionMammalianPromoterCMVAvailable SinceOct. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-GRAB_SST1.0
Plasmid#208665PurposeExpresses the genetically-encoded fluorescent somatostatin (SST) sensor GRAB_SST1.0 in neuronsDepositorInsertGPCR activation based somatostatin (SST) sensor GRAB_SST1.0
UseAAVPromoterhSynAvailable SinceOct. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
SpySwitch
Plasmid#184225PurposeExpresses SpySwitch in the bacterial cytoplasm. SpySwitch binds SpyTag-, SpyTag002- or SpyTag003-fusions non-covalently for affinity purification, allowing gentle pH or temperature release.DepositorInsertSpySwitch
TagsHis6ExpressionBacterialMutationE77A mutation prevents isopeptide bond formation,…PromoterT7Available SinceJune 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-GRAB_NPY1.0
Plasmid#208676PurposeExpresses the genetically-encoded fluorescent neuropeptide Y (NPY) sensor GRAB_NPY1.0 in neuronsDepositorInsertGPCR activation based neuropeptide Y (NPY) sensor GRAB_NPY1.0
UseAAVPromoterhSynAvailable SinceDec. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
p2T-U6-sgCTG
Plasmid#232723PurposeSpCas9 guide RNA targeting CAG repeats for C-to-T base editingDepositorInsertSpCas9 gRNA targeting CAG repeats
ExpressionMammalianAvailable SinceMarch 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
p2T-U6-sgGAA
Plasmid#232724PurposeCas9 guide RNA targeting GAA repeats for A-to-G base editingDepositorInsertSpCas9 gRNA targeting GAA repeats
ExpressionMammalianAvailable SinceMarch 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
pGL4.23-5xE-box
Plasmid#206841PurposeThe plasmid contains a 312 bp DNA fragment that contained 5 canonical E-boxes (GCCACGTGCA) spaced by 50 nucleotides and cloned into pGL4.23[luc2/minP] (XhoI/HindIII).DepositorInsert5x E-box ((GCCACGTGCA)
UseLuciferaseAvailable SinceJan. 27, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAS_4xU6-PylT M15 (UUA) FLAG-Mma PylRS
Plasmid#154774Purposeplasmid with 4xMma PylT (M15 mutant, UUA anticodon) cassette and Mma PylRS for ochre suppression in transient or stable, piggybac-mediated, integrationDepositorInsertMma PylRS (pylS Methanosarcina mazei)
TagsFLAGExpressionMammalianMutationin PylT: UUA anticodon and G10A, G14U, G16U, C20U…PromoterEF1Available SinceOct. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGEX6p1-GST-Rnf31
Plasmid#63134PurposeExpression of human RNF31/HOIP codon optimized for E. coli and insect cellsDepositorInsertRnf31 (RNF31 Human, Synthetic)
TagsGSTExpressionBacterialMutationSynthetic based on human protein sequencePromotertacAvailable SinceMarch 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
Mito-PyronicSF/pCMV-myc-mito
Plasmid#124813PurposeHighly resposive GFP-based pyruvate nanosensor to explore mitochondrial metabolism.DepositorInsertPyronicSF
TagsMitochondrial Sequence SignalExpressionMammalianPromoterCMVAvailable SinceMay 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
FUW-TetO-Tead4
Plasmid#128832Purposedox-dependent expression of mouse Tead4DepositorInsertTead4 (Tead4 Mouse)
UseLentiviralAvailable SinceSept. 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLKO_FOXA1_#1
Plasmid#70095PurposeExpression of shRNA to human FOXA1, puromycin selectionDepositorAvailable SinceDec. 1, 2015AvailabilityAcademic Institutions and Nonprofits only -
AAVS1-PDL1
Plasmid#159280PurposeHuman AAVS1 targeting vector for knockin of a CAGGS promoter-driven human PDL1/CD274 (cloned between AgeI and PacI). Targeted cells will be puromycin resistant.DepositorInsertHuman PDL1/CD274 (CD274 Human)
UseAAV; S1 knockin donor vectorExpressionMammalianPromoterCAGGSAvailable SinceSept. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLentiPGK Blast DEST JNKKTRmRuby2
Plasmid#59154PurposeLentiviral vector to express JNK KTR mRuby2 under PGK promoter (With Blasticidin Resistance)DepositorInsertJNK Kinase Translocation Reporter (MAPK8 Human)
UseLentiviralTagsmRuby2ExpressionMammalianPromoterPGKAvailable SinceSept. 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAS_4xG1 PylT FLAG-G1 PylRS Y125A
Plasmid#154769Purposeplasmid with 4xG1 PylT cassette and G1 PylRS Y125A for amber suppression in transient or stable, piggybac-mediated, integrationDepositorInsertG1 PylRS (MJ_RS07805 methanogenic archaeon mixed culture ISO4-G1)
TagsFLAGExpressionMammalianMutationY125APromoterEF1Available SinceOct. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1 shETV5-B
Plasmid#74978PurposeshETV5 shRNADepositorAvailable SinceMay 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLKO.5 shETV5-A
Plasmid#74977PurposeshETV5 shRNADepositorAvailable SinceMay 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
MCP-MSN
Plasmid#210704PurposeThis plasmid express EF1a promoter driven MCP-MSN transactivation moduleDepositorInsertMCP fused to transactivation domain from Human transcription factor MRTF-A, STAT-1 and NRF2
UseLentiviralTagsEGFPExpressionMammalianMutationN55K in MCPPromoterEF1aAvailable SinceNov. 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLX_TRC311 ETV4-L
Plasmid#74982PurposeConstitutive ETV4 ORF, blasticidin selectionDepositorAvailable SinceJuly 7, 2016AvailabilityAcademic Institutions and Nonprofits only