We narrowed to 26,138 results for: Vit

1. mCardinal-MannII-N-10

   Plasmid

   #56167

   Purpose

   Localization: Mannosidase/Golgi, Excitation: 604, Emission: 659

   Depositor

   Michael Davidson

   Article

   Chu et al Nat Methods. 2014 Mar 16. doi: 10.1038/n

   Insert

   MannII
   

   Use

   Tags

   mCardinal

   Expression

   Mammalian

   Mutation

   aa 1-112 of MannII

   Promoter

   CMV

   Available since

   March 4, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

2. pcDNA5 FRT/TO Plk4 KD ∆24-mCherry

   Plasmid

   #80272

   Purpose

   mammalian expression plasmid for kinase dead Plk4 with multi-phospho degron deleted

   Depositor

   Andrew Holland

   Article

   Moyer et al J Cell Biol. 2015 Jun 22;209(6):863-78.

   Insert

   Plk4 (PLK4 Human)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   deletion of multi-phospho degron (amino acids 282…

   Promoter

   CMV

   Available since

   Aug. 2, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3.1(+)-FluoSTEP-EKAR

   Plasmid

   #181858

   Purpose

   Genetically encoded FRET-based sensor for monitoring ERK activity near a protein of interest. Must pair with GFP11-tagged POI to reconstitute donor fluorescence.

   Depositor

   Jin Zhang

   Article

   Tenner et al Sci Adv. 2021 May 21;7(21). pii: 7/21/ea

   Insert

   FluoSTEP-EKAR
   

   Use

   Tags

   mRuby2 and sfGFP(1-10)

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pBEG R2-eGFP-L3 #BGV201

   Plasmid

   #48979

   Purpose

   Supplies the selection marker (eGFP) and IRES with flanked by AttR2 and AttL3 sites for Gateway cloning recombination reaction. (This corresponds to a module 3 plasmid).

   Depositor

   David Dankort

   Article

   Geiling et al PLoS One. 2013 Oct 11;8(10):e76279. doi:

   Insert

   eGFP
   

   Use

   Gateway cloning vector

   Tags

   IRES (strong)

   Expression

   Mutation

   Promoter

   NA

   Available since

   June 16, 2014

   Availability

   Academic Institutions and Nonprofits only

5. pJ207 Delta-cpc-KanR

   Plasmid

   #51468

   Purpose

   Deletes the cpc operon in Synechocystis and confers kanamycin resistance

   Depositor

   Anastasios Melis

   Article

   Kirst et al Biochim Biophys Acta. 2014 Oct;1837(10):

   Insert

   Replacing the cpc operon with kanamycin resistance
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Synechocystis endogenous cpc-operon promoter

   Available since

   Nov. 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pX458-Ef1a-Cas9-GFP (gRNA: Ascl1-3)

   Plasmid

   #159658

   Purpose

   CRISPR/Cas9 expressing plasmid containing the gRNA targeting mouse Ascl1.

   Depositor

   Joseph Brzezinski

   Article

   Kaufman et al Development. 2021 Jun 15;148(12). pii: 2

   Insert

   hSpCas9
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Ef1a

   Available since

   March 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pX458-Ef1a-Cas9-GFP (gRNA: Ascl1-2)

   Plasmid

   #159657

   Purpose

   CRISPR/Cas9 expressing plasmid containing the gRNA targeting mouse Ascl1.

   Depositor

   Joseph Brzezinski

   Article

   Kaufman et al Development. 2021 Jun 15;148(12). pii: 2

   Insert

   hSpCas9
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Ef1a

   Available since

   March 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. AB.pCCL.sin.cPPT.U6.miR-223-Decoy.hPGK.GFP.WPRE

   Plasmid

   #46601

   Depositor

   Brian Brown

   Article

   Mullokandov et al Nat Methods. 2012 Jul 1;9(8):840-6. doi:

   Insert

   miR-223 Decoy (MIR223, Mir223 Human, Mouse)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   hU6

   Available since

   Aug. 29, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pAAV-DR1-NRSE-RFP

   Plasmid

   #22927

   Depositor

   Edward Callaway

   Article

   Nathanson et al Front Neural Circuits. 2009 Nov 9;3:19.

   Insert

   peroxisome proliferator activated receptor gamma binding site promoter with neuron-restrictive silencer element with minimal CMV
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 25, 2010

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pRSETa-Amrose v1

    Plasmid

    #79537

    Purpose

    pRSETa (Invitrogen) plasmid expressing Amrose variant 1

    Depositor

    Randolph Caldwell

    Article

    Schoetz et al PLoS One. 2014 Sep 5;9(9):e107069. doi:

    Insert

    Amrose
    

    Use

    Tags

    6xHis, T7 epitope, and Xpress tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    July 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pBEG R2-iNeo-L3 #BGV207

    Plasmid

    #48986

    Purpose

    Supplies the selection marker (neomycin resistance) and IRES with flanked by AttR2 and AttL3 sites for Gateway cloning recombination reaction. (This corresponds to a module 3 plasmid).

    Depositor

    David Dankort

    Article

    Geiling et al PLoS One. 2013 Oct 11;8(10):e76279. doi:

    Insert

    neomycin resistance
    

    Use

    Gateway cloning vector

    Tags

    IRES (weak)

    Expression

    Mutation

    Promoter

    NA

    Available since

    June 16, 2014

    Availability

    Academic Institutions and Nonprofits only

12. vsv-CenpB 1-166-Sgo1 N61I-GFP

    Plasmid

    #108499

    Purpose

    expression of vsv-CenpB 1-166-Sgo1 N61I-EGFP (no PP2A binding)

    Depositor

    Susanne Lens

    Article

    Meppelink et al Cell Rep. 2015 Apr 28;11(4):508-15. doi:

    Insert

    CenpB-Shugoshin 1 (SGO1 Human)
    

    Use

    Tags

    EGFP and VSV

    Expression

    Mammalian

    Mutation

    CenpB 1-166 fusion before Sgo1, no PP2A binding N…

    Promoter

    CMV

    Available since

    May 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pFBD-Akt1(K14A)

    Plasmid

    #86562

    Purpose

    Expresses human full-length Akt1(K14A) mutant

    Depositor

    Thomas Leonard, Ivan Yudushkin

    Article

    Ebner et al Mol Cell. 2017 Feb 2;65(3):416-431.e6. d

    Insert

    Akt1 (AKT1 Human)
    

    Use

    Tags

    10xHis

    Expression

    Insect

    Mutation

    K14A mutation in the PH domain

    Promoter

    Available since

    March 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3myc-PR-LBD

    Plasmid

    #89113

    Purpose

    mammalian expression of human progesterone receptor ligand binding domain amino acid residues 632-933 with N-terminal myc tag

    Depositor

    Elizabeth Wilson

    Article

    Liu et al J Biol Chem. 2011 Aug 26;286(34):29951-6

    Insert

    human progesterone receptor ligand binding domain (PGR Human)
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    contains Ligand Binding Domain (632-933)

    Promoter

    CMV

    Available since

    April 6, 2017

    Availability

    Academic Institutions and Nonprofits only

15. pVLAD6-mmPtch1-B-SBP

    Plasmid

    #120886

    Purpose

    BacMam vector for Ptch1-B expression

    Depositor

    Philip Beachy

    Article

    Zhang et al Cell. 2018 Nov 15;175(5):1352-1364.e14.

    Insert

    ptch1 (Ptch1 Mouse)
    

    Use

    Tags

    SBP

    Expression

    Mammalian

    Mutation

    deleted amino acids 620-643, truncated at 1291

    Promoter

    CMV

    Available since

    Jan. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. AB.pCCL.sin.cPPT.U6.miR-146a-Decoy.hPGK.GFP.WPRE

    Plasmid

    #46590

    Depositor

    Brian Brown

    Article

    Mullokandov et al Nat Methods. 2012 Jul 1;9(8):840-6. doi:

    Insert

    miR-146a Decoy (MIR146A, Mir146 Human, Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    hU6

    Available since

    Aug. 29, 2013

    Availability

    Academic Institutions and Nonprofits only

17. LentiCRISPRv2puro-Gnaq

    Plasmid

    #170302

    Purpose

    A knock-out vector for the mouse Gnaq

    Depositor

    Michiyuki Matsuda

    Article

    Konishi et al Cancer Res. 2021 May 25. pii: 0008-5472.

    Insert

    A gRNA targeting the mouse Gnaq gene.
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. Sumo3 PolQM1 deltains3

    Plasmid

    #78459

    Purpose

    Inducible expression in E. coli

    Depositor

    Susan Wallace, Sylvie Doublie

    Article

    Hogg et al J Mol Biol. 2011 Jan 21;405(3):642-52. d

    Insert

    Polymerase theta (POLQ Human)
    

    Use

    Tags

    His and SUMO3

    Expression

    Bacterial

    Mutation

    delta 1-1791; delta 2497-2529

    Promoter

    T7

    Available since

    July 19, 2016

    Availability

    Academic Institutions and Nonprofits only

19. LZRSpBMN-­Z‐GFP‐Eps8

    Plasmid

    #74935

    Purpose

    Expresses mouse Eps8 fused to GFP

    Depositor

    Giorgio Scita

    Article

    Frittoli et al Immunity. 2011 Sep 23;35(3):388-99. doi:

    Insert

    Eps8 (Eps8 Mouse)
    

    Use

    Retroviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 11, 2016

    Availability

    Academic Institutions and Nonprofits only

20. pBEG R2-eCFP-L3 #BGV202

    Plasmid

    #48977

    Purpose

    Supplies the selection marker (eCFP) and IRES with flanked by AttR2 and AttL3 sites for Gateway cloning recombination reaction. (This corresponds to a module 3 plasmid).

    Depositor

    David Dankort

    Article

    Geiling et al PLoS One. 2013 Oct 11;8(10):e76279. doi:

    Insert

    eCFP
    

    Use

    Gateway cloning vector

    Tags

    IRES (strong)

    Expression

    Mutation

    Promoter

    NA

    Available since

    June 16, 2014

    Availability

    Academic Institutions and Nonprofits only