We narrowed to 15,838 results for: grna

1. PXPR007 sgGFP-1

   Plasmid

   #74960

   Purpose

   Cas9 + sgGFP-1 with blasticidin selection

   Depositor

   William Hahn

   Article

   Wang et al Cell Rep. 2017 Feb 7;18(6):1543-1557. do

   Insert

   GFP
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   May 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pUC CBA-SpCas9D10Anickase.EF1a-BFP.sgLMNApair4

   Plasmid

   #98975

   Purpose

   Cas9 Homologous Recombination Reporter. SpCas9D10A nickase and a pair of sgRNAs targeting hLMNA. Generates breaks with 94bp 3' overhangs. TagBFP.

   Depositor

   Jan Karlseder

   Article

   Arnoult et al Nature. 2017 Sep 20;549(7673):548-552. d

   Insert

   LMNA sgRNAs pair 4 and Cas9(D10A)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Nov. 29, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. B270 + SPRTN sgSTOP

   Plasmid

   #100718

   Purpose

   B270 plasmid expressing SPRTN sgSTOP (cloned in BbsI site) in combination with ATP1A1 sgSTOP

   Depositor

   Alberto Ciccia

   Article

   Billon et al Mol Cell. 2017 Sep 4. pii: S1097-2765(17

   Insert

   sgSTOP targeting SPRTN (cloned using BbsI)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6 promoters

   Available Since

   Sept. 19, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSMP-EZH1_1

   Plasmid

   #36359

   Depositor

   George Daley

   Article

   Onder et al Nature. 2012 Mar 4;483(7391):598-602. do

   Insert

   EZH1 (EZH1 Human)
   

   Use

   RNAi and Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   May 2, 2012

   Availability

   Academic Institutions and Nonprofits only

5. pPV-H1-ccdB-mEF1α-RiH

   Plasmid

   #100598

   Purpose

   piggyBac vector for sgRNA cloning

   Depositor

   Akitsu Hotta

   Article

   Ishida et al Sci Rep. 2018 Jan 10;8(1):310. doi: 10.1

   Type

   Empty backbone

   Use

   Piggybac vector

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Human H1 PolIII promoter, human FTH1 promoter, mo…

   Available Since

   April 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pSMP-G9A_1

   Plasmid

   #36395

   Depositor

   George Daley

   Article

   Onder et al Nature. 2012 Mar 4;483(7391):598-602. do

   Insert

   G9A (EHMT2 Human)
   

   Use

   RNAi and Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   May 2, 2012

   Availability

   Academic Institutions and Nonprofits only

7. pHS1398

   Plasmid

   #205971

   Purpose

   MWEE01000013 Cas5-HNH crRNA expression in pCOLADuet-1

   Depositor

   Feng Zhang

   Article

   Altae-Tran et al Science. 2023 Nov 24;382(6673):eadi1910.

   Insert

   Cas5-HNH crRNA
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Nov. 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pHS1182

   Plasmid

   #205964

   Purpose

   NZ_CP025815 DinG HNH crRNA expression in pCOLADuet-1

   Depositor

   Feng Zhang

   Article

   Altae-Tran et al Science. 2023 Nov 24;382(6673):eadi1910.

   Insert

   DinG HNH crRNA
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Nov. 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pSMP-Dot1L_1

   Plasmid

   #36339

   Depositor

   George Daley

   Article

   Onder et al Nature. 2012 Mar 4;483(7391):598-602. do

   Insert

   Dot1L (DOT1L Human)
   

   Use

   RNAi and Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   May 2, 2012

   Availability

   Academic Institutions and Nonprofits only

10. MSCV P2Gm p53

    Plasmid

    #19751

    Depositor

    Richard Hynes

    Article

    Stern et al Proc Natl Acad Sci U S A. 2008 Sep 16. 1

    Insert

    oligo targeting p53 (Trp53 Mouse)
    

    Use

    RNAi and Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Dec. 18, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pSico-shRaptor

    Plasmid

    #81086

    Purpose

    conditional expression of an shRNA targeting murine Raptor

    Depositor

    Angelique Bordey

    Article

    Hartman et al Cell Rep. 2013 Oct 31;5(2):433-44. doi:

    Insert

    Raptor shRNA (Rptor Mouse)
    

    Use

    Cre/Lox, Lentiviral, and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    Sept. 27, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. tdTomato/pTREX-b

    Plasmid

    #68709

    Purpose

    Expresses tdTomato in pTREX-b vector which confers resistance to blasticidin. This vector is used for cloning a specific sgRNA by BamHI, to transfect Cas9-expressing Trypanosoma cruzi epimastigotes.

    Depositor

    Roberto Docampo

    Article

    Lander et al MBio. 2015 Jul 21;6(4). pii: e01012-15.

    Insert

    tdTomato
    

    Use

    Expression of tdtomato red fluorescence protein i…

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Oct. 8, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

13. pSimpleII-U6-tracr-U6-crRNA(tdTomato)-NLS-NmCas9-HA-NLS(s)

    Plasmid

    #47869

    Purpose

    All-in-one plasmid targeting tdTomato

    Depositor

    Erik Sontheimer, James Thomson

    Article

    Hou et al Proc Natl Acad Sci U S A. 2013 Aug 12.

    Inserts

    NmCas9
    U6pr-tracrRNA
    tdTomato crRNA
    

    Use

    CRISPR

    Tags

    HA and NLS

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Sept. 30, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. shHIF2α(#2) (HIF2α-#3-pSUPER-retro-GFP/Neo)

    Plasmid

    #22132

    Depositor

    William Kaelin

    Article

    Li et al Mol Cell Biol. 2007 Aug . 27(15):5381-92

    Insert

    synthethic oligonucleotide
    

    Use

    RNAi and Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    synthethic oligonucleotide encoding shRNA targeti…

    Promoter

    Available Since

    Feb. 1, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. p-mCherry2-sgMUC4

    Plasmid

    #198399

    Purpose

    Expresses a guide RNA against a repetitive locus found in the MUC4 gene of human chromosome 3, with mCherry2 reporter

    Depositor

    Sarah McClelland

    Article

    Tovini et al EMBO J. 2023 May 15;42(10):e111587. doi:

    Insert

    MUC4 sgRNA (MUC4 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    April 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. RT3GEN

    Plasmid

    #111165

    Purpose

    Tet-regulated miR-E (miR-30 variant)-based RNAi with tGFP reporter

    Depositor

    Johannes Zuber

    Article

    Fellmann et al Cell Rep. 2013 Dec 26;5(6):1704-13. doi:

    Insert

    miR-E (miR-30 variant)
    

    Use

    Retroviral

    Tags

    Expression

    Mutation

    WT

    Promoter

    T3G

    Available Since

    May 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pSicoR-TMF1i2

    Plasmid

    #59625

    Purpose

    Expression of shRNA against human TMF1

    Depositor

    Miguel Ramalho-Santos

    Article

    Qin et al Cell. 2014 Jul 17;158(2):449-61. doi: 10

    Insert

    TMF1 (TMF1 Human)
    

    Use

    RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    Sept. 29, 2014

    Availability

    Academic Institutions and Nonprofits only

18. pSicoR-TMF1i1

    Plasmid

    #59624

    Purpose

    Expression of shRNA against human TMF1

    Depositor

    Miguel Ramalho-Santos

    Article

    Qin et al Cell. 2014 Jul 17;158(2):449-61. doi: 10

    Insert

    TMF1 (TMF1 Human)
    

    Use

    RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    Sept. 29, 2014

    Availability

    Academic Institutions and Nonprofits only

19. pSMP-G9A_4

    Plasmid

    #36334

    Depositor

    George Daley

    Article

    Onder et al Nature. 2012 Mar 4;483(7391):598-602. do

    Insert

    G9A (EHMT2 Human)
    

    Use

    RNAi and Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    May 2, 2012

    Availability

    Academic Institutions and Nonprofits only

20. pLV-shKcnk13

    Plasmid

    #120721

    Purpose

    Expresses GFP and an shRNA targeting Kcnk13 (in pLL3.7)

    Depositor

    Amy Lasek

    Article

    You et al Neuropharmacology. 2018 Oct 14;144:29-36

    Insert

    Kcnk13 (Kcnk13 Mouse)
    

    Use

    Lentiviral and RNAi

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    mouse U6

    Available Since

    March 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pJG85

    Plasmid

    #89281

    Purpose

    Entry vector containing the TaU6 promoter and an Esp3I Golden Gate cloning site for cloning of the sgRNA, all between attL5 and attL2 sites

    Depositor

    Daniel Voytas

    Article

    Gil-Humanes et al Plant J. 2016 Dec 10. doi: 10.1111/tpj.1

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available Since

    May 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pLKO-puro human lipin 1-1 shRNA

    Plasmid

    #32019

    Depositor

    David Sabatini

    Article

    Peterson et al Cell. 2011 Aug 5;146(3):408-20.

    Insert

    lipin1 shRNA
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    Sept. 27, 2011

    Availability

    Academic Institutions and Nonprofits only

23. shMEK1/2 v2 neo

    Plasmid

    #72570

    Purpose

    Hairpin targeting MEK1 and MEK2 (murine).

    Depositor

    Kevin Janes

    Article

    Jensen et al Cell Syst. 2016 Feb 24;2(2):112-121.

    Insert

    MEK1/2 (Map2k1, Map2k2 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    July 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pSMP-SUZ12_1

    Plasmid

    #36390

    Depositor

    George Daley

    Article

    Onder et al Nature. 2012 Mar 4;483(7391):598-602. do

    Insert

    SUZ12 (SUZ12 Human)
    

    Use

    RNAi and Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    May 2, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pLKO.1P shFXN_1

    Plasmid

    #102970

    Purpose

    Suppression of FXN

    Depositor

    Richard Possemato

    Article

    Alvarez et al Nature. 2017 Nov 22. doi: 10.1038/nature

    Insert

    shFXN_1 (FXN Human)
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Dec. 18, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. LEP-shMLL-AF9.1039

    Plasmid

    #105566

    Purpose

    retrovirally express MLL-AF9 shRNA with puro resistance and GFP marker

    Depositor

    Christopher Vakoc

    Article

    Xu et al Cancer Cell. 2018 Jan 8;33(1):13-28.e8.

    Insert

    shRNA targeting MLL part of MLL-AF9 fusion
    

    Use

    RNAi and Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    MSCV-LTR

    Available Since

    Feb. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. LEP-shMLL-AF9.643

    Plasmid

    #105565

    Purpose

    retrovirally express MLL-AF9 shRNA with puro resistance and GFP marker

    Depositor

    Christopher Vakoc

    Article

    Xu et al Cancer Cell. 2018 Jan 8;33(1):13-28.e8.

    Insert

    shRNA targeting MLL part of MLL-AF9 fusion
    

    Use

    RNAi and Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    MSCV-LTR

    Available Since

    Feb. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. shPBRM1 #122-Tet-pLKO-puro

    Plasmid

    #107401

    Purpose

    Lentiviral expression of shRNA targeting PBRM

    Depositor

    William Kaelin

    Article

    Gao et al Proc Natl Acad Sci U S A. 2017 Jan 31;11

    Insert

    shRNA targeting PBRM1 (PBRM1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    June 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. MSCV FLIPi P2G_Thy1.1 PTEN

    Plasmid

    #19749

    Depositor

    Richard Hynes

    Article

    Stern et al Proc Natl Acad Sci U S A. 2008 Sep 16. 1

    Insert

    oligo targeting PTEN (Pten Mouse)
    

    Use

    Cre/Lox, RNAi, and Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Dec. 18, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. Tol2-LyzC-GFP-U6ac-ctrl-guides

    Plasmid

    #168240

    Purpose

    "neutrophil specific GFP with ubiquitous ctrl sgRNAs"

    Depositor

    Qing Deng

    Article

    Wang et al J Cell Sci. 2021 Apr 15;134(8). pii: 237

    Insert

    control sgRNAs
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Feb. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge