164,577 results

1. pDG02583

   Plasmid

   #104129

   Purpose

   Optimized bacterial expression plasmid of bdNEDP1 protease (Frey and Goerlich, 2014)

   Depositor

   Dirk Görlich

   Article

   Pleiner et al J Cell Biol. 2018 Mar 5;217(3):1143-1154

   Insert

   NEDD8-specific protease 1 (NEDP1)
   

   Use

   Tags

   14x Histidine tag, Maltose binding protein, and S…

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Dec. 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. 3xHA-TurboID_pAS31

   Plasmid

   #118220

   Purpose

   expresses 3xHA-tagged TurboID in C. elegans intestine

   Depositor

   Jessica Feldman

   Article

   Branon et al Nat Biotechnol. 2018 Aug 20. pii: nbt.42

   Insert

   TurboID (BirA mutant)
   

   Use

   Tags

   3x HA tag

   Expression

   Worm

   Mutation

   Q65P, I87V, R118S, E140K, Q141R, S150G, L151P, V1…

   Promoter

   ges-1p

   Available Since

   Jan. 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCAG_GPHN.FingR-mKate2-IL2RGTC

   Plasmid

   #46297

   Depositor

   Don Arnold

   Article

   Gross et al Neuron. 2013 Jun 19;78(6):971-85. doi: 1

   Insert

   GPHN.FingR-mKate2-IL2RGTC (GPHN Synthetic, Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available Since

   Nov. 14, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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4. pExp-Lipo

   Plasmid

   #129237

   Purpose

   Protein expression in E.coli

   Depositor

   Marko Hyvönen

   Type

   Empty backbone

   Use

   Tags

   His and Strep

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Aug. 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCFJ2440 - [1-2] ENTR - Fluor - ce-GFP(PATCs(900bp), no_atg, no stop)

   Plasmid

   #159849

   Purpose

   Three-fragment Gateway compatible flourophore for expression in C. elegans

   Depositor

   Christian Froekjaer-Jensen

   Article

   Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

   Insert

   [1-2] ENTR - Fluor - ce-GFP(PATCs(900bp), no_atg, no stop)
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Not applicable

   Promoter

   Available Since

   Dec. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pHH0103_CAPNS1_EF-hand_8+EF-hand_5

   Plasmid

   #109995

   Purpose

   Protein expression and purification of CAPNS1_EF-hand_8+EF-hand_5

   Depositor

   Sachdev Sidhu

   Article

   Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

   Insert

   CAPNS1_EF-hand_8+EF-hand_5 (CAPNS1 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Oct. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMRE-Tn7-160

   Plasmid

   #118572

   Purpose

   miniTn7 plasmid to deliver constitutively expressed fluorescent protein genes in bacteria

   Depositor

   Mitja Remus-Emsermann

   Article

   Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

   Insert

   TagBFP2
   

   Use

   Minitn7 delivery vector

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Oct. 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. RFP-LC3B

   Plasmid

   #200943

   Purpose

   RFP fused with LC3B for reporting LC3B lipidation

   Depositor

   Nir Hacohen

   Article

   Liu et al Science. 2023 Aug 4;381(6657):508-514. d

   Insert

   LC3B (MAP1LC3B Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Sept. 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pENTR-THT

   Plasmid

   #55790

   Purpose

   GATEWAY cloning compatible conditional shRNA expression vector; TetO flanked H1 RNA gene promoter; Kanamycin resistance

   Depositor

   Stephan Geley

   Article

   Sigl et al PLoS One. 2014 May 19;9(5):e97764. doi:

   Type

   Empty backbone

   Use

   RNAi; Gateway

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   modified H1 RNA gene

   Available Since

   Nov. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. TFORF0346

    Plasmid

    #143397

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    MYT1L (MYT1L Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available Since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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