-
Plasmid#20018DepositorInsertNfkb1 (Nfkb1 Mouse)
UseTagsFlagExpressionMammalianMutationTruncated at the p105 ligation site. It codes for…PromoterAvailable sinceJan. 28, 2009AvailabilityAcademic Institutions and Nonprofits only -
PrimeRoot.v2C-FLP
Plasmid#201740PurposeFor DNA insertion test in protoplastDepositorInsertePPE,FLP
UseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceJuly 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHR-SFFV_3C-Twin-Strep
Plasmid#113900PurposeMammalian protein expressionDepositorTypeEmpty backboneUseLentiviralTags3C-Twin-StrepExpressionMutationPromoterSFFVAvailable sinceAug. 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHTNW
Plasmid#136403PurposeExpression of N-terminal HaloTag-fused proteins in mammalian cells. Genes can be cloned using Gateway cloning system. Cloned vectors can be used for NanoBRET assay.DepositorTypeEmpty backboneUseTagsHaloTagExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
-
Integrin alpha5-AHCys
Plasmid#27313DepositorInsertintegrin alpha 5 (ITGA5 Human)
UseTagsExpressionMammalianMutationintegrin alpha5 (1-954) + AHCysPromoterAvailable sinceMarch 8, 2011AvailabilityAcademic Institutions and Nonprofits only -
pFB2D
Plasmid#195286PurposeBicistronic DTR-EGFP fusion and Puromycin resistance for positive/negative selectionDepositorInsertDTR EGFP 2A Puro
UseTagsExpressionMammalianMutationPromoterEFSAvailable sinceJan. 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Venus-Venus-FLARE-Cameleon
Plasmid#123351PurposeYellow-fluorescent homoFRET/anisotropy-based genetically encoded calcium indicator.DepositorInsertVenus-Venus-FLARE-Cameleon
UseTags6xHIS, T7 tag (gene 10 leader), Venus, and Xpress…ExpressionMammalianMutationPromoterCMVAvailable sinceJuly 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSLIK-PUM2-rABE-Flag
Plasmid#191386PurposeDoxycycline inducible expression of PUM2-rABEDepositorInsertPUM2-rABE (PUM2 Human, Synthetic)
UseLentiviralTagsFlagExpressionMammalianMutationPromotertet-inducible promoterAvailable sinceNov. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1 puro_shDDX58_230212
Plasmid#167293PurposeLentiviral plKO.1 construct coding for a short-hairpin RNA targeting the human gene DDX58 (coding for RLR sensor RIG-I). Contains a puro resistance cassette.DepositorInsertshRNA hsDDX58 (RIGI Human)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceApril 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
XRE-pNL1.3[secNLuc]
Plasmid#182295PurposeSecreted Luciferase reporter for AhR activityDepositorInsertXenobiotic Response Element (CYP1A1 Human)
UseTagssecNlucExpressionMammalianMutationPromoterXREAvailable sinceOct. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
Strep-KIFC1*-mCh
Plasmid#120169PurposeExpresses a chimera of streptavidin, a KIFC1-based retrograde motor and mCherry (fluorescent tag)DepositorInsertkinesin family member C1 (KIFC1 Human)
UseTagsStreptavidin and mCherryExpressionMammalianMutationTruncated KIFC1: 125-673 aaPromoterCMVAvailable sinceJuly 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
pST-K
Plasmid#44560Purposefor ectopic constitutive expression of mycobacterial proteinsDepositorTypeEmpty backboneUseE.coli-mycobacteria shuttle vectorTagsFlag tag and His tagExpressionBacterialMutationPromoterUV15 promoterAvailable sinceOct. 30, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3 Flag MKK4
Plasmid#14615DepositorInsertMKK4 (MAP2K4 Human)
UseTagsFlagExpressionMammalianMutationPromoterAvailable sinceJuly 25, 2007AvailabilityAcademic Institutions and Nonprofits only -
SypHer
Plasmid#48250PurposepH sensorDepositorInsertSypHer
UseTagsExpressionMammalianMutationC199SPromoterCMVAvailable sinceOct. 28, 2013AvailabilityAcademic Institutions and Nonprofits only -
pBbE1k-DDC
Plasmid#71723Purposedopa decarboxylase from pigDepositorInsertdopa decarboxylase
UseTagsExpressionMutationPromoterpTrcAvailable sinceJan. 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAd5-B6/7 ∆E3-GFP
Plasmid#179202PurposeA block for the AdenoBuilder genome assembly system. The insert consolidates the regions present in pAd5-B6∆E3-GFP and pAd5-B7.DepositorInsertModified block 6/7, with deletion in E3 and insertion of GFP cassette
UseAdenoviralTagsExpressionMutationPromoterAvailable sinceJan. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
Brm WT-Flag pShuttle
Plasmid#122105PurposeExpression of full length Brm in mammalian cellsDepositorInsertBRM (SMARCA2 Human)
UseTagsFlagExpressionMammalianMutationPromoterAvailable sinceApril 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
Human Integrin beta 2
Plasmid#8640DepositorInsertIntegrin beta 2 (ITGB2 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 23, 2005AvailabilityAcademic Institutions and Nonprofits only -
pMOS003-lenti-CMV-GCaMPer
Plasmid#65227PurposeER calcium sensor for lentivirus productionDepositorInsertGCaMPer
UseLentiviralTagscircularly permuted GFPExpressionMammalianMutationGCaMP3 (D324G, D360G, D397G, D435G)PromoterCMVAvailable sinceMay 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSV40-mCherry-P2A-Hygro
Plasmid#106478PurposeExpression of mCherry and Hygromycin resistance gene from SV40 promoterDepositorInsertsmCherry
Hygromycin B resistance
UseTagsExpressionMammalian and PlantMutationPromoterSV40 promoterAvailable sinceApril 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMM136
Plasmid#127217PurposeBeYDV replicon with constitutive Luc expression, MP-delta, sgRNA targeting PDSDepositorInsertLuc, MP-delta, sgRNA
UseTagsExpressionPlantMutationcodon optimized CDSsPromoterAvailable sinceDec. 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDGM3B
Plasmid#110809Purposecontains the AAV3B cap genes, AAV2 rep genes, and also adenovirus helper genesDepositorInsertAAV3B cap genes, AAV2 rep genes, adenovirus helper genes
UseAAVTagsExpressionMutationPromoterAvailable sinceJune 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
AICSDP-166:CDH1-mEGFP
Plasmid#193921PurposeHomology arms and LE-mEGFP sequence for C-terminus tagging of human cadherin 1DepositorInsertcadherin 1 Homology Arms with LE-mEGFP (CDH1 Human)
UseAAV and CRISPR; Donor templateTagslinker-mEGFPExpressionMutationhomology arms contain point mutations to disrupt …PromoterAvailable sinceDec. 22, 2022AvailabilityIndustry, Academic Institutions, and Nonprofits -
pMAK464
Plasmid#140702PurposeNBαEGFPDepositorInsertNBαEGFP
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSimpleII-U6-tracr-U6-BsmBI-NLS-NmCas9-HA-NLS(s)
Plasmid#47868PurposeThis plasmid contains expression cassette for NmCas9 with N and C NLS and an HA tag, a cassette for expression of tracrRNA, a cassette for cloning crRNA under the control of U6 promoter.DepositorInsertsNmCas9
U6pr-tracrRNA
UseCRISPRTagsHA and NLSExpressionMammalianMutationPromoterEF1a and U6Available sinceSept. 30, 2013AvailabilityAcademic Institutions and Nonprofits only -
pLV-TetO-hNGN2-Neo
Plasmid#99378PurposeExpresses human NEUROGENIN2 (hNGN2) and neomycin resistance gene under control of TetON promoter. 3rd generation lentiviral backbone.DepositorInserthNGN2-P2A-Neo (NEUROG2 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterTetOAvailable sinceAug. 18, 2017AvailabilityAcademic Institutions and Nonprofits only -
pG4
Plasmid#162605PurposeEdit Ade2 gene in yeastDepositorInsertTef1-Cas9 with RPL25 Intron
UseTagsExpressionYeastMutationPromoterAvailable sinceJune 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
MIGR-RORgt
Plasmid#24069DepositorInsertRorgt (Rorc Mouse)
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceOct. 27, 2010AvailabilityAcademic Institutions and Nonprofits only -
pX330-AAVS1
Plasmid#227272PurposeExpresses SpCas9 and a sgRNA targeting the AAVS1 loci for knock-in.DepositorInsertsgRNA Targeting AAVS1 (AAVS1 Synthetic)
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceNov. 5, 2024AvailabilityAcademic Institutions and Nonprofits only