We narrowed to 21,477 results for: SPR

Showing: 7921 - 7940 of 21477 results

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pCDNA5 TEX264wt-cGFP

Plasmid

#220211

Purpose

Mammalian expression of wild type TEX264 fused at C-terminus to GFP

Depositor

Kristijan Ramadan

Article

Fielden et al Nat Commun. 2020 Mar 9;11(1):1274. doi:

Insert

TEX264 (TEX264 Human)

Tags

Strep-strep-HA

Expression

Mammalian

Promoter

CMV/TetO

Available Since

June 12, 2024

Availability

Academic Institutions and Nonprofits only

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SOX17-3'gRNA

Plasmid

#210465

Purpose

gRNA targeting SOX17 3' terminal for CRISPR-Cas9-mediated knock-in

Depositor

Kai Wang

Article

Zhao et al STAR Protoc. 2024 May 23;5(2):103084. do

Insert

SOX17 (SOX17 Human)

Use

CRISPR

Available Since

Jan. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pMS_375

Plasmid

#182132

Purpose

expresses retron RNA, ec.86 Reverse Transcriptase, CspRecT SSAP, and mutL* to create a specific rifampicin resistance mutation while inhibiting MMR

Depositor

George Church

Article

Schubert et al Proc Natl Acad Sci U S A. 2021 May 4;118

Inserts

retron RNA
ec.86 RT
CspRecT

Mutation

incorporated rifampicin-resistance-conferring don…

Available Since

Sept. 1, 2023

Availability

Academic Institutions and Nonprofits only

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pGL3-U6-sgRNA-PGK- mRFP-T2A-PuroR

Plasmid

#194925

Purpose

mRFP and T2A linker are inserted in between the hPGK promoter and the puromycin resistance gene (PuroR) on pGL3-U6-sgRNA-PGK-puromycin to allow simultaneous monitoring and enrichment of transfected ho

Depositor

Hao Xu

Article

Ayo et al J Genome Ed Regul. 2022;2:246146. doi: 1

Type

Empty backbone

Use

CRISPR

Promoter

hPGK

Available Since

May 30, 2023

Availability

Academic Institutions and Nonprofits only

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pEN-L1-PJ23119-PplpT-sfGFP-TrrfB-BsaI-Scaf-L2

Plasmid

#196250

Purpose

Plasmid for cloning of a CRISPR-Cas9 guide RNA gene under promoter PJ23119

Depositor

Bing Yang

Article

Li et al Commun Biol. 2023 Jan 17;6(1):56. doi: 1

Insert

attL1-GlpT-scGFP-attL2

Expression

Bacterial

Promoter

J23119 promoter

Available Since

May 12, 2023

Availability

Academic Institutions and Nonprofits only

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pLenti-NRCH-PE2-BSD

Plasmid

#191101

Purpose

Lentiviral expression plasmid of NRCH-PE prime editor with P2A-BSD marker

Depositor

Hyongbum Kim

Article

Yu et al Cell. 2023 Apr 21:S0092-8674(23)00331-8.

Insert

NRCH-PE2-BSD

Use

CRISPR and Lentiviral

Tags

SV40 NLS

Expression

Mammalian

Promoter

EF-1a

Available Since

May 2, 2023

Availability

Academic Institutions and Nonprofits only

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pHAGE-IRES-puro-NLS-NLS-dHgm4Cas13b-3xGFP-NLS

Plasmid

#191368

Purpose

Overexpression of NLS-NLS-dHgm4Cas13b-3xGFP-NLS in human cells

Depositor

Ling-Ling Chen

Article

Yang et al Cell Insight. 2022 Jun 15;1(4):100044. d

Insert

NLS-NLS-dHgm4Cas13b-3xGFP-NLS

Tags

3xGFP

Expression

Mammalian

Available Since

Oct. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pHAGE-IRES-puro-NLS-NLS-dPba3Cas13b-3xGFP-NLS

Plasmid

#191369

Purpose

Overexpression of NLS-NLS-dPba3Cas13b-3xGFP-NLS in human cells

Depositor

Ling-Ling Chen

Article

Yang et al Cell Insight. 2022 Jun 15;1(4):100044. d

Insert

NLS-NLS-dPba3Cas13b-3xGFP-NLS

Tags

3xGFP

Expression

Mammalian

Available Since

Oct. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pTR-177 pUC19-CLTA-N-mCherry

Plasmid

#186128

Purpose

knockin of mCherry to CLTA target

Depositor

Alexander Marson

Article

Shy et al Nat Biotechnol. 2022 Aug 25:10.1038/s415

Insert

CLTA (CLTA Human)

Use

CRISPR

Mutation

WT with mCherry sequence

Available Since

July 28, 2022

Availability

Academic Institutions and Nonprofits only

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Lenti-sgArid1a#1/Cre

Plasmid

#173571

Purpose

Expresses a Arid1a-targeting gRNA and Cre-recombinase

Depositor

Charles Swanton

Article

Cai et al Cancer Discov. 2021 Feb 19. pii: 2159-82

Insert

gRNA targeting Arid1a (Arid1a Mouse)

Use

CRISPR, Cre/Lox, and Lentiviral

Available Since

June 29, 2022

Availability

Academic Institutions and Nonprofits only

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eSpCas9(1.1)_No_FLAG_LMNA_G2

Plasmid

#178090

Purpose

Expresses the LMNA G2 sgRNA in combination with FLAGless eSpCas9(1.1). This vector can be used in combination with LMNA_mScarlet-I_Donor to tag LMNA with mScarlet-I. pX330-like plasmid.

Depositor

Yannick Doyon

Article

Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:

Insert

LMNA G2 sgRNA + FLAGless enhanced specificity Cas9 (1.1)

Use

CRISPR

Expression

Mammalian

Promoter

U6 promoter

Available Since

Dec. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pHS0667 KraIscB-1 non-coding locus in pCOLADuet-1

Plasmid

#176537

Purpose

Non-protein coding locus components from KraIscB-1

Depositor

Feng Zhang

Article

Altae-Tran et al Science. 2021 Oct;374(6563):57-65. doi:

Insert

non-coding locus components from KraIscB-1 locus

Expression

Bacterial

Available Since

Nov. 2, 2021

Availability

Academic Institutions and Nonprofits only

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pU6-SasgRNA scaffold-CMV-nSaCas9

Plasmid

#171695

Purpose

Expresses nSaCas9 in mammalian cells

Depositor

Jia Chen

Article

Wang et al Nat Cell Biol. 2021 May 10. pii: 10.1038

Insert

sgRNA scaffold-nSaCas9-NLS

Use

CRISPR

Expression

Mammalian

Mutation

D10A

Promoter

U6 and CMV

Available Since

Aug. 9, 2021

Availability

Academic Institutions and Nonprofits only

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pHSG1C3

Plasmid

#164423

Purpose

single guide RNA (sgRNA) and Prime editing guide RNA (pegRNA) cloning and mammalian cell expression. BbsI cloning for sgRNAs and BbsI/PstI for pegRNAs.

Depositor

Xiao Wang

Article

Standage-Beier et al ACS Synth Biol. 2021 Feb 19;10(2):422-42

Insert

sgRNA

Use

CRISPR and Synthetic Biology

Expression

Mammalian

Promoter

U6

Available Since

Aug. 4, 2021

Availability

Academic Institutions and Nonprofits only

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pDECKO_mCherry_non-targeting

Plasmid

#140109

Purpose

CRISPR-Cas9 library validation (negative control)

Depositor

Roderic Guigo

Article

Arnan et al BMC Genomics. 2022 May 26;23(1):402. doi

Insert

gRNA1+scaffold+H1promoter+gRNA2

Use

Lentiviral

Promoter

gRNA1 under U6 and gRNA2 under H1

Available Since

April 29, 2021

Availability

Academic Institutions and Nonprofits only

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pT7-SpCas9_sgRNA-site1 (RTW443)

Plasmid

#160136

Purpose

T7 promoter expression plasmid for in vitro transcription of SpCas9 sgRNA with spacer #1

Depositor

Keith Joung, Benjamin Kleinstiver

Article

Walton et al Nat Protoc. 2021 Feb 5. pii: 10.1038/s41

Insert

SpCas9 sgRNA with spacer #1 (spacer=GGGCACGGGCAGCTTGCCGG)

Use

In vitro transcription of sgrna from t7 promoter

Promoter

T7

Available Since

Feb. 8, 2021

Availability

Academic Institutions and Nonprofits only

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PHY98-LMNA 5'HA-TriTag (mTagBFP)-3'HA (HDR donor)

Plasmid

#164046

Purpose

CRISPR donor plasmid to insert TriTag (mTagBFP) into the N-terminus of human LMNA gene

Depositor

Baohui Chen

Article

Xu et al Nucleic Acids Res. 2020 Oct 26. pii: 594

Insert

mTagBFG-TriTag

Use

CRISPR

Expression

Mammalian

Available Since

Jan. 28, 2021

Availability

Academic Institutions and Nonprofits only

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pLenti-mU6-Luc2GO-PGK-Neo

Plasmid

#136905

Purpose

Lentivirus for base editing activatable Luciferase2 expression in mammalian cells. All in one vector with sgLuc2GO.

Depositor

Lukas Dow

Article

Katti et al Nucleic Acids Res. 2020 Feb 29. pii: 576

Insert

Luc2CyGo

Use

Lentiviral

Mutation

ATG>ACG

Promoter

SFFV

Available Since

Feb. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pORANGE GFP-CADPS KI

Plasmid

#131482

Purpose

Endogenous tagging of CAPS1: N-terminal (amino acid position: S39)

Depositor

Harold MacGillavry

Article

Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

Insert

gRNA and GFP donor (Cadps Rat)

Expression

Mammalian

Promoter

U6 and Cbh

Available Since

Sept. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pLC-CUL4A WT-P2A-Puro

Plasmid

#124304

Purpose

Lentiviral vector for expression of wild-type CUL4A-P2A-Puro casette from a CMV promoter

Depositor

Eva Gottwein

Article

Patil et al Blood Adv. 2019 Jul 23;3(14):2105-2117.

Insert

CUL4A (CUL4A Human)

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

July 22, 2019

Availability

Academic Institutions and Nonprofits only

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