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FUW-SM

Plasmid

#20330

Depositor

Rudolf Jaenisch

Article

Carey et al Proc Natl Acad Sci U S A. 2009 Jan 6. 10

Insert

Sox2-F2A-cMyc

Use

Lentiviral

Expression

Mammalian

Available Since

Feb. 23, 2009

Availability

Academic Institutions and Nonprofits only

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pICH51266

Plasmid

#50267

Depositor

Sylvestre Marillonnet, Nicola Patron

Article

Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

Insert

promoter (1.3 kb ), 35s (Cauliflower Mosaic Virus) + 5'UTR omega (Tobacco Mosaic Virus)

Use

Plant expression

Mutation

BsaI/BbsI sites removed by point-mutation

Available Since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

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pDN-LexAOx4-mNeonGreen

Plasmid

#192604

Purpose

Vector for inducible mNeonGreen expression with 4x LexA operator (LexAO)

Depositor

Yingxiao Wang

Article

Huang et al Sci Adv. 2020 Feb 19;6(8):eaay9209. doi:

Insert

mNeonGreen

Use

Unspecified

Promoter

Minimal promoter

Available Since

Nov. 7, 2022

Availability

Academic Institutions and Nonprofits only

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SpyCas9(WT)

Plasmid

#101044

Purpose

Express Streptococcus pyogenes Cas9

Depositor

Zhiwei Huang

Article

Dong et al Nature. 2017 Jun 15;546(7658):436-439. d

Insert

SpyCas9

Tags

GST

Expression

Bacterial

Available Since

Oct. 12, 2017

Availability

Academic Institutions and Nonprofits only

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pRC3EGFP

Plasmid

#193790

Purpose

Expresses EGFP under the control of PR promoter, p15A origin of replication, Chloramphenicol selection

Depositor

Sangram Bagh

Article

Sarkar et al J Biol Eng. 2019 Mar 1;13:20. doi: 10.11

Insert

EGFP

Expression

Bacterial

Promoter

PR promoter

Available Since

April 10, 2023

Availability

Academic Institutions and Nonprofits only

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pCAGGS_2paCRY2

Plasmid

#129651

Purpose

2P-activatable CRY2

Depositor

Michiyuki Matsuda

Article

Kinjo et al Nat Methods. 2019 Sep 9. pii: 10.1038/s4

Insert

2paCRY2

Tags

mTagBFP2

Expression

Mammalian

Promoter

CAG

Available Since

Sept. 12, 2019

Availability

Academic Institutions and Nonprofits only

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pCAG-Bmp5

Plasmid

#163589

Purpose

Expresses BMP-5 in mammalian cells

Depositor

Takeshi Imai

Article

Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

Insert

Bmp5 (Bmp5 Mouse)

Expression

Mammalian

Available Since

Aug. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pPOTv6-puro-3Myc:mScarletI:3Myc

Plasmid

#179804

Purpose

Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging

Depositor

Samuel Dean, Keith Gull

Article

Paterou et al Open Biol. 2025 Apr;15(4):240361. doi: 1

Insert

mScarlet-I

Use

Unspecified

Promoter

read through

Available Since

April 24, 2023

Availability

Academic Institutions and Nonprofits only

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pJMH143

Plasmid

#186646

Purpose

aadA.co-TaadA-PJ23117-eYFP.co between homology arms for pilA

Depositor

Adam Arkin

Article

Hilzinger et al Biotechnol Bioeng. 2025 Jan;122(1):44-52

Insert

aadA.co-TaadA-PJ23117-eYFP.co

Use

Synthetic Biology

Expression

Bacterial

Available Since

Jan. 12, 2023

Availability

Academic Institutions and Nonprofits only

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PAX6 TALEN R

Plasmid

#105525

Purpose

Knock in H2B-GFP into the human PAX6 locus

Depositor

Lorenz Studer

Article

Tchieu et al Cell Stem Cell. 2017 Sep 7;21(3):399-410

Insert

Pax6 TALEN R

Use

TALEN

Tags

3x FLAG and NLS

Expression

Mammalian

Promoter

CMV

Available Since

May 31, 2018

Availability

Academic Institutions and Nonprofits only

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PAX6 TALEN L

Plasmid

#109034

Purpose

Knock in H2B-GFP into the human PAX6 locus

Depositor

Lorenz Studer

Article

Tchieu et al Cell Stem Cell. 2017 Sep 7;21(3):399-410

Insert

Pax6 TALEN L

Use

TALEN

Tags

3x FLAG and NLS

Expression

Mammalian

Promoter

CMV

Available Since

May 4, 2018

Availability

Academic Institutions and Nonprofits only

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pFA6a-kanMX6-Purg1

Plasmid

#39349

Depositor

Jurg Bahler

Article

Watt et al PLoS One. 2008 Jan 16;3(1):e1428.

Insert

urg1 promoter (urg1, urg1 Fission Yeast)

Use

Yeast genomic targeting

Tags

kanMX6 module - reverse orientation

Available Since

Nov. 8, 2012

Availability

Academic Institutions and Nonprofits only
pMB62

Plasmid

#47944

Purpose

Expresses C. elegans optimized Cas9::mEGFP from the eef-1A.1 (eft-3) promoter

Depositor

Mike Boxem

Article

Waaijers et al Genetics. 2013 Aug 26.

Insert

Cas9

Use

CRISPR

Tags

3xFLAG, SV40 NLS, egl-13 NLS, and mEGFP

Expression

Worm

Promoter

eef-1A.1 (eft-3)

Available Since

Oct. 17, 2013

Availability

Academic Institutions and Nonprofits only

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pPB-R1R2_EF1aVP64dCas9p300_T2A_MS2p65HSF1-IRESbsdpA

Plasmid

#113344

Purpose

Expresses VP64-dCas9-p300 and MS2-p65-HSF1 fusion proteins

Depositor

Gavin Wright

Article

Chong et al Genome Biol. 2018 Nov 26;19(1):205. doi:

Insert

VP64-dCas9-p300core-T2A-MS2-p65-HSF1

Use

Piggybac

Available Since

Dec. 12, 2018

Availability

Academic Institutions and Nonprofits only

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pRE57I-GNAc

Plasmid

#170637

Purpose

DNA donor for transposition

Depositor

Sheng Yang

Article

Zhang et al CRISPR J. 2021 Jun;4(3):350-359. doi: 10

Insert

Mini-Tn6677, GNAc cassette, lacI

Expression

Bacterial

Available Since

Aug. 4, 2021

Availability

Academic Institutions and Nonprofits only

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pCAG-Bmp10

Plasmid

#163593

Purpose

Expresses BMP-10 in mammalian cells

Depositor

Takeshi Imai

Article

Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

Insert

Bmp10 (Bmp10 Mouse)

Expression

Mammalian

Available Since

Aug. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pJL-mGold

Plasmid

#157997

Purpose

Expression of mGold in yeast cells

Depositor

Francois St-Pierre

Article

Lee et al Sci Adv. 2020 Oct 23;6(43). pii: 6/43/ea

Insert

mGold

Expression

Yeast

Mutation

mGold is mVenus with L46F;T63S mutations

Promoter

pTDH(GAP promoter)

Available Since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pENTR11 mSTIM1 myc

Plasmid

#17733

Depositor

Anjana Rao

Article

Oh-Hora et al Nat Immunol. 2008 Apr . 9(4):432-43.

Insert

STIM1 (Stim1 Mouse)

Tags

Myc

Expression

Mammalian

Available Since

April 14, 2008

Availability

Academic Institutions and Nonprofits only
Wnt3A STOP

Plasmid

#35871

Depositor

Marian Waterman

Article

Najdi et al Differentiation. 2012 Sep;84(2):203-13.

Insert

Wnt3A (WNT3A Human)

Use

Gateway Cloning

Mutation

None

Available Since

May 7, 2013

Availability

Academic Institutions and Nonprofits only

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UbcH10 DN AU1

Plasmid

#8507

Depositor

Joan Ruderman

Article

Townsley et al Proc Natl Acad Sci U S A 1997 Mar 18;94(

Insert

UbcH10 (UBE2C Human)

Tags

AU1 (DTYRYI)

Expression

Mammalian

Mutation

C114S

Available Since

June 1, 2005

Availability

Academic Institutions and Nonprofits only