We narrowed to 9,896 results for: UTY

1. pAAVS1_EF1a-MitoTrap-mCherry-IRES-Puro

   Plasmid

   #197067

   Purpose

   Donor plasmid for human AAVS1 locus knock-in, constitutive expression of MitoTrap-mCherry

   Depositor

   Julia Mahamid

   Article

   Fung et al Nat Methods. 2023 Dec;20(12):1900-1908.

   Insert

   MitoTrap-mCherry
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Sept. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pHR-PGK-LexA-WW-CIB1-biLINuS

   Plasmid

   #171757

   Purpose

   Lentiviral vector for constitutive expression of fusion protein LexA-WW-CIB1-biLINuS

   Depositor

   Yingxiao Wang

   Article

   Huang et al Sci Adv. 2020 Feb 19;6(8):eaay9209. doi:

   Insert

   Synthetic gene containing LexA-WW-CIB1-biLINuS
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   PGK

   Available Since

   Sept. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3.1 human cofilin S3A

   Plasmid

   #50854

   Purpose

   expresses constituvely active cofilin in mammalian cells

   Depositor

   James Bamburg

   Article

   Garvalov et al J Neurosci. 2007 Nov 28;27(48):13117-29.

   Insert

   cofilin 1 S3A (CFL1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   constitutively active, S3A

   Promoter

   CMV

   Available Since

   Feb. 4, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

4. pJM600 EF1α PC 3xFLAG-FKBP-FGFR4-TEVp in TUPV2

   Plasmid

   #161570

   Purpose

   Constitutive expression of PC 3xFLAG-FKBP-FGFR4-TEVp under the EF1α promoter

   Depositor

   Joshua Leonard

   Article

   Muldoon et al Sci Adv. 2021 Feb 19;7(8). pii: 7/8/eabe

   Insert

   PC 3xFLAG-FKBP-FGFR4-TEVp
   

   Use

   Synthetic Biology

   Tags

   3x-FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   EF1α

   Available Since

   Jan. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pBabe puro Myr HA PIK3CB

   Plasmid

   #12527

   Depositor

   Jean Zhao

   Article

   Zhao et al Proc Natl Acad Sci U S A. 2005 Dec 20. 1

   Insert

   PIK3CB (PIK3CB Human)
   

   Use

   Retroviral

   Tags

   HA and Myr

   Expression

   Mammalian

   Mutation

   Myristoylation. Constitutively active allele.

   Promoter

   Available Since

   Nov. 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. nTRb412-gs10-NLS-VPR

   Plasmid

   #188267

   Purpose

   Plasmid ensures constitutive expression of the N-terminal fragment of split TRbeta (aa 200-412), fused to the VPR activation domain, a flexible GS linker and the SV40 NLS signal at the C-terminus

   Depositor

   Roman Jerala

   Article

   Rihtar et al Nat Chem Biol. 2022 Sep 26. doi: 10.1038

   Insert

   nTRb412 (TXNRD2 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Oct. 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pNHEJ-RPG

   Plasmid

   #85931

   Purpose

   Constitutively express DsRed and conditionally express Puro and EGFP. Used to test transfection efficiency and enrich genetically modified cells

   Depositor

   Zhiying Zhang

   Article

   Ren et al Cell Mol Life Sci. 2015 Jul;72(14):2763-

   Insert

   pPB-CMV-DsRed-polyA-CAG-NHEJ. Puro-T2A-EGFP
   

   Use

   Dual-reporter surrogate

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Feb. 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLX307 P2A GFP PRDM14

   Plasmid

   #193676

   Purpose

   Constitutive lentiviral expression of PRDM14

   Depositor

   William Hahn

   Article

   Kim et al Dev Cell. 2022 Jan 24;57(2):212-227.e8.

   Insert

   PRDM14 (PRDM14 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Jan. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. hCMV-IE1:GLuc:bgH

   Plasmid

   #135294

   Purpose

   Transcriptional unit for constitutive expression of GLuc in mammalian cells

   Depositor

   Koen Venken

   Article

   Sarrion-Perdigones et al Nat Commun. 2019 Dec 13;10(1):5710. doi:

   Insert

   CMV:GLuc:bGH
   

   Use

   Luciferase and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   May 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pSC101 NL4-3 envFS Δrev ΔRRE RTEm26CTE

    Plasmid

    #101351

    Purpose

    HIV-1 delta Rev was cloned into pSC101 and modified to include an RTEm26CTE element in order to utilize the NXF1 RNA export pathway. The RRE was also mutated.

    Depositor

    Jeremy Luban

    Article

    McCauley et al Nat Commun. 2018 Dec 13;9(1):5305. doi:

    Insert

    NL4-3
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    March 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pHelper-CBE-sgRNA

    Plasmid

    #221137

    Purpose

    Helper plasmid for sgRNA cloning for CRISPR-Cas9 cytosine base editing. sgRNA-scaffold with Cas9 handle expressed from constitutive bacterial promoter J23119(SpeI). 2 BbsI sites for sgRNA cloning.

    Depositor

    Craig Roy

    Article

    Steiner et al mSphere. 2024 Nov 19:e0052324. doi: 10.1

    Insert

    sgRNA-scaffold with Cas9 handle
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    J23119(SpeI)

    Available Since

    Nov. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMRE146

    Plasmid

    #118498

    Purpose

    broad-host range plasmid to constitutively express fluorescent protein genes in bacteria

    Depositor

    Mitja Remus-Emsermann

    Article

    Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

    Insert

    mCardinal
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Nov. 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET151-D-TOPO-PARP14-SΔK

    Plasmid

    #216658

    Purpose

    Expresses multidomain construct of PARP14 with internal SUMO domain (PARP14-SΔK) in bacterial cells.

    Depositor

    Herwig Schüler

    Article

    Chatzicharalampous et al Protein Expr Purif. 2024 Aug 16:106580.

    Insert

    PARP14-SΔK DNA sequence (PARP14 G784-K1808 with internal SUMO-TEV sequence) (PARP14 Human, Synthetic)
    

    Use

    Tags

    6xHis-V5-TEV

    Expression

    Bacterial

    Mutation

    Deletion starting from Q1390 to D1448 and substit…

    Promoter

    T7

    Available Since

    Aug. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. c3GIC9n

    Plasmid

    #62192

    Purpose

    col1a1 targeting vector for inducible [TRE3G]-GFP-IRES-Cas9n (D10A variant) expression. Contains NsiI cloning site for U6-sgRNA cassettes

    Depositor

    Lukas Dow

    Article

    Dow et al Nat Biotechnol. 2015 Apr;33(4):390-4. do

    Insert

    humanized S. Pyogenes Cas9 (D10A)
    

    Use

    CRISPR

    Tags

    HA

    Expression

    Mutation

    D10A substitution (Cas9n)

    Promoter

    TRE3G

    Available Since

    Aug. 10, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3.1V5-His Snk/Plk2 T239D

    Plasmid

    #22220

    Depositor

    Wafik El-Deiry

    Article

    Burns et al Mol Cell Biol. 2003 Aug . 23(16):5556-71

    Insert

    Snk/Plk2 (PLK2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    single amino acid substitution mutant, threonine …

    Promoter

    Available Since

    Aug. 27, 2012

    Availability

    Academic Institutions and Nonprofits only

16. pUB-sSypHer (RbcS-SypHer)

    Plasmid

    #84732

    Purpose

    Expresses SypHer in plant cells-biosensor for pH targeted to chloroplasts stoma

    Depositor

    Philip Mullineaux

    Article

    Exposito-Rodriguez et al Nat Commun. 2017 Jun 29;8(1):49. doi: 10

    Insert

    sSypHer
    

    Use

    Tags

    RbcS targeting sequence

    Expression

    Plant

    Mutation

    Promoter

    ubiqutin- 10 gene promoter (pUBQ10) of Arabidopsis

    Available Since

    July 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCR3.1 GSK3beta S9A HA

    Plasmid

    #15994

    Depositor

    Morris Birnbaum

    Article

    Summers et al J Biol Chem. 1999 Jun 18. 274(25):17934-

    Insert

    GSK3beta (GSK3B Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    S9A: constitutively active

    Promoter

    Available Since

    Oct. 26, 2007

    Availability

    Academic Institutions and Nonprofits only

18. pHES941

    Plasmid

    #87942

    Purpose

    Constitutively expresses progesterone-inducible synthetic transcription factor in yeast. It activates transcription of promoters containing Gal4 binding sites.

    Depositor

    Hana El-Samad

    Article

    Aranda-Diaz et al ACS Synth Biol. 2016 Dec 27. doi: 10.102

    Insert

    GAL4 DBD - hPR LBD - MSN2 AD (PGR Human, Synthetic, Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    May 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pSLQ8541 PB-PGK-RR12EE345L-C term (406) dLbCpf1-2XNLS-miniVPR-mCherry-pA

    Plasmid

    #140222

    Purpose

    Constitutive PGK driven expression of leucine zipper with C terminal dCpf1 (Cas12a) 406-split half fused to miniVPR

    Depositor

    Stanley Qi

    Article

    Kempton et al Mol Cell. 2020 Jan 30. pii: S1097-2765(2

    Insert

    zipper with dLbCpf1 (Cas12a) C terminal half (406) fused to miniVPR
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    D832A

    Promoter

    PGK

    Available Since

    May 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. RIPR-bio-His

    Plasmid

    #50824

    Purpose

    Expresses enzymatically monobiotinylated full length RIPR

    Depositor

    Gavin Wright

    Article

    Zenonos et al Malar J. 2014 Mar 12;13(1):93. doi: 10.1

    Insert

    RIPR
    

    Use

    Tags

    ratCD4d3+4,biotinylation sequence, 6xHis

    Expression

    Mammalian

    Mutation

    all potential N-linked glycosylation sites (N-X-S…

    Promoter

    CMV

    Available Since

    Feb. 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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