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Plasmid#199990PurposeYeast/E coli shuttle vector expressing GFP from tef1 promoterDepositorInsertGFP
UseTagsExpressionYeastMutationPromoterAvailable sinceSept. 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRSFDuet-1_mNeongreen-StefinA
Plasmid#182418PurposeBacterial expression of mNeongreen-stefinA fusionDepositorInsertmNeongreen-stefin (CSTA Human)
UseTagsmNeongreenExpressionBacterialMutationPromoterT7Available sinceFeb. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
p415 TEF roGFP2‐Tsa2ΔCR
Plasmid#83238PurposeThe genetically encoded fluorescent H2O2 sensor roGFP2-Tsa2ΔCR cloned in the yeast p415 vector under the control of a TEF promoter. For cytosolic expression.DepositorInsertroGFP2-Tsa2dCr
UseTagsroGFP2 fusion with Tsa2dCrExpressionYeastMutationPromoterTEFAvailable sinceSept. 30, 2016AvailabilityAcademic Institutions and Nonprofits only -
p414-TEF1p-Cas9-CYC1t
Plasmid#43802PurposeA human codon-optimized Cas9 for expression in S. cerevisiae (budding yeast) from the TEF1 promoterDepositorArticleInsertHuman Optimized S. pyogenes Cas9
UseCRISPRTagsExpressionYeastMutationPromoterTEF1 promoterAvailable sinceMarch 15, 2013AvailabilityAcademic Institutions and Nonprofits only -
p415 TEF roGFP2‐Tsa2ΔCPΔCR
Plasmid#83239PurposeThe catalytically inactive genetically encoded fluorescent H2O2 sensor roGFP2-Tsa2ΔCPΔCR cloned in the yeast p415 vector under the control of a TEF promoter. For cytosolic expression.DepositorInsertroGFP2-Tsa2dCpdCr
UseTagsroGFP2 fusion with Tsa2dCpdCrExpressionYeastMutationPromoterTEFAvailable sinceSept. 30, 2016AvailabilityAcademic Institutions and Nonprofits only -
pRS405-LEU2-TEF-KpolDcr1v2
Plasmid#85193PurposeIntegrating plasmid, K. polysporus Dcr1 under TEF promoterDepositorInsertDcr1
UseIntegrating, shuttle vectorTagsExpressionYeastMutationPromoterTEFAvailable sinceNov. 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pUC-UAS1B16-TEF(272)
Plasmid#44375DepositorInsertUAS1B16-TEF(272) promoter
UseSynthetic BiologyTagsExpressionBacterialMutationN/APromoterUAS1B16-TEF(272)Available sinceApril 18, 2013AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCfB2312 (TEF1p-Cas9-CYC1t_kanMX)
Plasmid#83946PurposeCas9-carrying vector with kanMX markerDepositorInsertCas9
UseTagsExpressionYeastMutationPromoterAvailable sinceOct. 27, 2016AvailabilityAcademic Institutions and Nonprofits only -
pUC-UAS1B8-TEF(272)
Plasmid#44376DepositorInsertUAS1B8-TEF(272) promoter
UseSynthetic BiologyTagsExpressionBacterialMutationN/APromoterUAS1B8-TEF(272)Available sinceApril 16, 2013AvailabilityIndustry, Academic Institutions, and Nonprofits -
pUC-UAS1B16-TEF(504)
Plasmid#44371DepositorInsertUAS1B16-TEF(504) promoter
UseSynthetic BiologyTagsExpressionBacterialMutationN/APromoterUAS1B16-TEF(504)Available sinceJuly 15, 2013AvailabilityIndustry, Academic Institutions, and Nonprofits -
pUC-UAS1B8-TEF(136)
Plasmid#44380DepositorInsertUAS1B8-TEF(136) promoter
UseSynthetic BiologyTagsExpressionBacterialMutationN/APromoterUAS1B8-TEF(136)Available sinceApril 17, 2013AvailabilityIndustry, Academic Institutions, and Nonprofits -
3xFLAG-dCas9/pTEF1p-CYC1t
Plasmid#62190PurposeExpresses 3xFLAG-dCas9 in budding yeast for enChIP analysis to purify specific genomic regions of interest.DepositorInsert3xFLAG-dCas9
UseCRISPRTags3xFLAG and NLSExpressionYeastMutationhuman codon-optimized, D10A, H840APromoterTEF1 promoterAvailable sinceFeb. 24, 2015AvailabilityAcademic Institutions and Nonprofits only -
pUC-UAS1B8-TEF(504)
Plasmid#44372DepositorInsertUAS1B8-TEF(504) promoter
UseSynthetic BiologyTagsExpressionBacterialMutationN/APromoterUAS1B8-TEF(504)Available sinceJuly 15, 2013AvailabilityIndustry, Academic Institutions, and Nonprofits -
p1977 (pUC19-PTDH3-PTEF1)
Plasmid#78230PurposeExample plasmid containing a bidirectional promoter of pTDH3 and pTEF1DepositorInsertBidirectional promoters pTDH3-pTEF1
UseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pPpT4- TEF2prom-TIR1F74G-FLAG
Plasmid#189727PurposeEnables expression of OsTIR1(F74G) from the TEF2 promoter, used to implement the AID2 system in Komagataella phaffii (original name pLB219)DepositorInsertOsTIR1
UseTagsFLAGExpressionYeastMutationPromoterTEF2Available sinceSept. 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pUC-UAS1B16-TEF(203)
Plasmid#44377DepositorInsertUAS1B16-TEF(203) promoter
UseSynthetic BiologyTagsExpressionBacterialMutationN/APromoterUAS1B16-TEF(203)Available sinceApril 16, 2013AvailabilityIndustry, Academic Institutions, and Nonprofits -
pPotef-LN22*-3xGNLS-cbx
Plasmid#86780PurposeExpresses lambda N protein fused to triple Gfp coupled to an NLS sequence under the control of the constitutively active Potef promotor.DepositorInsertLN*
UseUstilago maydis expressionTagsGfpExpressionBacterialMutationPromoterPotefAvailable sinceSept. 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCfB1975 (pUC19-PTEF1-PPGK1)
Plasmid#73296PurposeExample plasmid containing a bidirectional promoterDepositorInsertpTEF1-pPGK1
UseSynthetic BiologyTagsExpressionBacterial and YeastMutationPromoterAvailable sinceNov. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pRSFDuet-1_VNp-mNeongreen-StefinA
Plasmid#182419PurposeBacterial expression of Vesicle Nucleating peptide-mNeongreen-stefinA fusionDepositorInsertVNp-mNeongreen-stefin (CSTA Human)
UseTagsVesicle Nucleating peptide (VNp) & mNeongreenExpressionBacterialMutationPromoterT7Available sinceFeb. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
POT5-TEF2p-ycrtYB-ADH1t
Plasmid#65359PurposeTU for combinatorial construction of ?-carotene pathwayDepositorInsertTEF2p-ycrtYB-ADH1t
UseUnspecifiedTagsExpressionMutationPromoterAvailable sinceFeb. 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
POT2-TEF2p-ycrtE-ADH1t
Plasmid#65353PurposeTU for combinatorial construction of ?-carotene pathwayDepositorInsertTEF2p-ycrtE-ADH1t
UseUnspecifiedTagsExpressionMutationPromoterAvailable sinceFeb. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pUC-UAS1B16-TEF(1004)
Plasmid#44365DepositorInsertUAS1B16-TEF(1004) promoter
UseSynthetic BiologyTagsExpressionBacterialMutationN/APromoterUAS1B16-TEF(1004)Available sinceJuly 15, 2013AvailabilityIndustry, Academic Institutions, and Nonprofits -
pDSM-ab-Psptef1-NAT-Ttip1
Plasmid#127747PurposeYeast pathway position 2. NatMX transcription unit with the S. paradoxus TEF1 promoter and TIP1 terminator. Nourseothricin resistance.DepositorInsertnourseothricin N-acetyltransferase
UseSynthetic BiologyTagsExpressionYeastMutationPromoterPsptef1Available sinceNov. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pUC-UAS1B8-TEF(1004)
Plasmid#44366DepositorInsertUAS1B8-TEF(1004) promoter
UseSynthetic BiologyTagsExpressionBacterialMutationN/APromoterUAS1B8-TEF(1004)Available sinceJuly 15, 2013AvailabilityIndustry, Academic Institutions, and Nonprofits -
POT4-TEF2p-ycrtI-ADH1t
Plasmid#65356PurposeTU for combinatorial construction of ?-carotene pathwayDepositorInsertTEF2p-ycrtI-ADH1t
UseUnspecifiedTagsExpressionMutationPromoterAvailable sinceFeb. 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
pUC-UAS1B8-TEF(203)
Plasmid#44378DepositorInsertUAS1B8-TEF(203) promoter
UseSynthetic BiologyTagsExpressionBacterialMutationN/APromoterUAS1B8-TEF(203)Available sinceApril 18, 2013AvailabilityIndustry, Academic Institutions, and Nonprofits -
pUC-UAS1B8-TEF(804)
Plasmid#44368DepositorInsertUAS1B8-TEF(804) promoter
UseSynthetic BiologyTagsExpressionBacterialMutationN/APromoterUAS1B8-TEF(804)Available sinceApril 18, 2013AvailabilityIndustry, Academic Institutions, and Nonprofits -
pRS413-TEF1pr-SEC61-FAPoptim
Plasmid#221126PurposeFAP-tagged marker for the ER (FAP optimized for yeast expression)DepositorInsertSEC61-FAPoptim
UseTagsExpressionYeastMutationPromoterTEF1Available sinceApril 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRS415-TEF1pr-SEC63-FAPoptim
Plasmid#221116PurposeFAP tagged SEC63 (C-terminally tagged, optimized) under the Tef1 promoter with 2xMYC tagDepositorInsertFAPoptim-SEC63
UseTagsExpressionYeastMutationPromoterTEF1Available sinceApril 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRS415-TEF1pr-SEC61-FAPoptim
Plasmid#221115PurposeFAP tagged SEC61 (C-terminally tagged, optimized) under the Tef1 promoter with 2xMYC tagDepositorInsertSEC61-FAPoptim
UseTagsExpressionYeastMutationPromoterTEF1Available sinceApril 3, 2025AvailabilityAcademic Institutions and Nonprofits only