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pET22b_EcSSB_2
Plasmid#78204PurposeExpression of single-cysteine variant of single-stranded DNA-binding protein protein, EcSSB, as scaffold for ssDNA biosensorDepositorInsertssb (W88C)
UseTagsExpressionBacterialMutationW88C (please see depositor comment below)PromoterT7Available sinceJuly 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 RL3Sc
Plasmid#109365PurposeHuman rod opsin chimera with intracellular loop 3 from Scallop opsin with 1D4 tagDepositorInsertUseTags1D4ExpressionMammalianMutationPromoterCMVAvailable sinceMay 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 RL3Am
Plasmid#109364PurposeHuman rod opsin chimera with intracellular loop 3 from Amphioxus opsin 1 with 1D4 tagDepositorInsertRod opsin chimera with Amphioxus Opsin1 intracellular Loop 3 (RHO Human, Branchiostoma belcheri)
UseTags1D4ExpressionMammalianMutationPromoterCMVAvailable sinceMay 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
3xMS-TR-100-PURO HRD
Plasmid#207582Purposehomologous recombination donor for the insertion of the 3xMS2-tag at the endogenous locusDepositorInsert3xMS2-TR and 100 bp of downstream sequence followed by a PuroR and flanked by endogenous TR sequences
UseTags3xMS2ExpressionMammalianMutationPromoterSV40Available sinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
mCherry-Dyskerin
Plasmid#207577PurposeN-terminal mCherry-tag using SalI/EcoRIDepositorInsertgenerated by restriction cloning of gene into a modified pBABE-puro vector containing the N-terminal mCherrytag using SalI/EcoRI
UseTagsmCherryExpressionMammalianMutationPromoterLTRAvailable sinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
Halo-TCAB1 HRD
Plasmid#207535PurposeHomologous recombination donor to insert HaloTag at the N-terminus of the endogenous TCAB1 locus.DepositorInsertHaloTag with internal PuroR cassette flanked by human TCAB1 locus sequences
UseTagsHaloTagExpressionMammalianMutationPromoterEndogenousAvailable sinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
3xMS2 TR knockin sgRNA 1
Plasmid#207607PurposesgRNA for homology directed repair insertion of 3xMS2-TR-100-PURO into the endogenous TR locusDepositorInsertcgactcgcccggcagcgcac
UseTagsNoneExpressionMammalianMutationPromoterCMV and U6Available sinceApril 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
3xMS2 TR knockin sgRNA 2
Plasmid#207608PurposesgRNA 2 for homology directed repair insertion of 3xMS2-TR-100-PURO into the endogenous TR locusDepositorInsertacccccaaacctgactgact
UseTagsNoneExpressionMammalianMutationPromoterCMV and U6Available sinceApril 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-FLAG-TAPBPR-tapasinCT I261A
Plasmid#153476PurposeMammalian expression of FLAG-tagged TAPBPR with tapasin CT I261ADepositorInsertTAPBPR (TAPBPL Human)
UseTagsFLAGExpressionMammalianMutationC terminal tail of tapasin, I261APromoterCMVAvailable sinceJune 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-FLAG-TAPBPR-tapasinCT S333A
Plasmid#153477PurposeMammalian expression of FLAG-tagged TAPBPR with tapasin CT S333ADepositorInsertTAPBPR (TAPBPL Human)
UseTagsFLAGExpressionMammalianMutationC terminal tail of tapasin, S333APromoterCMVAvailable sinceJune 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-FLAG-Tapasin delta2step
Plasmid#153479PurposeMammalian expression of FLAG-tagged Tapasin delta2stepDepositorInsertTAPBP (TAPBP Human)
UseTagsFLAGExpressionMammalianMutationdeletion of aa.13-14, 16-17PromoterCMVAvailable sinceJune 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-FLAG-Tapasin delta3step
Plasmid#153480PurposeMammalian expression of FLAG-tagged Tapasin delta3stepDepositorInsertTAPBP (TAPBP Human)
UseTagsFLAGExpressionMammalianMutationdeletion of aa.12-14, 16-18PromoterCMVAvailable sinceJune 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-FLAG-Tapasin G15E
Plasmid#153481PurposeMammalian expression of FLAG-tagged Tapasin G15EDepositorInsertTAPBP (TAPBP Human)
UseTagsFLAGExpressionMammalianMutationG15EPromoterCMVAvailable sinceJune 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-FLAG-Tapasin G15L
Plasmid#153482PurposeMammalian expression of FLAG-tagged Tapasin G15LDepositorInsertTAPBP (TAPBP Human)
UseTagsFLAGExpressionMammalianMutationG15LPromoterCMVAvailable sinceJune 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-FLAG-Tapasin L18E
Plasmid#153483PurposeMammalian expression of FLAG-tagged Tapasin L18EDepositorInsertTAPBP (TAPBP Human)
UseTagsFLAGExpressionMammalianMutationL18EPromoterCMVAvailable sinceJune 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-FLAG-Tapasin L18G
Plasmid#153484PurposeMammalian expression of FLAG-tagged Tapasin L18GDepositorInsertTAPBP (TAPBP Human)
UseTagsFLAGExpressionMammalianMutationL18GPromoterCMVAvailable sinceJune 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-FLAG-Tapasin L18K
Plasmid#153485PurposeMammalian expression of FLAG-tagged Tapasin L18KDepositorInsertTAPBP (TAPBP Human)
UseTagsFLAGExpressionMammalianMutationL18KPromoterCMVAvailable sinceJune 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pETCON-Aga2p-TAPBPR-myc
Plasmid#153486PurposeYeast expression of Aga2p-TAPBPR-mycDepositorInsertTAPBPR (TAPBPL Human)
UseTagsAga2P and c-myc epitope tagExpressionYeastMutationPromoterGALAvailable sinceJune 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-FLAG-TAPBPR-tapasinCT
Plasmid#141309PurposeMammalian expression of FLAG-tagged TAPBPR with tapasin CTDepositorInsertTAPBPR (TAPBPL Human)
UseTagsFLAGExpressionMammalianMutationC terminal tail of tapasinPromoterCMVAvailable sinceJune 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-FLAG-TAPBPR-tapasinCT E102A
Plasmid#153473PurposeMammalian expression of FLAG-tagged TAPBPR with tapasin CT E102ADepositorInsertTAPBPR (TAPBPL Human)
UseTagsFLAGExpressionMammalianMutationC terminal tail of tapasin, E102APromoterCMVAvailable sinceJune 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-FLAG-TAPBPR-tapasinCT M122A
Plasmid#153474PurposeMammalian expression of FLAG-tagged TAPBPR with tapasin CT M122ADepositorInsertTAPBPR (TAPBPL Human)
UseTagsFLAGExpressionMammalianMutationC terminal tail of tapasin, M122APromoterCMVAvailable sinceJune 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-FLAG-TAPBPR-tapasinCT G212A
Plasmid#153475PurposeMammalian expression of FLAG-tagged TAPBPR with tapasin CT G212ADepositorInsertTAPBPR (TAPBPL Human)
UseTagsFLAGExpressionMammalianMutationC terminal tail of tapasin, G212APromoterCMVAvailable sinceJune 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CaMKII-jGCaMP7f-WPRE
Plasmid#203848PurposeExpression of GCaMP7f in CaMKII expressing cellsDepositorInsertjGCaMP7f
UseAAVTagsExpressionMutationPromoterAvailable sinceAug. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSG020
Plasmid#126503PurposePseudomonas aeruginosa disaggregase ClpG(GI) cloned as ClpG(GI)-6xHisDepositorInsertdisaggregase ClpGgi with 6xHis-tag
UseTagsC-terminal 6xHisExpressionBacterialMutationPromoterPBADAvailable sinceAug. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 hML23Cm6
Plasmid#109368PurposeHuman melanopsin chimera with intracellular loops 2-3 and C-terminus from mGluR6DepositorUseTags1D4ExpressionMammalianMutationPromoterCMVAvailable sinceMay 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-MR1 (M50P)
Plasmid#178651PurposeMammalian expression of myc-tagged MR1 (M50P)DepositorInsertMR1 (MR1 Human)
UseTagsExtracellular c-myc epitope ta and Signal peptide…ExpressionMammalianMutationM50PPromoterCMVAvailable sinceJuly 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCEP4 MR1 (H83P)
Plasmid#178652PurposeMammalian expression of myc-tagged MR1 (H83P)DepositorInsertMR1 (MR1 Human)
UseTagsExtracellular c-myc epitope ta and Signal peptide…ExpressionMammalianMutationH83PPromoterCMVAvailable sinceJuly 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCEP4 MR1 (Q153P)
Plasmid#178653PurposeMammalian expression of myc-tagged MR1 (Q153P)DepositorInsertMR1 (MR1 Human)
UseTagsExtracellular c-myc epitope ta and Signal peptide…ExpressionMammalianMutationQ153PPromoterCMVAvailable sinceJuly 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-FLAG-Tapasin-TAPBPR 22-35
Plasmid#153478PurposeMammalian expression of FLAG-tagged Tapasin with TAPBPR a.a. 22-35DepositorInsertTAPBP (TAPBP Human)
UseTagsFLAGExpressionMammalianMutationTapasin a.a.10-20 replaced with TAPBPR a.a. 22-35PromoterCMVAvailable sinceJune 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-FLAG-TAPBPR-tapasinCT deltaALAS
Plasmid#153471PurposeMammalian expression of FLAG-tagged TAPBPR with tapasin CT deltaALASDepositorInsertTAPBPR (TAPBPL Human)
UseTagsFLAGExpressionMammalianMutationC terminal tail of tapasin, deletion of amino aci…PromoterCMVAvailable sinceJune 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-FLAG-TAPBPR-tapasinCT delta24-36
Plasmid#153472PurposeMammalian expression of FLAG-tagged TAPBPR with tapasin CT delta24-36DepositorInsertTAPBPR (TAPBPL Human)
UseTagsFLAGExpressionMammalianMutationC terminal tail of tapasin, deletion of amino aci…PromoterCMVAvailable sinceJune 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
MTK3_015
Plasmid#123730PurposeEncodes Gi-coupled hM4D as a Type 3 part to be used in the MTK systemDepositorInserthM4D(Gi)
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
B1-INTEGRIN WT
Plasmid#195215PurposeMammalian expression of mCherry tagged B1 Integrin. Wild-type.DepositorInsertITGB1 (ITGB1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-TAPBPR-TM
Plasmid#178645PurposeMammalian expression of FLAG-tagged TAPBPR with MHC TMDepositorInsertTAPBPR (TAPBPL Human)
UseTagsFLAG and Signal/leader sequence from HLA class I …ExpressionMammalianMutationswitched transmembrane domain with that of MHC-IPromoterCMVAvailable sinceJuly 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCbs FlagOmomyc
Plasmid#113168PurposeExpression of FLAG tagged Omomyc in mammalian cellsDepositorInsertOmomyc (MYC Human)
UseTagsFLAGExpressionMammalianMutationE57T, E64I, R70Q, R71NPromoterAvailable sinceAug. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAdTrack-CMV FLAG Rev-Erb alpha
Plasmid#22745DepositorInsertRev Erb alpha (NR1D1 Human)
UseAdenoviralTagsFlagExpressionMammalianMutationPromoterAvailable sinceJan. 6, 2010AvailabilityAcademic Institutions and Nonprofits only
