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We narrowed to 1,499 results for: U6 promoter

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Showing: 121 - 160 of 1499 results

  20. pShuttle-CMV-GFP U6promoter

    Plasmid
    #128049
    Purpose
    Adenoviral shuttle vector for shRNA expression from U6 promoter with GFP marker
    Depositor
    Oskar Laur
    Type
    Empty backbone
    Use
    Adenoviral
    Expression
    Mammalian
    Available Since
    Aug. 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    128049-plasmid-map-sequence-id-255046

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  24. pUC19-U6-BsmBI_cassette-St3Cas9-sgRNA (KAC27)

    Plasmid
    #133792
    Purpose
    U6 promoter sgRNA entry vector used for all St3Cas9 sgRNAs (clone spacer oligos into BsmBI cassette) - similar to sgRNA architecture from Muller et al. Molecular Therapy 2015
    Depositor
    Joseph Bondy-Denomy, Benjamin Kleinstiver
    Article
    Mahendra et al Nat Microbiol. 2020 Apr;5(4):620-629. do
    Insert
    St3Cas9 sgRNA entry vector
    Expression
    Mammalian
    Mutation
    sgRNA architecture from Muller et al. Molecular T…
    Promoter
    U6
    Available Since
    March 30, 2020
    Availability
    Academic Institutions and Nonprofits only
    133792-plasmid-map-sequence-id-268589

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  25. pUC19-U6-BsmBI_cassette-CjeCas9-sgRNA (KAC482)

    Plasmid
    #133793
    Purpose
    U6 promoter sgRNA entry vector used for all CjeCas9 sgRNAs (clone spacer oligos into BsmBI cassette) - similar to V2-TGAA linker sgRNA architecture from Kim et al. Nature Communications 2017
    Depositor
    Joseph Bondy-Denomy, Benjamin Kleinstiver
    Article
    Mahendra et al Nat Microbiol. 2020 Apr;5(4):620-629. do
    Insert
    CjeCas9 sgRNA entry vector
    Expression
    Mammalian
    Mutation
    V2-TGAA linker sgRNA architecture from Kim et al.…
    Promoter
    U6
    Available Since
    March 30, 2020
    Availability
    Academic Institutions and Nonprofits only
    133793-plasmid-map-sequence-id-268077

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  26. pUC19-U6-BsmBI_cassette-St1Cas9-sgRNA (KAC14)

    Plasmid
    #133791
    Purpose
    U6 promoter sgRNA entry vector used for all St1Cas9 sgRNAs (clone spacer oligos into BsmBI cassette) - similar to V1 sgRNA architecture from Carter et al. biorxiv 2018
    Depositor
    Joseph Bondy-Denomy, Benjamin Kleinstiver
    Article
    Mahendra et al Nat Microbiol. 2020 Apr;5(4):620-629. do
    Insert
    St1Cas9 sgRNA entry vector
    Expression
    Mammalian
    Mutation
    V1 sgRNA architecture from Carter et al. biorxiv …
    Promoter
    U6
    Available Since
    March 30, 2020
    Availability
    Academic Institutions and Nonprofits only
    133791-plasmid-map-sequence-id-268588

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  27. pLenti-U6-sgCTG-CAG-Cas9D10A nickase-Blast

    Plasmid
    #216733
    Purpose
    Expresses SpCas9-D10A nickase under a CAG promoter together with a blasticidin resistance gene, along with a U6 promoter driving the sgCTG (target sequence: (CUG)6). Suitable for lentivirus packaging.
    Depositor
    Vincent Dion
    Article
    Alvaro et al bioRxiv
    Insert
    Cas9-D10A nickase and sgCTG
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Mutation
    D10A
    Promoter
    U6 and CAG
    Available Since
    April 24, 2024
    Availability
    Academic Institutions and Nonprofits only
    216733-plasmid-map-sequence-id-430318

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  28. pLV(gRNA)-CMV-eGFP-U6(sgCTG)

    Plasmid
    #216732
    Purpose
    Contains a eGFP gene along with a U6 promoter driving the sgCTG (target sequence: (CUG)6). Used with the HD iPSC-derived astrocytes
    Depositor
    Vincent Dion
    Article
    Alvaro et al bioRxiv
    Insert
    sgCTG lentiviral guide RNA with eGFP tag
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    CMV and U6
    Available Since
    April 24, 2024
    Availability
    Academic Institutions and Nonprofits only
    216732-plasmid-map-sequence-id-430316

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  30. (pRZ318) AAV: U6-gRNA(FLEX)-EF1a-mScarlet

    Plasmid
    #254587
    Purpose
    AAV backbone expressing a U6-driven sgRNA with SapI spacer and 10x FLEX preferred scaffold and mScarlet from the EF1a promoter
    Depositor
    Jesse Engreitz
    Insert
    sgRNA with SapI spacer
    Use
    AAV
    Available Since
    April 8, 2026
    Availability
    Academic Institutions and Nonprofits only
    254587-plasmid-map-sequence-id-505743

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  31. (pRZ320) AAV: U6-gRNA(FLEX)-EF1a-ZsGreen

    Plasmid
    #254588
    Purpose
    AAV backbone expressing a U6-driven sgRNA with SapI spacer and 10x FLEX preferred scaffold and ZsGreen from the EF1a promoter
    Depositor
    Jesse Engreitz
    Insert
    sgRNA with SapI spacer
    Use
    AAV
    Available Since
    April 8, 2026
    Availability
    Academic Institutions and Nonprofits only
    254588-plasmid-map-sequence-id-505741

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  32. (pRZ76) AAV: U6-gRNA(cs1)-EF1a-mCherry

    Plasmid
    #254583
    Purpose
    AAV backbone expressing a U6-driven sgRNA with SapI spacer and capture sequence in scaffold and mCherry from the EF1a promoter
    Depositor
    Jesse Engreitz
    Insert
    sgRNA with SapI spacer
    Use
    AAV
    Available Since
    April 8, 2026
    Availability
    Academic Institutions and Nonprofits only
    254583-plasmid-map-sequence-id-505738

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  35. pUC19-U6-[BsmBI_pegRNA_entry]-tevopreQ1-term (LM1138)

    Plasmid
    #223137
    Purpose
    pU6 entry plamid for cloning prime editor tevopreQ1 epegRNAs (requires BsmBI or Esp3I digest and ligation of duplexed oligos)
    Depositor
    Benjamin Kleinstiver
    Article
    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586
    Insert
    etevopreQ1 entry plasmid backbone for epegRNA expression via human U6 promoter
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    U6
    Available Since
    April 23, 2025
    Availability
    Academic Institutions and Nonprofits only
    223137-plasmid-map-sequence-id-447707

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  36. pKLV2-U6(gCD44v2)-EF1a-BFP-Puro-Cas9(FZ)

    Plasmid
    #117134
    Purpose
    All-in-one expression vector for Cas9 and gRNA against CD44
    Depositor
    Kosuke Yusa
    Insert
    gCD44 v2 (Cd44 Synthetic)
    Use
    CRISPR and Lentiviral
    Mutation
    Deleted BbsI site within WPRE, modified BFP
    Promoter
    human U6 promoter, human EF1a promoter
    Available Since
    April 26, 2019
    Availability
    Academic Institutions and Nonprofits only
    117134-plasmid-map-sequence-id-226805

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  38. pJRH-1345 U6-B2M sgRNA Gag-Cas9 v2

    Plasmid
    #201916
    Purpose
    Cas9-EDV production plasmid. Expresses the v2 Gag-Cas9 polypeptide (CAG promoter) and B2M sgRNA (human U6 promoter, 5’-GAGTAGCGCGAGCACAGCTA)
    Depositor
    Jennifer Doudna
    Article
    Hamilton et al Nat Biotechnol. 2024 Jan 11. doi: 10.103
    Inserts
    Gag-Cas9 v2
    B2M sgRNA (Target-specific sequence: 5'- GAGTAGCGCGAGCACAGCTA)
    Expression
    Mammalian
    Promoter
    CAG and Human U6
    Available Since
    Aug. 4, 2023
    Availability
    Academic Institutions and Nonprofits only
    201916-plasmid-map-sequence-id-403306

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Showing: 121 - 160 of 1499 results