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We narrowed to 292 results for: bicistronic

Showing: 121 - 160 of 292 results

  2. CMV:AsCpf1-2A-GFP-U6-tdTomato-sg

    Plasmid
    #194722
    Purpose
    Bicistronic vector expressing CMV promoter driven AsCpf1, and U6 promoter driven guide RNA that target tdTomato
    Depositor
    Rudolf Jaenisch
    Article
    Haiting et al bioRxiv
    Insert
    AsCpf1
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 10, 2023
    Availability
    Academic Institutions and Nonprofits only
    194722-plasmid-map-sequence-id-381295

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  3. CMV:AsCpf1-2A-GFP-U6-GFP-sg

    Plasmid
    #194717
    Purpose
    Bicistronic vector expressing CMV promoter driven AsCpf1, and U6 promoter driven guide RNA that target GFP
    Depositor
    Rudolf Jaenisch
    Article
    Haiting et al bioRxiv
    Insert
    AsCpf1
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    194717-plasmid-map-sequence-id-381222

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  4. pBHSTC104

    Plasmid
    #124356
    Purpose
    Plasmid expressing N- and C-terminal split fragments of gp41-1 intein inserted into CcdA antitoxin
    Depositor
    Hideo Iwai
    Article
    Beyer et al Chembiochem. 2019 Apr 8. doi: 10.1002/cb
    Insert
    gp41-1 N-intein and C-intein (bicistronic)
    Use
    Tags
    CcdA-C (SFAD)
    Expression
    Bacterial
    Mutation
    Promoter
    STC
    Available since
    April 18, 2019
    Availability
    Academic Institutions and Nonprofits only
    124356-plasmid-map-sequence-id-242355

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  7. CMV:AsCpf1-2A-GFP-U6-AAVS1-sg

    Plasmid
    #194716
    Purpose
    Bicistronic vector expressing CMV promoter driven AsCpf1, and U6 promoter driven guide RNA that target hAAVS1 locus
    Depositor
    Rudolf Jaenisch
    Article
    Haiting et al bioRxiv
    Insert
    AsCpf1 (AAVS1 Acidaminococcus sp.)
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    194716-plasmid-map-sequence-id-381220

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  10. pMIG-w

    Plasmid
    #12282
    Purpose
    a bicistronic IRES-GFP containing vector which is a variant of pMIG that contains a posttranscriptional regulatory element from the woodchuck hepatitis virus
    Depositor
    Luk Parijs
    Article
    Refaeli et al J Exp Med. 2002 Oct 7. 196(7):999-1005.
    Type
    Empty backbone
    Use
    Retroviral
    Tags
    IRES GFP
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    July 28, 2006
    Availability
    Academic Institutions and Nonprofits only

  12. pSEVA3b61

    Plasmid
    #58310
    Purpose
    Shuttle vector: pUC ori, pTHT15 ori for use in E. coli/B. subtilis. Cannot modify orientation of cat gene; promoter too weak in E. coli, probably transcribed via repB promoter in bicistronic manner.
    Depositor
    Tom Ellis
    Article
    Wright et al ACS Synth Biol. 2014 May 13.
    Type
    Empty backbone
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    July 29, 2015
    Availability
    Academic Institutions and Nonprofits only
    58310-plasmid-map-sequence-id-89822

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  15. pNO183

    Plasmid
    #101068
    Purpose
    ppsR2-bphP1 bicistronic operon plasmid
    Depositor
    Jeffrey Tabor
    Article
    Ong et al ACS Synth Biol. 2017 Nov 1. doi: 10.1021
    Inserts
    ppsR2 (ppsR2 Rhodopseudomonas palustris CGA009)
    bphP1
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    insert guanine at position 430 to fix frameshift …
    Promoter
    Available since
    Nov. 10, 2017
    Availability
    Academic Institutions and Nonprofits only
    101068-plasmid-map-sequence-id-194033

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  16. COVID-SARS2 NSP16/10

    Plasmid
    #159615
    Purpose
    Bacterial co-expression vector fo Covid-SARS2 NSP16 and NSP10
    Depositor
    Opher Gileadi
    Inserts
    Covid-SARS2 Nsp16
    Covid-SARS2 Nsp10
    Use
    Tags
    His6-Zb-TEV and none
    Expression
    Bacterial
    Mutation
    Codon-optimized for E. coli expression
    Promoter
    (Bicistronic) and T7 - LacO
    Available since
    Sept. 15, 2020
    Availability
    Academic Institutions and Nonprofits only
    159615-plasmid-map-sequence-id-311066

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  18. pLP-hSox2-ires-gfp

    Plasmid
    #49391
    Purpose
    mammalian bicistronic expression of human Sox2 and EGFP
    Depositor
    Randall McKinnon
    Insert
    Sox2 (SOX2 Human)
    Use
    Cre/Lox; Acceptor vector
    Tags
    6xHN, EGFP, and IRES
    Expression
    Mammalian
    Mutation
    TGA (stop) to CCA (Pro) at nt 1027 with respect t…
    Promoter
    CMV
    Available since
    Dec. 2, 2013
    Availability
    Academic Institutions and Nonprofits only

  20. pHBSTC146

    Plasmid
    #124357
    Purpose
    Plasmid expressing inactive N- and C-terminal split fragments of gp41-1 intein inserted into CcdA antitoxin
    Depositor
    Hideo Iwai
    Article
    Beyer et al Chembiochem. 2019 Apr 8. doi: 10.1002/cb
    Insert
    gp41-1 N-intein (C1G) and C-intein (bicistronic)
    Use
    Tags
    CcdA-C (SFAD)
    Expression
    Bacterial
    Mutation
    C1G
    Promoter
    STC
    Available since
    April 16, 2019
    Availability
    Academic Institutions and Nonprofits only
    124357-plasmid-map-sequence-id-241737

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  22. pCAGIG

    Plasmid
    #11159
    Purpose
    Mammalian expression vector for cloning and expressing your gene on a bicistronic mRNA also encoding IRES-GFP
    Depositor
    Connie Cepko
    Article
    Matsuda et al Proc Natl Acad Sci U S A. 2004 Jan 6. 10
    Has Service
    Cloning Grade DNA
    Insert
    IRES-EGFP
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    April 13, 2006
    Availability
    Academic Institutions and Nonprofits only
    11159-plasmid-map-sequence-id-275611

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  25. pZH509

    Plasmid
    #102664
    Purpose
    Tetracycline inducible expression of GFPmut2 using bicistronic autoregulation; strong ribosome binding site; GFPmut2 ORF can be replaced with gene of interest by isothermal assembly
    Depositor
    Zach Hensel
    Article
    Hensel et al PLoS One. 2017 Oct 30;12(10):e0187259. d
    Insert
    GFPmut2
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Dec. 1, 2017
    Availability
    Academic Institutions and Nonprofits only
    102664-plasmid-map-sequence-id-199389

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  26. pZH512

    Plasmid
    #102666
    Purpose
    Tetracycline inducible expression of GFPmut2 using bicistronic autoregulation; moderate ribosome binding site; GFPmut2 ORF can be replaced with gene of interest by isothermal assembly
    Depositor
    Zach Hensel
    Article
    Hensel et al PLoS One. 2017 Oct 30;12(10):e0187259. d
    Insert
    GFPmut2
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Dec. 1, 2017
    Availability
    Academic Institutions and Nonprofits only
    102666-plasmid-map-sequence-id-199387

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  27. pZH511

    Plasmid
    #102665
    Purpose
    Tetracycline inducible expression of GFPmut2 using bicistronic autoregulation; weak ribosome binding site; GFPmut2 ORF can be replaced with gene of interest by isothermal assembly
    Depositor
    Zach Hensel
    Article
    Hensel et al PLoS One. 2017 Oct 30;12(10):e0187259. d
    Insert
    GFPmut2
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Dec. 11, 2017
    Availability
    Academic Institutions and Nonprofits only
    102665-plasmid-map-sequence-id-199381

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  30. CMV:AsCpf1-2A-GFP-U6-INS-sg

    Plasmid
    #194719
    Purpose
    Bicistronic vector expressing CMV promoter driven AsCpf1, and U6 promoter driven guide RNA that target hINS
    Depositor
    Rudolf Jaenisch
    Article
    Haiting et al bioRxiv
    Insert
    AsCpf1 (INS Acidaminococcus sp.)
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    194719-plasmid-map-sequence-id-381224

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  31. CMV:AsCpf1-2A-GFP-U6-MAFB-sg

    Plasmid
    #194718
    Purpose
    Bicistronic vector expressing CMV promoter driven AsCpf1, and U6 promoter driven guide RNA that target hMAFB
    Depositor
    Rudolf Jaenisch
    Article
    Haiting et al bioRxiv
    Insert
    AsCpf1 (MAFB Acidaminococcus sp.)
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    194718-plasmid-map-sequence-id-381223

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  32. pT5T_Rev7_1-211_SA112_KVK_Rev3_1873-1898

    Plasmid
    #105635
    Purpose
    Expresses mouse monomeric Rev7 and fragment of Rev3 protein (amino acids 1873-1898) from bicistronic plasmid
    Depositor
    Thanos Halazonetis
    Inserts
    Rev7 (Mad2l2 Mouse)
    Rev3l (REV3L Human)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Mutations: F11S, G12A, V132K, C133V, A135K
    Promoter
    Available since
    March 14, 2022
    Availability
    Academic Institutions and Nonprofits only
    105635-plasmid-map-sequence-id-203205

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Showing: 121 - 160 of 292 results