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We narrowed to 571 results for: GI

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Showing: 151 - 200 of 571 results

  5. PEA1

    Plasmid
    #176527
    Purpose
    Delivers all prime editing nickase components in a single plasmid
    Depositor
    Paul Thomas
    Article
    Adikusuma et al Nucleic Acids Res. 2021 Sep 17. pii: 637
    Insert
    CbH-Cas9(H840A)-RT, hU6-pegRNA (Bbs1 gate), hU6-sgRNA (Bbs1 gate)
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    CbH for Cas9, hU6 for gRNAs
    Available Since
    Dec. 16, 2021
    Availability
    Academic Institutions and Nonprofits only
    176527-plasmid-map-sequence-id-350558

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  19. pcDNA3.1_mutNSDHL

    Plasmid
    #136428
    Purpose
    Expresses NSDHL harboring all currently known mutations within exons 4 and 6 associated with CHILD syndrome.
    Depositor
    Antonio Amelio
    Article
    Getz et al Oral Surg Oral Med Oral Pathol Oral Radi
    Insert
    human NSDHL with mutations in exon 4 and exon 6 (NSDHL Human, Synthetic)
    Tags
    V5-His tag
    Expression
    Mammalian
    Mutation
    C>T = A105V; G>C = A182P; G>A = R199H; G…
    Promoter
    CMV
    Available Since
    March 5, 2020
    Availability
    Academic Institutions and Nonprofits only
    136428-plasmid-map-sequence-id-273784

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  24. PEA1-GFP

    Plasmid
    #171993
    Purpose
    Delivers all prime editing nickase components in a single, GFP selectable plasmid
    Depositor
    Paul Thomas
    Article
    Adikusuma et al Nucleic Acids Res. 2021 Sep 17. pii: 637
    Insert
    CbH-Cas9(H840A)-RT-T2A-GFP, hU6-pegRNA (Bbs1 gate), hU6-sgRNA (Bbs1 gate)
    Expression
    Mammalian
    Promoter
    CMV for Cas9, U6 for gRNAs
    Available Since
    Aug. 20, 2021
    Availability
    Academic Institutions and Nonprofits only
    171993-plasmid-map-sequence-id-340552

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  25. PEA1-Puro

    Plasmid
    #171991
    Purpose
    Delivers all prime editing nickase components in a single, puromycin selectable plasmid
    Depositor
    Paul Thomas
    Article
    Adikusuma et al Nucleic Acids Res. 2021 Sep 17. pii: 637
    Insert
    CbH-Cas9(H840A)-RT-T2A-Puro, hU6-pegRNA (Bbs1 gate), hU6-sgRNA (Bbs1 gate)
    Expression
    Mammalian
    Promoter
    CMV for Cas9, U6 for gRNAs
    Available Since
    July 21, 2021
    Availability
    Academic Institutions and Nonprofits only
    171991-plasmid-map-sequence-id-338127

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  27. PDG459 V3

    Plasmid
    #226958
    Purpose
    CBh-SpCas9-2A-Puro, and 2X hU6-sgRNA (Sp) with BbsI golden gate cloning backbone dual gRNAs. sgRNA uses 3TC scaffold for increased sgRNA expression.
    Depositor
    Paul Thomas
    Article
    Chey et al BMC Genomics. 2025 Feb 12;26(1):138. doi
    Type
    Empty backbone
    Use
    CRISPR
    Expression
    Mammalian
    Available Since
    Oct. 30, 2024
    Availability
    Academic Institutions and Nonprofits only
    226958-plasmid-map-sequence-id-487610

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  29. eMscL WT

    Plasmid
    #107454
    Purpose
    eMscL WT is optimized for mammalian rodent neuronal expression to the plasma membrane through a neuron-specific promoter and a voltage-gated channel targeting motif
    Depositor
    Francesco Difato
    Article
    Soloperto et al J Cell Sci. 2018 Jan 29. pii: jcs.210393
    Insert
    bacterial mechanosensitive ion channel of large conductance (mscL Escherichia Coli)
    Use
    Adeno-associated viral
    Tags
    Kir2.1 ER export signal (FCYENEV) and tdTomato fl…
    Expression
    Mammalian
    Promoter
    human synapsin 1
    Available Since
    April 2, 2018
    Availability
    Academic Institutions and Nonprofits only
    107454-plasmid-map-sequence-id-207518

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  31. SaCas9v3-Puro

    Plasmid
    #178813
    Purpose
    SaCas9 with 2A-Puro, and a cloning backbone for sgRNA. sgRNA scaffold seqeuence has been modified for increased sgRNA expression (v3).
    Depositor
    Paul Thomas
    Article
    Chey et al BMC Genomics. 2025 Feb 12;26(1):138. doi
    Type
    Empty backbone
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    Cbh (Cas9-2A Puro) and U6 (gRNA)
    Available Since
    Aug. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    178813-plasmid-map-sequence-id-354841

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  33. PDG458 V3

    Plasmid
    #226959
    Purpose
    CBh-SpCas9-2A-GFP, and 2X hU6-sgRNA (Sp) with BbsI golden gate cloning backbone dual gRNAs. sgRNA uses 3TC scaffold for increased sgRNA expression.
    Depositor
    Paul Thomas
    Article
    Chey et al BMC Genomics. 2025 Feb 12;26(1):138. doi
    Type
    Empty backbone
    Use
    CRISPR
    Expression
    Mammalian
    Available Since
    Oct. 30, 2024
    Availability
    Academic Institutions and Nonprofits only
    226959-plasmid-map-sequence-id-450819

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  35. eMscL G22S

    Plasmid
    #107455
    Purpose
    eMscL G22S is optimized for mammalian rodent neuronal expression to the plasma membrane through a neuron-specific promoter and a voltage-gated channel targeting motif
    Depositor
    Francesco Difato
    Article
    Soloperto et al J Cell Sci. 2018 Jan 29. pii: jcs.210393
    Insert
    bacterial mechanosensitive ion channel of large conductance (mscL Escherichia coli)
    Use
    Adeno-associated viral
    Tags
    Kir2.1 ER export signal (FCYENEV) and tdTomato fl…
    Expression
    Mammalian
    Mutation
    Bearing a glycine to serine substitution at posit…
    Promoter
    human synapsin 1
    Available Since
    April 4, 2018
    Availability
    Academic Institutions and Nonprofits only
    107455-plasmid-map-sequence-id-207549

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  36. PX458 V3

    Plasmid
    #226957
    Purpose
    CBh-SpCas9-2A-GFP, and hU6-sgRNA (Sp) with a BbsI golden gate cloning backbone for sgRNA. sgRNA uses 3TC scaffold for increased sgRNA expression.
    Depositor
    Paul Thomas
    Article
    Chey et al BMC Genomics. 2025 Feb 12;26(1):138. doi
    Type
    Empty backbone
    Use
    CRISPR
    Expression
    Mammalian
    Available Since
    Oct. 30, 2024
    Availability
    Academic Institutions and Nonprofits only
    226957-plasmid-map-sequence-id-450777

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  37. MultiMate-Rainbow

    Plasmid
    #206252
    Purpose
    Expression of 7 fluorescently labelled proteins in mammalian cells. Can be used to generate recombinant baculovirus particles.
    Depositor
    Imre Berger
    Article
    Aulicino et al Nucleic Acids Res. 2022 Jul 22;50(13):77
    Insert
    H2B (human); Actin, Tubulin (B. taurus); mito mCherry, GST mTagBFP1 (Synthetic); CyOFP1 (E. quadricolor); Ctnnb1 (mouse)
    Use
    Recombinant baculovirus production (bac-to-bac)
    Tags
    iRFP713
    Expression
    Mammalian
    Available Since
    Sept. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    206252-plasmid-map-sequence-id-406651

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  49. MultiMate 5 colors

    Plasmid
    #206266
    Purpose
    Expression of 5 fluorescently labelled proteins in mammalian cells. Can be used as a CRE-donor (MultiBac system)
    Depositor
    Imre Berger
    Article
    Aulicino et al Nucleic Acids Res. 2022 Jul 22;50(13):77
    Insert
    H2B (H. Sapiens), Actin and Tubulin (B. taurus), mito mCherry (Synthetic), CyOFP1 (E. quadricolor)
    Use
    Recombinant baculovirus production, cre acceptor…
    Expression
    Mammalian
    Available Since
    Sept. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    206266-plasmid-map-sequence-id-406652

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Showing: 151 - 200 of 571 results