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Showing: 151 - 200 of 218 results

  1. phage UbiC NLS HA stdPCP stdGFP

    Plasmid
    #104099
    Purpose
    Lentiviral vector expressing synonymous tandem PP7 coat protein fused to synonymous tandem GFP. Used to label reporter mRNAs.
    Depositor
    Jeffrey Chao
    Article
    Horvathova et al Mol Cell. 2017 Nov 2;68(3):615-625.e9. d
    Insert
    stdPCP-stdGFP
    Use
    Lentiviral
    Tags
    N-terminal NLS, Two synonymous copies of GFP (C-t…
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Dec. 20, 2017
    Availability
    Academic Institutions and Nonprofits only
    104099-plasmid-map-sequence-id-200989

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  3. pMV_AA091

    Plasmid
    #216106
    Purpose
    CRISPRa, gRNA expression with modified trRNA to recruit activators via PP7 tags (guide only)
    Depositor
    John Doench
    Article
    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi
    Insert
    gRNA expression with modified trRNA to recruit activators via PP7 tags
    Use
    CRISPR and Lentiviral; Assembled vector
    Tags
    Expression
    Mutation
    Promoter
    Available since
    April 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    216106-plasmid-map-sequence-id-431410

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  5. pAAV-U6-racRNA-A

    Plasmid
    #200824
    Purpose
    AAV transfer plasmid encoding a circularized RNA barcode A under U6 promoter and a subcellular localization protein binder under hSyn promoter
    Depositor
    Xiao Wang
    Article
    Shi et al Nature. 2023 Oct;622(7983):552-561. doi:
    Inserts
    U6-racRNA
    V5-PP7cp-M9-NES-T2A-Flag-PP7cp-Far
    Use
    AAV
    Tags
    C-Far, Flag, and V5
    Expression
    Mammalian
    Mutation
    Promoter
    hSyn
    Available since
    Sept. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    200824-plasmid-map-sequence-id-396714

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  6. pAAV-U6-racRNA-B

    Plasmid
    #200825
    Purpose
    AAV transfer plasmid encoding a circularized RNA barcode B under U6 promoter and a subcellular localization protein binder under hSyn promoter
    Depositor
    Xiao Wang
    Article
    Shi et al Nature. 2023 Oct;622(7983):552-561. doi:
    Inserts
    U6-racRNA
    V5-PP7cp-M9-NES-T2A-Flag-PP7cp-Far
    Use
    AAV
    Tags
    C-Far, Flag, and V5
    Expression
    Mammalian
    Mutation
    Promoter
    hSyn
    Available since
    Sept. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    200825-plasmid-map-sequence-id-396771

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  7. pAAV-U6-racRNA-C

    Plasmid
    #200826
    Purpose
    AAV transfer plasmid encoding a circularized RNA barcode C under U6 promoter and a subcellular localization protein binder under hSyn promoter
    Depositor
    Xiao Wang
    Article
    Shi et al Nature. 2023 Oct;622(7983):552-561. doi:
    Inserts
    U6-racRNA
    V5-PP7cp-M9-NES-T2A-Flag-PP7cp-Far
    Use
    AAV
    Tags
    C-Far, Flag, and V5
    Expression
    Mammalian
    Mutation
    Promoter
    hSyn
    Available since
    Sept. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    200826-plasmid-map-sequence-id-396715

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  8. pAAV-U6-racRNA-D

    Plasmid
    #200827
    Purpose
    AAV transfer plasmid encoding a circularized RNA barcode D under U6 promoter and a subcellular localization protein binder under hSyn promoter
    Depositor
    Xiao Wang
    Article
    Shi et al Nature. 2023 Oct;622(7983):552-561. doi:
    Inserts
    U6-racRNA
    V5-PP7cp-M9-NES-T2A-Flag-PP7cp-Far
    Use
    AAV
    Tags
    C-Far, Flag, and V5
    Expression
    Mammalian
    Mutation
    Promoter
    hSyn
    Available since
    Sept. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    200827-plasmid-map-sequence-id-396773

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  14. pSP569

    Plasmid
    #139499
    Purpose
    Repair DNA plasmid to insert PP7 stem loops in the endogenous STL1 locus after double strand break in S. cerevisiae
    Depositor
    Serge Pelet
    Article
    Wosika et al Nat Commun. 2020 Jun 23;11(1):3171. doi:
    Insert
    pSTL1 24xPP7sl STL1 100 - 600
    Use
    Tags
    Expression
    Yeast
    Mutation
    WT
    Promoter
    pSTL1
    Available since
    June 12, 2020
    Availability
    Academic Institutions and Nonprofits only
    139499-plasmid-map-sequence-id-292017

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  24. pSB1C3_YPet-PCP (y-plasmid)

    Plasmid
    #127637
    Purpose
    Encodes a fluorecent protein with an RNA binding peptide: YPet-PCP.Expression with constitutive E. coli RNAP promoter (J23106), ribozyme RiboJ and RBS BBa_B0034.
    Depositor
    Friedrich Simmel
    Article
    Schwarz-Schilling et al Nano Lett. 2018 Apr 11;18(4):2650-2657.
    Insert
    YPet
    Use
    Synthetic Biology
    Tags
    RNA binding peptide: PP7 coat protein (PCP)
    Expression
    Bacterial
    Mutation
    Promoter
    pJ21306
    Available since
    July 29, 2019
    Availability
    Academic Institutions and Nonprofits only
    127637-plasmid-map-sequence-id-251705

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  27. pCasper4-g-osk-TRICK

    Plasmid
    #71244
    Purpose
    oskar translation biosensor with 12x copies of modified PP7 stem loops and 6xMS2 stem loops. . full genomic region, used for P element-mediated germline transformation in Drosophila.
    Depositor
    Anne Ephrussi
    Article
    Halstead et al Science. 2015 Mar 20;347(6228):1367-671.
    Insert
    oskar (osk Fly)
    Use
    P element-mediated germline transformation
    Tags
    12xPP7 and 6xMS2
    Expression
    Insect
    Mutation
    Promoter
    osk
    Available since
    Dec. 9, 2015
    Availability
    Academic Institutions and Nonprofits only
    71244-plasmid-map-sequence-id-127267

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  34. pHMG20B.1.0-gDNA

    Plasmid
    #132449
    Purpose
    CRISPR/Cas9 plasmid to create GFP fusion proteins
    Depositor
    Kevin White
    Insert
    HMG20B (HMG20B Human)
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 17, 2019
    Availability
    Academic Institutions and Nonprofits only
    132449-plasmid-map-sequence-id-266806

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  39. AA022

    Plasmid
    #215930
    Purpose
    Fragmid fragment: (guide cassette) 2xPP7 & 2xMS2 in trRNA
    Depositor
    John Doench
    Article
    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi
    Has Service
    Cloning Grade DNA
    Insert
    U6_v1; sgCD4_v3 [Sp]; trRNA_v14 [Sp]; CS2
    Use
    CRISPR; Fragment
    Tags
    Expression
    Mutation
    Promoter
    Available since
    April 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    215930-plasmid-map-sequence-id-431446

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  40. AA017

    Plasmid
    #215928
    Purpose
    Fragmid fragment: (guide cassette) 2xPP7 & 2xMS2 in trRNA
    Depositor
    John Doench
    Article
    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi
    Has Service
    Cloning Grade DNA
    Insert
    U6_v1; BsmBI_v0; BsmBI_v1; trRNA_v14 [Sp]; CS2
    Use
    CRISPR; Fragment
    Tags
    Expression
    Mutation
    Promoter
    Available since
    April 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    215928-plasmid-map-sequence-id-431437

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  48. AA233

    Plasmid
    #215941
    Purpose
    Fragmid fragment: (guide cassette) guide expression; positive control cell surface with 2xPP7 & 2xMS2 in trRNA
    Depositor
    John Doench
    Article
    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi
    Has Service
    Cloning Grade DNA
    Insert
    U6_v1; sgCD274_v1 [Sp]; trRNA_v14 [Sp]
    Use
    CRISPR; Fragment
    Tags
    Expression
    Mutation
    Promoter
    Available since
    April 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    215941-plasmid-map-sequence-id-431532

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  49. AA232

    Plasmid
    #215940
    Purpose
    Fragmid fragment: (guide cassette) guide expression; positive control cell surface with 2xPP7 & 2xMS2 in trRNA
    Depositor
    John Doench
    Article
    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi
    Has Service
    Cloning Grade DNA
    Insert
    U6_v1; sgCD47_v4 [Sp]; trRNA_v14 [Sp]
    Use
    CRISPR; Fragment
    Tags
    Expression
    Mutation
    Promoter
    Available since
    April 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    215940-plasmid-map-sequence-id-431480

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Showing: 151 - 200 of 218 results