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Showing: 151 - 200 of 18313 results

  20. PX852

    Plasmid
    #62884
    Purpose
    C-term SpCas9 piece of inducible split-4 (Cas9(C)-FKBP split-4).
    Depositor
    Feng Zhang
    Article
    Zetsche et al Nat Biotechnol. 2015 Feb 2. doi: 10.1038
    Insert
    SpCas9 (aa536-1368)
    Use
    CRISPR
    Tags
    FKBP12, NLS SV40, and NLS nucleoplasmin
    Expression
    Mammalian
    Mutation
    Promoter
    CBh
    Available since
    March 4, 2015
    Availability
    Academic Institutions and Nonprofits only
    62884-plasmid-map-sequence-id-105906

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  21. pBAD-6xHis-AviTag-ALFAtag-eGFP

    Plasmid
    #195854
    Purpose
    Bacterial expression vector encoding 6xHis-AviTag-ALFAtag-eGFP. Co-expression in cells with biotin ligase (BirA) results in efficient biotinylation of the AviTag sequence.
    Depositor
    John Ngo
    Article
    Sloas et al Nat Biotechnol. 2023 Jan 16. doi: 10.103
    Insert
    6xHis-AviTag-ALFAtag-eGFP
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 1, 2023
    Availability
    Academic Institutions and Nonprofits only
    195854-plasmid-map-sequence-id-383965

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  22. PX856

    Plasmid
    #62888
    Purpose
    C-term SpCas9 piece of inducible transcriptional activator (dCas9(C)-FKBP-2xNLS-VP64)
    Depositor
    Feng Zhang
    Article
    Zetsche et al Nat Biotechnol. 2015 Feb 2. doi: 10.1038
    Insert
    SpCas9 (aa536-1368)
    Use
    CRISPR
    Tags
    FKBP12, NLS, NLS SV40, and VP64
    Expression
    Mammalian
    Mutation
    Asparagine 863 to Alanine (N863A)
    Promoter
    CBh
    Available since
    March 16, 2015
    Availability
    Academic Institutions and Nonprofits only
    62888-plasmid-map-sequence-id-105911

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  23. PX855

    Plasmid
    #62887
    Purpose
    N-term SpCas9 piece of inducible transcriptional activator (dCas9(N)-FRB-2xNES)
    Depositor
    Feng Zhang
    Article
    Zetsche et al Nat Biotechnol. 2015 Feb 2. doi: 10.1038
    Insert
    SpCas9 (aa 2-535)
    Use
    CRISPR
    Tags
    FRB and NES PTK2
    Expression
    Mammalian
    Mutation
    Aspartic acide 10 to Alanine (D10A)
    Promoter
    CBh
    Available since
    March 5, 2015
    Availability
    Academic Institutions and Nonprofits only
    62887-plasmid-map-sequence-id-105910

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  26. PX854

    Plasmid
    #62886
    Purpose
    C-term SpCas9 piece of inducible split-5 (Cas9(C)-FKBP split-5).
    Depositor
    Feng Zhang
    Article
    Zetsche et al Nat Biotechnol. 2015 Feb 2. doi: 10.1038
    Insert
    SpCas9 (aa573-1368)
    Use
    CRISPR
    Tags
    FKBP12, NLS SV40, and NLS nucleoplasmin
    Expression
    Mammalian
    Mutation
    Promoter
    CBh
    Available since
    March 16, 2015
    Availability
    Academic Institutions and Nonprofits only
    62886-plasmid-map-sequence-id-105909

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  41. LV TRE-GFP-TMD-DLL1

    Plasmid
    #194936
    Purpose
    LV construct encoding GFP-TMD-DLL1 ligand under dox-inducible TRE promoter and containing a PGK-rtTA-M2-IRES-Puro regulator/resistance marker cassette.
    Depositor
    John Ngo
    Article
    Sloas et al Nat Biotechnol. 2023 Jan 16. doi: 10.103
    Insert
    eGFP-(PDGFR)TMD-rDLL1 ICD (WT)
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    194936-plasmid-map-sequence-id-386565

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Showing: 151 - 200 of 18313 results