We narrowed to 2,857 results for: BFP

1. pM-mTagBFP2

   Plasmid

   #228615

   Purpose

   Mammalian expression of mTagBFP2 and extra insert, for MuSIC barcode construction

   Depositor

   Marc Birtwistle

   Article

   Lu et al ACS Synth Biol. 2025 May 16;14(5):1533-1

   Insert

   mTagBFP2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   We introduced some silent mutations to avoid esse…

   Promoter

   CMV

   Available since

   Jan. 9, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pRRlsinPGK_BFP2_WPRE

   Plasmid

   #91787

   Purpose

   Constitutive expression of mTagBFP2 using PGK promoter in a lentiviral backbone.

   Depositor

   Beverly Torok-Storb

   Insert

   mTagBFP2
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   PGK

   Available since

   June 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pRDA174_EF1a_EnAsCas12a_BFP

   Plasmid

   #196724

   Purpose

   Sortable and selectable EnAsCas12a.

   Depositor

   Bernhard Schmierer

   Insert

   enASCas12a-HA-T2A-BSD-TagBFP
   

   Use

   CRISPR and Lentiviral

   Tags

   Nucleoplasmin NLS - HA and SV40 NLS

   Expression

   Mutation

   E174R, S542R, K548R

   Promoter

   EF1a

   Available since

   April 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLenti6.2_TagBFP

   Plasmid

   #113724

   Purpose

   Expresses the fluorescent protein TagBFP in a lentiviral backbone

   Depositor

   Vanessa LaPointe

   Insert

   TagBFP
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCVL.SFFV.Kozak.HA.NLS.SceOpt.IRES.BFP

   Plasmid

   #45574

   Purpose

   Expresses I-Sce I nuclease in mammalian cells with BFP tracker in lentiviral backbone

   Depositor

   Andrew Scharenberg

   Article

   Kuhar et al Nucleic Acids Res. 2013 Oct 10.

   Insert

   co I-Sce I
   

   Use

   Lentiviral

   Tags

   HA, IRES BFP, and NLS

   Expression

   Mammalian

   Mutation

   co indicates codon optimization for mammalian exp…

   Promoter

   SFFV

   Available since

   Nov. 4, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCDNA3_LuxSit_i_mTagBFP2

   Plasmid

   #194300

   Purpose

   Mammalian expression of the de novo luciferase, LuxSit-i

   Depositor

   Andy Yeh, David Baker

   Article

   Yeh et al Nature. 2023 Feb;614(7949):774-780. doi:

   Insert

   LuxSit-i
   

   Use

   Tags

   mTagBFP2

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. sgRNA.SFFV.tBFP

   Plasmid

   #169940

   Purpose

   Expresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and tBFP from SFFV promoter as the marker for infection. 3rd generation lentiviral backbone.

   Depositor

   Benjamin Ebert

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. TagBFP(S2)2(S4)2-gs

   Plasmid

   #228072

   Purpose

   Mammalian expression of coiled coil segments (S2)x2-(S4)x2, with short flexible linkers and N-terminal TagBFP; for phase separation and forming of liquid condensates in mammalian cells.

   Depositor

   Roman Jerala

   Article

   Ramsak et al Nat Commun. 2023 Dec 2;14(1):7973. doi:

   Insert

   BFP, S2, S4
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. EBFP2-ER-5

   Plasmid

   #55240

   Purpose

   Localization: Endoplasmic Reticulum, Excitation: 383, Emission: 448

   Depositor

   Michael Davidson

   Insert

   ER (CALR Human)
   

   Use

   Tags

   EBFP2

   Expression

   Mammalian

   Mutation

   ER Signal Peptide

   Promoter

   CMV

   Available since

   Oct. 22, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

10. pMax_BFP_A191G

    Plasmid

    #177823

    Purpose

    Site-specific mutagenesis of tagBFP for the purposes of creating a site-specific U:A mispair for the purposes of measuring UDG activity via Host Cell Reactivation (HCR).

    Depositor

    Leona Samson, Zachary Nagel

    Article

    Nagel et al Proc Natl Acad Sci U S A. 2014 May 6;111

    Insert

    tagBFP_A191G
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    A191G

    Promoter

    CMV

    Available since

    Dec. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. ERoxBFP

    Plasmid

    #68126

    Purpose

    mammalian expression of ER localized oxBFP

    Depositor

    Erik Snapp

    Article

    Costantini et al Nat Commun. 2015 Jul 9;6:7670. doi: 10.1

    Insert

    oxBFP
    

    Use

    Tags

    KDEL ER retention sequence and bovine prolactin s…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 10, 2015

    Availability

    Academic Institutions and Nonprofits only

12. MSCV_JAK3_IRES_BFP

    Plasmid

    #206291

    Purpose

    Expresses the wild type form of human JAK3.

    Depositor

    Charles de Bock

    Article

    Omari et al Heliyon. 2023 Nov 8;9(11):e22085. doi: 1

    Insert

    JAK3 (JAK3 Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pEX.sEF1a.Kozak.HA.NLS.Sce.T2A.BFP

    Plasmid

    #45565

    Purpose

    Expresses I-Sce I nuclease in mammalian cells and BFP as a tracker

    Depositor

    Andrew Scharenberg

    Article

    Kuhar et al Nucleic Acids Res. 2013 Oct 10.

    Inserts

    I-Sce I Nuclease
    BFP
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 4, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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14. pEX.d14GFP.sEF1a.Kozak.HA.NLS.Sce.T2A.BFP

    Plasmid

    #45564

    Purpose

    Expresses I-Sce I nuclease and BFP tracker in mammalian cells, codes for eGFP donor sequence

    Depositor

    Andrew Scharenberg

    Article

    Kuhar et al Nucleic Acids Res. 2013 Oct 10.

    Inserts

    d14 eGFP donor
    I-Sce I Nuclease
    BFP
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    deleted n' 15 amino acids

    Promoter

    Available since

    Nov. 4, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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15. pExp_CAG:EBFP2

    Plasmid

    #51790

    Purpose

    Constitutively expresses blue fluorescent protein, EBFP2

    Depositor

    Ron Weiss

    Article

    Davidsohn et al ACS Synth Biol. 2014 Nov 17.

    Insert

    EBFP2
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    March 4, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCVL.SFFV.Kozak.HA.NLS.Y2AniK227M.IRES.BFP

    Plasmid

    #45579

    Purpose

    Expresses K227M nickase variant of I-Ani I nuclease in mammalian cells with BFP tracker

    Depositor

    Andrew Scharenberg

    Article

    Kuhar et al Nucleic Acids Res. 2013 Oct 10.

    Insert

    coY2 Ani K227M
    

    Use

    Lentiviral

    Tags

    HA, IRES BFP, and NLS

    Expression

    Mammalian

    Mutation

    co indicates codon optimization for mammalian exp…

    Promoter

    SFFV

    Available since

    Nov. 4, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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17. pSA_2_mTagBFP2_synCoTC

    Plasmid

    #199474

    Purpose

    Site directed zebrafish mutagenesis. sgRNA GAAGATCGGCCACTACATTC

    Depositor

    Robert Bryson-Richardson

    Article

    Miles et al bioRxiv 2023

    Insert

    mTagBFP2
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pSA_0_mTagBFP2_synCoTC

    Plasmid

    #199472

    Purpose

    Site directed zebrafish mutagenesis. sgRNA GAAGATCGGCCACTACATTC

    Depositor

    Robert Bryson-Richardson

    Article

    Miles et al bioRxiv 2023

    Insert

    mTagBFP2
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pHR_Gal4UAS_FliC_IRES_mCherry_PGK_tBFP

    Plasmid

    #85438

    Purpose

    Lentiviral vector for Gal4 inducible S. Typhi Flagellin with mCherry reporter and constitutive tBFP expression

    Depositor

    Wendell Lim

    Article

    Roybal et al Cell. 2016 Oct 6;167(2):419-432.e16. doi

    Insert

    S. typhi FliC
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 23, 2017

    Availability

    Academic Institutions and Nonprofits only

20. XClone_Cas9_KRAB_T2A_BFP

    Plasmid

    #141208

    Purpose

    Tunable and temporal expression control of dCas9

    Depositor

    Rory Johnson

    Insert

    dCas9
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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