We narrowed to 2,021 results for: SP6

1. pSP6-EBNA(2A+DBD)

   Plasmid

   #98749

   Purpose

   In vitro transcription of modified EBNA1 mRNA

   Depositor

   Sara Howden, Jim Vadolas

   Article

   Howden et al Hum Gene Ther. 2006 Aug;17(8):833-44.

   Insert

   Modified EBNA1
   

   Use

   In vitro transcription

   Tags

   Expression

   Mutation

   Promoter

   SP6

   Available since

   Aug. 2, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET23b-Casp6-His

   Plasmid

   #11823

   Depositor

   Guy Salvesen

   Article

   Zhou et al J Biol Chem. 1997 Mar 21. 272(12):7797-8

   Insert

   Caspase 6 (CASP6 Human)
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   July 19, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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3. Hsp68-LacZ-Gibson

   Plasmid

   #170102

   Purpose

   Transgenic LacZ reporter construct for validation of enhancer sequences (inserted via Gibson-cloning). Can be introduced into the mouse genome via random integration following pro-nuclear injection.

   Depositor

   Len Pennacchio

   Type

   Empty backbone

   Use

   Mouse Targeting

   Tags

   Expression

   Mutation

   Promoter

   Mouse Hsp68 minimal promoter

   Available since

   Oct. 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. Hsp68-CreER(TM) (DM#264)

   Plasmid

   #15028

   Depositor

   Douglas Melton

   Insert

   Cre-ER(TM)
   

   Use

   Cre/Lox; For making transgenic constructs

   Tags

   Hsp68 promoter and polyA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 18, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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5. pEntry_HCoV-OC43-nsp6

   Plasmid

   #168934

   Purpose

   Gateway-compatible Entry vector

   Depositor

   Pascal Falter-Braun

   Article

   Weller et al G3 (Bethesda). 2023 Jul 5;13(7):jkad105.

   Insert

   HCoV-OC43-nsp6 (ORF1ab )
   

   Use

   Gateway-compatible entry vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSP64-hERG1a T623A

   Plasmid

   #53055

   Purpose

   Expresses alpha subunit of T623A hERG1a K channel in Xenopus oocytes

   Depositor

   Michael Sanguinetti

   Article

   Mitcheson et al Proc Natl Acad Sci U S A. 2000 Oct 24;97

   Insert

   Potassium voltage-gated channel subfamily H member 2 (KCNH2 Human)
   

   Use

   Xenopus oocyte expression

   Tags

   Expression

   Mutation

   changed Threonine 623 to Alanine

   Promoter

   SP6

   Available since

   June 9, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. pSP64-hERG1a S624A

   Plasmid

   #53056

   Purpose

   Expresses alpha subunit of S624A hERG1a K channel in Xenopus oocytes

   Depositor

   Michael Sanguinetti

   Article

   Mitcheson et al Proc Natl Acad Sci U S A. 2000 Oct 24;97

   Insert

   Potassium voltage-gated channel subfamily H member 2 (KCNH2 Human)
   

   Use

   Xenopus oocyte expression

   Tags

   Expression

   Mutation

   changed Serine 624 to Alanine

   Promoter

   SP6

   Available since

   June 9, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. SP6-sgRNA-scaffold

   Plasmid

   #47912

   Purpose

   Scaffold into which clone a 20 bp targeting sequence to generate a plasmid for in vitro transcription of an sgRNA using SP6 RNA polymerase for use in CRISPR-Cas by RNA injection

   Depositor

   Paul Sternberg

   Article

   Chiu et al Genetics. 2013 Aug 26.

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   sgRNA 3' end

   Expression

   Worm

   Mutation

   Promoter

   SP6

   Available since

   Sept. 13, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSP64-Lifeact-GFP

   Plasmid

   #135601

   Purpose

   Expression of Lifeact-GFP mRNA for microinjection for visualization of actin in S. purpuratus

   Depositor

   Mamiko Yajima

   Article

   Uchida et al Dev Biol. 2018 Sep 1;441(1):19-30. doi:

   Insert

   Lifeact-GFP
   

   Use

   In vitro transcription (mrna synthesis)

   Tags

   EGFP

   Expression

   Mutation

   Promoter

   Unknown

   Available since

   Jan. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pISceI Dusp6:memGFP

    Plasmid

    #32668

    Purpose

    Expresses membrane localized EGFP under the Dusp6 promoter

    Depositor

    Michael Tsang

    Article

    Wang et al Development. 2011 Jan;138(1):45-54. Epub

    Insert

    Dusp6 promoter (dusp6 Zebrafish)
    

    Use

    Zebrafish transgenesis

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 28, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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11. SP6-hCas9-Ce-mRNA

    Plasmid

    #47911

    Purpose

    In vitro transcription of a mRNA encoding humanized Cas9 nuclease, with 3' UTRs suitable for germline expression in C. elegans, for doing CRISPR-Cas by RNA injection. Uses SP6 RNA polymerase.

    Depositor

    Paul Sternberg

    Article

    Chiu et al Genetics. 2013 Aug 26.

    Insert

    hCas9 with C. elegans 5' and 3' UTRs
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    SP6

    Available since

    Sept. 13, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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12. pEntry_SARS-CoV-2-nsp6

    Plasmid

    #168869

    Purpose

    Gateway-compatible Entry vector

    Depositor

    Pascal Falter-Braun

    Article

    Weller et al G3 (Bethesda). 2023 Jul 5;13(7):jkad105.

    Insert

    SARS-CoV-2-nsp6 (ORF1ab )
    

    Use

    Gateway-compatible entry vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pISceI Dusp6:D2EGFP

    Plasmid

    #32667

    Depositor

    Michael Tsang

    Article

    Molina et al BMC Dev Biol. 2007 Jun 6;7:62.

    Insert

    Dusp6 promoter (dusp6 Zebrafish)
    

    Use

    Zebrafish transgenesis

    Tags

    EGFP

    Expression

    Mutation

    Promoter

    Available since

    Nov. 28, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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14. pEntry_HCoV-NL63-nsp6

    Plasmid

    #168915

    Purpose

    Gateway-compatible Entry vector

    Depositor

    Pascal Falter-Braun

    Article

    Weller et al G3 (Bethesda). 2023 Jul 5;13(7):jkad105.

    Insert

    HCoV-NL63-nsp6 (ORF1ab )
    

    Use

    Gateway-compatible entry vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pSP64-Vasa-GFP

    Plasmid

    #135595

    Purpose

    Expression of Vasa-GFP mRNA for microinjection for visualization of Vasa protein dynamics in S. purpuratus

    Depositor

    Mamiko Yajima

    Article

    Yajima et al Development. 2015 Jun 1;142(11):1960-70.

    Insert

    Vasa-GFP
    

    Use

    In vitro transcription (mrna synthesis)

    Tags

    EGFP

    Expression

    Mutation

    Promoter

    SP6

    Available since

    Jan. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. R777-E043 Hs.DUSP6

    Plasmid

    #70327

    Purpose

    Gateway ORF clone of human DUSP6 [NM_001946.3] with stop codon (for native or N-terminal fusions)

    Depositor

    Dominic Esposito

    Insert

    DUSP6 (DUSP6 Human)
    

    Use

    Gateway entry clone

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pSP64T tBMPR (DM#135)

    Plasmid

    #15067

    Depositor

    Douglas Melton

    Article

    Graff et al Cell. 1994 Oct 7. 79(1):169-79.

    Insert

    BMP receptor (bmpr1a Frog)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Truncated dominant negative form

    Promoter

    Available since

    Oct. 18, 2007

    Availability

    Academic Institutions and Nonprofits only

18. p3E-Dusp6DM_S159/197A

    Plasmid

    #67684

    Purpose

    3' entry clone with activated Dusp6 (S159/167A)

    Depositor

    Nathan Lawson

    Article

    Shin et al Development. 2016 Oct 15;143(20):3796-38

    Insert

    dusp6 (dusp6 Zebrafish)
    

    Use

    Tags

    Expression

    Mutation

    S159A, S197A

    Promoter

    Available since

    Aug. 7, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. pSP64-membrane-mCherry

    Plasmid

    #135598

    Purpose

    Expression of membrane-mCherry mRNA for microinjection for visualization of membrane in S. pupuratus

    Depositor

    Mamiko Yajima

    Article

    Uchida et al Dev Biol. 2018 Sep 1;441(1):19-30. doi:

    Insert

    membrane-mCherry
    

    Use

    In vitro transcription (mrna synthesis)

    Tags

    mCherry

    Expression

    Mutation

    Promoter

    Unknown

    Available since

    Jan. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pSP64T-ythdf2-3xflag

    Plasmid

    #164489

    Purpose

    Expresses zebrafish ythdf2 with 3x flag tag, for mRNA injection

    Depositor

    Antonio Giraldez

    Article

    Kontur et al Cell Rep. 2020 Dec 29;33(13):108598. doi

    Insert

    ythdf2 (ythdf2 Zebrafish)
    

    Use

    Production of mrna for zebrafish injection.

    Tags

    3x-flagtag

    Expression

    Mutation

    Promoter

    SP6

    Available since

    April 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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