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We narrowed to 4,931 results for: codon optimized

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Showing: 61 - 80 of 4931 results

  1. MSP1830

    Plasmid
    #70708
    Purpose
    Human expression plasmid for SaCas9 KKH variant: CAG-humanSaCas9(E782K/N968K/R1015H)-NLS-3xFLAG
    Depositor
    Keith Joung
    Publication
    Kleinstiver et al Nat Biotechnol. 2015 Nov 2. doi: 10.1038
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    mammalian codon-optimized KKH variant Staphylococcus aureus Cas9
    Use
    CRISPR
    Tags
    NLS-3xFLAG
    Expression
    Mammalian
    Mutation
    E782K, N968K and R1015H in SaCas9
    Promoter
    CAG
    Available since
    Nov. 6, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    5 Articles
    70708-plasmid-map-sequence-id-364684

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  2. eeBxb1_IVT

    Plasmid
    #222342
    Purpose
    Plasmid for evolved and engineered Bxb1 mRNA in vitro transcription template
    Depositor
    David Liu
    Publication
    Pandey et al Nat Biomed Eng. 2024 Jun 10. doi: 10.103
    Insert
    Codon-optimized evolved and engineered Bxb1
    Use
    Template for in vitro transcription
    Promoter
    T7 (inactivated)
    Available since
    July 25, 2024
    Availability
    Academic Institutions and Nonprofits only
    222342-plasmid-map-sequence-id-440249

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  3. MSP469

    Plasmid
    #65771
    Purpose
    Human expression vector for SpCas9 VQR variant: CMV-T7-humanSpCas9(D1135V/R1335Q/T1337R)-NLS-3xFLAG (VQR variant)
    Depositor
    Keith Joung
    Publication
    Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    mammalian codon-optimized Streptococcus pyogenes Cas9 (D1135V/R1335Q/T1337R)-NLS-3XFlag
    Use
    CRISPR
    Tags
    3x FLAG and NLS
    Expression
    Mammalian
    Mutation
    D1135V, R1335Q, and T1337R mutations in Cas9
    Promoter
    CMV & T7
    Available since
    June 22, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    6 Articles
    65771-plasmid-map-sequence-id-112653

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  4. pVE13300

    Plasmid
    #137715
    Purpose
    Expresses impLbCas12a in mammalian cells.
    Depositor
    Ervin Welker
    Publication
    Toth et al Nucleic Acids Res. 2020 Feb 28. pii: 576
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    impLbCas12a
    Tags
    3xHA, NLS, and SV40 NLS
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    March 16, 2020
    Availability
    Academic Institutions and Nonprofits only
    137715-plasmid-map-sequence-id-274023

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  5. pCRISPRyl

    Plasmid
    #70007
    Purpose
    CRISPR/Cas9 vector for Yarrowia lipolytica, with AvrII site for sgRNA insertion
    Depositor
    Ian Wheeldon
    Publication
    Schwartz et al ACS Synth Biol. 2015 Dec 29.
    Inserts
    Codon optimized Cas9 from S. pyogenes
    sgRNA expression cassette
    Use
    CRISPR and Synthetic Biology
    Expression
    Yeast
    Promoter
    SCR1'-tRNA and UAS1B8-TEF(136)
    Available since
    Feb. 16, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    16 Articles
    70007-plasmid-map-sequence-id-516817

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  7. pZyxin-mMaple3

    Plasmid
    #101151
    Purpose
    Fluorescent protein for superresolution imaging and a focal adhesion protein for binding substrate
    Depositor
    Xiaowei Zhuang
    Publication
    Wang et al Proc Natl Acad Sci U S A. 2014 Jun 10;11
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    human codon optimized mMaple3 fused to zyxin
    Expression
    Mammalian
    Promoter
    cmv
    Available since
    March 19, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    101151-plasmid-map-sequence-id-205969

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  9. pCAG-enAsCas12a-HF1(E174R/N282A/S542R/K548R)-NLS(nuc)-3xHA (AAS1815)

    Plasmid
    #107942
    Purpose
    Mammalian expression plasmid for human codon optimized enAsCas12a-HF1 (enhanced high-fidelity AsCas12a) encoding E174R/N282A/S542R/K548R substitutions
    Depositor
    Keith Joung, Benjamin Kleinstiver
    Publication
    Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    human codon optimized enAsCas12a-HF1 (E174R/N282A/S542R/K548R)
    Tags
    3x HA and NLS (nucleoplasmin)
    Expression
    Mammalian
    Mutation
    E174R, S542R, K548R, and N282A
    Promoter
    CAG
    Available since
    Feb. 11, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    4 Articles
    107942-plasmid-map-sequence-id-259760

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  10. pEF1a-hMLH1NTD-NLS

    Plasmid
    #174826
    Purpose
    Mammalian expression of human MLH1NTD-NLS (codon optimized)
    Depositor
    David Liu
    Publication
    Chen et al Cell. 2021 Oct 28;184(22):5635-5652.e29.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    human MLH1_NTD-NLS (codon optimized)
    Tags
    SV40 bpNLS
    Expression
    Mammalian
    Mutation
    truncation of residues 1-335
    Promoter
    EF1a
    Available since
    Oct. 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    174826-plasmid-map-sequence-id-343633

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  15. VP12

    Plasmid
    #72247
    Purpose
    Human expression plasmid for SpCas9-HF1 variant: CMV-T7-humanSpCas9-HF1(N497A, R661A, Q695A, Q926A)-NLS-3xFLAG
    Depositor
    Keith Joung
    Publication
    Kleinstiver et al Nature. 2016 Jan 6. doi: 10.1038/nature1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    mammalian codon-optimized Streptococcus pyogenes Cas9 HF1(N497A/R661A/Q695A/Q926A)-NLS-3xFlag
    Use
    CRISPR
    Tags
    3x FLAG and NLS
    Expression
    Mammalian
    Mutation
    N497A, R661A, Q695A and Q926A
    Promoter
    CMV
    Available since
    Jan. 6, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    29 Articles
    72247-plasmid-map-sequence-id-485302

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  16. pEJS654 All-in-One AAV-sgRNA-hNmeCas9

    Plasmid
    #112139
    Purpose
    Delivery of human-codon-optimized Cas9 from Neisseria meningitidis (NmeCas9) and its single-guide RNA in a single AAV vector for in vivo genome editing.
    Depositor
    Erik Sontheimer
    Publication
    Ibraheim et al Genome Biol. 2018 Sep 19;19(1):137. doi:
    Inserts
    sgRNA scaffold
    Human-codon-optimized NmeCas9
    Use
    AAV, CRISPR, and Mouse Targeting
    Expression
    Mammalian
    Promoter
    U1a and U6
    Available since
    Aug. 13, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    112139-plasmid-map-sequence-id-217823

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  17. BPK764

    Plasmid
    #65767
    Purpose
    Bacterial expression plasmid for SpCas9 & sgRNA (need to clone spacer into BsaI sites): T7-humanSpCas9-NLS-3xFLAG-T7-BsaIcassette-Sp-sgRNA
    Depositor
    Keith Joung
    Publication
    Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature
    Insert
    mammalian codon-optimized Streptococcus pyogenes Cas9-NLS-3XFlag, and SpCas9 gRNA
    Use
    CRISPR
    Tags
    3x FLAG and NLS
    Expression
    Bacterial
    Promoter
    T7 (x2)
    Available since
    June 24, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    65767-plasmid-map-sequence-id-471631

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  18. pCAG-T3-hCAS-pA

    Plasmid
    #48625
    Purpose
    Expresses CAS9 nuclease in mammalian cells. Used to modify genome of mouse embroys
    Depositor
    Wataru Fujii, Kunihiko Naito
    Publication
    Fujii et al Nucleic Acids Res. 2013 Aug 30.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Codon optimized Cas9
    Use
    CRISPR
    Tags
    FLAG, NLS, and polyA
    Expression
    Mammalian
    Promoter
    CAG promoter
    Available since
    Nov. 4, 2013
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    20 Articles
    48625-plasmid-map-sequence-id-116741

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  19. MSP1101

    Plasmid
    #65773
    Purpose
    Human expression vector for SpCas9 VRER variant: CMV-T7-humanSpCas9(D1135V/G1218R/R1335E/T1337R)-NLS-3xFLAG (VRER variant)
    Depositor
    Keith Joung
    Publication
    Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    mammalian codon-optimized Streptococcus pyogenes Cas9 (D1135V/G1218R/R1335E/T1337R)-NLS-3XFlag
    Use
    CRISPR
    Tags
    3x FLAG and NLS
    Expression
    Mammalian
    Mutation
    D1135V, G1218R, R1335E, and T1337R mutations in C…
    Promoter
    CMV & T7
    Available since
    June 22, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    65773-plasmid-map-sequence-id-112657

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Showing: 61 - 80 of 4931 results