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Plasmid#180361Purposemammalian co-expression of human SEPT9_i3 fused to GFP10 and of human SEPT7 Gmut2 fused to GFP11DepositorUseLentiviralTagsGFP10 and GFP11ExpressionMammalianMutationSEPT7 H279DPromoterbidirectional TREAvailable sinceDec. 1, 2022AvailabilityAcademic Institutions and Nonprofits only
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pnEA-vH_His-TEV-SEPT2 NCmut-msfGFP
Plasmid#180311Purposebacterial expression of human SEPT2 NCmut fused to monomeric superfolder GFPDepositorInsertSEPTIN2 (SEPTIN2 Human)
UseTagsHis6-TEV and msfGFPExpressionBacterialMutationSEPT2 F20D, V27DPromoterAvailable sinceDec. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pnEA-vH_His-TEV-SEPT2 NCmut-msfGFP_SEPT6
Plasmid#180312Purposebacterial co-expression of human SEPT2 NCmut fused to monomeric superfolder GFP and of human SEPT6DepositorUseTagsHis6-TEV and msfGFPExpressionBacterialMutationSEPT2 F20D, V27DPromoterAvailable sinceDec. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
K-to-R mutant NPRL3(1-569) in pLJM60, codon-optimized
Plasmid#184563PurposeCMV-driven expression of an untagged mutant NPRL3 (core subunit of GATOR1) — codon-optimized sequence for stable expression in mammalian cells. All lysine residues were mutated to arginines.DepositorInsertC16orf35, CGTHBA, FFEVF3, HS-40, MARE, NPR3, RMD11 (NPRL3 Human)
UseLentiviralTagsExpressionMammalianMutationAll lysines were mutated to arginines. Codon-opti…PromoterCMVAvailable sinceNov. 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
psiCHECK2-mMOV10-3'UTR-MRE16+MRE153-mut
Plasmid#178910Purposedual luminescence reporter to assess miRNA responsiveness in the 3'UTR of mouse Mov10 RNA helicaseDepositorInsert3'UTR mMov10 (Mov10 Mouse)
UseLuciferaseTagsExpressionMutationCTG>AGT and TGC>CAT mir16+153 mutatedPromoterSV40 promoterAvailable sinceNov. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
EK0441 CMV-TO-BFPmut-stop-EGFP.t2 (FLP-IN)
Plasmid#191161PurposeExpression of a trigger sequence from EGFP CDS in 3' UTR context in mammalian cellsDepositorInsertEGFP.t2
UseTagsExpressionMammalianMutationWTPromoterCMV-TOAvailable sinceOct. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pmiRGLO Dual-Luciferase-PDAP1-3'UTR-Mut4x
Plasmid#182256PurposeHuman PDAP1 3` UTR region mutated in 4x binding site for miR-150 cloned downstream of luciferase reporter geneDepositorInsert3' UTR region of PDAP1 gene mutated in 4 binding sites for mir-150
UseLuciferaseTagsExpressionMammalianMutationPromoterAvailable sinceApril 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pmiRGLO Dual-Luciferase-PDAP1-3'UTR-Mut2x
Plasmid#182254PurposeHuman PDAP1 3` UTR region mutated in 2x binding site for miR-150 cloned downstream of luciferase reporter geneDepositorInsert3' UTR region of PDAP1 gene mutated in 2 binding sites for mir-150
UseLuciferaseTagsExpressionMammalianMutationPromoterAvailable sinceApril 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pmiRGLO Dual-Luciferase-PDAP1-3'UTR-Mut1x
Plasmid#182253PurposeHuman PDAP1 3` UTR region mutated in 1x binding site for miR-150 cloned downstream of luciferase reporter geneDepositorInsert3' UTR region of PDAP1 gene mutated in 1 binding site for mir-150
UseLuciferaseTagsExpressionMammalianMutationPromoterAvailable sinceApril 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pmiRGLO Dual-Luciferase-PDAP1-3'UTR-Mut3x
Plasmid#182255PurposeHuman PDAP1 3` UTR region mutated in 3x binding site for miR-150 cloned downstream of luciferase reporter geneDepositorInsert3' UTR region of PDAP1 gene mutated in 3 binding sites for mir-150
UseLuciferaseTagsExpressionMammalianMutationPromoterAvailable sinceApril 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLV-Hygro-Ef1A-Myc-POLB(PAMmut)
Plasmid#176150PurposeN-terminus Myc-tagged POLB containing a mutation in the PAM site used by POLB gRNA1 & a hygromycin resistance cassetteDepositorInsertPolB (POLB Human)
UseLentiviralTagsMYCExpressionMammalianMutationMutation in the PAM site used by POLB gRNA1PromoterEF1AAvailable sinceFeb. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pmirGlo-RBM8A-3'UTRAluY-Sz mut 2598-4066
Plasmid#171135PurposereporterDepositorInsertRBM8A 3'UTR (RBM8A Human)
UseTagsExpressionMammalianMutationA3302G, A3356G, A3373G, A3389G,A3451G, T3729C, A3…PromoterAvailable sinceDec. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMXS-IRES-Blast 3XFLAG-CHP1-myr_mut
Plasmid#173167PurposeRetroviral vector to express sgRNA resistant 3XFLAG tagged human CHP1 with myristoylation site mutationDepositorInsertCalcineurin Like EF-Hand Protein 1 (CHP1 Human)
UseRetroviralTags3XFLAGExpressionMutationSynonymous mutations at sgRNA sites, G2A, S6APromoterMMLVAvailable sinceAug. 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
p5E-161K enhancer-ETS-CORE-MUT
Plasmid#138372PurposeGateway compatible 5' entry clone contains human modified 161k enhancer sequence. with ETS binding sites mutatedDepositorInserthuman 161K human enhancer with ETS bind sites mutated
UseGateway compatibleTagsExpressionMutationETS factor binding sites mutatedPromoterNONEAvailable sinceFeb. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
dre-cdk2 3'UTR sicheck2 199 mut
Plasmid#121122Purposeluciferase assayDepositorInsertdre-cdk2 3'UTR mutated 199 binding (cdk2 Zebrafish)
UseTagsExpressionBacterialMutationmiR-199 seed binding site mutatedPromoterAvailable sinceSept. 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
Fibronectin-human-mutated N-terminal (N) O-Glycosylation site
Plasmid#120404Purposeenables eukaryotic expression of human plasma fibronectin in which the first O-glycosylation site was mutated from threonine to serineDepositorInsertFibronectin (FN1 Human)
UseTagsExpressionMammalianMutationContaining complete variable region with a mutati…PromoterCMVAvailable sinceJan. 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
pPICZ-pOst1-pro-alphaf(MUT2)-E2-Crimson
Plasmid#117663PurposeTest intracellular localisation of E2-Crimson and study the secretion efficiencyDepositorInsertpOst1-pro-af(MUT2)-E2-Crimson
UseTagsExpressionYeastMutationThis plasmid encodes the fluorescent protein E2-C…PromoterpAOX1Available sinceDec. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLex-FLAG-FASTKD4 mutant delta 226
Plasmid#73621PurposeExpression vector containing FASTKD4 mutant delta 226DepositorInsertFASTKD4 (TBRG4 Human)
UseTagsExpressionMutationY616A, L617A, K618A, K620APromoterAvailable sinceOct. 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
1287 p3E-mut let-7 etv2 3’ UTR
Plasmid#49011PurposeZebrafish Long length etv2 3' UTR with five let7 binding site mutations in gateway 3' Entry vectorDepositorInsertets variant gene 2 (etsrp Zebrafish)
UseGateway 3' entryTagsExpressionMutationMutated the Let7 miRNA binding sitesPromoterAvailable sinceNov. 7, 2013AvailabilityAcademic Institutions and Nonprofits only -
pmyc-GFP-TNRC6A-del.GW-II-NES-mut
Plasmid#42038DepositorInsertTNRC6A (TNRC6A Human)
UseTagsEGFP and MycExpressionMammalianMutationdeleted aa716-772, F951A, F955A, I958A, F960APromoterCMVAvailable sinceMarch 7, 2013AvailabilityAcademic Institutions and Nonprofits only -
pmyc-GFP-TNRC6A-del.GW-I-NES-mut
Plasmid#42037DepositorInsertTNRC6A (TNRC6A Human)
UseTagsEGFP and MycExpressionMammalianMutationdeleted aa457-532, F951A, F955A, I958A, F960APromoterCMVAvailable sinceMarch 6, 2013AvailabilityAcademic Institutions and Nonprofits only -
pmyc-GFP-TNRC6A-del.GW-I+II-NES-mut
Plasmid#42039DepositorInsertTNRC6A (TNRC6A Human)
UseTagsEGFP and MycExpressionMammalianMutationdeleted aa457-532 and aa716-772, F951A, F955A, I9…PromoterCMVAvailable sinceMarch 6, 2013AvailabilityAcademic Institutions and Nonprofits only -
CJ64 (miR-1003 5' splice site mut)
Plasmid#17785DepositorInsertdme-miR-1003 minigene 5' splice mut
UseDrosophila p-element vectorTagsExpressionMutation5' splice site mut + compsensatory stemPromoterAvailable sinceMay 12, 2008AvailabilityAcademic Institutions and Nonprofits only -
CJ45 (miR-1003 5' splice site mut)
Plasmid#17775DepositorInsertdme-miR-1003 minigene 5' splice mut
UseDrosophila metallothionein gene promoter drives e…TagsExpressionInsectMutation5' splice site mut + compsensatory stemPromoterAvailable sinceApril 15, 2008AvailabilityAcademic Institutions and Nonprofits only -
Mutant ornithine transcarbamylase aa 30-114 deleted (OTC-delta)
Plasmid#71878PurposeFor mitochondrial unfolded protein response researchDepositorInsertOTC-delta (Otc Rat)
UseTagsExpressionMutationDeleted amino acid 30-114 from full length OTCPromoterAvailable sinceJan. 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pETM6-At4CL-PhCHS-CmCHI (C5 mutant, pFlavo-opt)
Plasmid#73404PurposeExpresses three enzymes to convert phenylpropanoic acids to flavanones in vivoDepositorInsertsAt4CL
PhCHS
CmCHI
UseTagsExpressionBacterialMutationPromoterT7Available sinceMarch 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
19ag-SOD2-3xHA-TALE(RNR2-Left)-MutH
Plasmid#209294PurposemitoBE expression vector, target mtRNR2 gene.DepositorInsertSOD2-3xHA-TALE(RNR2-Left)-MutH
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
Mammalian expression: Z-lock cofilin dark state mutant
Plasmid#141136Purposea control for optogenetic cofilin analogDepositorInsertZdk2, Cofilin, LOV2
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTRIPZ Myc2-mouse TTP aa S52 S178 MUT
Plasmid#107001PurposeExpresses epitope-tagged double-mutant murine TTPDepositorInserttristetraprolin (Zfp36 Mouse)
UseTagsDouble Myc peptide epitope tagExpressionMammalianMutationChanged Ser52 to Ala and Ser178 to AlaPromoterAvailable sinceMarch 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
E. coli expression: Z-lock cofilin dark state mutant
Plasmid#141135Purposea control for optogenetic cofilin analogDepositorInsertZdk2, Cofilin, LOV2
UseTagsExpressionBacterialMutationD3 deletion, I32F in Zdk2PromoterAvailable sinceJan. 27, 2021AvailabilityAcademic Institutions and Nonprofits only