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Showing: 1561 - 1600 of 15192 results

  3. SunTagng14aa

    Plasmid
    #106439
    Purpose
    Encodes a SunTag CRISPR cas9 system that does not contain a guide RNA and therefore, does not target the TET1 catalytic domain to any specific region of the genome.
    Depositor
    Steven Jacobsen
    Article
    Gallego-Bartolome et al Proc Natl Acad Sci U S A. 2018 Feb 27;11
    Insert
    NOS_TET1CD_2xNLS_1xHA__sfGFP_scFv_UBQ10_INSULATOR_UBQ10_dCAS9_1xHA_3xNLS_10xGCN414aa_OCS
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available since
    March 14, 2018
    Availability
    Academic Institutions and Nonprofits only
    106439-plasmid-map-sequence-id-205526

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  6. pBUN501

    Plasmid
    #50582
    Purpose
    CRISPR/Cas based plant genome editing and gene regulation; expresses zCas9D10A, gRNA scaffold for insertion of target sequence (AtU6-26 promoter), Bar resistance
    Depositor
    Qi-Jun Chen
    Article
    Xing et al BMC Plant Biol. 2014 Nov 29;14(1):327.
    Inserts
    zCas9D10A
    gRNA scaffold
    Use
    CRISPR; Plant expression
    Tags
    3xFLAG and NLS
    Expression
    Mutation
    Cas9D10A nickase, was derived from the zCas9 and …
    Promoter
    AtU6-26p and Ubi1p
    Available since
    Dec. 11, 2014
    Availability
    Academic Institutions and Nonprofits only
    50582-plasmid-map-sequence-id-75642

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  9. pdCas9 (GB1079)

    Plasmid
    #75399
    Purpose
    Provides the human codon optimized CDS of Cas9 protein with mutated (D10A, H840A) and inactivated catalytic domains as a level 0 GoldenBraid part for C-terminal fusions
    Depositor
    Diego Orzaez
    Article
    Vazquez-Vilar et al Plant Methods. 2016 Feb 1;12:10. doi: 10
    Insert
    Cas9 coding region with mutated (D10A, H840A) and inactivated catalytic domains (human codon optimised)
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available since
    Aug. 5, 2016
    Availability
    Academic Institutions and Nonprofits only
    75399-plasmid-map-sequence-id-151817

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  10. mitoTALECD for_atp1_1397CN

    Plasmid
    #186203
    Purpose
    For genome editing (base editing) of Arabidopsis mitochondria, targeted C of RNA editing site of ATP1 by OTP87
    Depositor
    Shin-ichi Arimura
    Article
    Nakazato et al Proc Natl Acad Sci U S A. 2022 May 17;11
    Inserts
    mitochondrial targeted TALE left hand with 1397C of Cytidine Deaminase (mitoTALECD)
    mitochondrial targeted TALE right hand with 1397N of Cytidine Deaminase (mitoTALECD)
    Use
    Synthetic Biology; Ti plasmid for plant transform…
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Aug. 1, 2022
    Availability
    Academic Institutions and Nonprofits only
    186203-plasmid-map-sequence-id-368337

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  12. tRNA-gRNA position [M1_n-1] (GB1210)

    Plasmid
    #75411
    Purpose
    tRNA and scaffold for the assembly of GBoligomers for the first position (positon [M1_n-1]) of a polycistronic tRNA-gRNA regulated by the (monocot) U3 promoter (2-part multiplexing)
    Depositor
    Diego Orzaez
    Article
    Vazquez-Vilar et al Plant Methods. 2016 Feb 1;12:10. doi: 10
    Insert
    tRNA-gRNA position [M1_n-1]
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available since
    Aug. 5, 2016
    Availability
    Academic Institutions and Nonprofits only
    75411-plasmid-map-sequence-id-211947

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  14. pEGB2Ω2_SF-35s:hCas9:tNos (GB1103)

    Plasmid
    #75400
    Purpose
    Transcriptional unit for (human codon optimized) Cas9 plant expression driven by the 35S promoter. Specially conceived to be linked with the TU of the kanamycin resistance gene GB1181.
    Depositor
    Diego Orzaez
    Article
    Vazquez-Vilar et al Plant Methods. 2016 Feb 1;12:10. doi: 10
    Insert
    hCas9
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Plant
    Mutation
    Promoter
    35S
    Available since
    Aug. 5, 2016
    Availability
    Academic Institutions and Nonprofits only
    75400-plasmid-map-sequence-id-146044

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  19. tRNA-gRNA position [D1_2] (GB1205)

    Plasmid
    #75406
    Purpose
    tRNA and scaffold for the assembly of GBoligomers for the first position (positon [D1_2]) of a polycistronic tRNA-gRNA regulated by the dicot U6-26 or U6-1 promoter (3-part multiplexing)
    Depositor
    Diego Orzaez
    Article
    Vazquez-Vilar et al Plant Methods. 2016 Feb 1;12:10. doi: 10
    Insert
    tRNA-gRNA
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available since
    Aug. 5, 2016
    Availability
    Academic Institutions and Nonprofits only
    75406-plasmid-map-sequence-id-146004

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  21. pAAV.BC(p1-10)-hDLXI56i-minBG-CI-EGFP-W3SL-BC(p1-10)

    Plasmid
    #231360
    Purpose
    Single stranded AAV genome with concatemerization-dependent barcodes, for tracking AAV concatemers via SpECTr. Also expresses EGFP from the minBG promoter with hDLXI56i enhancer.
    Depositor
    Viviana Gradinaru
    Article
    Coughlin et al Nat Biotechnol. 2025 Mar 20. doi: 10.103
    Insert
    BC(p1-10)
    Use
    AAV
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 20, 2025
    Availability
    Academic Institutions and Nonprofits only
    231360-plasmid-map-sequence-id-459858

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  27. pAAV.T7-SP6-BC(p11-14)-SCP1-tdTomato-W3SL-T7

    Plasmid
    #231356
    Purpose
    Single stranded AAV genome with barcodeless T7 RNA polymerase promoter and SP6-driven barcode, for tracking AAV concatemers via SpECTr. Also expresses tdTomato from SCP1 promoter.
    Depositor
    Viviana Gradinaru
    Article
    Coughlin et al Nat Biotechnol. 2025 Mar 20. doi: 10.103
    Insert
    T7-SP6-BC(p11-14)
    Use
    AAV
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 20, 2025
    Availability
    Academic Institutions and Nonprofits only
    231356-plasmid-map-sequence-id-459839

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  31. mitoTALECD for_atp1_1333NC

    Plasmid
    #186202
    Purpose
    For genome editing (base editing) of Arabidopsis mitochondria, targeted C of RNA editing site of ATP1 by OTP87
    Depositor
    Shin-ichi Arimura
    Article
    Nakazato et al Proc Natl Acad Sci U S A. 2022 May 17;11
    Inserts
    mitochondrial targeted TALE left hand with 1333N of Cytidine Deaminase (mitoTALECD)
    mitochondrial targeted TALE right hand with 1333C of Cytidine Deaminase (mitoTALECD)
    Use
    Synthetic Biology; Ti plasmid for plant transform…
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Jan. 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    186202-plasmid-map-sequence-id-380568

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  32. mitoTALECD for_atp1_1397NC

    Plasmid
    #186204
    Purpose
    For genome editing (base editing) of Arabidopsis mitochondria, targeted C of RNA editing site of ATP1 by OTP87
    Depositor
    Shin-ichi Arimura
    Article
    Nakazato et al Proc Natl Acad Sci U S A. 2022 May 17;11
    Inserts
    mitochondrial targeted TALE left hand with 1397N of Cytidine Deaminase (mitoTALECD)
    mitochondrial targeted TALE right hand with 1397C of Cytidine Deaminase (mitoTALECD)
    Use
    Synthetic Biology; Ti plasmid for plant transform…
    Tags
    Expression
    Mutation
    Promoter
    Arabidopsis RPS5A
    Available since
    Aug. 1, 2022
    Availability
    Academic Institutions and Nonprofits only
    186204-plasmid-map-sequence-id-368339

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  33. mitoTALECD for_atp1_1333CN

    Plasmid
    #186201
    Purpose
    For genome editing (base editing) of Arabidopsis mitochondria, targeted C of RNA editing site of ATP1 by OTP87
    Depositor
    Shin-ichi Arimura
    Article
    Nakazato et al Proc Natl Acad Sci U S A. 2022 May 17;11
    Inserts
    mitochondrial targeted TALE left hand with 1333C of Cytidine Deaminase (mitoTALECD)
    mitochondrial targeted TALE right hand with 1333B of Cytidine Deaminase (mitoTALECD)
    Use
    Synthetic Biology; Ti plasmid for plant transform…
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Aug. 1, 2022
    Availability
    Academic Institutions and Nonprofits only
    186201-plasmid-map-sequence-id-368308

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  34. tRNA-gRNA position [M1_2] (GB1209)

    Plasmid
    #75410
    Purpose
    tRNA and scaffold for the assembly of GBoligomers for the first position (positon [M1_2]) of a polycistronic tRNA-gRNA regulated by the (monocot) U3 promoter (3-part multiplexing)
    Depositor
    Diego Orzaez
    Article
    Vazquez-Vilar et al Plant Methods. 2016 Feb 1;12:10. doi: 10
    Insert
    tRNA-gRNA position [M1_2]
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available since
    Aug. 5, 2016
    Availability
    Academic Institutions and Nonprofits only
    75410-plasmid-map-sequence-id-146027

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Showing: 1561 - 1600 of 15192 results