Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

More Filters

We narrowed to 5,811 results for: 129

Clear keywords
Showing: 1951 - 2000 of 5811 results

  5. CC5

    Plasmid
    #63717
    Purpose
    Voltage sensing domain of Ciona with the D129N/D164N/G187A/V220T mutations fused to super ecliptic pHlorin A227D
    Depositor
    Bradley Baker
    Article
    Piao et al J Neurosci. 2015 Jan 7;35(1):372-85. doi
    Insert
    CC5
    Tags
    Super ecliptic pHlorin A227D
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    July 27, 2015
    Availability
    Academic Institutions and Nonprofits only

  6. CC4

    Plasmid
    #63716
    Purpose
    Voltage sensing domain of Ciona with the D129S/R168A/D186S/R229I mutations fused to super ecliptic pHlorin A227D
    Depositor
    Bradley Baker
    Article
    Piao et al J Neurosci. 2015 Jan 7;35(1):372-85. doi
    Insert
    CC4
    Tags
    Super ecliptic pHlorin A227D
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    July 27, 2015
    Availability
    Academic Institutions and Nonprofits only

  7. pET-Ce25

    Plasmid
    #51296
    Purpose
    Expresses a plant arginyl-tRNA synthetase in E.coli
    Depositor
    Gabor Igloi
    Article
    Hogg et al Protein Expr Purif. 2008 Oct;61(2):163-7
    Insert
    arginyl-tRNA synthetase
    Tags
    His Tag and Thioredoxin
    Expression
    Bacterial
    Mutation
    GTT (Val) insertion after initiation codon to per…
    Promoter
    T7
    Available Since
    July 31, 2014
    Availability
    Academic Institutions and Nonprofits only
    51296-plasmid-map-sequence-id-78029

    Enlarge

  9. pYSD313

    Plasmid
    #212931
    Purpose
    Encodes the Aga2 signal peptide, mRuby2 fluorescent protein HA-tagged Sed1p yeast surface display anchor protein with pTEF1 promoter, tTDH1 terminator, LEU2 selectable marker and CEN/ARS yeast origin.
    Depositor
    Paul Young
    Article
    O'Riordan et al ACS Synth Biol. 2024 Mar 14. doi: 10.102
    Insert
    Sed1 (SED1 Budding Yeast)
    Expression
    Yeast
    Available Since
    Feb. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    212931-plasmid-map-sequence-id-421768

    Enlarge

  14. pLKO5.U6.DR130(CasRX)_Empty.EFS.tRFP657

    Plasmid
    #212961
    Purpose
    Lentiviral plasmid for the cloning of mature gRNAs of the RfxCas13d (CasRx) CRISPR system. As a marker, the plasmid also encodes the tRFP657 fluorescence protein.
    Depositor
    Roland Rad
    Article
    Montero et al Nat Methods. 2024 Feb 26. doi: 10.1038/s
    Insert
    DR1_CasRx_backbone
    Use
    CRISPR and Lentiviral
    Tags
    TagRFP657
    Expression
    Mammalian
    Promoter
    U6
    Available Since
    Feb. 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    212961-plasmid-map-sequence-id-422482

    Enlarge

  15. pLKO5.U6.DR130(CasRX)_Non-targeting sgRNA.EFS.tRFP657

    Plasmid
    #212962
    Purpose
    Lentiviral plasmid for the measurement of RfxCas13d (CasRx) nuclease activity. As a marker, the plasmid also encodes the tRFP657 fluorescence protein.
    Depositor
    Roland Rad
    Article
    Montero et al Nat Methods. 2024 Feb 26. doi: 10.1038/s
    Insert
    DR1_CasRx_non-targeting spacer
    Use
    CRISPR and Lentiviral
    Tags
    TagRFP657
    Expression
    Mammalian
    Promoter
    U6
    Available Since
    Feb. 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    212962-plasmid-map-sequence-id-422485

    Enlarge

  16. pLKO5.U6.DR130(CasRX)_GFP-targeting sgRNA.EFS.tRFP657

    Plasmid
    #212963
    Purpose
    Lentiviral plasmid for the measurement of RfxCas13d (CasRx) nuclease activity. As a marker, the plasmid also encodes the tRFP657 fluorescence protein.
    Depositor
    Roland Rad
    Article
    Montero et al Nat Methods. 2024 Feb 26. doi: 10.1038/s
    Insert
    DR1_CasRx_GFP targeting spacer
    Use
    CRISPR and Lentiviral
    Tags
    TagRFP657
    Expression
    Mammalian
    Promoter
    U6
    Available Since
    Feb. 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    212963-plasmid-map-sequence-id-422499

    Enlarge

  24. pmIL-6 mut C/EBP

    Plasmid
    #61291
    Purpose
    drives luciferase from mouse IL-6 promoter with mutant C/EBP (NF-IL-6) site
    Depositor
    Gail Bishop
    Article
    Baccam et al J Immunol. 2003 Mar 15;170(6):3099-108.
    Insert
    IL-6 promoter (Il6 Mouse)
    Use
    Luciferase
    Expression
    Mammalian
    Mutation
    Mutated C/EBP binding site from ACATTGTGCAATCT to…
    Promoter
    IL-6 promoter with mutant C/EBP binding site
    Available Since
    May 4, 2015
    Availability
    Academic Institutions and Nonprofits only

  32. pETSUMO2_Rhodoplanes-bGSDM

    Plasmid
    #212129
    Purpose
    For expression of the bacterial gasdermin (bGSDM) encoded by a Rhodoplanes species. Protein is expressed with an N-terminal SUMO2 tag.
    Depositor
    Philip Kranzusch
    Article
    Johnson et al Science. 2022 Jan 14;375(6577):221-225.
    Insert
    Rhodoplanes bGSDM
    Tags
    6xHis-SUMO2
    Expression
    Bacterial
    Mutation
    N-terminal methionine deleted and replaced by sin…
    Promoter
    T7
    Available Since
    Jan. 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    212129-plasmid-map-sequence-id-419110

    Enlarge

  44. JPZF10 CMV-TO-SCN1A-372-NZF

    Plasmid
    #236171
    Purpose
    Plasmid encoding the SCN1A-372 zinc finger array attached by a GS linker to the NZF transcriptional activation domain, under control of CMV promoter with two TetR binding sites
    Depositor
    Xiaojing Gao
    Article
    Wolfsberg et al Cell Syst. 2025 Jul 16;16(7):101299. doi
    Insert
    SCN1A-372-NZF
    Expression
    Mammalian
    Promoter
    CMV promoter with two TetR binding sites
    Available Since
    May 6, 2025
    Availability
    Academic Institutions and Nonprofits only
    236171-plasmid-map-sequence-id-471374

    Enlarge

  45. EW172 CMV-TO-UTRN-14ZF-VP64 (FLP-IN)

    Plasmid
    #236160
    Purpose
    Plasmid encoding the UTRN-14 zinc finger array attached by a GS linker to the VP64 transcriptional activation domain, under control of CMV promoter with two TetR binding sites
    Depositor
    Xiaojing Gao
    Article
    Wolfsberg et al Cell Syst. 2025 Jul 16;16(7):101299. doi
    Insert
    UTRN-14ZF-VP64
    Expression
    Mammalian
    Promoter
    CMV promoter with two TetR binding sites
    Available Since
    April 9, 2025
    Availability
    Academic Institutions and Nonprofits only
    236160-plasmid-map-sequence-id-468466

    Enlarge

  46. EW215 PGK-ZNF35(5-8)-deImmunLink-NZF(FLP-IN)

    Plasmid
    #236130
    Purpose
    Plasmid encoding the ZNF35-derived portion of the ZNF35/ZNF250 zinc finger array attached by a deimmunized linker to the NZF transcriptional activation domain, under control of PGK promoter
    Depositor
    Xiaojing Gao
    Article
    Wolfsberg et al Cell Syst. 2025 Jul 16;16(7):101299. doi
    Insert
    ZNF35(5-8)-deImmunLink-NZF
    Expression
    Mammalian
    Promoter
    PGK
    Available Since
    April 9, 2025
    Availability
    Academic Institutions and Nonprofits only
    236130-plasmid-map-sequence-id-468462

    Enlarge

  47. JPZF4 CMV-TO-SCN1A-383-NZF

    Plasmid
    #236165
    Purpose
    Plasmid encoding the SCN1A-383 zinc finger array attached by a GS linker to the NZF transcriptional activation domain, under control of CMV promoter with two TetR binding sites
    Depositor
    Xiaojing Gao
    Article
    Wolfsberg et al Cell Syst. 2025 Jul 16;16(7):101299. doi
    Insert
    SCN1A-383-NZF
    Expression
    Mammalian
    Promoter
    CMV promoter with two TetR binding sites
    Available Since
    April 9, 2025
    Availability
    Academic Institutions and Nonprofits only
    236165-plasmid-map-sequence-id-468463

    Enlarge

  48. JPZF5 CMV-TO-SCN1A-472-NZF

    Plasmid
    #236166
    Purpose
    Plasmid encoding the SCN1A-472 zinc finger array attached by a GS linker to the NZF transcriptional activation domain, under control of CMV promoter with two TetR binding sites
    Depositor
    Xiaojing Gao
    Article
    Wolfsberg et al Cell Syst. 2025 Jul 16;16(7):101299. doi
    Insert
    SCN1A-472-NZF
    Expression
    Mammalian
    Promoter
    CMV promoter with two TetR binding sites
    Available Since
    April 9, 2025
    Availability
    Academic Institutions and Nonprofits only
    236166-plasmid-map-sequence-id-468465

    Enlarge

  49. EW196 PGK-ZNF35(5-8)-ZNF250(1-4)-deImmunLink-NZF(FLP-IN)

    Plasmid
    #236132
    Purpose
    Plasmid encoding the ZNF35/ZNF250 zinc finger array attached by a deimmunized linker to the NZF transcriptional activation domain, under control of PGK promoter
    Depositor
    Xiaojing Gao
    Article
    Wolfsberg et al Cell Syst. 2025 Jul 16;16(7):101299. doi
    Insert
    ZNF35(5-8)-ZNF250(1-4)-deImmunLink-NZF
    Expression
    Mammalian
    Promoter
    PGK
    Available Since
    April 9, 2025
    Availability
    Academic Institutions and Nonprofits only
    236132-plasmid-map-sequence-id-468459

    Enlarge

  50. EW269 CMV-TO-Gal4-NZF[GS Linkers] (FLP-IN)

    Plasmid
    #236122
    Purpose
    Plasmid encoding Gal4-NZF transcription factor with GS linkers between the component domains of NZF, under control of CMV promoter with two TetR binding sites
    Depositor
    Xiaojing Gao
    Article
    Wolfsberg et al Cell Syst. 2025 Jul 16;16(7):101299. doi
    Insert
    Gal4-NZF (GS Linkers)
    Expression
    Mammalian
    Promoter
    CMV promoter with two TetR binding sites
    Available Since
    April 9, 2025
    Availability
    Academic Institutions and Nonprofits only
    236122-plasmid-map-sequence-id-468454

    Enlarge

Showing: 1951 - 2000 of 5811 results