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K785R Smarca4-V5 IRES-puro

Plasmid

#107059

Purpose

Expression of dominant-negative K785R Smarca4 (Brg1)-V5 fusion with IRES puromycin resistance.

Depositor

Jerry Crabtree, Courtney Hodges

Article

Hodges et al Nat Struct Mol Biol. 2018 Jan;25(1):61-7

Insert

SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 4 (Smarca4 Mouse)

Use

Lentiviral

Tags

IRES-puroR and V5

Expression

Mammalian

Mutation

K785R

Promoter

CAG

Available since

April 3, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-R-iLACCO1.1

Plasmid

#208100

Purpose

Biosensor for intracellular L-lactate

Depositor

Robert Campbell, Marie-Eve Paquet

Article

Nasu et al Nat Commun. 2023 Oct 27;14(1):6598. doi:

Insert

R-iLACCO1.2

Use

AAV

Tags

Expression

Mutation

Promoter

Available since

Nov. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA-HA-deLACCO1-NGR

Plasmid

#208024

Purpose

Control biosensor for extracellular L-lactate

Depositor

Robert Campbell

Article

Nasu et al Nat Commun. 2023 Oct 27;14(1):6598. doi:

Insert

deLACCO1

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 30, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-gfaABC1D-R-iLACCO1.1

Plasmid

#208103

Purpose

Biosensor for intracellular L-lactate

Depositor

Robert Campbell, Marie-Eve Paquet

Article

Nasu et al Nat Commun. 2023 Oct 27;14(1):6598. doi:

Insert

deLACCO1

Use

AAV

Tags

Expression

Mutation

Promoter

Available since

Nov. 14, 2023

Availability

Academic Institutions and Nonprofits only

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LentiCRISPR v2-sgAMPK alpha2 clone1

Plasmid

#162122

Purpose

Lentiviral sgRNA plasmid targeting human AMPK alpha2

Depositor

Boyi Gan

Article

Lee et al Nat Cell Biol. 2020 Feb;22(2):225-234. d

Insert

sgAMPK alpha2 (PRKAA2 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Jan. 26, 2021

Availability

Academic Institutions and Nonprofits only

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BPK1520-LRRK2-G2019S-ng

Plasmid

#180436

Purpose

Transiently expressing a nicking sgRNA to facilitate the introduction of LRRK2-G2019S mutation in PE3 approach

Depositor

Frank Soldner

Article

Li et al Elife. 2022 Sep 7;11:e79208. doi: 10.755

Insert

ncRNA targeting LRRK2-G2019S (LRRK2 Human)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pU6-pegRNA-SNCA-A53T

Plasmid

#181738

Purpose

Transiently expressing a pegRNA to introduce SNCA-A53T mutation in human cells

Depositor

Dirk Hockemeyer

Article

Li et al Elife. 2022 Sep 7;11:e79208. doi: 10.755

Insert

Prime editing pegRNA for SNCA-A53T (SNCA Synthetic)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Human U6

Available since

Sept. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pBPK1520-SNCA-A53T-ng

Plasmid

#181739

Purpose

Transiently expressing a nicking sgRNA to facilitate the introduction of SNCA-A53T mutation in PE3 approach

Depositor

Dirk Hockemeyer

Article

Li et al Elife. 2022 Sep 7;11:e79208. doi: 10.755

Insert

Prime editing ngRNA for SNCA-A53T (SNCA Human)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Human U6

Available since

Sept. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pBPK1520-HEK3-CTTins-ng

Plasmid

#180019

Purpose

Transiently expressing a nicking sgRNA to facilitate the introduction of HEK3 CTT insertion in PE3 approach

Depositor

Dirk Hockemeyer

Article

Li et al Elife. 2022 Sep 7;11:e79208. doi: 10.755

Insert

Prime editing ngRNA for HEK3-CTTins (EPHA8 Human)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Human U7

Available since

Sept. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pU6-pegRNA-SNCA-A30P

Plasmid

#180016

Purpose

Transiently expressing a pegRNA to introduce SNCA-A30P mutation in human cells

Depositor

Dirk Hockemeyer

Article

Li et al Elife. 2022 Sep 7;11:e79208. doi: 10.755

Insert

Prime editing pegRNA for SNCA-A30P (SNCA Synthetic)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Human U6

Available since

Sept. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pBPK1520-SNCA-A30P-ng

Plasmid

#180018

Purpose

Transiently expressing a nicking sgRNA to facilitate the introduction of SNCA-A30P mutation in PE3 approach

Depositor

Dirk Hockemeyer

Article

Li et al Elife. 2022 Sep 7;11:e79208. doi: 10.755

Insert

Prime editing ngRNA for SNCA-A30P (SNCA Human)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Human U6

Available since

Sept. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pBAD-R-iLACCO1.1

Plasmid

#208020

Purpose

Biosensor for intracellular L-lactate

Depositor

Robert Campbell

Article

Nasu et al Nat Commun. 2023 Oct 27;14(1):6598. doi:

Insert

R-iLACCO1.1

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 30, 2023

Availability

Academic Institutions and Nonprofits only

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FLAG-Mkk7-JNK2 fusion-pcw107

Plasmid

#64618

Purpose

Mkk7-JNK2 fusion - barcoded activating construct of JNK pathway

Depositor

Kris Wood, David Sabatini

Article

Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

Insert

Map2k7 linker MAPK9

Use

Lentiviral

Tags

FLAG

Expression

Mutation

mouse-human fusion

Promoter

PGK

Available since

Dec. 21, 2015

Availability

Academic Institutions and Nonprofits only
LentiCRISPR v2-sgACSL4 clone2

Plasmid

#162129

Purpose

Lentiviral sgRNA plasmid targeting human ACSL4

Depositor

Boyi Gan

Article

Lee et al Nat Cell Biol. 2020 Feb;22(2):225-234. d

Insert

sgACSL4 (ACSL4 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Feb. 9, 2021

Availability

Academic Institutions and Nonprofits only

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LentiCRISPR v2-sgACSL4 clone1

Plasmid

#162128

Purpose

Lentiviral sgRNA plasmid targeting human ACSL4

Depositor

Boyi Gan

Article

Lee et al Nat Cell Biol. 2020 Feb;22(2):225-234. d

Insert

sgACSL4 (ACSL4 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Feb. 9, 2021

Availability

Academic Institutions and Nonprofits only

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pT7-7 asyn S87E

Plasmid

#36059

Depositor

Hilal Lashuel

Article

Paleologou et al J Neurosci. 2010 Mar 3;30(9):3184-98.

Insert

alpha-synuclein S87E (SNCA Human)

Use

Tags

Expression

Bacterial

Mutation

S87E

Promoter

T7

Available since

July 13, 2012

Availability

Academic Institutions and Nonprofits only

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LentiCRISPR v2-sgACSL4 clone3

Plasmid

#162130

Purpose

Lentiviral sgRNA plasmid targeting human ACSL4

Depositor

Boyi Gan

Article

Lee et al Nat Cell Biol. 2020 Feb;22(2):225-234. d

Insert

sgACSL4 (ACSL4 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Feb. 9, 2021

Availability

Academic Institutions and Nonprofits only

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pT7-7 asyn S87A

Plasmid

#36058

Depositor

Hilal Lashuel

Article

Paleologou et al J Neurosci. 2010 Mar 3;30(9):3184-98.

Insert

alpha-synuclein S87A (SNCA Human)

Use

Tags

Expression

Bacterial

Mutation

S87A

Promoter

T7

Available since

July 13, 2012

Availability

Academic Institutions and Nonprofits only

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RalA (G23V)( full )-pcw107-V5

Plasmid

#64645

Purpose

(control) when used to produce lentivirus, express physiological levels of insert

Depositor

Kris Wood, David Sabatini

Article

Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

Insert

RALA (RALA Human)

Use

Lentiviral

Tags

V5

Expression

Mutation

G23V

Promoter

PGK

Available since

Dec. 1, 2015

Availability

Academic Institutions and Nonprofits only
RalA (G23V)( full )-pcw107

Plasmid

#64644

Purpose

when used to produce lentivirus, express physiological levels of insert

Depositor

Kris Wood, David Sabatini

Article

Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

Insert

RALA (RALA Human)

Use

Lentiviral

Tags

Expression

Mutation

G23V

Promoter

PGK

Available since

Dec. 1, 2015

Availability

Academic Institutions and Nonprofits only