We narrowed to 835 results for: PC1;

Showing: 781 - 800 of 835 results

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TMEM138-mCherry

Plasmid

#41630

Depositor

Joseph Gleeson

Article

Lee et al Science. 2012 Feb 24;335(6071):966-9. Ep

Insert

Transmembrane Protein 138 (TMEM138 Human)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

April 24, 2013

Availability

Academic Institutions and Nonprofits only
pcDNA8-Flag-LARS(1-360aa)

Plasmid

#139668

Purpose

Expresses N-teminal Flag tagged aa 1-360 of LARS1 protein

Depositor

Jie Chen

Article

Yoon et al Cell Rep. 2016 Aug 9;16(6):1510-1517. do

Insert

Leucyl-tRNA Synthetase (LARS1 Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

April 24, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA TMEM 138-flag

Plasmid

#41628

Depositor

Joseph Gleeson

Article

Lee et al Science. 2012 Feb 24;335(6071):966-9. Ep

Insert

Transmembrane Protein 138 (TMEM138 Human)

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available Since

April 24, 2013

Availability

Academic Institutions and Nonprofits only
pCA528 CHMP5 159-222

Plasmid

#108259

Purpose

Expresses C-terminal 159-222 residues of CHMP5 with non-native N-term cysteine for fluorescent labeling; Internal ID: WISP18-10

Depositor

Wesley Sundquist

Article

Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

Insert

CHMP5 residues 148-219 (CHMP5 Human)

Tags

His-SUMO

Expression

Bacterial

Available Since

Nov. 20, 2024

Availability

Academic Institutions and Nonprofits only

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CL20_mEGFP_PICK1_POLR2F

Plasmid

#205894

Purpose

Express mEGFP-tagged fusion protein, PICK1_POLR2F from patient-derived sequence

Depositor

Richard Kriwacki

Article

Tripathi et al Nat Commun. 2023 Sep 28;14(1):6008. doi:

Insert

PICK1_POLR2F (PICK1, POLR2F Human)

Tags

mEGFP

Expression

Mammalian

Available Since

Nov. 9, 2023

Availability

Academic Institutions and Nonprofits only

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PGK EGFP-LC3B

Plasmid

#200427

Purpose

Mammalian expression of fluorescently-tagged marker for autophagic vesicles

Depositor

Erika Holzbaur

Article

Dou et al Cell Rep. 2023 May 1;42(5):112448. doi:

Insert

MAP1LC3B (MAP1LC3B Human)

Tags

EGFP

Expression

Mammalian

Promoter

PGK

Available Since

May 22, 2023

Availability

Academic Institutions and Nonprofits only

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pET28a Cdk2ap1ΔN (MT2B2)-MutTER

Plasmid

#178035

Purpose

Expression vector for the purpose of protein purification.

Depositor

Lin He

Article

Modzelewski et al Cell. 2021 Oct 7. pii: S0092-8674(21)011

Insert

Cdk2ap1 (Cdk2ap1 Mouse)

Use

Nonviral

Tags

6xHIS - Thrombin - MBP- TEV-TRS

Expression

Bacterial

Mutation

First N-Terminal 27aa are deleted and Changed T(8…

Promoter

T7LacI

Available Since

Nov. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pET28a Cdk2ap1CAN-MutTER

Plasmid

#178033

Purpose

Expression vector for the purpose of protein purification.

Depositor

Lin He

Article

Modzelewski et al Cell. 2021 Oct 7. pii: S0092-8674(21)011

Insert

Cdk2ap1 (Cdk2ap1 Mouse)

Use

Nonviral

Tags

6xHIS - Thrombin - MBP- TEV-TRS

Expression

Bacterial

Mutation

Changed T(108)A, E(109)A, R(110)A

Promoter

T7LacI

Available Since

Nov. 16, 2022

Availability

Academic Institutions and Nonprofits only

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pET28a Cdk2ap1ΔN (MT2B2)

Plasmid

#178034

Purpose

Expression vector for the purpose of protein purification.

Depositor

Lin He

Article

Modzelewski et al Cell. 2021 Oct 7. pii: S0092-8674(21)011

Insert

Cdk2ap1 (Cdk2ap1 Mouse)

Use

Nonviral

Tags

6xHIS - Thrombin - MBP- TEV-TRS

Expression

Bacterial

Mutation

First N-Terminal 27aa are deleted

Promoter

T7LacI

Available Since

Feb. 4, 2022

Availability

Academic Institutions and Nonprofits only

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psiCheck Cdk2ap1ΔN (MT2B2)

Plasmid

#178029

Purpose

expression vector to test translation efficiency by luciferase reporter.

Depositor

Lin He

Article

Modzelewski et al Cell. 2021 Oct 7. pii: S0092-8674(21)011

Insert

Cdk2ap1 (Cdk2ap1 Mouse)

Use

Luciferase; Nonviral

Tags

Luciferase

Expression

Mammalian

Promoter

Cloned in

Available Since

Jan. 12, 2022

Availability

Academic Institutions and Nonprofits only

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pAW-BiP-NbVHH05-mCherry-KDEL

Plasmid

#171574

Purpose

Expresses mCherry-tagged anti-UBC6e (human) recombinant alpaca nanobody (NbVHH05-mCherry) in fly cell ER membrane

Depositor

Norbert Perrimon, Hidde Ploegh

Article

Xu et al Elife. 2022 Jan 25;11. pii: 74326. doi:

Insert

BiP-NbVHH05-mCherry-KDEL (UBE2J1 Human)

Tags

BiP signal peptide and mCherry-KDEL

Expression

Insect

Promoter

fly actin5C promoter

Available Since

June 29, 2021

Availability

Academic Institutions and Nonprofits only

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pDONR223-ZNF581

Plasmid

#88157

Purpose

Donor Vector containing ZNF581 transcription factor, part of the Human TFome Collection

Depositor

Marc Vidal

Article

Ng et al Nat Biotechnol. 2021 Apr;39(4):510-519.

Insert

ZNF581 (ZNF581 Human)

Use

Gateway donor vector

Mutation

Last nucleotide of stop codon removed to allow fo…

Available Since

June 3, 2021

Availability

Academic Institutions and Nonprofits only

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pInducer10-SAE1_sh3

Plasmid

#168985

Purpose

Expresses RFP cDNA and SAE1 shRNA 3 in mammalian cells

Depositor

Ji Luo

Article

Yu et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

Insert

SUMO1 Activating Enzyme Subunit 1 (SAE1 Human)

Expression

Mammalian

Promoter

Ubc promoter

Available Since

June 1, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA8-Flag-LARS(1-720aa)

Plasmid

#139687

Purpose

Expresses N-teminal Flag tagged aa 1- 720 of LARS1

Depositor

Jie Chen

Article

Yoon et al Cell Rep. 2016 Aug 9;16(6):1510-1517. do

Insert

LARS1 (LARS1 Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

July 31, 2020

Availability

Academic Institutions and Nonprofits only

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TFORF3505

Plasmid

#144981

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

KLF12 (KLF12 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Promoter

EF1a

Available Since

Jan. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pQhsCDtRNA-

Plasmid

#86015

Purpose

Multi-gene expression system with human box C/D proteins with U14

Depositor

Hong Li

Article

Peng et al PLoS One. 2014 Jul 31;9(7):e103096. doi:

Inserts

U14 (SNORD14A Human)
15.5K (SNU13 Human)
fibrillarin (FBL Human)
NOP56 (NOP56 Human)
NOP58 (NOP58 Human)

Use

Co-expression; purification

Tags

6x-His

Expression

Bacterial

Mutation

amino acids 1-401, amino acids 1-411, and amino a…

Available Since

Jan. 9, 2017

Availability

Academic Institutions and Nonprofits only

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HA-BACE1-mBFP

Plasmid

#196697

Purpose

Expresses Bace1 with an HA tag at N-terminal for single molecule tracking and other measusers in mammalian cells

Depositor

Martino Calamai

Article

Capitini et al iScience. 2023 Apr 11;26(5):106611. doi:

Insert

Beta-secretase 1 (BACE1 Human)

Tags

HA and mBFP

Expression

Mammalian

Available Since

April 26, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV-HA_N-human Prkd2 without kinase domain

Plasmid

#138411

Purpose

Express Prkd2 without kinase domain in mammalian cells

Depositor

Bruce Beutler

Article

Misawa et al Sci Immunol. 2020 Jan 24;5(43):eaaz0085.

Insert

Prkd2 without kinase domain (PRKD2 Human)

Tags

HA

Expression

Mammalian

Mutation

kinase domain deleted from human Prkd2

Promoter

CMV

Available Since

April 3, 2025

Availability

Academic Institutions and Nonprofits only

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Bet1L-mCherry

Plasmid

#209849

Purpose

Expresses Bet1L-mCherry in mammalian cells

Depositor

Geert van den Bogaart

Article

Linders et al Nat Commun. 2021 Oct 28;12(1):6227. doi:

Insert

Bet1L (BET1L Human)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pSA2

Plasmid

#52262

Purpose

Bacterial expression of human 6His-tagged SUMO2 and SUMO-E1

Depositor

Primo Schaer

Article

Weber et al PLoS One. 2014 Jul 9;9(7):e102157. doi:

Inserts

SUMO-2 (SUMO2 Human)
SAE-2 (UBA2 Human)
SAE-1 (SAE1 Human)

Tags

6His-tag

Expression

Bacterial

Mutation

C-terminal gly-gly

Promoter

T7 and rbs

Available Since

July 10, 2014

Availability

Academic Institutions and Nonprofits only

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