We narrowed to 2,518 results for: PLS

1. pCIG-FGFRdn

   Plasmid

   #80431

   Purpose

   Expression of a dominant-negative FGFR1 with a nuclear EGFP reporter in chick embryos

   Depositor

   Aixa Morales

   Article

   Martinez-Morales et al J Cell Biol. 2011 Aug 8;194(3):489-503.

   Insert

   Fibroblast growth factor receptor 1 (FGFR1 Chicken)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   contains aa 1–425

   Promoter

   beta-actin promoter

   Available Since

   Feb. 28, 2017

   Availability

   Academic Institutions and Nonprofits only

2. FLAG-FOXO3a TM

   Plasmid

   #8361

   Depositor

   Michael Greenberg

   Article

   Brunet et al Science 2004 Mar 26;303(5666):2011-5. Ep

   Insert

   FOXO3a (FOXO3 Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   T32A, S253A, S315A

   Promoter

   Available Since

   Feb. 13, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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3. cpVenus-Gγ2

   Plasmid

   #69626

   Purpose

   The heterotrimeric G-protein subunit, Gγ2, tagged with circular permutated Venus (cp173V).

   Depositor

   Dorus Gadella

   Article

   van Unen et al PLoS One. 2016 Jan 22;11(1):e0146789. do

   Insert

   cp173Venus-Ggamma2 (GNG2 Synthetic)
   

   Use

   Tags

   ciruclar permutated Venus (cp173V)

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Jan. 25, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGL3 basic Mut E2

   Plasmid

   #48748

   Purpose

   Expression of luciferase driven by mPer2 promoter fragment containing mutated E-box2 (bases -112 to +98 with respect to transcription start site at +1)

   Depositor

   Joseph Takahashi

   Article

   Yoo et al Proc Natl Acad Sci U S A. 2005 Feb 15;10

   Insert

   mPer2 promoter/enhancer region containing mutated E-box2 (-112 to +98)
   

   Use

   Luciferase

   Tags

   Expression

   Mutation

   Mutated E-box2 sequence from CACGTT to GCTAGT

   Promoter

   Available Since

   Dec. 5, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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5. pHtrA-TEV-His12

   Plasmid

   #137036

   Purpose

   expression clone from T7 promoter for full-length (signal peptide containing) B. burgdorferi HtrA with C-terminal TEV-protease-cleavable His12

   Depositor

   Debra Hansen

   Article

   Robertson et al Sci Rep. 2019 Nov 26;9(1):17606. doi: 10

   Insert

   AAC66500.2 (BB_0104 Borrelia burgdorferi B31)
   

   Use

   Tags

   His12 (TEV protease cleavable)

   Expression

   Bacterial

   Mutation

   wild type, full-length protein, contains signal p…

   Promoter

   T7

   Available Since

   March 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMSCV-puro-mycRSK2wt

   Plasmid

   #15827

   Depositor

   Jing Chen

   Article

   Kang et al Cancer Cell. 2007 Sep . 12(3):201-14.

   Insert

   Ribosomal S6 kinase 2 (Rps6ka3 Mouse)
   

   Use

   Retroviral

   Tags

   myc

   Expression

   Mammalian

   Mutation

   wild type

   Promoter

   Available Since

   Nov. 5, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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7. PB-Neo-TetO-Cdk12_Nterm_FlagHa

   Plasmid

   #127179

   Purpose

   N-terminal Flag- HA- tandem epitope tagged mouse Cdk12 transgene (NM_001109626.1 isoform) cloned into a dox-inducible piggybac destination vector

   Depositor

   Phil Sharp

   Article

   Dubbury et al Nature. 2018 Dec;564(7734):141-145. doi:

   Insert

   N-terminally Flag- HA- Epitope Tagged Mouse Cyclin Dependent Kinase 12 (Cdk12 Mouse)
   

   Use

   Piggybac transposase vector

   Tags

   Flag- HA- Tandem Epitopes

   Expression

   Mammalian

   Mutation

   Promoter

   TetO Promoter

   Available Since

   July 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDipA-TEV-His12

   Plasmid

   #137042

   Purpose

   expression clone from T7 promoter for full-length (signal peptide containing) B. burgdorferi DipA with C-terminal TEV-protease-cleavable His12

   Depositor

   Debra Hansen

   Article

   Robertson et al Sci Rep. 2019 Nov 26;9(1):17606. doi: 10

   Insert

   AAC66790.1 (BB_0418 Borrelia burgdorferi B31)
   

   Use

   Tags

   His12 (TEV protease cleavable)

   Expression

   Bacterial

   Mutation

   wild type, full-length protein, contains signal p…

   Promoter

   T7

   Available Since

   March 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGL3 Pro Mut E2

   Plasmid

   #48750

   Purpose

   Expression of luciferase driven by mPer2 promoter fragment containing mutated E-box2 (bases -112 to +98 with respect to transcription start site at +1) and SV40 promoter (backbone)

   Depositor

   Joseph Takahashi

   Article

   Yoo et al Proc Natl Acad Sci U S A. 2005 Feb 15;10

   Insert

   mPer2 promoter/enhancer region containing mutated E-box2 (-112 to +98)
   

   Use

   Luciferase

   Tags

   Expression

   Mutation

   Mutated E-box2 sequence from CACGTT to GCTAGT

   Promoter

   Available Since

   Dec. 5, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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10. pBBA57-TEV-His12

    Plasmid

    #137033

    Purpose

    expression clone from T7 promoter for full-length (signal peptide containing) B. burgdorferi BBA57 with C-terminal TEV-protease-cleavable His12

    Depositor

    Debra Hansen

    Article

    Robertson et al Sci Rep. 2019 Nov 26;9(1):17606. doi: 10

    Insert

    AAC66270.1 (BB_A57 Borrelia burgdorferi B31)
    

    Use

    Tags

    His12 (TEV protease cleavable)

    Expression

    Bacterial

    Mutation

    wild type, full-length BBA57, contains signal pep…

    Promoter

    T7

    Available Since

    March 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. Luciferase-pcw107

    Plasmid

    #64648

    Purpose

    (control) when used to produce lentivirus, express physiological levels of insert

    Depositor

    David Sabatini, Kris Wood

    Article

    Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

    Insert

    n/a
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    PGK

    Available Since

    April 29, 2015

    Availability

    Academic Institutions and Nonprofits only

12. HcRed-pcw107-V5

    Plasmid

    #64647

    Purpose

    (control) when used to produce lentivirus, express physiological levels of insert

    Depositor

    David Sabatini, Kris Wood

    Article

    Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

    Insert

    n/a
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mutation

    Promoter

    PGK

    Available Since

    July 8, 2015

    Availability

    Academic Institutions and Nonprofits only

13. sTR056

    Plasmid

    #177369

    Purpose

    Toehold switch, with an unpaired toehold region at the 5′-end that interacts with the trigger RNA (sTR060, Addgene plasmid # 177370) to unfold the switch.

    Depositor

    Jerome Bonnet, Jean-Loup Faulon

    Article

    Batista et al ACS Synth Biol. 2022 Jan 16. doi: 10.102

    Insert

    sfGFP
    

    Use

    Synthetic Biology; Cell-free protein synthesis

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Jan. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. HcRed-pcw107

    Plasmid

    #64646

    Purpose

    when used to produce lentivirus, express physiological levels of insert

    Depositor

    David Sabatini, Kris Wood

    Article

    Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

    Insert

    n/a
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    PGK

    Available Since

    May 7, 2015

    Availability

    Academic Institutions and Nonprofits only

15. pBB0405-TEV-His12

    Plasmid

    #137040

    Purpose

    expression clone from T7 promoter for full-length (signal peptide containing) B. burgdorferi BB0405 with C-terminal TEV-protease-cleavable His12

    Depositor

    Debra Hansen

    Article

    Robertson et al Sci Rep. 2019 Nov 26;9(1):17606. doi: 10

    Insert

    AAC66795.1 (BB_0405 Borrelia burgdorferi B31)
    

    Use

    Tags

    His12 (TEV protease cleavable)

    Expression

    Bacterial

    Mutation

    wild type, full-length protein, contains signal p…

    Promoter

    T7

    Available Since

    March 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pRevA-TEV-His12

    Plasmid

    #137034

    Purpose

    expression clone from T7 promoter for full-length (signal peptide containing) B. burgdorferi RevA with C-terminal TEV-protease-cleavable His12

    Depositor

    Debra Hansen

    Article

    Robertson et al Sci Rep. 2019 Nov 26;9(1):17606. doi: 10

    Insert

    AAF07416.1 (revA Borrelia burgdorferi B31)
    

    Use

    Tags

    His12 (TEV protease cleavable)

    Expression

    Bacterial

    Mutation

    wild type, full-length protein, contains signal p…

    Promoter

    T7

    Available Since

    March 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pP13-TEV-His12

    Plasmid

    #137035

    Purpose

    expression clone from T7 promoter for full-length (signal peptide containing) B. burgdorferi P13 with C-terminal TEV-protease-cleavable His12

    Depositor

    Debra Hansen

    Article

    Robertson et al Sci Rep. 2019 Nov 26;9(1):17606. doi: 10

    Insert

    AAC66426.1 (BB_0034 Borrelia burgdorferi B31)
    

    Use

    Tags

    His12 (TEV protease cleavable)

    Expression

    Bacterial

    Mutation

    wild type, full-length protein, contains signal p…

    Promoter

    T7

    Available Since

    March 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. C24m

    Plasmid

    #120964

    Purpose

    MoClo golden gate assembly CD part for lambda cI (repressor of pL or pOR1-OR2; use C64m for temperature-sensitive version). Please see Supplemental Documents for annotated Genbank file.

    Depositor

    Richard Murray

    Insert

    lambda-cI
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Dec. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. TARS_pNIC-Bio3

    Plasmid

    #153057

    Purpose

    Expression construct for producing Avi tagged Threonyl-tRNA synthetase

    Depositor

    Susanne Gräslund

    Insert

    TARS (TARS1 Human)
    

    Use

    Tags

    Avi and His6-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    June 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLentipuro3/TO/V5-GW/EGFP-Firefly Luciferase

    Plasmid

    #119816

    Purpose

    3rd generation lentiviral plasmid for expression of EGFP Firefly Luciferase fusion protein in mammalian cells

    Depositor

    Ethan Abel

    Article

    Lorenzatti Hiles et al Nat Protoc. 2019 Mar;14(3):738-755. doi:

    Inserts

    Luciferase
    Puromycin
    

    Use

    Lentiviral and Luciferase

    Tags

    EGFP and V5

    Expression

    Mammalian

    Mutation

    Stop codon removed in the frame of the V5 frame v…

    Promoter

    CMV/TO and Human Phosphoglycerate kinase

    Available Since

    Jan. 2, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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