We narrowed to 850 results for: nanobodies

1. pHCMV-SS-antiMoonTagNb-TM

   Plasmid

   #198305

   Purpose

   Displays anti-MoonTag nanobody from the surface of mammalian cells

   Depositor

   Feng Zhang

   Article

   Kreitz et al Nature. 2023 Apr;616(7956):357-364. doi:

   Insert

   pHCMV-SS-antiMoonTagNb-TM
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pHCMV-SS-antiALFAtagNb-TM

   Plasmid

   #198307

   Purpose

   Displays anti-ALFA nanobody from the surface of mammalian cells

   Depositor

   Feng Zhang

   Article

   Kreitz et al Nature. 2023 Apr;616(7956):357-364. doi:

   Insert

   pHCMV-SS-antiALFAtagNb-TM
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pHEA

   Plasmid

   #168297

   Purpose

   Bacterial surface display of polypeptides (nanobodies) in E. coli

   Depositor

   Luis Ángel Fernández

   Article

   Marin et al J Bacteriol. 2010 Nov;192(21):5588-602.

   Insert

   Enterohemorraghic E. coli autotransporter EhaA C-terminal fragment (C-EhaA)
   

   Use

   Tags

   6xHis, E-tag, and PelB signal peptide

   Expression

   Bacterial

   Mutation

   Promoter

   LacI-Plac

   Available since

   June 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pTREX GPR68_NB2_2

   Plasmid

   #127691

   Purpose

   Plasmid contains a nanobody that directly interacts with the cytoplasmic surface of GPR68.

   Depositor

   Justin English, Bryan Roth

   Article

   English et al Cell. 2019 Jul 2. pii: S0092-8674(19)306

   Insert

   GPR68_NB2_2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pHRW-CRY2olig-VHH(GFP)

   Plasmid

   #126023

   Purpose

   express RFP-tagged CRY2 PHR E490G fused to anti-GFP nanobody for optogenetics

   Depositor

   Eurico Morais-de-Sá

   Article

   Osswald et al Biomolecules. 2019 Feb 12;9(2). pii: bio

   Insert

   CRY2 PHRolig-VHH(GFP)
   

   Use

   Tags

   RFP

   Expression

   Insect

   Mutation

   CRY2 E490G

   Promoter

   Hsp70

   Available since

   Sept. 24, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. mCh-LaGFP(N)-cpFRB2-T2A-FKBP-LaGFP(C)

   Plasmid

   #165030

   Purpose

   Express cpFRB2-based GFP specific Chessbody (chemically switchable split nanobody); visualized by mCherry

   Depositor

   Yubin Zhou

   Article

   Lee et al J Mol Biol. 2020 May 1;432(10):3127-3136

   Insert

   LaGFP(N)-cpFRB2-T2A-FKBP-LaGFP(C)
   

   Use

   Tags

   mCherry

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   T7

   Available since

   May 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pHRW-CRY2-VHH(GFP)

   Plasmid

   #126022

   Purpose

   express RFP-tagged CRY2 PHR domain fused to anti-GFP nanobody for optogenetics

   Depositor

   Eurico Morais-de-Sá

   Article

   Osswald et al Biomolecules. 2019 Feb 12;9(2). pii: bio

   Insert

   CRY2 PHR-VHH(GFP)
   

   Use

   Tags

   RFP

   Expression

   Insect

   Mutation

   WT

   Promoter

   Hsp70

   Available since

   Sept. 24, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. mAID(KR)–p53 NB(KR)

   Plasmid

   #232300

   Purpose

   KR p53 adaptor

   Depositor

   Masato Kanemaki

   Article

   Islam et al J Cell Sci. 2025 Jun 9:jcs.263961. doi:

   Inserts

   KR p53 nanobody adaptor
   OsTIR1(F74G)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. mCh-LaGFP(N)-cpFRB1-T2A-FKBP-LaGFP(C)

   Plasmid

   #165029

   Purpose

   Express cpFRB1-based GFP specific Chessbody (chemically switchable split nanobody); visualized by mCherry

   Depositor

   Yubin Zhou

   Article

   Lee et al J Mol Biol. 2020 May 1;432(10):3127-3136

   Insert

   LaGFP(N)-cpFRB1-T2A-FKBP-LaGFP(C)
   

   Use

   Tags

   mCherry

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   T7

   Available since

   May 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. GFP-LaM(N)-cpFRB1-T2A-FKBP-LaM(C)

    Plasmid

    #165026

    Purpose

    Express cpFRB1-based mCherry specific Chessbody (chemically switchable split nanobody); visualized by GFP

    Depositor

    Yubin Zhou

    Article

    Lee et al J Mol Biol. 2020 May 1;432(10):3127-3136

    Insert

    LaM8(N)-cpFRB1-T2A-FKBP-LaM8(C)
    

    Use

    Tags

    GFP

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    T7

    Available since

    May 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. GFP-LaM(N)-cpFRB2-T2A-FKBP-LaM(C)

    Plasmid

    #165028

    Purpose

    Express cpFRB2-based mCherry specific Chessbody (chemically switchable split nanobody); visualized by GFP

    Depositor

    Yubin Zhou

    Article

    Lee et al J Mol Biol. 2020 May 1;432(10):3127-3136

    Insert

    LaM8(N)-cpFRB2-T2A-FKBP-LaM8(C)
    

    Use

    Tags

    GFP

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    T7

    Available since

    May 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3.1 5HT2A_NB2_1

    Plasmid

    #127690

    Purpose

    Plasmid contains a positive allosteric modulating nanobody the binds to the cytoplasmic surface of 5HT2A.

    Depositor

    Justin English, Bryan Roth

    Article

    English et al Cell. 2019 Jul 2. pii: S0092-8674(19)306

    Insert

    5HT2A_NB2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET21b-LaG9(S151C)

    Plasmid

    #172490

    Purpose

    Encodes the anti-GFP LaG-9 nanobody with a cysteine near the C-terminus, LaG-9(S151C).

    Depositor

    Siegfried Musser

    Article

    Chowdhury et al Nat Cell Biol. 2022 Jan;24(1):112-122. d

    Insert

    LaG9(S151C)
    

    Use

    Tags

    6xHis, PPX cleavage site, and pelB leader sequenc…

    Expression

    Bacterial

    Mutation

    S151C

    Promoter

    T7

    Available since

    March 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pHR Ef1a_LaG16-synNotch_tTA-PGK_Puro

    Plasmid

    #223536

    Purpose

    Anti-GFP LaG16 nanobody-based synNotch; puro-selectable

    Depositor

    Jonathan Brunger

    Article

    Walton et al Osteoarthritis Cartilage. 2025 Feb;33(2)

    Insert

    LaG16_synNotch_tTA
    

    Use

    Lentiviral

    Tags

    c-myc tag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 17, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. Nb-gp41-Halo (MoonTag-Nb-Halo)

    Plasmid

    #128603

    Purpose

    Expresses nanobody-2H10 fused to Halo in mammalian cells. It recognizes gp41 peptide array (MoonTag peptide array).

    Depositor

    Marvin Tanenbaum

    Article

    Boersma et al Cell. 2019 Jun 5. pii: S0092-8674(19)304

    Insert

    Nanobody_gp41
    

    Use

    Lentiviral

    Tags

    GB1 and Halo

    Expression

    Mutation

    Promoter

    SFFV

    Available since

    Aug. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pcDNA3-endo-Nb80

    Plasmid

    #205518

    Purpose

    Endosome-targeted Nb80 nanobody that binds and stabilizes β2AR active conformation, blocking Gαs coupling.

    Depositor

    Jin Zhang

    Article

    Kwon et al Nature. 2022 Nov;611(7934):173-179. doi:

    Insert

    endo-Nb80
    

    Use

    Tags

    2xFYVE (Fab1/YOTB/Vac1/EEA1 zinc-finger) and mChe…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pcDNA3-endo-Nb37

    Plasmid

    #205514

    Purpose

    Endosome-targeted Nb37 nanobody that binds and stabilizes the active form of Gαs G-protein subunit.

    Depositor

    Jin Zhang

    Article

    Kwon et al Nature. 2022 Nov;611(7934):173-179. doi:

    Insert

    endo-Nb37
    

    Use

    Tags

    2xFYVE (Fab1/YOTB/Vac1/EEA1 zinc-finger) and mChe…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. Nb-gp41-GFP (MoonTag-Nb-GFP)

    Plasmid

    #128602

    Purpose

    Expresses nanobody-2H10 fused to super folded GFP (sfGFP) in mammalian cells. It recognizes gp41 peptide array (MoonTag peptide array).

    Depositor

    Marvin Tanenbaum

    Article

    Boersma et al Cell. 2019 Jun 5. pii: S0092-8674(19)304

    Insert

    Nanobody_gp41
    

    Use

    Lentiviral

    Tags

    GB1 and sfGFP

    Expression

    Mutation

    Promoter

    SFFV

    Available since

    Oct. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pRDA_886

    Plasmid

    #201162

    Purpose

    Lentiviral expression of EnAsCas12a CRISPRa gRNA with N' nanobody-ALFA and C' VP64 and NLS

    Depositor

    John Doench

    Article

    Griffith et al Cell Genom. 2023 Sep 1;3(9):100387. doi:

    Insert

    Puromycin resistance
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    EFS

    Available since

    June 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pVDL9.3

    Plasmid

    #168299

    Purpose

    Secretion of polypeptides (nanobodies) in E. coli fused to C-terminal secretion signal of HlyA

    Depositor

    Víctor de Lorenzo

    Article

    Tzschaschel et al Nat Biotechnol. 1996 Jun;14(6):765-9. do

    Insert

    Segment of the hly operon, spanning the C-terminal part of hlyA, hlyB, and hlyD
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    LacI-Plac

    Available since

    June 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge