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A*24:07:01

Plasmid

#203283

Purpose

Overexpress HLA allele

Depositor

Derin Keskin, Catherine Wu

Article

Sarkizova et al Nat Biotechnol. 2020 Feb;38(2):199-209.

Insert

A*24:07:01 (HLA-A Human)

Expression

Mammalian

Available Since

July 10, 2023

Availability

Academic Institutions and Nonprofits only

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pCG_SARS-CoV2 NSP16 C-V5-tag

Plasmid

#179976

Purpose

Mammalian expression vector for SARS-CoV-2 Nsp16, V5-tagged. Sequence codon-optimized.

Depositor

Konstantin Sparrer

Article

Hayn et al Cell Rep. 2021 May 18;35(7):109126.

Insert

SARS-CoV-2 Nsp16 (ORF1ab Synthetic)

Expression

Mammalian

Mutation

human codon-optimized

Available Since

Feb. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pmCherry-C-EP300

Plasmid

#166454

Purpose

Expresses fusion of mCherry and EP300-C-IDR

Depositor

Shuguo Sun

Article

Yu et al Mol Cell. 2021 Feb 13. pii: S1097-2765(2

Insert

mCherry, C-EP300(1795-2414) (EP300 Human)

Expression

Mammalian

Available Since

April 9, 2021

Availability

Academic Institutions and Nonprofits only

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pmCherry-N-EP300

Plasmid

#166452

Purpose

Expresses fusion of mCherry and EP300-N-IDR

Depositor

Shuguo Sun

Article

Yu et al Mol Cell. 2021 Feb 13. pii: S1097-2765(2

Insert

mCherry, N-EP300(1-341) (EP300 Human)

Expression

Mammalian

Available Since

April 9, 2021

Availability

Academic Institutions and Nonprofits only

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iGFP-gamma-actin

Plasmid

#231554

Purpose

Mammalian expression of human gamma actin fused intramolecularly to monomeric superfolder GFP

Depositor

Manos Mavrakis

Article

Martins et al Cell. 2025 Apr 1:S0092-8674(25)00276-4.

Insert

ACTG1 (ACTG1 Human)

Tags

msfGFP (intramolecular)

Expression

Mammalian

Promoter

CMV

Available Since

March 19, 2025

Availability

Academic Institutions and Nonprofits only

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pTRE3G-GSX2-IRES2-EBF1

Plasmid

#96966

Purpose

Dox-inducible expression of Gsx2 and Ebf1 in mammalian cells

Depositor

Elena Cattaneo

Article

Faedo et al Proc Natl Acad Sci U S A. 2017 Feb 14;11

Insert

GSX2 and EBF1 (bicistronic insert) (Gsx2, Ebf1 Mouse)

Tags

FLAG (on EBF1)

Expression

Mammalian

Available Since

July 31, 2017

Availability

Academic Institutions and Nonprofits only

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pCMVtrunc sfCherry2ΔC4-Lifeact

Plasmid

#231557

Purpose

Mammalian expression of Lifeact fused to C-terminally truncated superfolder Cherry2, for actin filament organization measurements using fluorescence polarization microscopy

Depositor

Manos Mavrakis

Article

Martins et al Cell. 2025 Apr 1:S0092-8674(25)00276-4.

Insert

Lifeact (ABP140 Budding Yeast)

Tags

sfCherry2

Expression

Mammalian

Promoter

CMVtrunc

Available Since

Jan. 30, 2025

Availability

Academic Institutions and Nonprofits only

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pNTK human MBD3 PGK-Neo-pA flox

Plasmid

#52362

Purpose

Targeting vector for Human MBD3 conditional knockout. Also creates hypomorphic alleles.

Depositor

Jacob Hanna

Article

Rais et al Nature. 2013 Oct 3;502(7469):65-70. doi:

Insert

MBD3 (MBD3 Human)

Use

Targeting

Available Since

Sept. 8, 2014

Availability

Academic Institutions and Nonprofits only

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pCG_SARS-CoV2 ORF3a C-V5-tag

Plasmid

#179980

Purpose

Mammalian expression vector for SARS-CoV-2 ORF3a, V5-tagged. Sequence codon-optimized.

Depositor

Konstantin Sparrer

Article

Hayn et al Cell Rep. 2021 May 18;35(7):109126.

Insert

SARS-CoV-2 ORF3a (ORF3a Synthetic)

Expression

Mammalian

Mutation

human codon-optimized

Available Since

Feb. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pDEST40-mPTER

Plasmid

#224864

Purpose

Mammalian expression mouse PTER protein

Depositor

Jonathan Long

Article

Wei et al bioRxiv [Preprint]. 2024 Mar 22:2024.03.

Insert

mouse PTER (Pter Mouse)

Tags

C-term v5 and His tag

Expression

Mammalian

Available Since

Oct. 9, 2024

Availability

Academic Institutions and Nonprofits only

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B*40:01:01

Plasmid

#203257

Purpose

Overexpress HLA allele

Depositor

Derin Keskin, Catherine Wu

Article

Sarkizova et al Nat Biotechnol. 2020 Feb;38(2):199-209.

Insert

B*40:01:01 (HLA-B Human)

Expression

Mammalian

Available Since

July 10, 2023

Availability

Academic Institutions and Nonprofits only

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pLKO.1.shCDK8

Plasmid

#19760

Depositor

William Hahn

Article

Firestein et al Nature. 2008 Sep 25. 455(7212):547-51.

Insert

small hairpin RNA against CDK8 (CDK8 Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

Oct. 27, 2008

Availability

Academic Institutions and Nonprofits only
pCG_batCoV RaTG13 N C-V5-tag

Plasmid

#179987

Purpose

Mammalian expression vector for RaTG13 N, V5-tagged. Sequence codon-optimized.

Depositor

Konstantin Sparrer

Article

Hayn et al Cell Rep. 2021 May 18;35(7):109126.

Insert

RaTG13 N

Expression

Mammalian

Mutation

human codon-optimized

Available Since

Feb. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pCG_SARS-CoV1 N C-V5-tag

Plasmid

#179968

Purpose

Mammalian expression vector for SARS-CoV-1 N, V5-tagged. Sequence codon-optimized.

Depositor

Konstantin Sparrer

Article

Hayn et al Cell Rep. 2021 May 18;35(7):109126.

Insert

SARS-CoV-1 N (N Synthetic)

Expression

Mammalian

Mutation

human codon-optimized

Available Since

Feb. 4, 2022

Availability

Academic Institutions and Nonprofits only

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FUW-Flag-Mbd3

Plasmid

#52371

Purpose

Lentiviral expression vector for constitutive expression of double Flag tagged Mbd3

Depositor

Jacob Hanna

Article

Rais et al Nature. 2013 Oct 3;502(7469):65-70. doi:

Insert

Mbd3 (Mbd3 Mouse)

Use

Lentiviral

Tags

flag

Expression

Mammalian

Promoter

Ub

Available Since

Sept. 8, 2014

Availability

Academic Institutions and Nonprofits only

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pCG_batCoV RaTG13 ORF3a C-V5-tag

Plasmid

#179988

Purpose

Mammalian expression vector for RaTG13 ORF3a, V5-tagged. Sequence codon-optimized.

Depositor

Konstantin Sparrer

Article

Hayn et al Cell Rep. 2021 May 18;35(7):109126.

Insert

RaTG13 ORF3a

Expression

Mammalian

Mutation

human codon-optimized

Available Since

Feb. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pCG_SARS-CoV1 NSP3 C-V5-tag

Plasmid

#179962

Purpose

Mammalian expression vector for SARS-CoV-1 Nsp3, V5-tagged. Sequence codon-optimized.

Depositor

Konstantin Sparrer

Article

Hayn et al Cell Rep. 2021 May 18;35(7):109126.

Insert

SARS-CoV-1 Nsp3 (ORF1ab Synthetic)

Expression

Mammalian

Mutation

human codon-optimized

Available Since

Feb. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pLVX-2xFLAG-2xSTREP-CCND1(P287A)-IRES-mCherry

Plasmid

#172637

Purpose

Lentiviral bicistronic vector for the constitutive co-expression of 2xFLAG-2xSTREP-tagged cyclin D1(P287A) and mCherry in mammalian cells

Depositor

Michele Pagano

Article

Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

Insert

CCND1 (CCND1 Human)

Use

Lentiviral

Tags

2xFLAG-2xSTREP

Expression

Mammalian

Mutation

Pro287Ala

Promoter

CMV

Available Since

Aug. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pUC19-YAP-mEGFP

Plasmid

#166472

Purpose

HDR template for tagging of endogenous mouse YAP N-terminus with mEGFP

Depositor

Shuguo Sun

Article

Yu et al Mol Cell. 2021 Feb 13. pii: S1097-2765(2

Insert

YAP-mEGFP HDR template(mouse Yap) (Yap1 Mouse)

Expression

Mammalian

Available Since

May 13, 2021

Availability

Academic Institutions and Nonprofits only

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pMSCV-FLAG-2xSTREP-CCND3(T283A)-Puro

Plasmid

#172618

Purpose

Retroviral vector for the constitutive expression of FLAG-2xSTREP-tagged cyclin D3(T283A) and a puromycin resistance marker in mammalian cells

Depositor

Michele Pagano

Article

Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

Insert

CCND3 (CCND3 Human)

Use

Retroviral

Tags

FLAG-2xSTREP

Expression

Mammalian

Mutation

Thr283Ala

Available Since

Aug. 20, 2021

Availability

Academic Institutions and Nonprofits only

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