176,110 results
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Plasmid#207858PurposeMammalian expression of PEmaxDRNaseH prime editorDepositorInsertPEmaxDRNaseH
TagsSV40 bpNLS and SV40 bpNLS, c-Myc NLSExpressionMammalianMutationM-MLVRTDRNAseHrtAvailable sinceSept. 27, 2023AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pTS117 His14-Avi-45xGS-anti GFP nanobody
Plasmid#199370PurposeBacterial expression plasmid for anti-GFP nanobody with an N-terminal His-tag and biotin acceptor peptide (Avi)DepositorInsertanti-GFP nanobody
Tags14xHis-AviExpressionBacterialMutationWTPromoterT5-LacOAvailable sinceApril 21, 2023AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pDEST_FLAG-HUWE1
Plasmid#187120Purposemammalian cell expression of FLAG-HUWE1DepositorAvailable sinceNov. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
AAV-EFS-SaKKHABE8e-bGH-U6-sgRNA-BsmBI
Plasmid#189923PurposeAAV genome encoding SaKKHABE8e and Sa sgRNA cassette on a single AAV, with BsmBI sites for protospacer installationDepositorInsertSaABE8e V106W
UseAAVMutationnSaCas9 KKH, TadA ABE8e V106WPromoterEFSAvailable sinceSept. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
psfGFP-LAMP1-mCherry (pHLARE)
Plasmid#164477PurposeLysosomal pH biosensor consisting of sfGFP-rat Lamp1-mCherry.DepositorAvailable sinceSept. 9, 2022AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pLminP_Luc2P_RE5
Plasmid#90339PurposeSTAT3 - Stat3 homodimer gene reporterDepositorInsertFirefly luciferase
UseLentiviralPromoterSTAT3Available sinceAug. 22, 2022AvailabilityAcademic Institutions and Nonprofits onlyCited by -
LentiGuide-puro-NLS-mCherry
Plasmid#185474PurposeMammalian Expression, Lentiviral vector expressing mCherry for two-colour competitive growth assay. Clone guide oligos into BsmBI site.DepositorTypeEmpty backboneUseLentiviralTagsmCherryExpressionMammalianPromoterCMVAvailable sinceJuly 1, 2022AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pcDNA3.1+_hsTRPA1_myc_dTom
Plasmid#183179Purposemyc tagged hsTRPA1 used for cell transfectionDepositorInsertsExpressionMammalianAvailable sinceMay 26, 2022AvailabilityAcademic Institutions and Nonprofits onlyCited by -
p5154
Plasmid#175390PurposeHigh Copy Number DNA Donor Vector for Bxb1 Integrase-mediated Cassette Exchange (for inserts <15kb) into dual site mouse strains (Bxb-GT/GA)DepositorTypeEmpty backboneUseMouse Targeting; Cloning vector, to be used to de…Available sinceMarch 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-dCasRx-ALKBH5-PURO
Plasmid#175582PurposeExpresses dCasRx-ALKBH5 in mammalian cellDepositorInsertdCasRx-ALKBH5
UseCRISPR and LentiviralTagsFLAG-HAExpressionMammalianMutationRfxCas13d R239A/H244A/R858A/H863APromoterpMSCVAvailable sinceNov. 5, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pcDNA4TO-SCN8A-Variant-1-IRES-mScarlet
Plasmid#162280PurposeEukaryotic expression of human SCN8A variant 1 isoform. This channel has been modified to be stably maintained in standard bacterial strains.DepositorInsertSCN8A Variant 1, stabilized (SCN8A Human)
TagsIRES-mScarletExpressionMammalianMutationModified human HSPA5 intron 4 inserted after bp23…PromoterCMVAvailable sinceJune 10, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pET51b-His-TEV-HaloTag7
Plasmid#167266PurposeOverexpression of HaloTag7 in E. coliDepositorInsertHaloTag7
TagsHis x10ExpressionBacterialPromoterT7Available sinceApril 14, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCMV-2NLS-Cas9-6XMS2
Plasmid#166033PurposeExpression of 6X MS2 stem loops fused SpCas9 mRNA for packaging of SpCas9 mRNA within lentiviral particles.DepositorInsertSpCas9
UseCRISPR and LentiviralPromoterCMVAvailable sinceMarch 25, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pcDNA3.1 SARS-CoV-2 S D614G
Plasmid#158075PurposeExpresses SARS-CoV-2 spike protein with a D614G mutationDepositorAvailable sinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pXG260
Plasmid#141282PurposeCMV:EGFP (as control for pXG237)DepositorInsertEGFP
UseLentiviralPromoterCMVAvailable sinceSept. 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
5xUAS:GAP-EGFP-P2A-NfsB_Vv F70A/F108Y,he:tagBFP2
Plasmid#158652Purposemaking transgenics expressing a UAS driven eGFP and NTR 2.0DepositorInsert5xUAS:GAP-eGFP-P2A-NfsB_Vv F70A/F108Y;he:BFP
UseTol2-based transgenic expression in zebrafish/oth…MutationF70A/F108YAvailable sinceSept. 14, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pET_RTX(exo-)-6xHis
Plasmid#145028PurposepET vector for IPTG-inducible, T7-based expression of the exonuclease deficient version of RTX bearing a C-terminal 6xHis tagDepositorPublicationInsertRTX(exo-)
Tags6xHisExpressionBacterialMutationN210DPromoterT7Available sinceMay 7, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pGBW-m4046887
Plasmid#145730PurposeBacterial Expression plasmid for SARS-CoV-2 surface glycoprotein (Spike protein)DepositorInsertSARS-CoV-2 S (surface glycoprotein) (S Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
TagsCleavable TEV;6xHISExpressionBacterialMutationwtPromoterPromoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…Available sinceApril 28, 2020AvailabilityIndustry, Academic Institutions, and NonprofitsCited by -
pemiRFP703-N1
Plasmid#136558PurposeA cytoplasmic expression of a monomeric phytochrome-based near-infrared fluorescent protein emiRFP703 (enhanced miRFP703)DepositorInsertemiRFP703
ExpressionMammalianPromoterCMVAvailable sinceFeb. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-6XHis-human SUMO 1
Plasmid#133770Purposeexpress 6Xhis- SUMO 1 proteinDepositorAvailable sinceDec. 6, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by