We narrowed to 18,937 results for: Tec

1. PKN3 gRNA (BRDN0001146747)

   Plasmid

   #77949

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human PKN3

   Depositor

   John Doench, David Root

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   PKN3 (PKN3 Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. PKN3 gRNA (BRDN0001487150)

   Plasmid

   #77950

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human PKN3

   Depositor

   John Doench, David Root

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   PKN3 (PKN3 Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAd5-Blue

   Plasmid

   #174431

   Purpose

   pAd5-Blue provides a platform for the rapid construction of recombinant and replication defective Adenovirus. This vector contains unique restriction enzyme sites to allow cloning of a gene.

   Depositor

   Teresa De Los Santos

   Article

   Fernandez-Sainz et al Virology. 2017 Feb;502:123-132. doi: 10.

   Insert

   β-galactosidase α gene fragment
   

   Use

   Adenoviral

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 18, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCDNA_B2AR-no-pep

   Plasmid

   #47439

   Purpose

   control FRET sensor for beta2-adrenergic receptor

   Depositor

   Sivaraj Sivaramakrishnan

   Article

   Malik et al J Biol Chem. 2013 Jun 14;288(24):17167-7

   Insert

   beta2-adrenergic receptor (ADRB2 Human)
   

   Tags

   10 nm ER/K helix, HA, mCerulean, mCitrine, and no…

   Expression

   Mammalian

   Promoter

   pCMV

   Available Since

   Sept. 11, 2013

   Availability

   Academic Institutions and Nonprofits only

5. A*03:02:01

   Plasmid

   #203294

   Purpose

   Overexpress HLA allele

   Depositor

   Derin Keskin, Catherine Wu

   Article

   Sarkizova et al Nat Biotechnol. 2020 Feb;38(2):199-209.

   Insert

   A*03:02:01 (HLA-A Human)
   

   Expression

   Mammalian

   Available Since

   July 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLenti myc-NL-GW

   Plasmid

   #113454

   Purpose

   Lentiviral NanoLuc Gateway shuttle vector for N-terminal fusions

   Depositor

   Erich Wanker

   Article

   Trepte et al Mol Syst Biol. 2018 Jul 11;14(7):e8071.

   Type

   Empty backbone

   Use

   Lentiviral and Luciferase

   Tags

   cmyc-NanoLuc

   Expression

   Mammalian

   Promoter

   hUbC

   Available Since

   Jan. 16, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLenti-TRE3G-BE3-PGK-Puro

   Plasmid

   #110845

   Purpose

   Lentiviral vector for dox-inducible expression of BE3 (not codon optimized)

   Depositor

   Lukas Dow

   Article

   Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

   Insert

   BE3
   

   Use

   Lentiviral

   Mutation

   D10A

   Promoter

   TRE3G

   Available Since

   Aug. 23, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA-Flag-LmoCas6

   Plasmid

   #126485

   Purpose

   Encodes L.monocytogenes CRISPR Type I-B Cas6 with N-terminal fusion of Flag tag epitope and nuclear localization signal driven by CMV promoter

   Depositor

   Charles Gersbach

   Article

   Pickar-Oliver et al Nat Biotechnol. 2019 Sep 23. pii: 10.103

   Insert

   LmoCas6
   

   Use

   CRISPR

   Tags

   Flag, NLS

   Expression

   Mammalian

   Promoter

   CMV and U6

   Available Since

   Sept. 30, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. shMEK1/2 v2 neo

   Plasmid

   #72570

   Purpose

   Hairpin targeting MEK1 and MEK2 (murine).

   Depositor

   Kevin Janes

   Article

   Jensen et al Cell Syst. 2016 Feb 24;2(2):112-121.

   Insert

   MEK1/2 (Map2k1, Map2k2 Mouse)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   July 12, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pDEST_FLAG-HUWE1_UBM-mut

    Plasmid

    #187150

    Purpose

    mammalian cell expression of FLAG-HUWE1 L2976A/P2977A

    Depositor

    Eric Fischer

    Article

    Hunkeler et al Mol Cell. 2021 Sep 2;81(17):3468-3480.e7

    Insert

    HUWE1 (HUWE1 Human)
    

    Tags

    1xFLAG

    Expression

    Mammalian

    Mutation

    L2976A/P2977A

    Available Since

    Aug. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pDEST_FLAG-HUWE1_UIM-mut

    Plasmid

    #187148

    Purpose

    mammalian cell expression of FLAG-HUWE1 A1376G/S1382A

    Depositor

    Eric Fischer

    Article

    Hunkeler et al Mol Cell. 2021 Sep 2;81(17):3468-3480.e7

    Insert

    HUWE1 (HUWE1 Human)
    

    Tags

    1xFLAG

    Expression

    Mammalian

    Mutation

    A1376G/S1382A

    Available Since

    Aug. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pDEST_FLAG-HUWE1_UBA-mut

    Plasmid

    #187146

    Purpose

    mammalian cell expression of FLAG-HUWE1 M1328A/F1330A

    Depositor

    Eric Fischer

    Article

    Hunkeler et al Mol Cell. 2021 Sep 2;81(17):3468-3480.e7

    Insert

    HUWE1 (HUWE1 Human)
    

    Tags

    1xFLAG

    Expression

    Mammalian

    Mutation

    M1328A/F1330A

    Available Since

    Aug. 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET32a-Spike-S-4P

    Plasmid

    #165094

    Purpose

    protein expression in E. coli

    Depositor

    Rick Tarleton

    Article

    Shen et al medRxiv [Preprint]. 2021 Jan 27:2021.01.

    Insert

    Severe acute respiratory syndrome coronavirus 2 spike protein site 550-570, 785- 805, 810-830 and 1146 1166 (S SARS-CoV2)
    

    Expression

    Bacterial

    Available Since

    March 2, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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14. pYB-Dual(mNG(yeast-opt))

    Plasmid

    #216727

    Purpose

    Dual-species protein expression vector for high-level and inducible protein expression of mNeonGreen (codon optimized for yeast) in both yeast and bacteria from the same construct.

    Depositor

    Emil Jensen

    Article

    Waneskog et al Biotechnol Prog. 2024 May 17:e3482. doi:

    Insert

    mNeonGreen
    

    Expression

    Bacterial and Yeast

    Promoter

    pGAL1(yeast) and pT7-RNAP(bacteria)

    Available Since

    May 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pINTO-NSA::hSNRNP70

    Plasmid

    #61726

    Purpose

    Expression of human SNRNP70 fused to monomeric streptavidin (N-terminus)

    Depositor

    Roberto Bonasio

    Article

    Beck et al J Proteome Res. 2014 Dec 5;13(12):6135-4

    Insert

    SNRP70 (SNRNP70 Human)
    

    Tags

    mSA (monomeric streptavidin)

    Expression

    Mammalian

    Mutation

    Two silent mutations to ensure restriction site c…

    Promoter

    T-REx (CMV + 2xTetO2)

    Available Since

    Jan. 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. Polymerase expression - pCMV-Phi29 (LM2708)

    Plasmid

    #208965

    Purpose

    Unfused Phi29 DNA polymerase (-exo), expressed from CMV or T7 promoters.

    Depositor

    Benjamin Kleinstiver

    Article

    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

    Insert

    Phi29-BPNLS
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS

    Expression

    Mammalian

    Mutation

    Phi29(-exo;D169A)

    Promoter

    CMV and T7

    Available Since

    Nov. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET-pro-siRNA-V2-TP53

    Plasmid

    #111089

    Purpose

    pET-pro-siRNA-V2 based plasmid for production of recombinant siRNAs (pro-siRNAs) against human TP53 gene.

    Depositor

    Linfeng HUANG

    Article

    Kaur et al Biotechnol Bioeng. 2018 Jun 5. doi: 10.1

    Insert

    TP53 (TP53 Human)
    

    Expression

    Bacterial

    Available Since

    June 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCMV-hA3A-BE3-Y132D

    Plasmid

    #113425

    Purpose

    Expresses hA3A-BE3-Y132D in mammalian cells

    Depositor

    Jia Chen

    Article

    Wang et al Nat Biotechnol. 2018 Aug 20. pii: nbt.41

    Insert

    hA3A-BE3-Y132D (APOBEC3A Human, S. pyogenes and Bacteriophage PBS2)
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    hAPOBEC3A_Y132D

    Promoter

    CMV

    Available Since

    Aug. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCALNL-Kir2.1

    Plasmid

    #125578

    Purpose

    Cre-dependent expression of Kir2.1 (FLAG-tagged) in mammalian cells.

    Depositor

    Takeshi Imai

    Article

    Fujimoto et al Dev Cell. 2023 Jul 24;58(14):1221-1236.e

    Insert

    Kir2.1 (Kcnj2 Mouse)
    

    Use

    Cre/Lox

    Tags

    FLAG

    Expression

    Mammalian

    Available Since

    July 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA-Flag-LmoCas8b2

    Plasmid

    #126482

    Purpose

    Encodes L.monocytogenes CRISPR Type I-B Cas8b2 with N-terminal fusion of Flag tag epitope and nuclear localization signal driven by CMV promoter

    Depositor

    Charles Gersbach

    Article

    Pickar-Oliver et al Nat Biotechnol. 2019 Sep 23. pii: 10.103

    Insert

    LmoCas8b2
    

    Use

    CRISPR

    Tags

    Flag, NLS

    Expression

    Mammalian

    Promoter

    CMV and U6

    Available Since

    Sept. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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